scholarly journals Photosensitizers Used in the Photodynamic Therapy of Rheumatoid Arthritis

2019 ◽  
Vol 20 (13) ◽  
pp. 3339 ◽  
Author(s):  
Manuel Gallardo-Villagrán ◽  
David Yannick Leger ◽  
Bertrand Liagre ◽  
Bruno Therrien
Keyword(s):  
Rheumatoid Arthritis ◽  
Photodynamic Therapy ◽  
Light Irradiation ◽  
Excitation Wavelength ◽  
Target Tissue ◽  
Non Invasive ◽  
Different Types ◽  
Invasive Method ◽  
Promising Treatment ◽  
As Solubility

Photodynamic Therapy (PDT) has become one of the most promising treatment against autoimmune diseases, such as rheumatoid arthritis (RA), as well as in the treatment of different types of cancer, since it is a non-invasive method and easy to carry out. The three main ingredients of PDT are light irradiation, oxygen, and a photosensitizer (PS). Light irradiation depends on the type of molecule or compound to be used as a PS. The concentration of O2 fluctuates according to the medium where the target tissue is located and over time, although it is known that it is possible to provide oxygenated species to the treated area through the PS itself. Finally, each PS has its own characteristics, the efficacy of which depends on multiple factors, such as solubility, administration technique, retention time, stability, excitation wavelength, biocompatibility, and clearance, among others. Therefore, it is essential to have a thorough knowledge of the disease to select the best PS for a specific target, such as RA. In this review we will present the PSs used in the last three decades to treat RA under PDT protocol, as well as insights on the relevant strategies.

scholarly journals A series of photosensitizers with incremental positive electric charges for photodynamic antitumor therapy

RSC Advances ◽  
2019 ◽  
Vol 9 (42) ◽  
pp. 24560-24567 ◽  
Author(s):  
Shufeng Yan ◽  
Qingqing Huang ◽  
Xiaorong Song ◽  
Zhuo Chen ◽  
Mingdong Huang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Antitumor Therapy ◽  
Non Invasive ◽  
Invasive Method

In recent years, photodynamic therapy (PDT) studies have gained considerable attention as a non-invasive method used to fight cancer.

scholarly journals Non Invasive Treatment to Relieve the Pain due to Headaches & Sinus using Vibration on Acupressure Points

2019 ◽  
Vol 9 (1S4) ◽  
pp. 585-588
Keyword(s):  
Stress Condition ◽  
Cost Effective ◽  
Climatic Changes ◽  
Invasive Treatment ◽  
Non Invasive ◽  
Common Term ◽  
Different Types ◽  
Invasive Method ◽  
Frequency Changes ◽  
Head Neck

Headache is a common term that causes pain in the region of head, neck, and scalp. It is a neurological disorder. The nerves are tending to be in under stress condition. The main causes of the headache are due to stress, depression, tension, anxiety and due to some climatic changes, etc. Headache is classified into different types. Migraine, cluster headache, sinus, normal headache are some of the types of headache. Most headaches can be treated with some medication and neuro stimulator devices. This paper involves the usage of vibration by non-invasive method on the acupressure point of the human body which can relief the pain. The acupressure point is a pressure point, when used in a correct method will lead to decrease the level of pain. The vibration is provided by the motor vibrator and the frequency of the vibration is controlled by the arduino (Atmega 328). Frequency changes can be monitored by the pc. This vibration based treatment will effectively reduce the pain and also the device is cost effective and more comfortable to the patient.

scholarly journals Optical coherence tomography as a non-invasive method of enamel thickness diagnosis after orthodontic treatment by 3 different types of brackets

2019 ◽  
Vol 28 (2) ◽  
pp. 211-218 ◽  
Author(s):  
Monika Machoy ◽  
Robert Koprowski ◽  
Liliana Szyszka-Sommerfeld ◽  
Krzysztof Safranow ◽  
Tomasz Gedrange ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Orthodontic Treatment ◽  
Optical Coherence ◽  
Enamel Thickness ◽  
Non Invasive ◽  
Different Types ◽  
Invasive Method

scholarly journals In situCharacterisation of Living Cells by Raman Spectroscopy

2002 ◽  
Vol 16 (2) ◽  
pp. 43-51 ◽  
Author(s):  
I. Notingher ◽  
S. Verrier ◽  
H. Romanska ◽  
A. E. Bishop ◽  
J. M. Polak ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Laser Excitation ◽  
Living Cells ◽  
Fused Silica ◽  
Excitation Wavelength ◽  
Bioactive Scaffolds ◽  
Non Invasive ◽  
Invasive Method ◽  
45S5 Bioglass

We report the first Raman spectra of individual living and dead cells (MLE-12 line) cultured on bioinert standard poly-L-lysine coated fused silica and on bioactive 45S5 Bioglass®measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to the cells even after 40 minutes irradiation at 115 mW power, as indicated by cell morphology observation and trypan blue viability test. We show that shorter wavelength lasers, 488 nm and 514 nm, cannot be used because they induce damage to the cells at very low laser powers (5 mW) and short irradiation times (5–20 minutes). The most important differences between the spectra of living and dead cells are in the 1530–1700 cm−1range, where the dead cells have strong peaks at 1578 cm−1and 1607 cm−1. Other differences occur around the DNA peak at 1094 cm−1. Our study establishes the feasibility of using the 785 nm laser for anin situreal-time non-invasive method to follow biological events (proliferation, differentiation, cell death, etc.) within individual cells cultured on bioactive scaffolds in their physiologic environment over long periods of time.

scholarly journals Measurement of Fecal Testosterone Metabolites in Mice: Replacement of Invasive Techniques

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 165 ◽  
Author(s):  
Kerstin E. Auer ◽  
Marius Kußmaul ◽  
Erich Möstl ◽  
Katharina Hohlbaum ◽  
Thomas Rülicke ◽  
...  
Keyword(s):  
Fecal Samples ◽  
Non Invasive ◽  
Different Types ◽  
Invasive Method ◽  
Males And Females ◽  
Diurnal Fluctuations ◽  
Testosterone Metabolites ◽  
Sampling Procedures ◽  
Invasive Techniques ◽  
Fecal Testosterone

Testosterone is the main reproductive hormone in male vertebrates and conventional methods to measure testosterone rely on invasive blood sampling procedures. Here, we aimed to establish a non-invasive alternative by assessing testosterone metabolites (TMs) in fecal and urinary samples in mice. We performed a radiometabolism study to determine the effects of daytime and sex on the metabolism and excretion pattern of radiolabeled TMs. We performed physiological and biological validations of the applied EIA to measure TMs and assessed diurnal fluctuations in TM excretions in male and female mice and across strains. We found that males excreted significantly more radiolabeled TMs via the feces (59%) compared to females (49.5%). TM excretion patterns differed significantly between urinary and fecal samples and were affected by the daytime of ³H-testosterone injection. Overall, TM excretion occurred faster in urinary than fecal samples. Peak excretion of fecal TMs occurred after 8 h when animals received the 3H-testosterone in the morning, or after 4 h when they received the 3H-testosterone injection in the evening. Daytime had no effect on the formed TMs; however, males and females formed different types of TMs. As expected, males showed higher fecal TM levels than females. Males also showed diurnal fluctuations in their TM levels but we found no differences in the TM levels of C57BL/6J and B6D2F1 hybrid males. Finally, we successfully validated our applied EIA (measuring 17β-hydroxyandrostane) by showing that hCG (human chorionic gonadotropin) administration increased TM levels, whereas castration reduced them. In conclusion, our EIA proved suitable for measuring fecal TMs in mice. Our non-invasive method to assess fecal TMs can be widely used in various research disciplines like animal behavior, reproduction, animal welfare, ecology, conservation, and biomedicine.

Malfunctional Reorganization in the Developing Limbo-Prefrontal System in Animals: Implications for Human Psychoses?

2001 ◽  
Vol 12 (1) ◽  
pp. 8-14
Author(s):  
Gertraud Teuchert-Noodt ◽  
Ralf R. Dawirs
Keyword(s):  
Prefrontal Cortex ◽  
Mental Disorders ◽  
Dentate Gyrus ◽  
Cognitive Functions ◽  
Experimental Approach ◽  
Regulatory Mechanisms ◽  
Hippocampal Dentate Gyrus ◽  
Non Invasive ◽  
Invasive Method ◽  
Activity Dependent

Abstract: Neuroplasticity research in connection with mental disorders has recently bridged the gap between basic neurobiology and applied neuropsychology. A non-invasive method in the gerbil (Meriones unguiculus) - the restricted versus enriched breading and the systemically applied single methamphetamine dose - offers an experimental approach to investigate psychoses. Acts of intervening affirm an activity dependent malfunctional reorganization in the prefrontal cortex and in the hippocampal dentate gyrus and reveal the dopamine position as being critical for the disruption of interactions between the areas concerned. From the extent of plasticity effects the probability and risk of psycho-cognitive development may be derived. Advance may be expected from insights into regulatory mechanisms of neurogenesis in the hippocampal dentate gyrus which is obviously to meet the necessary requirements to promote psycho-cognitive functions/malfunctions via the limbo-prefrontal circuit.

scholarly journals CT perfusion in peripheral arterial disease—hemodynamic differences before and after revascularisation

2021 ◽  
Author(s):  
Patrick Veit-Haibach ◽  
Martin W. Huellner ◽  
Martin Banyai ◽  
Sebastian Mafeld ◽  
Johannes Heverhagen ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Ct Perfusion ◽  
Arterial Disease ◽  
Lower Leg ◽  
Therapy Control ◽  
Non Invasive ◽  
Before And After ◽  
Invasive Method ◽  
The Mean ◽  
Peripheral Arterial

Abstract Objectives The purpose of this study was the assessment of volumetric CT perfusion (CTP) of the lower leg musculature in patients with symptomatic peripheral arterial disease (PAD) before and after interventional revascularisation. Methods Twenty-nine consecutive patients with symptomatic PAD of the lower extremities requiring interventional revascularisation were assessed prospectively. All patients underwent a CTP scan of the lower leg, and hemodynamic and angiographic assessment, before and after intervention. Ankle-brachial pressure index (ABI) was determined. CTP parameters were calculated with a perfusion software, acting on a no outflow assumption. A sequential two-compartment model was used. Differences in CTP parameters were assessed with non-parametric tests. Results The cohort consisted of 24 subjects with an occlusion, and five with a high-grade stenosis. The mean blood flow before/after (BFpre and BFpost, respectively) was 7.42 ± 2.66 and 10.95 ± 6.64 ml/100 ml*min−1. The mean blood volume before/after (BVpre and BVpost, respectively) was 0.71 ± 0.35 and 1.25 ± 1.07 ml/100 ml. BFpost and BVpost were significantly higher than BFpre and BVpre in the treated limb (p = 0.003 and 0.02, respectively), but not in the untreated limb (p = 0.641 and 0.719, respectively). Conclusions CTP seems feasible for assessing hemodynamic differences in calf muscles before and after revascularisation in patients with symptomatic PAD. We could show that CTP parameters BF and BV are significantly increased after revascularisation of the symptomatic limb. In the future, this quantitative method might serve as a non-invasive method for surveillance and therapy control of patients with peripheral arterial disease. Key Points • CTP imaging of the lower limb in patients with symptomatic PAD seems feasible for assessing hemodynamic differences before and after revascularisation in PAD patients. • This quantitative method might serve as a non-invasive method, for surveillance and therapy control of patients with PAD.

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