scholarly journals Proteomics Approach for the Discovery of Rheumatoid Arthritis Biomarkers Using Mass Spectrometry

2019 ◽  
Vol 20 (18) ◽  
pp. 4368 ◽  
Author(s):  
Mun ◽  
Lee ◽  
Park ◽  
Kim ◽  
Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Mass Spectrometry ◽  
Vitamin D ◽  
Binding Protein ◽  
Joint Destruction ◽  
Protein Profiling ◽  
Serum Amyloid ◽  
Healthy Controls ◽  
Vitamin D Binding Protein ◽  
Proteomics Approach

Rheumatoid arthritis is an autoimmune disease that causes serious functional loss in patients. Early and accurate diagnosis of rheumatoid arthritis may attenuate its severity. Despite a diagnosis guideline in the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria for rheumatoid arthritis, the practical difficulties in its diagnosis highlight the need of developing new methods for diagnosing rheumatoid arthritis. The current study aimed to identify rheumatoid arthritis diagnostic biomarkers by using a proteomics approach. Serum protein profiling was conducted using mass spectrometry, and five distinguishable biomarkers were identified therefrom. In the validation study, the five biomarkers were quantitatively verified by multiple reaction monitoring (MRM) analysis. Two proteins, namely serum amyloid A4 and vitamin D binding protein, showed high performance in distinguishing patients with rheumatoid arthritis from healthy controls. Logistic analysis was conducted to evaluate how accurately the two biomarkers distinguish patients with rheumatoid arthritis from healthy controls. The classification accuracy was 86.0% and 81.4% in patients with rheumatoid arthritis and in healthy controls, respectively. Serum amyloid A4 and vitamin D binding protein could be potential biomarkers related to the inflammatory response and joint destruction that accompany rheumatoid arthritis.

scholarly journals Vitamin D in individuals before onset of rheumatoid arthritis - relation to vitamin D binding protein and its associated genetic variants

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Mikael Brink ◽  
Linda Johansson ◽  
Evelina Nygren ◽  
Lisbeth Ärlestig ◽  
Johan Hultdin ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Vitamin D ◽  
Genetic Variants ◽  
Binding Protein ◽  
Vitamin D Binding Protein

scholarly journals The relationship of 1,25-dihydroxyvitamin D and Vitamin D binding protein in periodontitis

2019 ◽  
Vol 35 (3) ◽  
Author(s):  
Sara Rafique ◽  
Mozaffer Rahim Hingorjo ◽  
Mahparah Mumtaz ◽  
Masood Anwar Qureshi
Keyword(s):  
Vitamin D ◽  
Binding Protein ◽  
Healthy Controls ◽  
Vitamin D Binding Protein ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D ◽  
Relationship Of ◽  
The Relationship

Objective: This study was conducted to explore the relationship between 1,25-dihydroxy vitamin D (1,25(OH)2D) and Vitamin-D binding protein (DBP) in patients with periodontitis and healthy controls. Methods: Seventy-five periodontitis cases were recruited from the dental OPD of Dow University of Health Sciences, Karachi. Diagnostic criteria of periodontitis were followed according to the probe pocket depth and clinical attachment loss. Seventy-five periodontal healthy controls were selected from the faculty and students of same university. Serum levels of 1,25(OH)2D and DBP were determined by ELISA. Results: Significantly low levels of 1,25(OH)2D and high levels of serum DBP were observed in periodontitis patients compared to healthy controls (p<0.05), with levels of DBP increasing significantly with the severity of periodontitis (p=0.005). Concentrations of DBP correlated positively with 1,25(OH)2D, especially in cases with periodontitis (r =0.780; p<0.001). Conclusion: Within the limits of the study, we conclude that low 1,25(OH)2D levels and high DBP levels are associated with periodontitis. doi: https://doi.org/10.12669/pjms.35.3.482 How to cite this:Rafique S, Hingorjo MR, Mumtaz M, Qureshi MA. The relationship of 1,25-dihydroxyvitamin D and Vitamin D binding protein in periodontitis. Pak J Med Sci. 2019;35(3):---------. doi: https://doi.org/10.12669/pjms.35.3.482 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Vitamin D–Binding Protein Concentrations Quantified by Mass Spectrometry

2015 ◽  
Vol 373 (15) ◽  
pp. 1480-1482 ◽  
Author(s):  
Andrew N. Hoofnagle ◽  
John H. Eckfeldt ◽  
Pamela L. Lutsey
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Binding Protein ◽  
Vitamin D Binding Protein

scholarly journals Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Sora Mun ◽  
Jiyeong Lee ◽  
Mi-Kyoung Lim ◽  
You-Rim Lee ◽  
Chunhwa Ihm ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Proteins ◽  
Drug Efficacy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Vitamin D Binding Protein ◽  
Serum Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Monitoring Drug ◽  
Proteomics Approach

Background. Rheumatoid arthritis (RA) is an autoimmune disease that starts with inflammation of the synovial membrane. Studies have been conducted to develop methods for efficient diagnosis of RA and to identify the mechanisms underlying RA development. Blood samples can be useful for detecting disturbance of homeostasis in patients with RA. Nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) is an efficient proteomics approach to analyze blood sample and quantify serum proteins. Methods. Serum samples of 18 healthy controls and 18 patients with RA were analyzed by LC-MS/MS. Selected candidate biomarkers were validated by enzyme-linked immunosorbent assay (ELISA) using sera from 43 healthy controls and 44 patients with RA. Results. Thirty-eight proteins were significantly differentially expressed by more than 2-fold in healthy controls and patients with RA. Based on a literature survey, we selected six candidate RA biomarkers. ELISA was used to evaluate whether these proteins effectively allow distinguishing patients with RA from healthy controls and monitoring drug efficacy. SAA4, gelsolin, and vitamin D-binding protein were validated as potential biomarkers of RA for screening and drug efficacy monitoring of RA. Conclusions. We identified a panel of three biomarkers for RA which has potential for application in RA diagnosis and drug efficacy monitoring. Further, our findings will aid in understanding the pathogenesis of RA.

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