scholarly journals Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes

2019 ◽  
Vol 20 (23) ◽  
pp. 5959 ◽  
Author(s):  
Silvia Carvajal ◽  
Meritxell Perramón ◽  
Gregori Casals ◽  
Denise Oró ◽  
Jordi Ribera ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Cerium Oxide ◽  
Antioxidant Properties ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxidant Injury ◽  
Ros Scavenging ◽  
Alcoholic Fatty Liver ◽  
The Impact

Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.

scholarly journals Cerium Oxide Nanoparticles: A Brief Review of Their Synthesis Methods and Biomedical Applications

Antioxidants ◽  
2018 ◽  
Vol 7 (8) ◽  
pp. 97 ◽  
Author(s):  
Atul Dhall ◽  
William Self
Keyword(s):  
Cerium Oxide ◽  
Biomedical Applications ◽  
Antioxidant Properties ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Synthesis Methods ◽  
Ros Scavenging ◽  
Corona Formation

Cerium oxide nanoparticles (CeNPs) exhibit antioxidant properties both in vitro and in vivo. This is due to the self-regeneration of their surface, which is based on redox-cycling between 3+ and 4+ states for cerium, in response to their immediate environment. Additionally, oxygen vacancies in the lattice structure allow for alternating between CeO2 and CeO2−x during redox reactions. Research to identify and characterize the biomedical applications of CeNPs has been heavily focused on investigating their use in treating diseases that are characterized by higher levels of reactive oxygen species (ROS). Although the bio-mimetic activities of CeNPs have been extensively studied in vitro, in vivo interactions and associated protein corona formation are not well understood. This review describes: (1) the methods of synthesis for CeNPs, including the recent green synthesis methods that offer enhanced biocompatibility and a need for establishing a reference CeNP material for consistency across studies; (2) their enzyme-mimetic activities, with a focus on their antioxidant activities; and, (3) recent experimental evidence that demonstrates their ROS scavenging abilities and their potential use in personalized medicine.

scholarly journals In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1565 ◽  
Author(s):  
Turin-Moleavin ◽  
Fifere ◽  
Lungoci ◽  
Rosca ◽  
Coroaba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Iron Oxide ◽  
Cerium Oxide ◽  
Chemical Activation ◽  
Radical Scavenging ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

Cerium oxide nanoparticles protects against acrylamide induced toxicity in HepG2 cells through modulation of oxidative stress

2018 ◽  
Vol 42 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Aala Azari ◽  
Mohammad Shokrzadeh ◽  
Ehsan Zamani ◽  
Nahid Amani ◽  
Fatemeh Shaki
Keyword(s):  
Oxidative Stress ◽  
Cerium Oxide ◽  
Hepg2 Cells ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Physicochemical characterization and antioxidant properties of cerium oxide nanoparticles

2018 ◽  
Vol 1115 ◽  
pp. 032094 ◽  
Author(s):  
R A Vazirov ◽  
S Y Sokovnin ◽  
V G Ilves ◽  
I N Bazhukova ◽  
N Pizurova ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Antioxidant Properties ◽  
Physicochemical Characterization ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Cerium oxide nanoparticles induce oxidative stress in the sediment-dwelling amphipodCorophium volutator

2015 ◽  
Vol 10 (4) ◽  
pp. 480-487 ◽  
Author(s):  
Yuktee Dogra ◽  
Kenton P. Arkill ◽  
Christine Elgy ◽  
Bjorn Stolpe ◽  
Jamie Lead ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cerium Oxide ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Different approaches to synthesise cerium oxide nanoparticles and their corresponding physical characteristics, ROS scavenging and anti-inflammatory capabilities

2021 ◽  
Author(s):  
Yuao Wu ◽  
Hang Thu Ta
Keyword(s):  
Cerium Oxide ◽  
Physical Characteristics ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Biological Applications ◽  
Ros Scavenging ◽  
Trivalent Cerium ◽  
Anti Inflammatory ◽  
Tetravalent Cerium

The biological applications of cerium oxide nanoparticles (nanoceria) have received extensive attention in recent decades. The coexistence of trivalent cerium and tetravalent cerium on the surface of nanoceria allows the...

scholarly journals Effect of Cerium Oxide Nanoparticles on Oxidative Stress Biomarkers in Rats’ Kidney, Lung, and Serum

2020 ◽  
Vol 24 (4) ◽  
pp. 251-256
Author(s):  
Adel Sepanjnia ◽  
Hassan Ghasemi ◽  
Roohollah Mohseni ◽  
Akram Ranjbar ◽  
Fatemeh Shabani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cerium Oxide ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers
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