scholarly journals Differential Gene Profiling of the Heartwood Formation Process in Taiwania cryptomerioides Hayata Xylem Tissues

2020 ◽  
Vol 21 (3) ◽  
pp. 960
Author(s):  
Yeh ◽  
Chu ◽  
Liu ◽  
Chen
Keyword(s):  
Formation Process ◽  
Lignin Biosynthesis ◽  
Gene Profiling ◽  
Limited Information ◽  
Differential Gene ◽  
Pinoresinol Reductase ◽  
Ray Parenchyma Cells ◽  
Taiwania Cryptomerioides ◽  
Cellular Components ◽  
Pinoresinol Lariciresinol Reductase

Taiwania (Taiwania cryptomerioides) is an important tree species in Taiwan because of the excellent properties of its wood and fascinating color qualities of its heartwood (HW), as well as the bioactive compounds therein. However, limited information is available as to the HW formation of this species. The objective of this research is to analyze the differentially expressed genes (DEGs) during the HW formation process from specific Taiwania xylem tissues, and to obtain genes that might be closely associated with this process. The results indicated that our analyses have captured DEGs representative to the HW formation process of Taiwania. DEGs related to the terpenoid biosynthesis pathway were all up-regulated in the transition zone (TZ) to support the biosynthesis and accumulation of terpenoids. Many DEGs related to lignin biosynthesis, and two DEGs related to pinoresinol reductase (PrR)/pinoresinol lariciresinol reductase (PLR), were up-regulated in TZ. These DEGs together are likely involved in providing the precursors for the subsequent lignan biosynthesis. Several transcription factor-, nuclease-, and protease-encoding DEGs were also highly expressed in TZ, and these DEGs might be involved in the regulation of secondary metabolite biosynthesis and the autolysis of the cellular components of ray parenchyma cells in TZ. These results provide further insights into the process of HW formation in Taiwania.

scholarly journals Differential gene profiling in acute lung injury identifies injury-specific gene expression*

2008 ◽  
Vol 36 (3) ◽  
pp. 855-865 ◽  
Author(s):  
Claudia C. dos Santos ◽  
Daisuke Okutani ◽  
Pingzhao Hu ◽  
Bing Han ◽  
Ettore Crimi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Gene Profiling ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Differential Gene

EDX microanalysis of cellular components in the pollen of an angiosperm, Plumbago zeylanica

1990 ◽  
Vol 48 (3) ◽  
pp. 804-805
Author(s):  
S. D. Russell
Keyword(s):  
Pollen Grains ◽  
Sperm Cells ◽  
Limited Information ◽  
Plumbago Zeylanica ◽  
X Ray ◽  
Distribution Of Elements ◽  
Specific Proteins ◽  
Quantum Detector ◽  
Cellular Components ◽  
Edx Microanalysis

Pollen grains in flowering plants recognize compatible stigmas and transmit the sperm cells that they contain to the egg. The pollen grains bear specific proteins and lipids on their outer walls, which are the principal sources of both pollen-stigma recognition and human allergic response; they also contain the organelles required to form the pollen tube and the two sperm cells that participate in double fertilization. Although limited information is available about x-ray microanalysis of pollen grains, no information is available about the cellular distribution of elements.The contents of pollen of Plumbago zeylanica L. were burst in double-distilled water onto formvar-coated Cu slot grids ( 1 × 2 mm) and air-dried at room temperature. Grids were then examined at 40 kV in a JEOL 2000 STEM (LaB6 e' source) equipped with a high-angle 10 mm2 Kevex Quantum detector and Delta analyzer. Spectra were collected for 100-500 livetime sec; digital x-ray maps were collected at resolutions of up to 128 × 256 (dwell time: 30 msec [10-15 collections], 15-30% deadtime at ca. 1,000 cps, adjusted using the free lens control).

scholarly journals Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

2009 ◽  
Vol 44 (3) ◽  
pp. 208-218 ◽  
Author(s):  
Albert-Adrien Ramelet ◽  
Nathalie Hirt-Burri ◽  
Wassim Raffoul ◽  
Corinne Scaletta ◽  
Dominique P. Pioletti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Therapy ◽  
Chronic Wound ◽  
Gene Profiling ◽  
Fetal Cell ◽  
Skin Cells ◽  
Differential Gene

scholarly journals Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues

2014 ◽  
Vol 31 (4) ◽  
pp. 1531-1538 ◽  
Author(s):  
AYA YAMAGISHI ◽  
SATOSHI MATSUMOTO ◽  
ATSUSHI WATANABE ◽  
YOSHIAKI MIZUGUCHI ◽  
KEISUKE HARA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Time Course ◽  
Gene Profiling ◽  
Bioinformatics Analyses ◽  
Differential Gene

scholarly journals Preferential Biological Processes in the Human Limbus by Differential Gene Profiling

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61833 ◽  
Author(s):  
Martin N. Nakatsu ◽  
Lily Vartanyan ◽  
Daniel M. Vu ◽  
Madelena Y. Ng ◽  
Xinmin Li ◽  
...  
Keyword(s):  
Gene Profiling ◽  
Biological Processes ◽  
Differential Gene

The gene expression and enzymatic activity of pinoresinol-lariciresinol reductase during wood formation in Taiwania cryptomerioides Hayata

Holzforschung ◽  
2019 ◽  
Vol 73 (2) ◽  
pp. 197-208 ◽  
Author(s):  
Nien-Ting Chiang ◽  
Li-Ting Ma ◽  
Yi-Ru Lee ◽  
Nai-Wen Tsao ◽  
Chih-Kai Yang ◽  
...  
Keyword(s):  
Wood Formation ◽  
Rna Seq ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reaction Products ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Lignan Biosynthesis ◽  
Polymerase Chain ◽  
Taiwania Cryptomerioides ◽  
Pinoresinol Lariciresinol Reductase

AbstractTaiwania (Taiwania cryptomerioidesHayata) is an indigenous conifer species of Taiwan. Various secondary metabolites of Taiwania with diverse bioactivities have been identified, and lignans are especially abundant in the heartwood (hW). In the present study, the wood of this species was separated to cambium (Cam), sapwood (sW), transition zone (TZ) and hW and their transcriptomes were sequenced. Three pinoresinol-lariciresinol reductases (PLRs; designatedTcPLR1,TcPLR2.2andTcPLR3), which are responsible for lignan biosynthesis, were cloned and their expressions in wood tissues were detected.TcPLRs had higher expression levels in Cam and sW in RNA-seq and reverse transcriptase-polymerase chain reaction (RT-PCR) results. Liquid chromatography-mass spectrometry (LC-MS) analysis of the reaction products of TcPLRs revealed that TcPLR1 can reduce (+)-pinoresinol to lariciresinol, and both TcPLR2.2 and TcPLR3 could reduce (+)-pinoresinol to lariciresinol and secoisolariciresinol.

Freeze-Fracture Replication in the Ultrastructure of Blue-Green Algae

1967 ◽  
Vol 25 ◽  
pp. 74-75
Author(s):  
L. V. Leak
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Green Algae ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Photosynthetic Membranes ◽  
Blue Green Algae ◽  
Cell Biol ◽  
Cellular Components

Electron microscopic observations of freeze-fracture replicas of Anabaena cells obtained by the procedures described by Bullivant and Ames (J. Cell Biol., 1966) indicate that the frozen cells are fractured in many different planes. This fracturing or cleaving along various planes allows one to gain a three dimensional relation of the cellular components as a result of such a manipulation. When replicas that are obtained by the freeze-fracture method are observed in the electron microscope, cross fractures of the cell wall and membranes that comprise the photosynthetic lamellae are apparent as demonstrated in Figures 1 & 2.A large portion of the Anabaena cell is composed of undulating layers of cytoplasm that are bounded by unit membranes that comprise the photosynthetic membranes. The adjoining layers of cytoplasm are closely apposed to each other to form the photosynthetic lamellae. Occassionally the adjacent layers of cytoplasm are separated by an interspace that may vary in widths of up to several 100 mu to form intralamellar vesicles.

Mitochondrial Reconstructions Based on Serial Thick Sections and HVEM

1975 ◽  
Vol 33 ◽  
pp. 286-287
Author(s):  
Jerome J. Paulin
Keyword(s):  
Imaging Techniques ◽  
Three Dimensional ◽  
Posterior Pole ◽  
Dimensional Structure ◽  
Stereo Imaging ◽  
Thin Sections ◽  
Anatomical Features ◽  
The Past ◽  
Cellular Components ◽  
Mitochondrial Reticulum

Within the past decade it has become apparent that HVEM offers the biologist a means to explore the three-dimensional structure of cells and/or organelles. Stereo-imaging of thick sections (e.g. 0.25-10 μm) not only reveals anatomical features of cellular components, but also reduces errors of interpretation associated with overlap of structures seen in thick sections. Concomitant with stereo-imaging techniques conventional serial Sectioning methods developed with thin sections have been adopted to serial thick sections (≥ 0.25 μm). Three-dimensional reconstructions of the chondriome of several species of trypanosomatid flagellates have been made from tracings of mitochondrial profiles on cellulose acetate sheets. The sheets are flooded with acetone, gluing them together, and the model sawed from the composite and redrawn.The extensive mitochondrial reticulum can be seen in consecutive thick sections of (0.25 μm thick) Crithidia fasciculata (Figs. 1-2). Profiles of the mitochondrion are distinguishable from the anterior apex of the cell (small arrow, Fig. 1) to the posterior pole (small arrow, Fig. 2).

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