A Novel Approach for Non-Invasive Lung Imaging and Targeting Lung Immune Cells

Despite developments in pulmonary radiotherapy, radiation-induced lung toxicity remains a problem. More sensitive lung imaging able to increase the accuracy of diagnosis and radiotherapy may help reduce this problem. Super-paramagnetic iron oxide nanoparticles are used in imaging, but without further modification can cause unwanted toxicity and inflammation. Complex carbohydrate and polymer-based coatings have been used, but simpler compounds may provide additional benefits. Herein, we designed and generated super-paramagnetic iron oxide nanoparticles coated with the neutral natural dietary amino acid glycine (GSPIONs), to support non-invasive lung imaging and determined particle biodistribution, as well as understanding the impact of the interaction of these nanoparticles with lung immune cells. These GSPIONs were characterized to be crystalline, colloidally stable, with a size of 12 ± 5 nm and a hydrodynamic diameter of 84.19 ± 18 nm. Carbon, Hydrogen, Nitrogen (CHN) elemental analysis estimated approximately 20.2 × 103 glycine molecules present per nanoparticle. We demonstrated that it is possible to determine the biodistribution of the GSPIONs in the lung using three-dimensional (3D) ultra-short echo time magnetic resonance imaging. The GSPIONs were found to be taken up selectively by alveolar macrophages and neutrophils in the lung. In addition, the GSPIONs did not cause changes to airway resistance or induce inflammatory cytokines. Alveolar macrophages and neutrophils are critical regulators of pulmonary inflammatory diseases, including allergies, infections, asthma and chronic obstructive pulmonary disease (COPD). Therefore, pulmonary Magnetic Resonance (MR) imaging and preferential targeting of these lung resident cells by our nanoparticles offer precise imaging tools, which can be utilized to develop precision targeted radiotherapy as well as diagnostic tools for lung cancer, thereby having the potential to reduce the pulmonary complications of radiation.

Download Full-text

Functionalized iron oxide nanoparticles for controlling the movement of immune cells

Nanoscale ◽

10.1039/c3nr04421a ◽

2015 ◽

Vol 7 (17) ◽

pp. 7780-7789 ◽

Cited By ~ 15

Author(s):

Ethan E. White ◽

Alex Pai ◽

Yiming Weng ◽

Anil K. Suresh ◽

Desiree Van Haute ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Immune Cells ◽

Oxide Nanoparticles ◽

Super Paramagnetic Iron Oxide ◽

Cpg Oligodeoxynucleotides

Coating super paramagnetic iron oxide nanoparticles with an immunostimulant, CpG oligodeoxynucleotides, dramatically increases their uptake by microglia cells. Once loaded with the nanoparticles, the microglia cells can be manipulated with magnets.

Download Full-text

Efficient Synthesis of Water-Soluble Magnetic Nanoparticles and Its Application in MRI in the Detection of Intracranial Aneurysm

Science of Advanced Materials ◽

10.1166/sam.2021.4108 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1699-1707

Author(s):

Boya Li ◽

Aihua Xiong ◽

Xiaotong Yang ◽

Qiong Yang ◽

Jing Liu

Keyword(s):

Iron Oxide ◽

Magnetic Nanoparticles ◽

Magnetic Resonance ◽

High Resolution ◽

Intracranial Aneurysm ◽

Iron Oxide Nanoparticles ◽

Water Soluble ◽

Oxide Nanoparticles ◽

Aneurysm Wall ◽

The Impact

Magnetic nanoparticles were used in medical images, which could further improve image clarity, while watersoluble nanoparticles put forward more new requirements for the biocompatibility of nanoparticles. This research adopted a simple and novel method to prepare water-soluble iron oxide nanoparticles. First, transmission electron microscope (TEM) was used to analyze the size distribution of the prepared product; X-ray diffraction (XRD) was used to test the crystal structure of the prepared sample; the fast Fourier transform (FFT) spectrum was introduced to analyze the structural properties of the nanoparticles; the nanoparticle aqueous solutions of different concentrations were designed, and the impact of water-soluble nanoparticles on magnetic resonance imaging (MRI) was examined with the nuclear magnetic resonance spectrometer. At the same time, the prepared water-soluble nanoparticle solution was used for high-resolution tumor wall imaging of patients with unruptured intracranial aneurysm (IA) to compare the imaging effect of the aneurysm wall before and after the introduction of nanoparticles. In the material characterization test of nanoparticles, the prepared samples did not have certain iron oxide characteristic peaks, which means the synthesized iron oxide nanoparticles did not have a fixed crystal morphology. The samples tested by energy dispersive spectrometer (EDS) also contained Fe, O, C and Na. The average particle size was 5.8 nm. It was found under high-resolution TEM that the particle mirror spacing was 0.48 nm, which was consistent with the 111-crystal plane of Fe3O4; The magnetic hysteresis loop test confirmed that when the concentration of nanoparticles increased, the solution would form a magnetic fluid. When the concentration of aqueous solution of nanoparticles increased, the corresponding MRI signal would be significantly enhanced. It was used in the MR scan of patients with unruptured IA. Nanoparticle solution could increase the visibility of the aneurysm, and the image quality of the aneurysm wall could be significantly enhanced.

Download Full-text

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

Journal of Drug Discovery and Therapeutics ◽

10.32553/jddt.v6i10.444 ◽

2018 ◽

Vol 6 (10) ◽

Author(s):

Hosam Zaghloul ◽

Doaa A. Shahin ◽

Ibrahim El- Dosoky ◽

Mahmoud E. El-awady ◽

Fardous F. El-Senduny ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Cellular Uptake ◽

Superparamagnetic Iron Oxide ◽

Cyclin B1 ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Mcf7 Cells ◽

M Phase ◽

The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Download Full-text