Sweet Cherry (Prunus avium L.) PaPIP1;4 Is a Functional Aquaporin Upregulated by Pre-Harvest Calcium Treatments that Prevent Cracking

The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl2, which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl2-treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H2O2 that is upregulated by the pre-harvest exogenous application of CaCl2 supplements.

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On the mechanisms of the sweet cherry (Prunus avium L.) fruit cracking: Swelling or shrinking?

Scientia Horticulturae ◽

10.1016/j.scienta.2015.01.002 ◽

2015 ◽

Vol 184 ◽

pp. 169-170 ◽

Cited By ~ 4

Author(s):

Kouman S. Koumanov

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Fruit Cracking ◽

Prunus Avium L

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Functional characterization of PaCYP724B1 involved in shoot branching in sweet cherry (Prunus avium L.)

Scientia Horticulturae ◽

10.1016/j.scienta.2019.108770 ◽

2019 ◽

Vol 258 ◽

pp. 108770

Author(s):

Qi Xiliang ◽

Liu Congli ◽

Song Lulu ◽

Qin Tengfei ◽

Li Ming

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Functional Characterization ◽

Shoot Branching ◽

Prunus Avium L

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Biochemical and phenotypic characterization of sweet cherry (Prunus avium L.) cultivars with induced surface pitting

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2021.111494 ◽

2021 ◽

Vol 175 ◽

pp. 111494

Author(s):

Excequel Ponce ◽

Blanca Alzola ◽

Natalia Cáceres ◽

Madeline Gas ◽

Catalina Ferreira ◽

...

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Phenotypic Characterization ◽

Prunus Avium L

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Multi-year analyses on three populations reveal the first stable QTLs for tolerance to rain-induced fruit cracking in sweet cherry (Prunus avium L.)

Horticulture Research ◽

10.1038/s41438-021-00571-6 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

José Quero-García ◽

Philippe Letourmy ◽

José Antonio Campoy ◽

Camille Branchereau ◽

Svetoslav Malchev ◽

...

Keyword(s):

Confidence Intervals ◽

Sweet Cherry ◽

Prunus Avium ◽

Linear Models ◽

Basic Research ◽

Fruit Weight ◽

Phenotypic Variance ◽

Fruit Cracking ◽

The Stability ◽

Sweet Cherry Cultivars

AbstractRain-induced fruit cracking is a major problem in sweet cherry cultivation. Basic research has been conducted to disentangle the physiological and mechanistic bases of this complex phenomenon, whereas genetic studies have lagged behind. The objective of this work was to disentangle the genetic determinism of rain-induced fruit cracking. We hypothesized that a large genetic variation would be revealed, by visual field observations conducted on mapping populations derived from well-contrasted cultivars for cracking tolerance. Three populations were evaluated over 7–8 years by estimating the proportion of cracked fruits for each genotype at maturity, at three different areas of the sweet cherry fruit: pistillar end, stem end, and fruit side. An original approach was adopted to integrate, within simple linear models, covariates potentially related to cracking, such as rainfall accumulation before harvest, fruit weight, and firmness. We found the first stable quantitative trait loci (QTLs) for cherry fruit cracking, explaining percentages of phenotypic variance above 20%, for each of these three types of cracking tolerance, in different linkage groups, confirming the high complexity of this trait. For these and other QTLs, further analyses suggested the existence of at least two-linked QTLs in each linkage group, some of which showed confidence intervals close to 5 cM. These promising results open the possibility of developing marker-assisted selection strategies to select cracking-tolerant sweet cherry cultivars. Further studies are needed to confirm the stability of the reported QTLs over different genetic backgrounds and environments and to narrow down the QTL confidence intervals, allowing the exploration of underlying candidate genes.

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The use of differential thermal analysis in determining the critical temperatures of sweet cherry (Prunus avium L.) flower buds at different stages of bud burst

International Journal of Biometeorology ◽

10.1007/s00484-021-02093-1 ◽

2021 ◽

Author(s):

Ozkan Kaya ◽

Cafer Kose ◽

Muge Sahin

Keyword(s):

Thermal Analysis ◽

Differential Thermal Analysis ◽

Sweet Cherry ◽

Prunus Avium ◽

Bud Burst ◽

Flower Buds ◽

Critical Temperatures ◽

Prunus Avium L

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Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map

Tree Genetics & Genomes ◽

10.1007/s11295-008-0161-1 ◽

2008 ◽

Vol 4 (4) ◽

pp. 897-910 ◽

Cited By ~ 59

Author(s):

James W. Olmstead ◽

Audrey M. Sebolt ◽

Antonio Cabrera ◽

Suneth S. Sooriyapathirana ◽

Sue Hammar ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Sweet Cherry ◽

Genetic Linkage Map ◽

Prunus Avium ◽

Synteny Analysis ◽

Reference Map ◽

Prunus Avium L

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PaCYP78A9, a Cytochrome P450, Regulates Fruit Size in Sweet Cherry (Prunus avium L.)

Frontiers in Plant Science ◽

10.3389/fpls.2017.02076 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 13

Author(s):

Xiliang Qi ◽

Congli Liu ◽

Lulu Song ◽

Yuhong Li ◽

Ming Li

Keyword(s):

Cytochrome P450 ◽

Sweet Cherry ◽

Prunus Avium ◽

Fruit Size ◽

Prunus Avium L

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Variability of Old Sweet Cherries Found in Slovak Regions and Their Preservation

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.1515/prolas-2017-0031 ◽

2017 ◽

Vol 71 (3) ◽

pp. 184-189

Author(s):

Michaela Benková ◽

Iveta Čičová ◽

Daniela Benedikova ◽

Lubomir Mendel ◽

Miroslav Glasa

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Fruit Size ◽

Morphological Characteristics ◽

Quality Parameters ◽

Fruit Weight ◽

Sweet Cherries ◽

Prunus Mahaleb ◽

Breeding Programmes ◽

Prunus Avium L

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.

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INSECT TRANSMISSION OF THE VIRUS CAUSING LITTLE CHERRY DISEASE

Canadian Journal of Plant Science ◽

10.4141/cjps60-103 ◽

1960 ◽

Vol 40 (4) ◽

pp. 707-712 ◽

Cited By ~ 6

Author(s):

W. H. A. Wilde

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Virus Disease ◽

Insect Transmission ◽

Prunus Avium L ◽

Cherry Trees

Little cherry virus disease of sweet cherry (Prunus avium L.) was transmitted under screenhouse conditions by 3 species of leafhoppers (Homoptera: Cicadellidae) out of 24 species tested. Macrosteles fascifrons (Stal), the 6-spotted leafhopper, transmitted the disease in seven tests; Scaphytopius acutus (Say), the sharp-nosed leafhopper, transmitted it once; and Psammotettix lividellus (Zett.) transmitted it once. The transmissions were made from diseased sweet cherry trees of the variety Lambert to indicators of the varieties Star or Sam. With the exception of 1 transmission, 2 to 4 years were necessary following inoculation for unmistakable expression of symptoms in the indicators. M. fascifrons was also implicated in 18 successful transmissions to mature sweet cherry trees grown in the open.

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