scholarly journals Transcriptional Responses of Resistant and Susceptible Wheat Exposed to Wheat Curl Mite

2021 ◽  
Vol 22 (5) ◽  
pp. 2703
Author(s):  
Mahnaz Kiani ◽  
Becky Bryan ◽  
Charles Rush ◽  
Adrianna Szczepaniec

(1) Background: The wheat curl mite (Aceria tosichella Keifer) is a key pest of wheat (Triticum aestivum L.) worldwide. While a number of wheat cultivars resistant to the mites have been employed to minimize the impact on the yield and quality of grain, little is known regarding the mechanisms underlying host plant resistance. Therefore, the goal of this study was to explore changes in transcriptome of resistant and susceptible wheat in order to quantify the molecular changes that drive host plant resistance. (2) Methods: Two varieties, wheat curl mite-susceptible (Karl 92) and wheat curl mite-resistant (TAM112) wheat, both at 2-week postemergence, were used in this study. Half of the plants were exposed to wheat curl mite herbivory and half remained mite-free and served as controls. Transcriptome changes were quantified using RNA-seq and compared among treatments to identify genes and pathways affected by herbivores. (3) Results: We identified a number of genes and pathways involved in plant defenses against pathogens, herbivores, and abiotic stress that were differentially expressed in the resistant wheat exposed to wheat curl mite herbivory but were unaffected in the susceptible wheat. (4) Conclusions: Our outcomes indicated that resistant wheat counteracts wheat curl mite exposure through effective induction of genes and pathways that enhance its defense responses.

2021 ◽  
Vol 42 (2) ◽  
pp. 326-331
Author(s):  
M. Singh ◽  
◽  
K.S. Sandhu ◽  

Aim: To determine the impact of soil and foliar application of ZnSO4.7H2O at late stages of wheat as heading initiation (5% ear formation), 100% heading (complete ear formation) and heading initiation and 100% heading along with recommended dose of fertilizer on growth, yield and quality of zero till wheat. Methodology: The field experiment was conducted on zero till wheat. The treatments consisted of control (no Zn), soil application of 12.5, 25, 37.5, 50 kg ha-1 ZnSO4.7H2O and foliar application of 0.5% Zn as one spray at heading initiation (5% ear formation), one spray at 100% heading (complete ear formation) and two sprays at heading initiation and 100% heading with recommended dose of fertilizer. These treatments were evaluated in RBD with three replications. Results: Soil application of 50, 37.5 and 25 kg Zn ha-1 with two foliar sprays of 0.5% at heading initiation (5% ear formation) and 100% heading (complete ear formation) stages gave significantly higher average grain and straw yield and Zn concentration in grain than other treatments, including control. Interpretation: Enhanced application of Zn as soil and foliar application ameliorates soil Zn deficiency and increases protein content in grains, which might influence the quality and yield of zero tilled wheat. Key words: Foliar spray, Grain yield, Wheat, Zinc


1988 ◽  
Vol 68 (2) ◽  
pp. 439-447 ◽  
Author(s):  
A. W. LENSSEN ◽  
E. L. SORENSEN ◽  
G. L. POSLER ◽  
L. H. HARBERS

Host-plant resistance in alfalfa (Medicago sativa L.) is insufficient for control of the alfalfa weevil (Hypera postica) or the potato leafhopper (Empoasca fabae), two of the most important insect pests of alfalfa. Some wild Medicago species, which have erect glandular hairs, possess adequate host-plant resistance for control of both pests. We established a field trial (Wymore silty clay loam) in 1985 to determine the effects of erect glandular hairs on forage quality of several perennial Medicago species. Glandular and eglandular (without erect glandular hairs) plant populations were selected from the diploids, M. prostrata Jacq. and M. glandulosa David, and the tetraploids, M. glutinosa Bieb., M. sativa × M. glutinosa, and M. sativa × M. prostrata. Eglandular M. sativa ’Riley’ and M. sativa subsp. caerulea (Less, ex Ledeb.) Schmalh. were included as controls. Foliar diseases and insects were controlled. Leaves and stems were separated for three harvests in 1985 and one in 1986. The presence of erect glandular hairs did not significantly affect concentrations of neutral or acid detergent fibers, hemicellulose, lignin, or cellulose of leaves or stems within the species or hybrids tested. Neutral and acid detergent fibers and cellulose concentrations were generally lower in stems and higher in leaves of diploids than in corresponding parts of the tetraploid alfalfa cultivar Riley.Key words: Alfalfa, Medicago sp., glandular hairs, forage quality


2021 ◽  
Vol 37 (5) ◽  
pp. 428-436
Author(s):  
Chutsuda Junsopa ◽  
Weerasak Saksirirat ◽  
Suwita Saepaisan ◽  
Patcharin Songsri ◽  
Thawan Kesmala ◽  
...  

Stem rot is a serious disease in Jerusalem artichoke (JA). To reduce the impact of this disease on yield and quality farmers often use fungicides, but this control method can be expensive and leave chemical residues. The objective of this study was to evaluate the efficacy of two biological control agents, Trichoderma harzianum T9 and Bacillus firmus BSR032 for control of Sclerotium rolfsii under field conditions. Four accessions of JA (HEL246, HEL65, JA47, and JA12) were treated or notreated with T. harzianum T9 and B. firmus BSR032 in a 4 × 2 × 2 factorial experiment in two fields (environments), one unfertilized and one fertilized. Plants were inoculated with S. rolfsii and disease was evaluated at 3-day intervals for 46 days. T. harzianum T9 and B. firmus BSR032 reduced disease incidence by 48% and 49%, respectively, whereas T. harzianum T9 + B. firmus BSR032 reduced disease incidence by 37%. The efficacy of T. harzianum T9 and B. firmus BSR032 for control of S. rolfsii was dependent on environments and genotypes. The expression of host plant resistance also depended on the environment. However, HEL246 showed consistently low disease incidence and severity index in both environments (fertilized and unfertilized). Individually, T. harzianum T9, B. firmus BSR032, or host plant resistance control stem rot caused by S. rolfsii in JA. However, no combination of these treatments provided more effective control than each alone.


2020 ◽  
Vol 113 (6) ◽  
pp. 2972-2978 ◽  
Author(s):  
Ashley D Yates-Stewart ◽  
Adrian Pekarcik ◽  
Andy Michel ◽  
Joshua J Blakeslee

Abstract Host-plant resistance (HPR) is an important tool for pest management, affording both economic and environmental benefits. The mechanisms of aphid resistance in soybean are not well understood, but likely involve the induction of the jasmonic acid (JA) pathway, and possibly other phytohormone signals involved in plant defense responses. Despite the efficacy of aphid resistance in soybean, virulent aphids have overcome this resistance through mostly unknown mechanisms. Here, we have used metabolomic tools to define the role of plant phytohormones, especially the JA pathway, in regulating interactions between aphid-resistant soybean and virulent aphids. We hypothesized that virulent aphids avoid or suppress the JA pathway to overcome aphid resistance. Our results suggested that aphid-resistant soybean increased accumulation of JA-isoleucine (JA-Ile) only when infested with avirulent aphids; virulent aphids did not cause induction of JA-Ile. Further, applying JA-Ile to aphid-resistant soybean reduced subsequent virulent aphid populations. The concentrations of other phytohormones remained unchanged due to aphid feeding, highlighting the importance of JA-Ile in this interaction. These results increase our knowledge of soybean resistance mechanisms against soybean aphids and contribute to our understanding of aphid virulence mechanisms, which will in turn promote the durability of HPR.


2018 ◽  
Author(s):  
C. Coy ◽  
A.V. Shuravilin ◽  
O.A. Zakharova

Приведены результаты исследований по изучению влияния промышленной технологии возделывания картофеля на развитие, урожайность и качество продукции. Выявлена положительная реакция растений на подкормку K2SO4 в период посадки. Корреляционно-регрессионный анализ урожайности и качества клубней выявил высокую степень достоверности результатов опыта. Содержание нитратов и тяжелых металлов в клубнях было ниже допустимых величин.The results of studies on the impact of industrial technology of potato cultivation on growth, yield and quality of products. There was a positive response of plants to fertilizer K2SO4 in the period of planting. Correlation and regression analysis of yield and quality of tubers revealed a high degree of reliability of the results of experience. The contents of nitrates and heavy metals in tubers was below the permissible values.


2020 ◽  
Vol 7 (04) ◽  
Author(s):  
A B Priyanshu ◽  
M K Singh ◽  
Mukesh Kumar ◽  
Vipin Kumar ◽  
Sunil Malik ◽  
...  

An experiment was conducted at Horticultural Research Centre, SVP University of Agriculture and Technology, Meerut (UP) during Rabi season of 2018-19 to assess the impact of different INM doses on yield and quality parameters of garlic. A total of ten treatments consisting of combinations of inorganic fertilizers, organic fertilizers and bio-fertilizers like T1- (Control), T2RDF (100:50:50 kg NPK ha-1), T3-RDF + 20 kg sulphur + FYM 20 ton ha-1, T4- RDF + 20 kg sulphur + VC 4 ton ha-1, T5- 75% RDF + 40 kg sulphur + 5 ton FYM ha-1+ PSB 5 kg ha-1, T6-75% RDF + 40 kg sulphur + 2 ton VC + Azotobacter 5 kg ha-1, T7- 75% RDF + 40 kg sulphur + FYM 3 ton + VC 1 ton+ PSB 5 kg + Azotobacter 5kg ha-1, T8- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg ha-1, T9- 50% RDF + 40 kg sulphur + FYM5 ton + VC 2 ton+ Azotobacter 5 kg ha-1and T10- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg + Azotobacter 5 kg ha-1 were used in Randomized Block Design and replicated thrice. Out of these an application of T7 (75% RDF + 40 kg sulphur + FYM3 ton + VC 1 ton ha-1 + PSB 5 kg + Azotobacter 5 kg ha-1) was found to be significantly superior in term of yield and attributing parameters of garlic.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 642
Author(s):  
Yuliia Kochiieru ◽  
Audronė Mankevičienė ◽  
Jurgita Cesevičienė ◽  
Roma Semaškienė ◽  
Jūratė Ramanauskienė ◽  
...  

In this work, we studied the impact of harvesting time on Fusarium mycotoxin occurrence in spring wheat and the effect of mycotoxin contamination on the quality of these grains. The spring wheat grains (Triticum aestivum L.) were collected in 2016–2018 when the crop had reached full maturity, 10 ± 2 days and 17 ± 3 days after full maturity. The grain samples were analyzed for Fusarium infection and co-contamination with mycotoxins deoxynivalenol (DON), zearalenone (ZEA), and T-2 toxin (T-2), as well as the quality of the wheat grains (mass per hectolitre, contents of protein, starch, ash and fat, particle size index (PSI), falling number, sedimentation, wet gluten content, and gluten index). The occurrence of Fusarium spp. fungi and the mycotoxins produced by them in the grains was mostly influenced by the harvesting time and meteorological conditions. The correlations between Fusarium species and the mycotoxins produced by them in the grains of spring wheat showed F. graminearum to be a dominant species, and as a result, higher concentrations of DON and ZEA were determined. The co-occurrence of all the three mycotoxins analyzed (deoxynivalenol, zearalenone, and T-2 toxin) was identified in wheat. In rainy years, a delay in harvesting resulted in diminished grain quality of spring wheat, as indicated by grain mass per hectolitre and falling number. Negative correlations were found in highly contaminated grains between mycotoxins (DON, ZEA, and T-2) and falling number and grain mass per hectolitre values.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1384
Author(s):  
Dinar S. C. Wahyuni ◽  
Young Hae Choi ◽  
Kirsten A. Leiss ◽  
Peter G. L. Klinkhamer

Understanding the mechanisms involved in host plant resistance opens the way for improved resistance breeding programs by using the traits involved as markers. Pest management is a major problem in cultivation of ornamentals. Gladiolus (Gladiolus hybridus L.) is an economically important ornamental in the Netherlands. Gladiolus is especially sensitive to attack by western flower thrips (Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae)). The objective of this study was, therefore, to investigate morphological and chemical markers for resistance breeding to western flower thrips in Gladiolus varieties. We measured thrips damage of 14 Gladiolus varieties in a whole-plant thrips bioassay and related this to morphological traits with a focus on papillae density. Moreover, we studied chemical host plant resistance to using an eco-metabolomic approach comparing the 1H NMR profiles of thrips resistant and susceptible varieties representing a broad range of papillae densities. Thrips damage varied strongly among varieties: the most susceptible variety showed 130 times more damage than the most resistant one. Varieties with low thrips damage had shorter mesophylls and epidermal cells, as well as a higher density of epicuticular papillae. All three traits related to thrips damage were highly correlated with each other. We observed a number of metabolites related to resistance against thrips: two unidentified triterpenoid saponins and the amino acids alanine and threonine. All these compounds were highly correlated amongst each other as well as to the density of papillae. These correlations suggest that papillae are involved in resistance to thrips by producing and/or storing compounds causing thrips resistance. Although it is not possible to distinguish the individual effects of morphological and chemical traits statistically, our results show that papillae density is an easy marker in Gladiolus-breeding programs targeted at increased resistance to thrips.


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