scholarly journals Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

2021 ◽  
Vol 22 (9) ◽  
pp. 4491
Author(s):  
Sunil S. Gangurde ◽  
Spurthi N. Nayak ◽  
Pushpesh Joshi ◽  
Shilp Purohit ◽  
Hari K. Sudini ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Genes ◽  
Transcriptome Analysis ◽  
Leaf Spot ◽  
Yield Loss ◽  
Disease Resistance Genes ◽  
Resistance Locus ◽  
Comparative Transcriptome ◽  
Late Leaf Spot ◽  
Comparative Transcriptome Analysis

Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.

scholarly journals Transcriptome Analysis Reveals the Inducing Effect of Bacillus siamensis on Disease Resistance in Postharvest Mango Fruit

Foods ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 107
Author(s):  
Zecheng Jiang ◽  
Rui Li ◽  
Yue Tang ◽  
Ziyu Cheng ◽  
Minjie Qian ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Transcriptome Analysis ◽  
Postharvest Disease ◽  
Comparative Transcriptome ◽  
Postharvest Diseases ◽  
Mango Fruit ◽  
Comparative Transcriptome Analysis ◽  
Fruit Samples ◽  
Transcriptional Regulatory Mechanisms

Postharvest anthracnose, caused by the fungus Colletotrichum gloeosporioides, is one of the most important postharvest diseases of mangoes worldwide. Bacillus siamensis (B. siamensis), as a biocontrol bacteria, has significant effects on inhibiting disease and improving the quality of fruits and vegetables. In this study, pre-storage application of B. siamensis significantly induced disease resistance and decreased disease index (DI) of stored mango fruit. To investigate the induction mechanisms of B. siamensis, comparative transcriptome analysis of mango fruit samples during the storage were established. In total, 234,808 unique transcripts were assembled and 56,704 differentially expressed genes (DEGs) were identified by comparative transcriptome analysis. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs showed that most of the DEGs involved in plant-pathogen interaction, plant hormone signal transduction, and biosynthesis of resistant substances were enriched. Fourteen DEGs related to disease-resistance were validated by qRT-PCR, which well corresponded to the FPKM value obtained from the transcriptome data. These results indicate that B. siamensis treatment may act to induce disease resistance of mango fruit by affecting multiple pathways. These findings not only reveal the transcriptional regulatory mechanisms that govern postharvest disease, but also develop a biological strategy to maintain quality of post-harvest mango fruit.

scholarly journals New Andean source of resistance to anthracnose and angular leaf spot: Fine-mapping of disease-resistance genes in California Dark Red Kidney common bean cultivar

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0235215 ◽  
Author(s):  
M. C. Gonçalves-Vidigal ◽  
T. A. S. Gilio ◽  
G. Valentini ◽  
M. Vaz-Bisneta ◽  
P. S. Vidigal Filho ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Common Bean ◽  
Resistance Genes ◽  
Fine Mapping ◽  
Leaf Spot ◽  
Disease Resistance Genes ◽  
Angular Leaf Spot ◽  
Bean Cultivar ◽  
Common Bean Cultivar

Genome-Wide Analysis of NBS-Encoding Disease Resistance Genes in Maize Inbred Line B73

2009 ◽  
Vol 35 (3) ◽  
pp. 566-570 ◽  
Author(s):  
Jie-Ming WANG ◽  
Hai-Yang JIANG ◽  
Yang ZHAO ◽  
Yan XIANG ◽  
Su-Wen ZHU ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Inbred Line ◽  
Resistance Genes ◽  
Disease Resistance Genes ◽  
Maize Inbred Line ◽  
Genome Wide Analysis ◽  
Genome Wide

Comparative Mapping of Three Bacterial, Three Fungal, and One Virus Disease-resistance Genes in Common Bean

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 547a-547
Author(s):  
Geunhwa Jung ◽  
James Nienhuis ◽  
Dermot P. Coyne ◽  
H.M. Ariyarathne
Keyword(s):  
Disease Resistance ◽  
Common Bean ◽  
Pseudomonas Syringae ◽  
Resistance Genes ◽  
Fungal Pathogens ◽  
Xanthomonas Campestris ◽  
Virus Disease ◽  
Disease Resistance Genes ◽  
Brown Spot ◽  
Viral Pathogen

Common bacterial blight (CBB), bacterial brown spot (BBS), and halo blight (HB), incited by the bacterial pathogens Xanthomonas campestris pv. phaseoli (Smith) Dye, Pseodomonas syringae pv. syringa, and Pseudomonas syringae pv. phaseolicola, respectively are important diseases of common bean. In addition three fungal pathogens, web blight (WB) Thanatephorus cucumeris, rust Uromyces appendiculatus, and white mold (WM) Sclerotinia sclerotiorum, are also destructive diseases attacking common bean. Bean common mosaic virus is also one of most major virus disease. Resistance genes (QTLs and major genes) to three bacterial (CBB, BBS, and HB), three fungal (WB, rust, and WM), and one viral pathogen (BCMV) were previously mapped in two common bean populations (BAC 6 × HT 7719 and Belneb RR-1 × A55). The objective of this research was to use an integrated RAPD map of the two populations to compare the positions and effect of resistance QTL in common bean. Results indicate that two chromosomal regions associated with QTL for CBB resistance mapped in both populations. The same chromosomal regions associated with QTL for disease resistance to different pathogens or same pathogens were detected in the integrated population.

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