scholarly journals Transcriptome Analysis Reveals the Inducing Effect of Bacillus siamensis on Disease Resistance in Postharvest Mango Fruit

Foods ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 107
Author(s):  
Zecheng Jiang ◽  
Rui Li ◽  
Yue Tang ◽  
Ziyu Cheng ◽  
Minjie Qian ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Transcriptome Analysis ◽  
Postharvest Disease ◽  
Comparative Transcriptome ◽  
Postharvest Diseases ◽  
Mango Fruit ◽  
Comparative Transcriptome Analysis ◽  
Fruit Samples ◽  
Transcriptional Regulatory Mechanisms

Postharvest anthracnose, caused by the fungus Colletotrichum gloeosporioides, is one of the most important postharvest diseases of mangoes worldwide. Bacillus siamensis (B. siamensis), as a biocontrol bacteria, has significant effects on inhibiting disease and improving the quality of fruits and vegetables. In this study, pre-storage application of B. siamensis significantly induced disease resistance and decreased disease index (DI) of stored mango fruit. To investigate the induction mechanisms of B. siamensis, comparative transcriptome analysis of mango fruit samples during the storage were established. In total, 234,808 unique transcripts were assembled and 56,704 differentially expressed genes (DEGs) were identified by comparative transcriptome analysis. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs showed that most of the DEGs involved in plant-pathogen interaction, plant hormone signal transduction, and biosynthesis of resistant substances were enriched. Fourteen DEGs related to disease-resistance were validated by qRT-PCR, which well corresponded to the FPKM value obtained from the transcriptome data. These results indicate that B. siamensis treatment may act to induce disease resistance of mango fruit by affecting multiple pathways. These findings not only reveal the transcriptional regulatory mechanisms that govern postharvest disease, but also develop a biological strategy to maintain quality of post-harvest mango fruit.

scholarly journals Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

2021 ◽  
Vol 22 (9) ◽  
pp. 4491
Author(s):  
Sunil S. Gangurde ◽  
Spurthi N. Nayak ◽  
Pushpesh Joshi ◽  
Shilp Purohit ◽  
Hari K. Sudini ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Genes ◽  
Transcriptome Analysis ◽  
Leaf Spot ◽  
Yield Loss ◽  
Disease Resistance Genes ◽  
Resistance Locus ◽  
Comparative Transcriptome ◽  
Late Leaf Spot ◽  
Comparative Transcriptome Analysis

Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.

scholarly journals Pengaruh Beberapa Khamir Antagonis terhadap Penyakit Antraknosa dan Umur Simpan pada Buah Mangga

2019 ◽  
Vol 14 (5) ◽  
pp. 153
Author(s):  
Riana Jumawati ◽  
Roedhy Poerwanto ◽  
Suryo Wiyono ◽  
Ketty Suketi
Keyword(s):  
Shelf Life ◽  
Room Temperature ◽  
Control Measure ◽  
Postharvest Disease ◽  
Postharvest Diseases ◽  
Mango Fruit ◽  
Anthracnose Disease ◽  
Comparative Treatment ◽  
Two Temperature

Effect of Various Yeasts Antagonists on Anthracnose Disease and the Shelf Life of MangoPostharvest decay on mango may occur due to physiological damage and/or postharvest disease especially anthracnose. This postharvest decay may decrease the quality of mango fruit. The use of antagonists yeast as biocontrol agent is an alternative control measure for postharvest diseases of fruits. This study aimed to screen yeast species as biological control agents and in the same time delaying the maturity of fruits to extend its shelf life.  Six isolates of yeasts were evaluated, namely Cryptococcus albidus, Cryptococcus terreus, Aureobasidium pullulans, Rhodotorula minuta, Candida tropicalis, Pseoudozyma hubeiensis); fungicides treatment using azoksistrobin was applied for comparative treatment. The study was conducted at two temperature conditions, namely room temperature and 15 ° C. It was evidenced that C. albidus, A. pullulans, and C. tropicalis were effective to control anthracnose disease on mango under both temperatures.  Furthermore, C. tropicalis and A. pullulans were able to extend manggo shelf life for 21 days at room temperature and for 54 days at 15 °C, respectively.

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