Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period—An In Vitro Study

The electromagnetic field (EMF) affects the physiological processes in mammals, but the molecular background of the observed alterations remains not well established. In this study was tested the effect of short duration (2 h) of the EMF treatment (50 Hz, 8 mT) on global transcriptomic alterations in the myometrium of pigs during the peri-implantation period using next-generation sequencing. As a result, the EMF treatment affected the expression of 215 transcript active regions (TARs), and among them, the assigned gene protein-coding biotype possessed 90 ones (differentially expressed genes, DEGs), categorized mostly to gene ontology terms connected with defense and immune responses, and secretion and export. Evaluated DEGs enrich the KEGG TNF signaling pathway, and regulation of IFNA signaling and interferon-alpha/beta signaling REACTOME pathways. There were evaluated 12 differentially expressed long non-coding RNAs (DE-lnc-RNAs) and 182 predicted single nucleotide variants (SNVs) substitutions within RNA editing sites. In conclusion, the EMF treatment in the myometrium collected during the peri-implantation period affects the expression of genes involved in defense and immune responses. The study also gives new insight into the mechanisms of the EMF action in the regulation of the transcriptomic profile through lnc-RNAs and SNVs.

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Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)

Cells ◽

10.3390/cells10040921 ◽

2021 ◽

Vol 10 (4) ◽

pp. 921

Author(s):

Aleksandra Lipka ◽

Jan Pawel Jastrzebski ◽

Lukasz Paukszto ◽

Karol Gustaw Makowczenko ◽

Elzbieta Lopienska-Biernat ◽

...

Keyword(s):

Fetal Growth ◽

Fetal Growth Restriction ◽

Target Genes ◽

Active Regions ◽

Bioinformatic Analysis ◽

Growth Restriction ◽

Differentially Expressed ◽

Circular Rnas ◽

Differential Analysis ◽

Protein Coding

Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate non-coding RNAs (lncRNAs) in the placentas of male and female fetuses affected by FGR. RNA-Seq data were analyzed to detect lncRNAs, their potential target genes and circular RNAs (circRNAs); a differential analysis was also performed. The multilevel bioinformatic analysis enabled the detection of 23,137 placental lncRNAs and 4263 of them were classified as novel. In FGR-affected female fetuses’ placentas (ff-FGR), among 19 transcriptionally active regions (TARs), five differentially expressed lncRNAs (DELs) and 12 differentially expressed protein-coding genes (DEGs) were identified. Within 232 differentially expressed TARs identified in male fetuses (mf-FGR), 33 encompassed novel and 176 known lncRNAs, and 52 DEGs were upregulated, while 180 revealed decreased expression. In ff-FGR ACTA2-AS1, lncRNA expression was significantly correlated with five DEGs, and in mf-FGR, 25 TARs were associated with DELs correlated with 157 unique DEGs. Backsplicing circRNA processes were detected in the range of H19 lncRNA, in both ff- and mf-FGR placentas. The performed global lncRNAs characteristics in terms of fetal sex showed dysregulation of DELs, DEGs and circRNAs that may affect fetus growth and pregnancy outcomes. In female placentas, DELs and DEGs were associated mainly with the vasculature, while in male placentas, disturbed expression predominantly affected immune processes.

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Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17 β (E 2 ) in uterine tissues: An in vitro study

Theriogenology ◽

10.1016/j.theriogenology.2017.12.041 ◽

2018 ◽

Vol 110 ◽

pp. 86-95 ◽

Cited By ~ 3

Author(s):

Anna Koziorowska ◽

Ewa Monika Waszkiewicz ◽

Maria Romerowicz-Misielak ◽

Kamila Zglejc-Waszak ◽

Anita Franczak

Keyword(s):

Electromagnetic Field ◽

Low Frequency ◽

In Vitro Study ◽

Vitro Study ◽

Extremely Low Frequency

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Long Non-coding RNAs RN7SK and GAS5 Regulate Macrophage Polarization and Innate Immune Responses

Frontiers in Immunology ◽

10.3389/fimmu.2020.604981 ◽

2020 ◽

Vol 11 ◽

Author(s):

Imran Ahmad ◽

Araceli Valverde ◽

Raza Ali Naqvi ◽

Afsar R. Naqvi

Keyword(s):

Immune Responses ◽

Epigenetic Regulation ◽

Innate Immune ◽

Differentially Expressed ◽

Immune Functions ◽

Innate Immune Responses ◽

Antigen Uptake ◽

Non Coding Rnas

Macrophages (Mφ) are immune cells that exhibit remarkable functional plasticity. Identification of novel endogenous factors that can regulate plasticity and innate immune functions of Mφ will unravel new strategies to curb immune-related diseases. Long non-coding RNAs (lncRNAs) are a class of endogenous, non-protein coding, regulatory RNAs that are increasingly being associated with various cellular functions and diseases. Despite their ubiquity and abundance, lncRNA-mediated epigenetic regulation of Mφ polarization and innate immune functions is poorly studied. This study elucidates the regulatory role of lncRNAs in monocyte to Mφ differentiation, M1/M2 dichotomy and innate immune responses. Expression profiling of eighty-eight lncRNAs in monocytes and in vitro differentiated M2 Mφ identified seventeen differentially expressed lncRNAs. Based on fold-change and significance, we selected four differentially expressed lncRNAs viz., RN7SK, GAS5, IPW, and ZFAS1 to evaluate their functional impact. LncRNA knockdown was performed on day 3 M2 Mφ and the impact on polarization was assessed on day 7 by surface marker analysis. Knockdown of RN7SK and GAS5 showed downregulation of M2 surface markers (CD163, CD206, or Dectin) and concomitant increase in M1 markers (MHC II or CD23). RN7SK or GAS5 knockdown showed no significant impact on CD163, CD206, or CD23 transcripts. M1/M2 markers were not impacted by IPW or ZFAS1 knockdown. Functional regulation of antigen uptake/processing and phagocytosis, two central innate immune pathways, by candidate lncRNA was assessed in M1/M2 Mφ. Compared to scramble, enhanced antigen uptake and processing were observed in both M1/M2 Mφ transfected with siRNA targeting GAS5 and RN7SK but not IPW and ZFAS1. In addition, knockdown of RN7SK significantly augmented uptake of labelled E. coli in vitro by M1/M2 Mφ, while no significant difference was in GAS5 silencing cells. Together, our results highlight the instrumental role of lncRNA (RN7SK and GAS5)-mediated epigenetic regulation of macrophage differentiation, polarization, and innate immune functions.

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lncRNAs Are Involved in Sevoflurane Anesthesia-Related Brain Function Modulation through Affecting Mitochondrial Function and Aging Process

BioMed Research International ◽

10.1155/2020/8841511 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Yinyin Qu ◽

Hongyi Li ◽

Chengmei Shi ◽

Min Qian ◽

Ning Yang ◽

...

Keyword(s):

Brain Function ◽

Brain Dysfunction ◽

Differentially Expressed ◽

Sevoflurane Anesthesia ◽

Protein Coding ◽

In Vivo Experiments ◽

Coexpression Networks ◽

And Oxidative Stress

Long noncoding RNAs (lncRNAs) play important roles in brain function modulation and neurodegenerative diseases. However, whether lncRNA regulations are involved in the mechanisms of perioperative neurocognitive disorders, especially in anesthesia-related brain dysfunction, remain unknown. Therefore, we explored the expression and regulation pattern profiles of lncRNAs in the hippocampus of aged rats after sevoflurane anesthesia. Three lncRNAs and 772 protein-coding genes were identified by microarray analysis and evidenced by in vitro and in vivo experiments as differentially expressed. Functional annotation and differentially expressed- (DE-) lncRNA-mRNA coexpression networks reveal that DE-lncRNAs are associated with mitochondrial dysfunction and oxidative stress, aging-related metabolism alterations, DNA damage, and apoptosis, as well as neurodegenerative features during sevoflurane anesthesia. These results suggest that lncRNAs play roles in general anesthesia-related brain function modulation during the perioperative context and provide insights into the lncRNA-related modulation mechanisms and targets.

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IN-VITRO STUDY OF IMMUNE RESPONSES IN LOIN PAIN/HAEMATURIA SYNDROME

The Lancet ◽

10.1016/s0140-6736(89)90154-2 ◽

1989 ◽

Vol 333 (8652) ◽

pp. 1451

Author(s):

StephenM.W. Hutchison ◽

Gordon Harkiss

Keyword(s):

Immune Responses ◽

In Vitro Study ◽

Vitro Study ◽

Loin Pain ◽

Loin Pain Haematuria Syndrome

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Barberry's (Berberis integerrima) ingredients suppress T-cell response and shift immune responses toward Th2: an in vitro study

Future Science OA ◽

10.4155/fso.15.49 ◽

2015 ◽

Vol 1 (4) ◽

Cited By ~ 1

Author(s):

Shirin Fateh ◽

Shaghayegh Pishkhan Dibazar ◽

Saeed Daneshmandi

Keyword(s):

T Cell ◽

Immune Responses ◽

Cell Response ◽

In Vitro Study ◽

T Cell Response ◽

Vitro Study

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IN VITRO STUDY ON THE IMMUNE RESPONSES OF PHAGOCYTIC CELLS INOCULATED WITH STAPHYLOCOCCUS AUREUS IN ASSOCIATION WITH ENROFLOXACIN TREATMENT

Assiut Veterinary Medical Journal ◽

10.21608/avmj.2013.171533 ◽

2013 ◽

Vol 59 (137) ◽

pp. 131-138

Keyword(s):

Staphylococcus Aureus ◽

Immune Responses ◽

In Vitro Study ◽

Vitro Study ◽

Phagocytic Cells

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Effect of electromagnetic field on acetylcholinesterase activity: In vitro study

African Journal of Biochemistry Research ◽

10.5897/ajbr11.088 ◽

2012 ◽

Vol 6 (1) ◽

Cited By ~ 1

Author(s):

Ezzatollah Fathi

Keyword(s):

Electromagnetic Field ◽

In Vitro Study ◽

Acetylcholinesterase Activity ◽

Vitro Study

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Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile

Stem Cells International ◽

10.1155/2017/4297639 ◽

2017 ◽

Vol 2017 ◽

pp. 1-16 ◽

Cited By ~ 9

Author(s):

Evelyn Lara ◽

Alejandra Velásquez ◽

Joel Cabezas ◽

Nathaly Rivera ◽

Paulina Pacha ◽

...

Keyword(s):

Stem Cells ◽

Immune Response ◽

Cell Proliferation ◽

Mesenchymal Stem Cells ◽

Inflammatory Mediator ◽

Adipogenic Differentiation ◽

Morphological Features ◽

Differentially Expressed ◽

Transcriptomic Profile

Mesenchymal stem cells (MSCs) were isolated and characterized from postpartum bovine endometrium of animals with subclinical (n=5) and clinical endometritis (n=3) and healthy puerperal females (n=5). Cells isolated displayed mean morphological features of MSCs and underwent osteogenic, chondrogenic, and adipogenic differentiation after induction (healthy and subclinical). Cells from cows with clinical endometritis did not undergo adipogenic differentiation. All cells expressed mRNAs for selected MSC markers. Endometrial MSCs were challenged in vitro with PGE2 at concentrations of 0, 1, 3, and 10 μM, and their global transcriptomic profile was studied. Overall, 1127 genes were differentially expressed between unchallenged cells and cells treated with PGE2 at all concentrations (763 up- and 364 downregulated, fold change > 2, and P<0.05). The pathways affected the most by the PGE2 challenge were immune response, angiogenesis, and cell proliferation. In conclusion, we demonstrated that healthy puerperal bovine endometrium contains MSCs and that endometritis modifies and limits some functional characteristics of these cells, such as their ability to proceed to adipogenic differentiation. Also, PGE2, an inflammatory mediator of endometritis, modifies the transcriptomic profile of endometrial MSCs. A similar situation may occur during inflammation associated with endometritis, therefore affecting the main properties of endometrial MSCs.

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