Ex Vivo and In Vivo Analysis of a Novel Porcine Aortic Patch for Vascular Reconstruction

(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus was to analyze the (ultra-) structure and the purification effort. (2) Methods: Established methodologies such as the histological material analysis and the conduct of the subcutaneous implantation model in Wistar rats were applied. Moreover, established methods combining histological, immunohistochemical, and histomorphometrical procedures were applied to analyze the tissue reactions to the vessel graft materials, including the induction of pro- and anti-inflammatory macrophages to test the immune response. (3) Results: The results showed that the PAP implants induced a special cellular infiltration and host tissue integration based on its three different parts based on the different layers of the donor tissue. Thereby, these material parts induced a vascularization pattern that branches to all parts of the graft and altogether a balanced immune tissue reaction in contrast to the control material. (4) Conclusions: PAP implants seemed to be advantageous in many aspects: (i) cellular infiltration and host tissue integration, (ii) vascularization pattern that branches to all parts of the graft, and (iii) balanced immune tissue reaction that can result in less scar tissue and enhanced integrative healing patterns. Moreover, the unique trans-implant vascularization can provide unprecedented anti-infection properties that can avoid material-related bacterial infections.

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Comparative In Vivo Analysis of the Integration Behavior and Immune Response of Collagen-Based Dental Barrier Membranes for Guided Bone Regeneration (GBR)

Membranes ◽

10.3390/membranes11090712 ◽

2021 ◽

Vol 11 (9) ◽

pp. 712

Author(s):

Milena Radenković ◽

Said Alkildani ◽

Ignacio Stoewe ◽

James Bielenstein ◽

Bernd Sundag ◽

...

Keyword(s):

Wound Healing ◽

Bone Regeneration ◽

Barrier Function ◽

Guided Bone Regeneration ◽

Collagen Membrane ◽

Cellular Infiltration ◽

Barrier Membranes ◽

Tissue Integration ◽

Tissue Reactions

Collagen-based resorbable barrier membranes have been increasingly utilized for Guided Bone Regeneration (GBR), as an alternative to non-resorbable synthetic membranes that require a second surgical intervention for removal. One of the most important characteristics of a resorbable barrier membrane is its mechanical integrity that is required for space maintenance and its tissue integration that plays a crucial role in wound healing and bone augmentation. This study compares a commercially available porcine-derived sugar-crosslinked collagen membrane with two non-crosslinked collagen barrier membranes. The material analysis provides an insight into the influence of manufacturing on the microstructure. In vivo subcutaneous implantation model provides further information on the host tissue reaction of the barrier membranes, as well as their tissue integration patterns that involve cellular infiltration, vascularization, and degradation. The obtained histochemical and immunohistochemical results over three time points (10, 30, and 60 days) showed that the tissue response to the sugar crosslinked collagen membrane involves inflammatory macrophages in a comparable manner to the macrophages observed in the surrounding tissue of the control collagen-based membranes, which were proven as biocompatible. The tissue reactions to the barrier membranes were additionally compared to wounds from a sham operation. Results suggest wound healing properties of all the investigated barrier membranes. However, the sugar-crosslinked membrane lacked in cellular infiltration and transmembraneous vascularization, providing an exclusive barrier function in GBR. Moreover, this membrane maintained a similar swelling ratio over examined timepoints, which suggests a very slow degradation pattern and supports its barrier function. Based on the study results, which showed biocompatibility of the sugar crosslinked membrane and its stability up to 60 days post-implantation, it can be concluded that this membrane may be suitable for application in GBR as a biomaterial with exclusive barrier functionality, similar to non-resorbable options.

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Evaluation of Host Tissue Integration, Revascularization, and Cellular Infiltration Within Various Dermal Substrates

Annals of Plastic Surgery ◽

10.1097/sap.0b013e31823b6b01 ◽

2012 ◽

Vol 68 (5) ◽

pp. 495-500 ◽

Cited By ~ 39

Author(s):

Anthony E. Capito ◽

Sunil S. Tholpady ◽

Hitesh Agrawal ◽

David B. Drake ◽

Adam J. Katz

Keyword(s):

Host Tissue ◽

Cellular Infiltration ◽

Tissue Integration

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Morphological Evaluation of the Tissue Reaction to Subcutaneous Implantation of Decellularized Matrices

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-018-4334-0 ◽

2018 ◽

Vol 166 (2) ◽

pp. 287-292

Author(s):

A. S. Sotnichenko ◽

R. Z. Nakokhov ◽

E. A. Gubareva ◽

E. V. Kuevda ◽

I. S. Gumenyuk

Keyword(s):

Tissue Reaction ◽

Subcutaneous Implantation ◽

Morphological Evaluation

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Local Immune Responses to Chlamydia pneumoniae in the Lungs of BALB/c Mice during Primary Infection and Reinfection

Infection and Immunity ◽

10.1128/iai.66.11.5113-5118.1998 ◽

1998 ◽

Vol 66 (11) ◽

pp. 5113-5118 ◽

Cited By ~ 46

Author(s):

Jenni M. Penttilä ◽

Marjukka Anttila ◽

Mirja Puolakkainen ◽

Aino Laurila ◽

Kari Varkila ◽

...

Keyword(s):

Primary Infection ◽

Bacterial Infections ◽

Chlamydia Pneumoniae ◽

Mononuclear Cells ◽

Relative Increase ◽

Culturable Bacteria ◽

Ifn Γ ◽

Tissue Reactions

ABSTRACT Cell-mediated immune (CMI) responses play a major role in protection as well as pathogenesis of many intracellular bacterial infections. In this study, we evaluated the infection kinetics and assessed histologically the lymphoid reactions and local, in vitro-restimulated CMI responses in lungs of BALB/c mice, during both primary infection and reinfection with Chlamydia pneumoniae. The primary challenge resulted in a self-restricted infection with elimination of culturable bacteria by day 27 after challenge. A mild lymphoid reaction characterized the pathology in the lungs. In vitro CMI responses consisted of a weak proliferative response and no secretion of gamma interferon (IFN-γ). The number of lung-derived mononuclear cells increased substantially during the primary infection; the largest relative increase was observed in B cells (B220+). After reinfection, the number of lung-derived mononuclear cells increased further, and the response consisted mainly of T cells. The reinfection was characterized in vivo by significant protection from infection (fewer cultivable bacteria in the lungs for a shorter period of time) but increased local lymphoid reaction at the infection site. In vitro, as opposed to the response in naive mice, acquired immunity was characterized by a strongly Th1-biased (IFN-γ) CMI response. These results suggest that repeated infections with C. pneumoniae may induce Th1-type responses with similar associated tissue reactions, as shown in C. trachomatis infection models.

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Optimizing the neuron-electrode interface for chronic bioelectronic interfacing

Neurosurgical FOCUS ◽

10.3171/2020.4.focus20178 ◽

2020 ◽

Vol 49 (1) ◽

pp. E7

Author(s):

Conor Keogh

Keyword(s):

Electrode Materials ◽

Neurological Diseases ◽

Tissue Reaction ◽

Biological Interactions ◽

Implanted Electrodes ◽

Tissue Integration ◽

Closed Loop Systems ◽

Electrode Interface ◽

Neural Interfacing

Engineering approaches have vast potential to improve the treatment of disease. Brain-machine interfaces have become a well-established means of treating some otherwise medically refractory neurological diseases, and they have shown promise in many more areas. More widespread use of implanted stimulating and recording electrodes for long-term intervention is, however, limited by the difficulty in maintaining a stable interface between implanted electrodes and the local tissue for reliable recording and stimulation.This loss of performance at the neuron-electrode interface is due to a combination of inflammation and glial scar formation in response to the implanted material, as well as electrical factors contributing to a reduction in function over time. An increasing understanding of the factors at play at the neural interface has led to greater focus on the optimization of this neuron-electrode interface in order to maintain long-term implant viability.A wide variety of approaches to improving device interfacing have emerged, targeting the mechanical, electrical, and biological interactions between implanted electrodes and the neural tissue. These approaches are aimed at reducing the initial trauma and long-term tissue reaction through device coatings, optimization of mechanical characteristics for maximal biocompatibility, and implantation techniques. Improved electrode features, optimized stimulation parameters, and novel electrode materials further aim to stabilize the electrical interface, while the integration of biological interventions to reduce inflammation and improve tissue integration has also shown promise.Optimization of the neuron-electrode interface allows the use of long-term, high-resolution stimulation and recording, opening the door to responsive closed-loop systems with highly selective modulation. These new approaches and technologies offer a broad range of options for neural interfacing, representing the possibility of developing specific implant technologies tailor-made to a given task, allowing truly personalized, optimized implant technology for chronic neural interfacing.

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In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

Materials ◽

10.3390/ma12071186 ◽

2019 ◽

Vol 12 (7) ◽

pp. 1186

Author(s):

Lívia da Costa Pereira ◽

Carlos Fernando de Almeida Barros Mourão ◽

Adriana Terezinha Neves Novellino Alves ◽

Rodrigo Figueiredo de Brito Resende ◽

Marcelo José Pinheiro Guedes de Uzeda ◽

...

Keyword(s):

Bone Regeneration ◽

Chemical Properties ◽

Chemical Characterization ◽

Guided Bone Regeneration ◽

Tissue Reaction ◽

In Vivo Evaluation ◽

Physico Chemical ◽

Tissue Reactions

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

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Midterm outcomes of total hip arthroplasty with a modular Birmingham head

Hip International ◽

10.1177/1120700019877389 ◽

2019 ◽

pp. 112070001987738 ◽

Cited By ~ 1

Author(s):

Duncan W Cushnie ◽

Brent A Lanting ◽

Richard McCalden ◽

Douglas DR Naudie ◽

James L Howard

Keyword(s):

Soft Tissue ◽

Total Hip Arthroplasty ◽

Hip Arthroplasty ◽

Femoral Stem ◽

Tissue Reaction ◽

Harris Hip Score ◽

Total Hip ◽

Tissue Reactions ◽

Clinical Records

Introduction: Birmingham Hip Resurfacing (BHR) implants may be combined with a conventional femoral stem to create a modular metal-on-metal total hip arthroplasty (BHR MoM THA). There is little outcome data regarding this construct. This study examines midterm outcomes of BHR MoM THA compared to oxidised zirconium total hip arthroplasty (THA). Methods: A retrospective institutional review identified all patients receiving BHR MoM THA between April 2005 and February 2011 and a matched control cohort of zirconium THA patients. Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Harris Hip Score (HHS), and SF-12 Health status scores were obtained. Revisions and complications were collected from clinical records. Radiographs were assessed for evidence of component malposition, loosening, osteolysis, or heterotopic ossification. Results: 63 modular BHR MoM THA were identified in 61 patients (36 with BHR cups, 27 with R3 cups) and 63 zirconium THA in 58 matched controls. Mean follow-up was 58 months. 14 BHR MoM THA hips (22.2%) were revised (4 infections, 1 dislocation, 9 soft tissue reactions) compared to 3 (4.8%) zirconium THA (all infections). At latest follow-up, 18.4% of surviving BHR MoM THA hips were painful compared to 0.5% of zirconium THA controls ( p < 0.001). WOMAC, HHS, and SF-12 did not differ significantly between surviving members of the 2 groups. Discussion: BHR MoM THA demonstrated a high revision rate, largely for adverse local soft tissue reaction and pain. Among those not revised, many reported some residual pain despite similar quality of life measures to those who received zirconium THA.

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Tissue Reaction of the Rat Urinary Bladder to Synthetic Mesh Materials

The Scientific World JOURNAL ◽

10.1100/tsw.2009.120 ◽

2009 ◽

Vol 9 ◽

pp. 1046-1051 ◽

Cited By ~ 1

Author(s):

Gokhan Atis ◽

Serdar Arisan ◽

Aysim Ozagari ◽

Turhan Caskurlu ◽

Ayhan Dalkilinc ◽

...

Keyword(s):

Foreign Body ◽

Urinary Bladder ◽

Foreign Body Reaction ◽

Bladder Wall ◽

Synthetic Mesh ◽

Tissue Reaction ◽

Collagen Deposition ◽

Rat Urinary Bladder ◽

Intravaginal Slingplasty ◽

Tissue Reactions

The aim of this study was to assess urinary bladder histopathology induced by the sling materials tension-free vaginal tape (TVT), vypro mesh, and intravaginal slingplasty (IVS). Thirty rats were studied: sham-operated controls, TVT, vypro, and IVS groups. After laparotomy, a 0.5- x 1-cm piece of mesh was implanted on the anterior bladder wall. The bladder was examined histopathologically after 12 weeks. Inflammation, foreign-body reaction, subserosal fibrosis, necrosis, and collagen deposition were graded. The Kruskal-Wallis and posthoc Dunn tests were used. The sham-operated rats showed no tissue reactions. The TVT, vypro, and IVS groups showed increased inflammation (p= 0.006,p= 0.031,p= 0.001), subserosal fibrosis (p= 0.0001), foreign-body reaction (p = 0.0001), and collagen deposition (p= 0.0001) as compared to sham. Inflammation was more intense in the IVS group as compared to the TVT and vypro groups (p= 0.041,p= 0.028). The bladder presented more increased inflammatory response to IVS than the other meshs. This may play a role in the ultimate outcomes or complications from slings.

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ERK1/2 Activation Induced by Inflammatory Cytokines Compromises Effective Host Tissue Integration of Engineered Cartilage

Tissue Engineering Part A ◽

10.1089/ten.tea.2008.0663 ◽

2009 ◽

Vol 15 (10) ◽

pp. 2825-2835 ◽

Cited By ~ 24

Author(s):

Farida Djouad ◽

Lars Rackwitz ◽

Yingjie Song ◽

Sasa Janjanin ◽

Rocky S. Tuan

Keyword(s):

Inflammatory Cytokines ◽

Host Tissue ◽

Tissue Integration ◽

Engineered Cartilage

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Immunohistochemical observation of local inflammatory cell infiltration in the host-tissue reaction site of human hydatid cysts

Journal of Helminthology ◽

10.1017/s0022149x1800024x ◽

2018 ◽

Vol 93 (3) ◽

pp. 277-285 ◽

Cited By ~ 1

Author(s):

R. Jafari ◽

B. Sanei ◽

A. Baradaran ◽

M. Kolahdouzan ◽

B. Bagherpour ◽

...

Keyword(s):

T Cells ◽

Immune Cells ◽

Immune Cell ◽

Tissue Reaction ◽

Distinct Pattern ◽

Host Tissue ◽

Cell Infiltration ◽

Reaction Site ◽

Hydatid Cysts ◽

Ki 67

AbstractThe aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis. In the host-tissue reaction site of liver hydatid cysts, a distinct pattern of local immune cell response, which outwardly consisted of a pack of the fibrous elements, a layer of palisading macrophages, an eosinophil-containing layer and a layer of accumulated lymphocytes, was observed. However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells. The CD3+ T cells were the predominant inflammatory cells in all groups, followed by CD19+ B cells. It can be concluded that different immune cells are involved in the local response to human hydatid cysts.

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