scholarly journals ERK1/2 Activation Induced by Inflammatory Cytokines Compromises Effective Host Tissue Integration of Engineered Cartilage

2009 ◽  
Vol 15 (10) ◽  
pp. 2825-2835 ◽  
Author(s):  
Farida Djouad ◽  
Lars Rackwitz ◽  
Yingjie Song ◽  
Sasa Janjanin ◽  
Rocky S. Tuan
Keyword(s):  
Inflammatory Cytokines ◽  
Host Tissue ◽  
Tissue Integration ◽  
Engineered Cartilage

scholarly journals Development of a composite acellular tissue graft for musculoskeletal tissue engineering

2015 ◽  
Author(s):  
◽  
Sarah Elizabeth Smith
Keyword(s):  
Tissue Engineering ◽  
Gold Nanoparticles ◽  
Acl Reconstruction ◽  
Host Tissue ◽  
Hydroxyapatite Nanoparticles ◽  
Musculoskeletal Tissue ◽  
Tissue Integration ◽  
Composite Grafts ◽  
Acellular Tissue

A composite acellular tissue graft comprised of decellularized tendon conjugated with nanomaterials has been developed for musculoskeletal tissue engineering applications. The focus of this dissertation is on the development of composite grafts derived from decellularized human tendon conjugated with gold nanoparticles and hydroxyapatite nanoparticles for use in anterior cruciate ligament (ACL) reconstruction. Gold nanoparticles are used to promote remodeling, cellularity, and biological incorporation of grafts. Hydroxyapatite nanoparticles are used to promote osseointegration, cellularity, and to enhance the graft/bone interface. These composite grafts along with several other variations, were characterized in vitro using a variety of cell-based assays including cell viability, cell proliferation, and cell migration assays. Two in vivo studies were conducted. A green fluorescent protein (GFP) porcine model was investigated as a new method to evaluate host tissue integration into soft tissue grafts as well as the in vivo biocompatibility of subcutaneously implanted composite grafts. Results demonstrate biocompatibility and remodeling of composite grafts and the value of using the GFP model as a qualitative method for assessing host tissue integration. A rabbit ACL reconstruction model was used to investigate graft remodeling in addition to the overall viability of using composite grafts to serve as a functional ACL replacement. Results demonstrate successful replacement of ACLs using composite grafts with enhanced remodeling from the addition of nanoparticles. Overall, studies demonstrate the success and potential further application of using composite grafts for musculoskeletal tissue engineering applications. Future studies will include expanding development of variations of these composite materials to address additional clinical needs.

scholarly journals Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines

2018 ◽  
Vol 36 (11) ◽  
pp. 2901-2910 ◽  
Author(s):  
Bhavana Mohanraj ◽  
Alice H. Huang ◽  
Meira J. Yeger-McKeever ◽  
Megan J. Schmidt ◽  
George R. Dodge ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Inflammatory Cytokines ◽  
Differential Sensitivity ◽  
Engineered Cartilage ◽  
Pro Inflammatory Cytokines

scholarly journals The Use of a Green Fluorescent Protein Porcine Model to Evaluate Host Tissue Integration into Extracellular Matrix Derived Bionanocomposite Scaffolds

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
S. E. Smith ◽  
R. A. White ◽  
D. A. Grant ◽  
S. A. Grant
Keyword(s):  
Extracellular Matrix ◽  
Green Fluorescent Protein ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Fluorescent Protein ◽  
Host Tissue ◽  
Soft Tissue Repair ◽  
Tissue Integration ◽  
Tissue Migration ◽  
Green Fluorescent

When using heterogeneous extracellular matrix (ECM) derived scaffolds for soft tissue repair, current methods of in vivo evaluation can fail to provide a clear distinction between host collagen and implanted scaffolds making it difficult to assess host tissue integration and remodeling. The purpose of this study is both to evaluate novel scaffolds conjugated with nanoparticles for host tissue integration and biocompatibility and to assess green fluorescent protein (GFP) expressing swine as a new animal model to evaluate soft tissue repair materials. Human-derived graft materials conjugated with nanoparticles were subcutaneously implanted into GFP expressing swine to be evaluated for biocompatibility and tissue integration through histological scoring and confocal imaging. Histological scoring indicates biocompatibility and remodeling of the scaffolds with and without nanoparticles at 1, 3, and 6 months. Confocal microscope images display host tissue integration into scaffolds although nonspecificity of GFP does not allow for quantification of integration. However, the confocal images do allow for spatial observation of host tissue migration into the scaffolds at different depths of penetration. The study concludes that the nanoparticle scaffolds are biocompatible and promote integration and that the use of GFP expressing swine can aid in visualizing the scaffold/host interface and host cell/tissue migration.

scholarly journals Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Jiaxi Chen ◽  
Huiqun Zhou ◽  
Daping Xie ◽  
Yiming Niu
Keyword(s):  
Foreign Body ◽  
Pore Size ◽  
Foreign Body Reaction ◽  
Inflammatory Cells ◽  
Host Tissue ◽  
Bletilla Striata ◽  
Pore Sizes ◽  
Tissue Integration

Abstract Background Implantation of a biomaterial may induce the foreign-body reaction to the host tissue that determines the outcome of the integration and the biological performance of the implants. The foreign-body reaction can be modulated by control of the material properties of the implants. Methods First, we synthesized methacrylated Bletilla striata Polysaccharide (BSP-MA) and constructed a series of open porous cryogels utilizing this material via the freezing-thawing treatment of solvent-precursors systems. Second, Pore size and modulus were measured to characterize the properties of BSP cryogels. Live/dead staining of cells and CCK-8 were performed to test the cytocompatibility of the scaffolds. In addition, the Real-Time qPCR experiments were carried for the tests. Finally, the BSP scaffolds were implanted subcutaneously to verify the foreign-body reaction between host tissue and materials. Results Our data demonstrated that cryogels with different pore sizes and modulus can be fabricated by just adjusting the concentration. Besides, the cryogels showed well cytocompatibility in the in vitro experiments and exhibited upregulated expression levels of pro-inflammation-related genes (Tnfa and Il1b) with the increase of pore size. In vivo experiments further proved that with the increase of pore size, more immune cells infiltrated into the inner zone of materials. The foreign-body reaction and the distribution of immune-regulatory cells could be modulated by tuning the material microstructure. Conclusions Collectively, our findings revealed Bletilla striata polysaccharide cryogel scaffold with different pore sizes can spatially control foreign-body reaction. The microstructure of cryogels could differentially guide the distribution of inflammatory cells, affect the formation of blood vessels and fibrous capsules, which eventually influence the material-tissue integration. This work demonstrates a practical strategy to regulate foreign body reaction and promote the performance of medical devices.

scholarly journals Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

RSC Advances ◽  
2016 ◽  
Vol 6 (92) ◽  
pp. 89347-89355 ◽  
Author(s):  
S. Aguayo ◽  
A. Strange ◽  
N. Gadegaard ◽  
M. J. Dalby ◽  
L. Bozec
Keyword(s):  
Staphylococcus Aureus ◽  
Elastic Properties ◽  
Host Tissue ◽  
Bacterial Colonisation ◽  
Beneficial Effects ◽  
Tissue Integration ◽  
Nanoscale Topography

Despite the well-known beneficial effects of biomaterial nanopatterning on host tissue integration, the influence of controlled nanoscale topography on bacterial colonisation and infection remains unknown.

Evaluation of Host Tissue Integration, Revascularization, and Cellular Infiltration Within Various Dermal Substrates

2012 ◽  
Vol 68 (5) ◽  
pp. 495-500 ◽  
Author(s):  
Anthony E. Capito ◽  
Sunil S. Tholpady ◽  
Hitesh Agrawal ◽  
David B. Drake ◽  
Adam J. Katz
Keyword(s):  
Host Tissue ◽  
Cellular Infiltration ◽  
Tissue Integration

scholarly journals DNA Polyelectrolyte Multilayer Coatings Are Antifouling and Promote Mammalian Cell Adhesion

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4596
Author(s):  
Omar Abdelaziz Ouni ◽  
Guruprakash Subbiahdoss ◽  
Andrea Scheberl ◽  
Erik Reimhult
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Bone Cells ◽  
Cell Attachment ◽  
Host Tissue ◽  
Tissue Cell ◽  
Dual Function ◽  
Multilayer Coatings ◽  
Polyelectrolyte Multilayer ◽  
Tissue Integration

The ability of bacteria to adhere to and form biofilms on implant surfaces is the primary cause of implant failure. Implant-associated infections are difficult to treat, as the biofilm mode of growth protects microorganisms from the host’s immune response and antibiotics. Therefore, modifications of implant surfaces that can prevent or delay bacterial adhesion and biofilm formation are highly desired. In addition, the attachment and spreading of bone cells are required for successful tissue integration in orthopedic and dental applications. We propose that polyanionic DNA with a negatively charged phosphate backbone could provide a dual function to repel bacterial adhesion and support host tissue cell attachment. To this end, we developed polyelectrolyte multilayer coatings using chitosan (CS) and DNA on biomaterial surfaces via a layer-by-layer technique. The assembly of these coatings was characterized. Further, we evaluated staphylococcal adhesion and biofilm growth on the coatings as well as cytotoxicity for osteoblast-like cells (SaOS-2 cells), and we correlated these to the layer structure. The CS-DNA multilayer coatings impaired the biofilm formation of Staphylococcus by ~90% on both PMMA and titanium surfaces. The presence of cationic CS as the top layer did not hinder the bacteria-repelling property of the DNA in the coating. The CS-DNA multilayer coatings demonstrated no cytotoxic effect on SaOS-2 cells. Thus, DNA polyelectrolyte multilayer coatings could reduce infection risk while promoting host tissue cell attachment on medical implants.

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