Morphological Evaluation of the Tissue Reaction to Subcutaneous Implantation of Decellularized Matrices

2018 ◽  
Vol 166 (2) ◽  
pp. 287-292
Author(s):  
A. S. Sotnichenko ◽  
R. Z. Nakokhov ◽  
E. A. Gubareva ◽  
E. V. Kuevda ◽  
I. S. Gumenyuk
Keyword(s):  
Tissue Reaction ◽  
Subcutaneous Implantation ◽  
Morphological Evaluation

Soft tissue reaction to collagen-immobilized porous polyethylene: subcutaneous implantation in rats for 20 wk

Biomaterials ◽  
1993 ◽  
Vol 14 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Yukihiko Kinoshita ◽  
Takeshi Kuzuhara ◽  
Mitsuhiro Kirigakubo ◽  
Masaru Kobayashi ◽  
Kaizou Shimura ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Tissue Reaction ◽  
Subcutaneous Implantation ◽  
Porous Polyethylene ◽  
Soft Tissue Reaction

Tissue reaction to subcutaneous implantation of a collagen sponge. A histological, ultrastructural, and immunological study

1990 ◽  
Vol 24 (6) ◽  
pp. 689-703 ◽  
Author(s):  
K. Anselme ◽  
C. Bacques ◽  
G. Charriere ◽  
D. J. Hartmann ◽  
D. Herbage ◽  
...  
Keyword(s):  
Tissue Reaction ◽  
Collagen Sponge ◽  
Immunological Study ◽  
Subcutaneous Implantation

scholarly journals Assessment of carvacrol paste as endodontic medication associated with laser photobiomodulation in tissue repair: an experimental study in rats

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Ismário Silva Meneses ◽  
Ricardo Luiz Cavalcanti de Albuquerque Júnior ◽  
Felipe de Souza Matos ◽  
Aline Aragão Pereira Macedo ◽  
Adriano Antunes de Souza Araújo ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Wistar Rats ◽  
Type I Collagen ◽  
Subcutaneous Tissue ◽  
Fibrous Tissue ◽  
Tissue Reaction ◽  
Type Iii Collagen ◽  
Type I ◽  
Subcutaneous Implantation ◽  
Inflammatory Infiltration

This study aimed to analyze the tissue reaction caused by carvacrol paste associated or not with laser photobiomodulation (LPBM) at λ660 nm in the subcutaneous tissue of rats. Sixty Wistar rats were divided into four groups and they received the following interventions: subcutaneous implantation of empty polyethylene tubes (CTR), implantation of tubes containing carvacrol paste (CVC), implantation of empty tubes and LPBM (LLLT), and implantation of tubes containing carvacrol paste and LPBM (CVCLT). The animals were euthanized at three, eight, and 15 days after surgery. The inflammatory reaction and fibroplasia were analyzed histomorphometrically. Significant differences among the groups were determined by ANOVA and Tukey's test (p<0.05). In the 3-day period, the CVCLT group had low inflammatory infiltration (p<0.01). In the 8- and 15-day periods, the LLLT and CVCLT groups presented a low amount of lymphocytic inflammatory infiltrate (p<0.01 and p<0.05). Regarding the formation of fibrous tissue, the CVC group had the highest formation of type III collagen in the 8-day period (p<0.001). In the 15-day period, the CVCLT group had a lower formation of type I collagen than the CTR and LLLT groups (p<0.05). The use of the carvacrol paste associated with photobiomodulation optimizes the inflammatory period and tissue repair.

scholarly journals FiltekTM Silorane and FiltekTM Supreme XT resins: tissue reaction after subcutaneous implantation in isogenic mice

2011 ◽  
Vol 22 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Esther Ruiz De Castañeda ◽  
Léa A. B. Silva ◽  
Patrícia Gaton-Hernández ◽  
Alberto Consolaro ◽  
Encarnación Gonzalez Rodriguez ◽  
...  
Keyword(s):  
Microscopic Analysis ◽  
Experimental Period ◽  
Tissue Reaction ◽  
Fiber Formation ◽  
Tissue Response ◽  
Control Group ◽  
Subcutaneous Implantation ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

The aim of this study was to evaluate the tissue compatibility of a silorane-based resin system (FiltekTM Silorane) and a methacrylate-based nanoparticle resin (FiltekTM Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice. One hundred and thirty five male isogenic BALB/c mice were randomly assigned to 12 experimental and 3 control groups, according to the implanted material and the experimental period of 7, 21 and 63 days. At the end of each period, the animals were killed and the tubes with the surrounding tissues were removed and processed for microscopic analysis. Samples were subjected to a descriptive and a semi-quantitative analyses using a 4-point scoring system (0-3) to evaluate the collagen fiber formation and inflammatory infiltrate. Data were statistically analyzed using the Kruskal Wallis test (?=0.05). The results showed that there was no significant difference between the experimental and control groups considering the three evaluation periods (p>0.05). The silorane-based and the methacrylate-based nanoparticle resins presented similar tissue response to that of the empty tube (control group) after subcutaneous implantation in isogenic mice.

scholarly journals Ex Vivo and In Vivo Analysis of a Novel Porcine Aortic Patch for Vascular Reconstruction

2021 ◽  
Vol 22 (14) ◽  
pp. 7623
Author(s):  
Ignacio Stöwe ◽  
Jens Pissarek ◽  
Pia Moosmann ◽  
Annica Pröhl ◽  
Sven Pantermehl ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Tissue Reaction ◽  
Host Tissue ◽  
Graft Material ◽  
Cellular Infiltration ◽  
Subcutaneous Implantation ◽  
Tissue Integration ◽  
Immune Tissue ◽  
Tissue Reactions ◽  
Implantation Model

(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus was to analyze the (ultra-) structure and the purification effort. (2) Methods: Established methodologies such as the histological material analysis and the conduct of the subcutaneous implantation model in Wistar rats were applied. Moreover, established methods combining histological, immunohistochemical, and histomorphometrical procedures were applied to analyze the tissue reactions to the vessel graft materials, including the induction of pro- and anti-inflammatory macrophages to test the immune response. (3) Results: The results showed that the PAP implants induced a special cellular infiltration and host tissue integration based on its three different parts based on the different layers of the donor tissue. Thereby, these material parts induced a vascularization pattern that branches to all parts of the graft and altogether a balanced immune tissue reaction in contrast to the control material. (4) Conclusions: PAP implants seemed to be advantageous in many aspects: (i) cellular infiltration and host tissue integration, (ii) vascularization pattern that branches to all parts of the graft, and (iii) balanced immune tissue reaction that can result in less scar tissue and enhanced integrative healing patterns. Moreover, the unique trans-implant vascularization can provide unprecedented anti-infection properties that can avoid material-related bacterial infections.

Techniques For The Preparation of Tissue Samples Containing Injectable Polymer Microcapsules

1985 ◽  
Vol 43 ◽  
pp. 710-711
Author(s):  
G.E. Visscher ◽  
R. L. Robison ◽  
G. J. Argentieri
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Gastrocnemius Muscle ◽  
Degradation Process ◽  
Delivery Systems ◽  
Tissue Reaction ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Injectable Polymer ◽  
Microscopic Techniques

The use of various bioerodable polymers as drug delivery systems has gained considerable interest in recent years. Among some of the shapes used as delivery systems are films, rods and microcapsules. The work presented here will deal with the techniques we have utilized for the analysis of the tissue reaction to and actual biodegradation of injectable microcapsules. This work has utilized light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques. The design of our studies has utilized methodology that would; 1. best characterize the actual degradation process without artifacts introduced by fixation procedures and 2. allow for reproducible results.In our studies, the gastrocnemius muscle of the rat was chosen as the injection site. Prior to the injection of microcapsules the skin above the sites was shaved and tattooed for later recognition and recovery. 1.0 cc syringes were loaded with the desired quantity of microcapsules and the vehicle (0.5% hydroxypropylmethycellulose) drawn up. The syringes were agitated to suspend the microcapsules in the injection vehicle.

Distribution of VLA-5 and VLA-4 fibronectin receptors in human monocytes demonstrated by immunofluorescence, immunocytochemistry, and autoradiography

1993 ◽  
Vol 51 ◽  
pp. 306-307
Author(s):  
Robert Williams ◽  
Che-Hung Lee ◽  
Sara E. Quella ◽  
David M. Harlan ◽  
Yuan-Hsu Kang
Keyword(s):  
Present Report ◽  
Matrix Proteins ◽  
Extracellular Matrices ◽  
Human Monocytes ◽  
Integrin Receptors ◽  
Morphological Evaluation ◽  
The Matrix ◽  
Specific Receptors ◽  
Cytokine Stimulation ◽  
Monocyte Adherence

Monocyte adherence to endothelial or extracellular matrices plays an important role in triggering monocyte activation in extravascular sites of infection, chronic inflammatory disorders, and tissue damage. Migration of monocytes in the tissues involves the response to a chemoattractant and movement by a series of attachments and detachments to the extracellular matrices which are regulated by expression and distribution of specific receptors for the matrix proteins such as fibronectin (FN). The VSAs (very late antigens or beta integrins), a subfamily of the transmembrane heterodimeric integrin receptors, have been thought to play a major role in monocyte adherence to the extracellular matrices and cells. In this subfamily, VLA-5 and VLA-4 are believed to be the most essential integrins mediating monocyte adherence to FN. In the present report, we have established and compared different procedures for morphological evaluation of the expression and distribution of the FN receptors on human monocytes in order to investigate their response to endotoxin or cytokine stimulation.

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