scholarly journals Anti-Melanoma Capability of Contactless Cold Atmospheric Plasma Treatment

2021 ◽  
Vol 22 (21) ◽  
pp. 11728
Author(s):  
Dayun Yan ◽  
Qihui Wang ◽  
Xiaoliang Yao ◽  
Alisa Malyavko ◽  
Michael Keidar
Keyword(s):  
Tumor Therapy ◽  
Treatment Method ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Operational Parameter ◽  
Cold Atmospheric Plasma ◽  
Non Invasive ◽  
Metal Sheets ◽  
Physically Based ◽  
Solid Foundation

In this study, we demonstrated that the widely used cold atmospheric plasma (CAP) jet could significantly inhibit the growth of melanoma cells using a contactless treatment method, The flow rate of helium gas was a key operational parameter to modulate electromagnetic (EM) effect on melanoma cells. Metal sheets with different sizes could be used as a strategy to control the strength of EM effect. More attractive, the EM effect from CAP could penetrate glass/polystyrene barriers as thick as 7 mm. All these discoveries presented the profound non-invasive nature of a physically based CAP treatment, which provided a solid foundation for CAP-based cutaneous/subcutaneous tumor therapy.

Cold atmospheric plasma, a new strategy to induce senescence in melanoma cells

2013 ◽  
Vol 22 (4) ◽  
pp. 284-289 ◽  
Author(s):  
Stephanie Arndt ◽  
Eva Wacker ◽  
Yang-Fang Li ◽  
Tetsuji Shimizu ◽  
Hubertus M. Thomas ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
New Strategy

scholarly journals Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Christin Schneider ◽  
Lisa Gebhardt ◽  
Stephanie Arndt ◽  
Sigrid Karrer ◽  
Julia L. Zimmermann ◽  
...  
Keyword(s):  
Calcium Influx ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals The Hyaluronan Pericellular Coat and Cold Atmospheric Plasma Treatment of Cells

2020 ◽  
Vol 10 (15) ◽  
pp. 5024
Author(s):  
Claudia Bergemann ◽  
Anna-Christin Waldner ◽  
Steffen Emmert ◽  
J. Barbara Nebe
Keyword(s):  
Plasma Treatment ◽  
Laser Scanning ◽  
Tumor Therapy ◽  
Impedance Measurement ◽  
Laser Scanning Microscopy ◽  
Atmospheric Plasma ◽  
Confocal Laser ◽  
Hacat Cells ◽  
Cold Atmospheric Plasma ◽  
Medicine Research

In different tumors, high amounts of hyaluronan (HA) are correlated with tumor progression. Therefore, new tumor therapy strategies are targeting HA production and degradation. In plasma medicine research, antiproliferative and apoptosis-inducing effects on tumor cells were observed using cold atmospheric plasma (CAP) or plasma-activated media (PAM). Until now, the influence of PAM on the HA pericellular coat has not been the focus of research. PAM was generated by argon-plasma treatment of Dulbecco’s modified Eagle’s Medium via the kINPen®09 plasma jet. The HA expression on PAM-treated HaCaT cells was determined by flow cytometry and confocal laser scanning microscopy. Changes in the adhesion behavior of vital cells in PAM were observed by impedance measurement using the xCELLigence system. We found that PAM treatment impaired the HA pericellular coat of HaCaT cells. The time-dependent adhesion was impressively diminished. However, a disturbed HA coat alone was not the reason for the inhibition of cell adhesion because cells enzymatically treated with HAdase did not lose their adhesion capacity completely. Here, we showed for the first time that the plasma-activated medium (PAM) was able to influence the HA pericellular coat.

scholarly journals Cold Atmospheric Plasma Is a Potent Tool to Improve Chemotherapy in Melanoma In Vitro and In Vivo

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1011 ◽  
Author(s):  
Mina Alimohammadi ◽  
Monireh Golpour ◽  
Farshad Sohbatzadeh ◽  
Seyedehniaz Hadavi ◽  
Sander Bekeschus ◽  
...  
Keyword(s):  
Scientific Evidence ◽  
Argon Plasma ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Tumor Control ◽  
Substantial Impact ◽  
L929 Cells ◽  
Cold Atmospheric Plasma

Malignant melanoma is a devastating disease. Because of its aggressiveness, it also serves as a model tumor for investigating novel therapeutic avenues. In recent years, scientific evidence has shown that cold atmospheric plasma (CAP) might be a promising modality in cancer therapy. In this study, we aimed to evaluate the effect of CAP generated by an argon plasma jet alone or in combination with dacarbazine (DAC) on melanoma cells in vitro and in vivo. The effects of the CAP on inducing lipid peroxidation and nitric oxide production were higher in B16 melanoma cells in comparison to non-malignant L929 cells. Assays on cell growth, apoptosis, and expression of genes related to, e.g., autophagic processes, showed CAP to have a substantial impact in melanoma cells while there were only minoreffects in L929 cells. In vivo, both CAP monotherapy and combination with DAC significantly decreased tumor growth. These results suggest that CAP not only selectively induces cell death in melanoma but also holds promises in combination with chemotherapy that might lead to improved tumor control.

Cold atmospheric plasma (CAP) as a promising therapeutic option for mild to moderate acne vulgaris: Clinical and non-invasive evaluation of two cases

2020 ◽  
Vol 19-20 ◽  
pp. 100110
Author(s):  
Dr. Arisi Mariachiara ◽  
Venturuzzo Anna ◽  
Gelmetti Alessandra ◽  
Guasco Pisani Edoardo ◽  
Bassissi Stefania ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Acne Vulgaris ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Non Invasive ◽  
Invasive Evaluation

scholarly journals Acidified Nitrite Contributes to the Antitumor Effect of Cold Atmospheric Plasma on Melanoma Cells

2021 ◽  
Vol 22 (7) ◽  
pp. 3757
Author(s):  
Tom Zimmermann ◽  
Lisa A Gebhardt ◽  
Lucas Kreiss ◽  
Christin Schneider ◽  
Stephanie Arndt ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Membrane Damage ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Active Components ◽  
Antitumor Effects ◽  
Cold Atmospheric Plasma ◽  
Viability Analysis ◽  
Cellular Effects ◽  
The Impact

Cold atmospheric plasma (CAP) is partially ionized gas near room temperature with previously reported antitumor effects. Despite extensive research and growing interest in this technology, active components and molecular mechanisms of CAP are not fully understood to date. We used Raman spectroscopy and colorimetric assays to determine elevated nitrite and nitrate levels after treatment with a MiniFlatPlaster CAP device. Previously, we demonstrated CAP-induced acidification. Cellular effects of nitrite and strong extracellular acidification were assessed using live-cell imaging of intracellular Ca2+ levels, cell viability analysis as well as quantification of p21 and DNA damage. We further characterized these observations by analyzing established molecular effects of CAP treatment. A synergistic effect of nitrite and acidification was found, leading to strong cytotoxicity in melanoma cells. Interestingly, protein nitration and membrane damage were absent after treatment with acidified nitrite, thereby challenging their contribution to CAP-induced cytotoxicity. Further, phosphorylation of ERK1/2 was increased after treatment with both acidified nitrite and indirect CAP. This study characterizes the impact of acidified nitrite on melanoma cells and supports the importance of RNS during CAP treatment. Further, it defines and evaluates important molecular mechanisms that are involved in the cancer cell response to CAP.

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