scholarly journals An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

2021 ◽  
Vol 22 (21) ◽  
pp. 11755
Author(s):  
Guotao Mao ◽  
Kai Wang ◽  
Fangyuan Wang ◽  
Hao Li ◽  
Hongsen Zhang ◽  
...  
Keyword(s):  
Malachite Green ◽  
Laccase Activity ◽  
Synthetic Dyes ◽  
Dyeing Wastewater ◽  
Great Ability ◽  
Conserved Residues ◽  
Ph Values ◽  
Malachite Green Dye ◽  
Industrial Use ◽  
Biochemical Analyses

Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from Geothermobacter hydrogeniphilus (Ghlac)was cloned and expressed in Escherichia coli. Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and Zea mays. In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater.

scholarly journals Optimizing Laccase Production in Auricularia Cornea by Submerged Fermentation With Wheat Bran Extract: Applications in Decolorization of Malachite Green Dye

2021 ◽  
Author(s):  
Li Meng ◽  
Xiaoran Bai ◽  
Qingji Wang ◽  
Xiaobo Li ◽  
Shaoyan Zhang ◽  
...  
Keyword(s):  
Malachite Green ◽  
Wheat Bran ◽  
Submerged Fermentation ◽  
High Performance ◽  
Laccase Activity ◽  
Degradation Products ◽  
Laccase Production ◽  
Malachite Green Dye ◽  
Chromosome Bridge ◽  
Wheat Bran Extract

Abstract Wheat bran extract may enhance laccase activity of Auricularia cornea in submerged fermentation. The laccase activity of A. cornea was maximal (768.78 U/mL) at 26.9 ℃, pH 5, a time point of 6 d, 22 h, and an inducer concentration of 10%. Laccase from A. cornea AC5 decolorized 83.27% of 75 mg/L malachite green within 12 h. High performance liquid chromatography (HPLC) analysis of the extracted metabolites suggested that the decolorization occurred through biodegradation. Malachite green induced cytotoxic damage including formation of a micronucleus and chromosome bridge at anaphase. Degradation products of malachite green proved to be less toxic and had negligible effects on chromosomal aberrations.

The Malachite Green Biodegradation in Bioreactors on Various pH Domains

2019 ◽  
Vol 70 (8) ◽  
pp. 2996-2999
Author(s):  
Viorel Gheorghe ◽  
Catalina Gabriela Gheorghe ◽  
Andreea Bondarev ◽  
Vasile Matei ◽  
Mihaela Bombos
Keyword(s):  
Malachite Green ◽  
Contact Time ◽  
Dominant Species ◽  
Wastewater Treatment Plants ◽  
Organic Substances ◽  
Biological Degradation ◽  
Growth Promoters ◽  
Ph Values ◽  
Biological Sludge ◽  
Ph Range

In the experimental study was studied the malachite green colorant biodegradation in biological sludge with biological activity. The biodegradability tests were carried out in laboratory bioreactors, on aqueous solutions of green malachite contacted with microorganisms in which the dominant species is Paramecium caudatum, in a pH range between 8 and 12, temperatures in the ranges 25-350C, using pH neutralizing substances and biomass growth promoters. The colorant initial concentrations and those obtained after biological degradation depending on the contact time, at certain pH values, were established through UV-Vis spectrometry. The studies have shown the measure of possible biological degradation of some organic substances with extended uses, with largely aromatic structure, resistance to biodegradation of microorganisms, commonly used in wastewater treatment plants.

Photo-catalytic Study of Malachite Green Dye Degradation Using Rice Straw Extracted Activated Carbon Supported ZnO Nano-particles

2020 ◽  
Vol 10 (6) ◽  
pp. 849-859
Author(s):  
Radwa A. El-Salamony ◽  
Abeer A. Emam ◽  
Nagwa A. Badawy ◽  
Sara F. El-Morsi
Keyword(s):  
Activated Carbon ◽  
Visible Light ◽  
Rice Straw ◽  
Malachite Green ◽  
Dye Degradation ◽  
Nano Particles ◽  
Oh Radicals ◽  
Impregnation Method ◽  
Photo Degradation ◽  
Malachite Green Dye

Objective: ZnO nanoparticles were synthesized using wet impregnation method, and activated carbon from rice straw (RS) prepared through chemical route. Methods: The nano-composites ZnO-AC series were prepared with different ZnO:AC ratio of 10, 20, 50, and 70% to optimize the zinc oxide nanoparticles used. The obtained composites were characterized by FE-SEM, XRD, SBET, and optical techniques then used for the photo-degradation of Malachite green dye (MG) under visible light. Results: It was found that 10ZnO-AC exhibited excellent visible light photo-catalytic performance. The ·OH radicals’ formation is matching with photo-activity of the prepared composites. The photo-degradation efficiency of MG increased from 63% to 93%, when the 10ZnO-AC photocatalyst amount was increased from 0.5 to 6 g/L. Conclusion: The GC-MS technique was used to analyze the intermediates formed; up to 15 kinds of chemicals were identified as the degradation products.

Effective removal of malachite green dye from aqueous solution in hybrid system utilizing agricultural waste as particle electrodes

Chemosphere ◽  
2021 ◽  
Vol 273 ◽  
pp. 129634
Author(s):  
A. Annam Renita ◽  
Kilaru Harsha Vardhan ◽  
P. Senthil Kumar ◽  
P. Tsopbou Ngueagni ◽  
A. Abilarasu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Malachite Green ◽  
Hybrid System ◽  
Agricultural Waste ◽  
Malachite Green Dye ◽  
Effective Removal

scholarly journals Enhanced photocatalytic efficiency of hydrothermally synthesized g-C3N4/NiO heterostructure for mineralization of malachite green dye

2021 ◽  
Vol 11 ◽  
pp. 970-981
Author(s):  
Suresh Kumar Pandey ◽  
Manish Kumar Tripathi ◽  
V. Ramanathan ◽  
Pradeep Kumar Mishra ◽  
Dhanesh Tiwary
Keyword(s):  
Malachite Green ◽  
Photocatalytic Efficiency ◽  
Malachite Green Dye
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