scholarly journals Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

2021 ◽  
Vol 22 (21) ◽  
pp. 11978
Author(s):  
Dario Paolo ◽  
Franca Locatelli ◽  
Eleonora Cominelli ◽  
Raul Pirona ◽  
Sara Pozzo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Large Scale ◽  
Lignin Content ◽  
Phenylpropanoid Pathway ◽  
Cynara Cardunculus ◽  
Biotechnological Approach ◽  
Candidate Species ◽  
Transgenic Lines ◽  
Set Up

Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefaciens-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the Arabidopsis thaliana MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of AtMYB4 overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

Leveraging the Cloud for Large-Scale Software Testing – A Case Study

2012 ◽  
pp. 252-279 ◽  
Author(s):  
Anjan Pakhira ◽  
Peter Andras
Keyword(s):  
Large Scale ◽  
Analysis Data ◽  
System Level ◽  
Small Scale ◽  
Test Cases ◽  
Software Life Cycle ◽  
Technical Issues ◽  
Wide Scale ◽  
Set Up

Testing is a critical phase in the software life-cycle. While small-scale component-wise testing is done routinely as part of development and maintenance of large-scale software, the system level testing of the whole software is much more problematic due to low level of coverage of potential usage scenarios by test cases and high costs associated with wide-scale testing of large software. Here, the authors investigate the use of cloud computing to facilitate the testing of large-scale software. They discuss the aspects of cloud-based testing and provide an example application of this. They describe the testing of the functional importance of methods of classes in the Google Chrome software. The methods that we test are predicted to be functionally important with respect to a functionality of the software. The authors use network analysis applied to dynamic analysis data generated by the software to make these predictions. They check the validity of these predictions by mutation testing of a large number of mutated variants of the Google Chrome. The chapter provides details of how to set up the testing process on the cloud and discusses relevant technical issues.

Large scale cultivation of Cynara cardunculus L. for biomass production—A case study

2011 ◽  
Vol 33 (1) ◽  
pp. 1-6 ◽  
Author(s):  
J. Gominho ◽  
A. Lourenço ◽  
P. Palma ◽  
M.E. Lourenço ◽  
M.D. Curt ◽  
...  
Keyword(s):  
Biomass Production ◽  
Large Scale ◽  
Cynara Cardunculus ◽  
Large Scale Cultivation

scholarly journals Diagnosis Index System Setup for Implementation Status Management in Large-Scale Construction Projects

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Wan-Yu Wang ◽  
Yi Wang
Keyword(s):  
Rough Set ◽  
Index System ◽  
Construction Projects ◽  
Large Scale ◽  
Mathematic Model ◽  
Scale Construction ◽  
The Index System ◽  
Set Up ◽  
Implementation Status

In order to scientifically set up the diagnosis index system for the implementation state of large-scale construction projects, this paper proposed a new method which takes into account the indicators in all level states. Different from the index system constructed by other methods, the indexes/indicators established in this paper are more systematically correlated, with a better hierarchical progression in all levels of the index system. The particular diagnosis index parameters of the management objects are firstly analysed through the mathematic model based on the Rough Set. Then, the representation of the periodical management problems is taken as the study object, and the detailed establishing process to form the index system is presented based on the evidenced theory and the Rough Set extraction. Finally, a case study is presented to validate the proposed method. It is shown that the index system set up by the proposed method can not only represent the systematic hierarchical relationships among all corresponding indexes but also diagnose the macroscopic, the mesostates, and the microstates effectively.

scholarly journals Evaluation of an OBCE Conversion Contract in a Large-scale Oil and Gas Project

2020 ◽  
Vol 312 ◽  
pp. 02006
Author(s):  
Ruveyda Komurlu ◽  
Akin Er
Keyword(s):  
Large Scale ◽  
Oil And Gas ◽  
Oil And Gas Industry ◽  
Lessons Learned ◽  
Project Schedule ◽  
Gas Industry ◽  
Maximum Benefit ◽  
Set Up ◽  
Distinguishing Features

Billion-dollar investments are quite common in oil and gas industry and owners generally prefer engineering, procurement, construction (EPC) contracts since they would like to minimize their risks and guarantee the most consistent project cost and shortest timeline scheme. Considering the size of contracts, owners are unsurprisingly seeking an ideal tender awarding method to avoid deviation from project schedule or budget and get the maximum benefit for them. They may prefer to award the EPC contract directly (i), have a front-end engineering design (FEED) study done first and award the EPC contract afterwards (ii), or set up a convertible contract and convert it to EPC after an open book cost estimate (OBCE) process (iii). It is a question of concern which option for large-scale oil and gas projects is the most favourable. As a case study, outcomes of a project which was administrated with a conversion type of contract will be reviewed in detail, and a number of principles based on lessons learned will be listed. Thus, the authors aim to provide a verifying approach for the interpretation of EPC conversion type contract management based on analysis of the distinguishing features of large-scale oil and gas projects.

scholarly journals Public Sculpture and Social Practice in the Roman Empire

2021 ◽  
pp. 269-291
Author(s):  
Elizabeth Wolfram Thill
Keyword(s):  
Roman Empire ◽  
Social Practice ◽  
Large Scale ◽  
Public Areas ◽  
Set Up ◽  
History And Myth ◽  
Public Sculpture

One of the more fruitful lines of research in recent decades has been the exploration of how Roman sculpture interacted with the lives of its contemporary viewers. This chapter employs monumental reliefs, large-scale sculptures set up in public areas by official authorities, as a case study to examine how sculpture contributed to social practice under the Roman emperors. Particular focus is given to the phenomenon of imperial portrait types, the blending of history and myth in sculpted narratives, issues of visibility, and the afterlife of some reliefs. The chapter also examines possible means of evaluating responses to relief monuments, from provincial imitations, to private copies in other media, to the written record. In the end, monumental reliefs prove an excellent means to highlight the general dissonance between ancient and modern perceptions of sculpture.

A Family With Autosomal-Dominant Progressive Sensorineural Hearing Loss

1996 ◽  
Vol 5 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Chris Halpin ◽  
Barbara Herrmann ◽  
Margaret Whearty
Keyword(s):  
Speech Production ◽  
Hearing Aids ◽  
Role Models ◽  
Speech Intelligibility ◽  
Large Scale ◽  
Speech Language Pathology ◽  
The Family ◽  
Patient Will ◽  
Language Pathology

The family described in this article provides an unusual opportunity to relate findings from genetic, histological, electrophysiological, psychophysical, and rehabilitative investigation. Although the total number evaluated is large (49), the known, living affected population is smaller (14), and these are spread from age 20 to age 59. As a result, the findings described above are those of a large-scale case study. Clearly, more data will be available through longitudinal study of the individuals documented in the course of this investigation but, given the slow nature of the progression in this disease, such studies will be undertaken after an interval of several years. The general picture presented to the audiologist who must rehabilitate these cases is that of a progressive cochlear degeneration that affects only thresholds at first, and then rapidly diminishes speech intelligibility. The expected result is that, after normal language development, the patient may accept hearing aids well, encouraged by the support of the family. Performance and satisfaction with the hearing aids is good, until the onset of the speech intelligibility loss, at which time the patient will encounter serious difficulties and may reject hearing aids as unhelpful. As the histological and electrophysiological results indicate, however, the eighth nerve remains viable, especially in the younger affected members, and success with cochlear implantation may be expected. Audiologic counseling efforts are aided by the presence of role models and support from the other affected members of the family. Speech-language pathology services were not considered important by the members of this family since their speech production developed normally and has remained very good. Self-correction of speech was supported by hearing aids and cochlear implants (Case 5’s speech production was documented in Perkell, Lane, Svirsky, & Webster, 1992). These patients received genetic counseling and, due to the high penetrance of the disease, exhibited serious concerns regarding future generations and the hope of a cure.

The Boeing 787 Dreamliner--A case study in large-scale design integration

2008 ◽  
Author(s):  
D. L. McMullin ◽  
A. R. Jacobsen ◽  
D. C. Carvan ◽  
R. J. Gardner ◽  
J. A. Goegan ◽  
...  
Keyword(s):  
Large Scale ◽  
Design Integration ◽  
Scale Design

Platelet Anti-Aggregating Activity and Tolerance of Clopidogrel in Atherosclerotic Patients

1996 ◽  
Vol 76 (06) ◽  
pp. 0939-0943 ◽  
Author(s):  
B Boneu ◽  
G Destelle ◽  
Keyword(s):  
Platelet Aggregation ◽  
Large Scale ◽  
Bleeding Time ◽  
Antithrombotic Activity ◽  
Ambulatory Patients ◽  
Patients At Risk ◽  
Set Up ◽  
Ischemic Events ◽  
Large Scale Clinical Trial ◽  
Selection Of

SummaryThe anti-aggregating activity of five rising doses of clopidogrel has been compared to that of ticlopidine in atherosclerotic patients. The aim of this study was to determine the dose of clopidogrel which should be tested in a large scale clinical trial of secondary prevention of ischemic events in patients suffering from vascular manifestations of atherosclerosis [CAPRIE (Clopidogrel vs Aspirin in Patients at Risk of Ischemic Events) trial]. A multicenter study involving 9 haematological laboratories and 29 clinical centers was set up. One hundred and fifty ambulatory patients were randomized into one of the seven following groups: clopidogrel at doses of 10, 25, 50,75 or 100 mg OD, ticlopidine 250 mg BID or placebo. ADP and collagen-induced platelet aggregation tests were performed before starting treatment and after 7 and 28 days. Bleeding time was performed on days 0 and 28. Patients were seen on days 0, 7 and 28 to check the clinical and biological tolerability of the treatment. Clopidogrel exerted a dose-related inhibition of ADP-induced platelet aggregation and bleeding time prolongation. In the presence of ADP (5 \lM) this inhibition ranged between 29% and 44% in comparison to pretreatment values. The bleeding times were prolonged by 1.5 to 1.7 times. These effects were non significantly different from those produced by ticlopidine. The clinical tolerability was good or fair in 97.5% of the patients. No haematological adverse events were recorded. These results allowed the selection of 75 mg once a day to evaluate and compare the antithrombotic activity of clopidogrel to that of aspirin in the CAPRIE trial.

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