scholarly journals Identification of an ATP-Binding Cassette Transporter Implicated in Aluminum Tolerance in Wild Soybean (Glycine soja)

2021 ◽  
Vol 22 (24) ◽  
pp. 13264
Author(s):  
Ke Wen ◽  
Huanting Pan ◽  
Xingang Li ◽  
Rong Huang ◽  
Qibin Ma ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Glycine Soja ◽  
Aluminum Tolerance ◽  
Wild Soybean ◽  
Atp Binding ◽  
Aluminum Stress ◽  
Ion Translocation ◽  
Atp Binding Cassette Transporter ◽  
Transgenic Lines ◽  
Atp Binding Cassette

The toxicity of aluminum (Al) in acidic soil limits global crop yield. The ATP-binding cassette (ABC) transporter-like gene superfamily has functions and structures related to transportation, so it responds to aluminum stress in plants. In this study, one half-size ABC transporter gene was isolated from wild soybeans (Glycine soja) and designated GsABCI1. By real-time qPCR, GsABCI1 was identified as not specifically expressed in tissues. Phenotype identification of the overexpressed transgenic lines showed increased tolerance to aluminum. Furthermore, GsABCI1 transgenic plants exhibited some resistance to aluminum treatment by ion translocation or changing root components. This work on the GsABCI1 identified the molecular function, which provided useful information for understanding the gene function of the ABC family and the development of new aluminum-tolerant soybean germplasm.

scholarly journals ATP Binding Cassette Transporter A1 is Involved in Extracellular Secretion of Acetylated APE1/Ref-1

2019 ◽  
Vol 20 (13) ◽  
pp. 3178 ◽  
Author(s):  
Yu Ran Lee ◽  
Hee Kyoung Joo ◽  
Eun Ok Lee ◽  
Hyun Sil Cho ◽  
Sunga Choi ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Abc Transporters ◽  
Secretory Pathway ◽  
Trichostatin A ◽  
Atp Binding ◽  
Terminal Protein ◽  
Secretion Signal ◽  
Extracellular Secretion ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

Acetylation of nuclear apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) is associated with its extracellular secretion, despite the lack of an N-terminal protein secretion signal. In this study, we investigated plasma membrane targeting and translocation of APE1/Ref-1 in HEK293T cells with enhanced acetylation. While APE1/Ref-1 targeting was not affected by inhibition of the endoplasmic reticulum/Golgi-dependent secretion, its secretion was reduced by inhibitors of ATP-binding cassette (ABC) transporters, and siRNA-mediated down-regulation of ABC transporter A1. The association between APE1/Ref-1 and ABCA1 transporter was confirmed by proximal ligation assay and immunoprecipitation experiments. An APE1/Ref-1 construct with mutated acetylation sites (K6/K7R) showed reduced co-localization with ABC transporter A1. Exposure of trichostatin A (TSA) induced the acetylation of APE1/Ref-1, which translocated into membrane fraction. Taken together, acetylation of APE1/Ref-1 is considered to be necessary for its extracellular targeting via non-classical secretory pathway using the ABCA1 transporter.

Evidence for the presence of ATP-binding cassette transporter (ABC transporter) A1 in stallion spermatozoa

2012 ◽  
Vol 32 (8) ◽  
pp. 497 ◽  
Author(s):  
M. Merkl ◽  
S. Schäfer-Somi ◽  
I. Walter ◽  
M. Helmreich ◽  
M. Glösmann ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

scholarly journals Maltose-binding protein effectively stabilizes the partially closed conformation of the ATP-binding cassette transporter MalFGK2

2017 ◽  
Vol 19 (14) ◽  
pp. 9366-9373 ◽  
Author(s):  
Jingwei Weng ◽  
Shuo Gu ◽  
Xin Gao ◽  
Xuhui Huang ◽  
Wenning Wang
Keyword(s):  
Abc Transporter ◽  
Binding Protein ◽  
Maltose Binding Protein ◽  
Atp Binding ◽  
Type I ◽  
Closed Conformation ◽  
Atp Binding Cassette Transporter ◽  
Maltose Transporter ◽  
Atp Binding Cassette

Maltose transporter MalFGK2is a type-I importer in the ATP-binding cassette (ABC) transporter superfamily.

Repeated Ivermectin Treatment Induces Ivermectin Resistance in Strongyloides ratti by Upregulating the Expression of ATP-Binding Cassette Transporter Genes

2021 ◽  
Author(s):  
Chatchawan Sengthong ◽  
Manachai Yingklang ◽  
Kitti Intuyod ◽  
Ornuma Haonon ◽  
Porntip Pinlaor ◽  
...  
Keyword(s):  
Larval Development ◽  
Abc Transporter ◽  
Resistance Mechanism ◽  
Atp Binding ◽  
Expression Levels ◽  
Atp Binding Cassette Transporter ◽  
Sensitivity Tests ◽  
Abc Transporter Genes ◽  
Atp Binding Cassette

Ivermectin (IVM) is a widely used anthelmintic. However, with widespread use comes the risk of the emergence of IVM resistance, particularly in strongyloidiasis. Adenosine triphosphate (ATP)-binding cassette (ABC) transporter genes play an important role in the IVM-resistance mechanism. Here, we aimed to establish an animal experimental model of IVM resistance by frequent treatment of Strongyloides ratti with subtherapeutic doses of IVM, resistance being evaluated by the expression levels of ABC transporter genes. Rats infected with S. ratti were placed in experimental groups as follows: 1) untreated control (control); 2) treated with the mutagen ethyl methanesulfonate (EMS); 3) injected with 100 µg/kg body weight of IVM (IVM); 4) treated with a combination of EMS and IVM (IVM+EMS). Parasites were evaluated after four generations. Extent of IVM resistance was assessed using IVM sensitivity, larval development, and expression of ABC genes. By the F4 generation, S. ratti in the IVM group exhibited significantly higher levels of IVM resistance than did other groups according to in vitro drug-sensitivity tests and inhibition of larval development (IC50 = 36.60 ng/mL; 95% CI: 31.6, 42.01). Expression levels of ABC isoform genes (ABCA, ABCF, and ABCG) were statistically significantly higher in the IVM-resistant line compared with the susceptible line. In conclusion, IVM subtherapeutic doses induced IVM resistance in S. ratti by the F4 generation with corresponding upregulation of some ABC isoform genes. The study provides a model for inducing and assessing drug resistance in Strongyloides.

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