scholarly journals Spectral Transmission of the Human Corneal Layers

2021 ◽  
Vol 10 (19) ◽  
pp. 4490
Author(s):  
Cristina Peris-Martínez ◽  
Mari Carmen García-Domene ◽  
Mariola Penadés ◽  
María Josefa Luque ◽  
Ester Fernández-López ◽  
...  

We have assessed the spectral transmittance of the different layers of the human cornea in the ultraviolet (UV), visible, and near-infrared (IR) spectral ranges. Seventy-four corneal sample donors were included in the study. Firstly, the corneal transmittance was measured using a spectrophotometer. Then, all samples were fixed for histopathological analysis, which allowed us to measure the thickness of each corneal layer. Finally, the absorption coefficients of the corneal layers were computed by a linear model reproducing total transmittance. The results show that corneal transmission was almost in unity at the visible and IR ranges but not at the UV range, in which the layer with higher transmission is Descemet’s membrane, whereas the stroma showed the lowest transmittance. Regarding the absorption coefficient, the most absorptive tissue was Bowman’s layer, followed by the endothelium. Variations on transmittance due to changes in the stroma, Bowman’s layer, or Descemet layer were simulated, and important transmission increases were found due to stroma and Bowman changes. To conclude, we have developed a method to measure the transmittance and thickness for each corneal layer. All corneal layers absorb UV light to a greater or lesser extent. The absorption coefficient is higher for Bowman’s layer, while the stroma is the layer with the lowest transmittance due to its thickness. Variations in stroma thickness or changes in the corneal tissue of Bowman’s layer or the endothelium layer due to some pathologies or surgeries could affect, to a greater or lesser degree, the total transmission of the cornea. Thus, obtaining accurate absorption coefficients for different layers would help us to predict and compensate these changes.

2013 ◽  
Vol 667 ◽  
pp. 384-387
Author(s):  
Mohamed Zahidi Musa ◽  
Mohamad Hafiz Mamat ◽  
Mohamad Rusop

The electrical properties of nanostructured Aluminum (Al) doped Zinc Oxide (ZnO) thin films based ultraviolet (UV) sensor prepared by sol-gel spin-coating method have been investigated. Uniform nanoparticles Al doped ZnO have been deposited with high absorption coefficient at UV region and low absorption coefficient properties in visible and near-infrared (NIR) region. I-V spectra show high sensitivity characteristic of UV sensor with fast response after UV light exposure.


2003 ◽  
Vol 376 (3) ◽  
pp. 615-623 ◽  
Author(s):  
Aglaia PAPPA ◽  
Tia ESTEY ◽  
Rizwan MANZER ◽  
Donald BROWN ◽  
Vasilis VASILIOU

ALDH3A1 (aldehyde dehydrogenase 3A1) is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UV-light-induced damage. The precise biological function(s) and cellular distribution of ALDH3A1 in the corneal tissue remain to be elucidated. Among the hypotheses proposed for ALDH3A1 function in cornea is detoxification of aldehydes formed during UV-induced lipid peroxidation. To investigate in detail the biochemical properties and distribution of this protein in the human cornea, we expressed human ALDH3A1 in Sf9 insect cells using a baculovirus vector and raised monoclonal antibodies against ALDH3A1. Recombinant ALDH3A1 protein was purified to homogeneity with a single-step affinity chromatography method using 5´-AMP–Sepharose 4B. Human ALDH3A1 demonstrated high substrate specificity for medium-chain (6 carbons and more) saturated and unsaturated aldehydes, including 4-hydroxy-2-nonenal, which are generated by the peroxidation of cellular lipids. Short-chain aliphatic aldehydes, such as acetaldehyde, propionaldehyde and malondialdehyde, were found to be very poor substrates for human ALDH3A1. In addition, ALDH3A1 metabolized glyceraldehyde poorly and did not metabolize glucose 6-phosphate, 6-phosphoglucono-δ-lactone and 6-phosphogluconate at all, suggesting that this enzyme is not involved in either glycolysis or the pentose phosphate pathway. Immunohistochemistry in human corneas, using the monoclonal antibodies described herein, revealed ALDH3A1 expression in epithelial cells and stromal keratocytes, but not in endothelial cells. Overall, these cumulative findings support the metabolic function of ALDH3A1 as a part of a corneal cellular defence mechanism against oxidative damage caused by aldehydic products of lipid peroxidation. Both recombinant human ALDH3A1 and the highly specific monoclonal antibodies described in the present paper may prove to be useful in probing biological functions of this protein in ocular tissue.


Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2036
Author(s):  
Miren Blanco ◽  
Cristina Monteserín ◽  
Nerea Uranga ◽  
Estíbaliz Gómez ◽  
Estíbaliz Aranzabe ◽  
...  

The transport sector is the fastest growing contributor to climate emissions and experiences the highest growth in energy use. This study explores the use of TiO2 nanoparticles for obtaining photocatalytic nanocomposites with improved infrared reflectance properties. The nanocomposites were prepared by dispersing 0–20 wt% of TiO2 nanoparticles in an unsaturated polyester resin. The effect of TiO2 on the curing kinetics was studied by differential scanning calorimetry, showing a significant delay of the curing reactions. The thermal reflectance of the modified resins was characterized by UV-Vis-NIR spectrophotometry, measuring total solar reflectance (TSR). The TiO2 greatly increased the TSR of the resin, due to the reflectance properties of the nanoparticles and the change in color of the modified resin. These nanocomposites reflect a significant part of near-infrared radiation, which can contribute to a reduction of the use of heating, ventilation, and air conditioning. Moreover, the photocatalytic effect of the TiO2 modified nanocomposites was studied by monitoring the degradation of an organic model contaminant in an aqueous medium under UV light, and the reusability of the nanocomposites was studied with 5 cycles. The developed nanocomposites are proposed as a solution for reducing global warming and pollutant emissions.


2014 ◽  
Vol 875-877 ◽  
pp. 467-471
Author(s):  
Ning Wang ◽  
Xiao Xia Li

The electrically controlled birefringence of nematic liquid crystal BL-009 was measured by polarized interference method. The influence of LC absorption effect, the birefringence variation, is discussed in this paper. The experiments results showed the influence to birefringence is big in infrared region. Not only the birefringence value is greatly different with that of unconsidering absorption effect, but also the gradient changing of birefringence curves is obvious. Furthermore, the electrically controlled birefringences of two conditions are compared when the absorption coefficients of ordinary light and the extraordinary light are nearly same and greatly different. The analysis demonstrated the approximate method of absorption coefficient is feasible.


Author(s):  
Li Jiang ◽  
Huihuang Huang ◽  
Fubing Gui ◽  
Yalun Xu ◽  
Qianqian Lin

Recent years, AgBiS2 has received great attention in optoelectronics thanks to the fascinated merits, including suitable bandgap, large absorption coefficient from ultra-violet to near-infrared and remarkable material stability. However, the...


2017 ◽  
Author(s):  
K. Max Zhang ◽  
Bo Yang ◽  
Geng Chen ◽  
Jiajun Gu ◽  
James Schwab ◽  
...  

Abstract. DC, also referred to as Delta-C, measures enhanced light absorption of particulate matter (PM) samples at the near-ultraviolet (UV) range relative to the near-infrared range, which has been proposed previously as a woodsmoke marker due to the presence of enhanced UV light absorbing materials from wood combustion. In this paper, we further evaluated the applications and limitations of using DC as both a qualitative and semi-quantitative woodsmoke marker via joint continuous measurements of PM2.5 (by nephelometer pDR-1500) and light-absorptive PM (by 2-wavelength and 7-wavelength Aethalometer®) in three Northeastern U.S. cities/towns including Rutland, VT, Saranac Lake, NY and Ithaca, NY. We compared the pDR-1500 against a FEM PM2.5 sampler (BAM 1020), and identified a close agreement between the two instruments in a woodsmoke-dominated ambient environment. The analysis of seasonal and diurnal trends of DC, BC (880 nm) and PM2.5 concentrations supports the use of DC as an adequate qualitative marker. The strong linear relationships between PM2.5 and DC in both woodsmoke-dominated ambient and plume environments suggest that DC can reasonably serve as a semi-quantitative woodsmoke marker. We proposed a DC-based indicator for woodsmoke emission, which was then shown to exhibit relatively strong linear relationship with heating demand. While we observed reproducible PM2.5-DC relationships in similar woodsmoke-dominated ambient environments, those relationships differ significantly with different environments, and among individual woodsmoke sources. DC correlated much more closely with PM2.5 than EcoChem PAS2000-reported PAH in woodsmoke-dominated ambient environments. Our analysis also indicates the potential for PM2.5-DC relationships to be utilized to distinguish different combustion and operating conditions of woodsmoke sources, and that DC-Heating demand relationships could be adopted to estimate woodsmoke emissions. However, future studies are needed to elucidate those relationships.


1989 ◽  
Vol 43 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Dane Bićanić ◽  
Siegfried Krüger ◽  
Paul Torfs ◽  
Bruno Bein ◽  
Frans Harren

An experimental setup for performance of reverse mirage spectroscopy at CO2 laser wavelengths on liquid samples having high values of absorption coefficients is described. One and the same liquid is used as both the absorbing and deflecting medium. The Rosencwaig-Gersho theory has been applied, and the choice of experimental conditions that would enable determination of absorption coefficient β from the magnitude of photothermal signals measured at two different probe beam distances (probing locations) is discussed. The usefulness of this technique (essentially not inhibited by the requirements imposed on the sample's thickness) is tested on methanol having absorption coefficients β close to 300 cm−1 in the wavelength region covered by CO2 laser emission.


1975 ◽  
Vol 19 ◽  
pp. 381-391 ◽  
Author(s):  
F. Bazan ◽  
N. A. Bonner

The discovery of a very simple and useful relationship between the absorption coefficient of a particular element and the ratio of incoherent to coherent scattering by the sample containing the element is discussed. By measuring the absorption coefficients for a few elements in a few samples, absorption coefficients for many elements in an entire set of similar samples can be obtained.


2021 ◽  
Vol 263 (1) ◽  
pp. 5571-5577
Author(s):  
Reiji Tomiku ◽  
Noriko Okamoto ◽  
Toru Otsuru ◽  
Shun Iwamoto ◽  
Shoma Suzuki

The absorption coefficients in a reverberation room are most representative measure for evaluating absorption performance of architectural materials. However, it is well known that measurement results of the coefficient vary according to a room shape of the measurement and area of the specimen. Numerical analyses based on wave acoustics are effective tools to investigate these factors on absorption coefficient measurement in reverberation room. In this study, sound fields for the measurement of absorption coefficient in reverberation room are analyzed by time domain finite element method (TDFEM). This study shows effectiveness of the analysis for investigation on causes of variation in the measurement results and improvement methods of the measurement. First, some measurement sound fields for absorption coefficient in reverberation rooms the walls of which are incline or decline are analyzed by the TDFEM. Next, reverberation times in each sound fields are calculated from the results obtained by TDFEM and the absorption coefficients are evaluated from the reverberation time of the room with and without specimen. Finally, the relationships among room shape, degree of inclination of the wall, the sound absorption coefficient of the specimen, frequencies and the measurement absorption coefficient are investigated.


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