scholarly journals The Association between Bisphenol A, Steroid Hormones, and Selected MicroRNAs Levels in Seminal Plasma of Men with Infertility

2021 ◽  
Vol 10 (24) ◽  
pp. 5945
Author(s):  
Ewelina Palak ◽  
Weronika Lebiedzińska ◽  
Sławomir Anisimowicz ◽  
Maria Sztachelska ◽  
Piotr Pierzyński ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Circulating Mirnas ◽  
Endocrine Disrupting Chemical ◽  
Endocrine Disrupting ◽  
Healthy Control ◽  
Mirnas Expression ◽  
Semen Morphology

Bisphenol A (BPA), the most common endocrine-disrupting chemical, has been associated with male reproductive dysfunctions. Recently, it has been shown that BPA may also affect miRNAs expression. Herein, we aimed to evaluate the association of BPA levels with steroid hormone concentration and circulating miRNAs levels to investigate the potential direct effect of BPA on homeostasis in the testis environment. The level of BPA in the seminal plasma of azoospermic men was significantly higher compared to the healthy control. The concentrations of estradiol (E2) and androstenedione (A) were significantly decreased in the seminal plasma of azoospermic men compared to the normospermic men. The levels of miR-let-7a, miR-let-7b, and miR-let-7c were significantly up-regulated, and the level of miR-518f was significantly down-regulated in the seminal plasma of the azoospermic men compared to the healthy control. The level of BPA correlated negatively with sperm concentration and normal semen morphology. A significant positive correlation was found between BPA levels and miR-let-7a and miR-let-7c levels, whereas BPA negatively correlated with miR-518f levels. Our results suggest that BPA may negatively affect sperm quality. Moreover, BPA correlated with the miR-let-7a, miR-let-7c, and miR-518f levels in seminal plasma, which suggests that BPA may act directly in seminal plasma, affecting the testicular environment.

scholarly journals Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

Micromachines ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 922
Author(s):  
Dong Lee ◽  
Moo-Yeal Lee ◽  
Sukkil Koh ◽  
Mihi Yang
Keyword(s):  
Bisphenol A ◽  
Liver Cells ◽  
Pyrus Pyrifolia ◽  
Endocrine Disrupting Chemical ◽  
Protein Levels ◽  
Ic50 Value ◽  
Endocrine Disrupting ◽  
Cultured Liver Cells ◽  
Ic50 Values ◽  
Hep3b Cells

A micropillar/microwell chip platform with 3D cultured liver cells has been used for HTP screening of hepatotoxicity of bisphenol A (BPA), an endocrine-disrupting chemical. We previously found the hepatotoxicity of BPA is alleviated by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase 2 (ALDH2). In this study, we have tested potential BPA detoxification with Korean pear (Pyrus pyrifolia) extract, stimulators of ADH and ALDH, as well as arbutin, a reference compound in the pears, on the micropillar/microwell chip platform with human liver cells. Surprisingly, the toxicity of BPA was reduced in the presence of Korean pear extract, indicated by significantly increased IC50 values. The IC50 value of BPA with Korean pear extract tested against HepG2 cells was shifted from 151 to 451 μM, whereas those tested against Hep3B cells was shifted from 110 to 204 μM. Among the tested various concentrations, 1.25, 2.5, and 5 mg/mL of the extract significantly reduced BPA toxicity (Ps < 0.05). However, there was no such detoxification effects with arbutin. This result was supported by changes in protein levels of ADH in the liver cells.

Modulation of cytokine expression in human macrophages by endocrine-disrupting chemical Bisphenol-A

2014 ◽  
Vol 451 (4) ◽  
pp. 592-598 ◽  
Author(s):  
Yanzhen Liu ◽  
Chenfang Mei ◽  
Hao Liu ◽  
Hongsheng Wang ◽  
Guoqu Zeng ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cytokine Expression ◽  
Endocrine Disrupting Chemical ◽  
Human Macrophages ◽  
Endocrine Disrupting

scholarly journals Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lorena Padilla ◽  
Marina López-Arjona ◽  
Silvia Martinez-Subiela ◽  
Heriberto Rodriguez-Martinez ◽  
Jordi Roca ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Reproductive Function ◽  
Peptide Hormone ◽  
Large White ◽  
Male Reproductive Function ◽  
Farrowing Rate

Abstract Background Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry. Concentrations of the small peptide hormone oxytocin (OXT), involved in male reproductive function and present in the seminal plasma (SP) of several species could be a robust one. This study characterized concentrations of SP-OXT in ejaculates from boars used in artificial insemination (AI) programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored AI-semen. Seminal OXT concentrations (ng/mL) were measured in 169 ejaculates from 61 boars of the Duroc, Pietrain, Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA® technology. Ejaculate (ejaculate volume, sperm concentration, total sperm count) and sperm parameters (motility, viability, intracellular generation of reactive oxygen species, plasma membrane fluidity) were assessed at 0 h and 72 h in AI-semen samples stored at 17 °C. In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated > 100 sows and evaluated both farrowing rate and litter size of 3,167 sows. Results The results showed that SP-OXT differed between boars and between ejaculates within boar (P < 0.05) but not between breeds (Duroc, Pietrain, Landrace and Large White). Ejaculates with higher SP-OXT concentration/mL (hierarchically grouped; P < 0.001) had larger volume and came from younger boars (P < 0.05). Ejaculates of boars showing positive farrowing rate deviation exhibited higher (P < 0.05) SP-OXT concentration/mL than those with negative farrowing rate deviation. Conclusion The SP concentrations of OXT are boar, ejaculate and age dependent, and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

scholarly journals Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study

2021 ◽  
Author(s):  
Aylin Jamali Khaghani ◽  
Parisa Farrokh ◽  
Saeed Zavareh
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Promoter Methylation ◽  
Inhibitory Effect ◽  
Promoter Regions ◽  
Endocrine Disrupting Chemical ◽  
Epigenetic Effects ◽  
Endocrine Disrupting ◽  
The Impact

Background: Bisphenol A (BPA), a synthetic endocrine-disrupting chemical, is a reproductive toxicant. Granulosa cells have significant roles in follicle development, and KIT ligand (KITL) and Anti-Müllerian hormone (AMH) are essential biomolecules produced by them during folliculogenesis. Objective: Due to the widespread use of BPA and its potential epigenetic effects, this study examined the impact of BPA on promoter methylation of amh and kitl genes in mouse granulosa cells. Materials and Methods: Preantral follicles were isolated from ovaries of immature mice and cultured for eight days. Then, follicles were treated with 50 and 100 μM of BPA, and 0.01% (v/v) ethanol for 24 and 72 hr. Growth and degeneration of follicles and antrum formation were analyzed. The granulosa cells were isolated mechanically, and their extracted DNA was treated with sodium bisulfite. The promoter regions of the amh and kitl were analyzed with PCR and sequencing. Results: BPA did not change follicle survival and antrum formation significantly (p = 0.41). However, the culture in the presence of 100 μM BPA had an inhibitory effect on growth. Before BPA treatment, the CpG of the kitl and amh promoters were unmethylated and partially methylated, respectively. While the percent of 5mC in the amh promoter reduced at 100 μM of BPA, it did not alter the kitl promoter methylation. Conclusion: BPA at higher concentrations has an inhibitory effect on follicle growth. Moreover, it seems that the epigenetic impact of BPA restricts to the demethylation of CpG sites. Key words: Bisphenol A, DNA methylation, Granulosa cells.

Bisphenol A Exposure Disrupts Enamel Formation via EZH2-Mediated H3K27me3

2021 ◽  
pp. 002203452199579
Author(s):  
H. Li ◽  
D. Cui ◽  
L. Zheng ◽  
Y. Zhou ◽  
L. Gan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Progenitor Cells ◽  
Enamel Formation ◽  
Developmental Defects ◽  
Endocrine Disrupting Chemical ◽  
Complex Biological Process ◽  
Endocrine Disrupting ◽  
Repressive Histone Modification ◽  
Chromatin Immunoprecipitation Sequencing ◽  
Excessive Proliferation

Enamel formation is a serial and complex biological process, during which related genes are expressed progressively in a spatiotemporal manner. This process is vulnerable to environmental cues, resulting in developmental defects of enamel (DDE). However, how environmental factors are biologically integrated during enamel formation is still poorly understood. Here, we investigated the mechanism of DDE elicited by a model endocrine-disrupting chemical, bisphenol A (BPA), in mouse incisors. We show that BPA exposure leads to DDE in mouse incisors, as well as excessive proliferation in dental epithelial stem/progenitor cells. Western blotting, chromatin immunoprecipitation sequencing, and immunofluorescence staining revealed that this effect was accompanied by upregulation of a repressive mark, H3K27me3, in the labial cervical loop of mouse incisors. Perturbation of H3K27me3 methyltransferase EZH2 repressed the level of H3K27me3 and partially attenuated the excessive proliferation in dental epithelial stem/progenitor cells and DDE induced by BPA exposure. Overall, our results demonstrate the essential role of repressive histone modification H3K27me3 in DDE elicited by exposure to an endocrine-disrupting chemical.

Migration Study of Bisphenol A from Tea Bottle of Polycarbonate Plastic to Tea Water

2013 ◽  
Vol 821-822 ◽  
pp. 929-932 ◽  
Author(s):  
Xue Hui Cao ◽  
Shu Juan Ji
Keyword(s):  
Bisphenol A ◽  
Epoxy Resins ◽  
High Performance ◽  
Black Tea ◽  
Heating Time ◽  
High Concentration ◽  
Endocrine Disrupting Chemical ◽  
Jasmine Tea ◽  
Migration Study ◽  
Endocrine Disrupting

Bisphenol A (BPA) is an important organic intermediate that is an estrogen and an environmental endocrine disrupting chemical. Bisphenol A is used widely in the production of consumer goods, polycarbonate plastics, epoxy resins. A large amount of studies have indicated that BPA might affect human health. In this work, four different tea (Tieguanyin tea, Black tea, Maofeng tea, Jasmine tea) were tested to verify if they were able to increase the BPA release from the PC cup. High performance liquid chromatography (HPLC) with fluorescence detection (FLD) was used to quantify BPA. Result showed that the heating time and temperature could have an influence on BPA migration from polycarbonate plastic. BPA migrated into tea water from polycarbonate plastic, especially when heated to high temperatures. Relatively high levels of BPA were found to be released into food simulants water from high concentration tea water.

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