scholarly journals Hormone Receptor Expression Variations in Normal Breast Tissue: Preliminary Results of a Prospective Observational Study

2021 ◽  
Vol 11 (5) ◽  
pp. 387
Author(s):  
Giacomo Santandrea ◽  
Chiara Bellarosa ◽  
Dino Gibertoni ◽  
Maria C. Cucchi ◽  
Alejandro M. Sanchez ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Receptor Expression ◽  
Hormonal Stimulation ◽  
Tissue Samples ◽  
Hormonal Receptor ◽  
Hormone Receptor Expression ◽  
Cystic Disease Fluid Protein

Normal breast tissue undergoes great variations during a woman’s life as a consequence of the different hormonal stimulation. The purpose of the present study was to examine the hormonal receptor expression variations according to age, menstrual cycle, menopausal state and body mass index. To this purpose, 49 tissue samples of normal breast tissue, obtained during surgery performed for benign and malignant conditions, were immunostained with Estrogen (ER), Progesterone (PR) and Androgen receptors (AR). In addition, Ki67 and Gross Cystic Disease Fluid Protein were studied. The data obtained revealed a great variability of hormone receptor expression. ER and AR generally increased in older and post-menopausal women, while young women presented a higher proliferative rate, evaluated with Ki67. PR increase was observed in women with BMI higher than 25. The different hormonal receptor expression could favor the development of breast cancer.

scholarly journals Association between cadmium and breast cancer

Medicina ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 415 ◽  
Author(s):  
Loreta Strumylaitė ◽  
Algirdas Boguševičius ◽  
Stanislovas Ryselis ◽  
Darius Pranys ◽  
Lina Poškienė ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Tissue ◽  
Benign Tumor ◽  
Normal Breast Tissue ◽  
Cadmium Concentration ◽  
Normal Breast ◽  
Tissue Samples ◽  
Malignant Breast Tissue ◽  
Two Samples ◽  
Malignant Breast

Cadmium is a known human lung carcinogen, although some studies indicate a link between cadmium exposure and human breast cancer. The objective of this study was to assess cadmium concentration in breast tissue samples of patients with breast cancer and benign breast tumor. Material and methods. The concentration of cadmium was determined in breast tissue samples of 21 breast cancer and 19 benign tumor patients. Two samples of breast tissue from each patient, i.e. tumor and normal tissue close to tumor, were taken for the analysis. Cadmium was determined by atomic absorption spectrometry (Perkin-Elmer, Zeeman 3030). Results. In patients with breast cancer, the mean cadmium concentration was 33.1 ng/g (95% CI, 21.9– 44.4) in malignant breast tissue and 10.4 ng/g (95% CI, 5.6–15.2) in normal breast tissue (P=0.002). In patients with benign tumor, the corresponding values were 17.5 ng/g (95% CI, 8.4–26.5) and 11.8 ng/g (95% CI, 5.1– 18.5) (P=0.3144). There was a statistically significant difference in cadmium concentration between malignant and benign breast tissues (P=0.009). Conclusion. The data obtained show that cadmium concentration is significantly higher in malignant breast tissue as compared with normal breast tissue of the same women or benign breast tissue. Further studies are necessary to determine the association between cadmium concentration in malignant breast tissue and estrogen receptor level, and smoking.

scholarly journals N-myristoyltransferase proteins in breast cancer: prognostic relevance and validation as a new drug target

2021 ◽  
Author(s):  
John R. Mackey ◽  
Justine Lai ◽  
Utkarsh Chauhan ◽  
Erwan Beauchamp ◽  
Wei-Feng Dong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hormone Receptor ◽  
Cancer Biology ◽  
Normal Breast ◽  
Receptor Expression ◽  
Histologic Grade ◽  
Tumor Growth Inhibition ◽  
Epithelial Tissue ◽  
Breast Epithelial ◽  
Hormone Receptor Expression

Abstract Purpose N-myristoyltransferases 1 and 2 (NMT1 and NMT2) catalyze the addition of 14-carbon fatty acids to the N-terminus of proteins. Myristoylation regulates numerous membrane-bound signal transduction pathways important in cancer biology and the pan-NMT inhibitor PCLX-001 is approaching clinical development as a cancer therapy. The tissue distribution, relative abundances, and prognostic value of the two human NMTs remain poorly understood. Methods We generated and validated mutually exclusive monoclonal antibodies (mAbs) specific to human NMT1 and NMT2. These mAbs were used to perform immunohistochemical analysis of the abundance and distribution of NMT1 and NMT2 in normal breast epithelial samples and a large cohort of primary breast adenocarcinomas from the BCIRG001 clinical trial (n = 706). Results NMT1 protein was readily quantified in normal and most transformed breast epithelial tissue and was associated with higher overall histologic grade, higher Ki67, and lower hormone receptor expression. While NMT2 protein was readily detected in normal breast epithelial tissue, it was undetectable in the majority of breast cancers. Detectable NMT2 protein correlated with significantly poorer overall survival (hazard ratio 1.36; P = 0.029) and worse biological features including younger age, higher histologic grade, lower hormone receptor expression, higher Ki67, and p53 positivity. Treatment of cultured breast cancer cells with PCLX-001 reduced cell viability in vitro. Daily oral administration of PCLX-001 to immunodeficient mice bearing human MDA-MB-231 breast cancer xenografts produced significant dose-dependent tumor growth inhibition in vivo. Conclusions These results support further evaluation of NMT immunohistochemistry for patient selection and clinical trials of NMT inhibition in breast cancer patients.

scholarly journals Androgen receptor expression in normal breast tissue and subsequent breast cancer risk

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Kevin H. Kensler ◽  
Francisco Beca ◽  
Gabrielle M. Baker ◽  
Yujing J. Heng ◽  
Andrew H. Beck ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Androgen Receptor ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Receptor Expression ◽  
Androgen Receptor Expression

scholarly journals VIRUS-ASSOCIATED BREAST CANCER (REVIEW AND METAANALYSIS)

2020 ◽  
Vol 64 (1) ◽  
pp. 15-27
Author(s):  
M. Ibragimova ◽  
M. Tsyganov ◽  
L. Pisareva ◽  
N. Litvyakov
Keyword(s):  
Breast Cancer ◽  
Relative Risk ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Meta Analysis ◽  
Hpv Infection ◽  
Normal Breast ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Tissue Samples

The assessment of the etiological role of viruses in the development of breast cancer remains the subject of intense study. This review examines the data on the presence/absence of viral infection in breast cancer tumors and its clinical significance. The reports on the association of breast cancer with three groups of viruses: HMTV (human Mammary Tumor Virus / MMTV-like), Epstein-Barr virus (EBV) and human papillomavirus (HPV) were analyzed. The authors carried out meta-analysis for each type of virus, which demonstrated the association of the viral infection with breast cancer tissue. A meta-analysis of 1389 breast cancer tissue samples and 750 normal breast tissue samples showed a high level of HMTV infection in the breast cancer tissue (30.7%). The relative risk of breast cancer associated with HMTV infection was 16.7 (95% CI: 7.0-39.7, p = 1.69x10-10). For EBV, the meta-analysis of 1131 breast tumor samples and 185 normal breast tissue samples (based on 9 primary studies) showed that the incidence of EBV infection was 30. .4% in tumor breast tissue and 4.3% in normal breast tissue. The relative risk of EBV-associated breast cancer was 3.3 (95% CI: 1.8-5.8, р=0.00006). The meta-analysis of HPV infection included 29 primary studies with 2,446 tumor tissue samples and 1,144 normal tissue samples. The prevalence of HPV in breast cancer samples and in normal tissue samples was 25% and 4.5%, respectively. The relative risk of breast cancer associated with HPV infection was 3.6 (95% CI: 2.3-5.6, p = 2.8x10-8). The data obtained indicate that the studies on the etiological role of HMTV, EBV and HPV in the development of breast cancer are promising.

scholarly journals Immunometric Assays of Ras and c-erbB-2/Neu Overexpression in Breast Cancer: A Pilot Study

1999 ◽  
Vol 14 (3) ◽  
pp. 178-185
Author(s):  
D. Pelizzola ◽  
R. Malagutti ◽  
G. Giovannini ◽  
M. Indelli ◽  
P. Carcoforo ◽  
...  
Keyword(s):  
Overall Survival ◽  
Breast Tissue ◽  
Radioligand Binding ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Breast Carcinomas ◽  
Tissue Samples ◽  
Time To Recurrence ◽  
Erbb2 Expression ◽  
The Relationship

The expression of the ras and c-erbB2 oncoproteins (p21 and p185, respectively), together with estrogen receptor (ER) and progesterone receptor (PgR) determination, has been retrospectively analyzed in 68 primary breast carcinomas and in 19 normal breast tissue samples. The aims of this study were: a) to explore the association between ras and c-erbB2 expression; b) to evaluate the relationship between ras and c-erbB2 expression and both steroid receptor status and the classical clinical and pathological parameters; and c) to compare two different methods for p185 determination. p185 and p21 were measured by enzyme immunoassay (EIA); p185 was also determined by Western blotting (WB); ER and PgR were assayed by radioligand binding assay. The highest value of p185 in benign breast lesions was used as the threshold to distinguish between positive and negative samples. With this threshold the c-erbB2 oncoprotein was overexpressed in 41.2% (with EIA) and in 50% (with WB) of cancer samples. The concordance rate between the two methods was 79.4. No significant association was found between p21 and p185 levels either in cancer or in normal breast tissue samples. Increasing levels of tumor p21 were associated with a shorter time to recurrence and overall survival. Increasing levels of p185 were associated with a significantly shorter time to recurrence (p185 EIA: p=0.04, p185 WB: p=0.029) and overall survival (p185 EIA: p=0.04, p185 WB: p=0.029).

scholarly journals Identification of Novel CircRNA-miRNA-mRNA Regulatory Network and Its Prognostic Prediction in Breast Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Rui Huang ◽  
Hao Yu ◽  
Xiao Zhong
Keyword(s):  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Expression Profiles ◽  
Normal Breast ◽  
Hub Genes ◽  
Tissue Samples ◽  
Expression Levels ◽  
Qpcr Analysis ◽  
Prognostic Prediction ◽  
Breast Tissues

Aim. This study aimed to investigate the expression profiles of circRNAs and candidate circRNA-miRNA-mRNA network in BC. Methods. Differentially expressed circRNAs, miRNAs, and mRNAs (DEcircRNAs, DEmiRNAs, and DEmRNAs) between BC and normal breast tissue samples were screened by analyzing raw data of the RNA sequencing profile. The expression levels of hub genes in 48 pairs of cancerous and tumor-free breast tissues surgically resected from BC patients were determined by RT-qPCR analysis. Results. A total of 145 DEcircRNAs, 140 DEmiRNAs, and 2451 DEmRNAs between BC and normal breast tissue samples were screened out. There were 5 pairs of upcircRNA-downmiRNA-upmRNA network and 20 pairs of downcircRNA-upmiRNA-downmRNA network. EIF4EBP1, DUSP1, EGR2, EZH1, and CBX7 were found to be correlated with overall survival of the patients with BC. The expression level of EIF4EBP1 was increased and the expression levels of DUSP1, EGR2, EZH1, and CBX7 were decreased in cancerous breast tissues compared to tumor-free breast tissues p < 0.0001 . The RT-qPCR results from 48 BC patients were consistent with the bioinformatics results. Conclusion. This study provides a novel perspective to study circRNA-miRNA-mRNA network in BC and assists in the identification of new potential biomarkers to be used for diagnostic and prognostic purposes.

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