scholarly journals Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 371 ◽  
Author(s):  
Myung-Jin Lee ◽  
Min-Ji Kim ◽  
Sang-Hwan Oh ◽  
Jae-Sung Kwon
Keyword(s):  
Candida Albicans ◽  
Flexural Strength ◽  
Methyl Methacrylate ◽  
Cell Viability ◽  
Mtt Assay ◽  
Biological Properties ◽  
Basic Material ◽  
Pathogenic Yeast ◽  
Poly Methyl Methacrylate ◽  
Biofilm Thickness

Despite the many advantages of poly (methyl methacrylate) (PMMA) as a dental polymer, its antifungal and antibacterial effects remain limited. Here, phytoncide was incorporated into PMMA to inhibit fungal and biofilm accumulation without impairing the basic and biological properties of PMMA. A variable amount of phytoncide (0 wt % to 5 wt %) was incorporated into PMMA, and the basic material properties of microhardness, flexural strength and gloss were evaluated. In addition, cell viability was confirmed by MTT assay. This MTT assay measures cell viability via metabolic activity, and the color intensity of the formazan correlates viable cells. The fungal adhesion and viability on the PMMA surfaces were evaluated using Candida albicans (a pathogenic yeast). Finally, the thickness of saliva-derived biofilm was estimated. The flexural strength of PMMA decreased with increasing phytoncide contents, whereas there were no significant differences in the microhardness and gloss (p > 0.05) and the cell viability (p > 0.05) between the control and the phytoncide-incorporated PMMA samples. The amounts of adherent Candida albicans colony-forming unit (CFU) counts, and saliva-derived biofilm thickness were significantly lower in the phytoncide-incorporated PMMA compared to the control (p < 0.05). Hence, it was concluded that the incorporation of appropriate amounts of phytoncide in PMMA demonstrated antifungal effects while maintaining the properties, which could be a possible use in dentistry application such as denture base resin.

scholarly journals A New Form of Intraoral Delivery of Antifungal Drugs for the Treatment of Denture-Induced Oral Candidosis

2009 ◽  
Vol 03 (04) ◽  
pp. 257-266 ◽  
Author(s):  
Wala M. Amin ◽  
Muna H. Al-Ali ◽  
Nesreen A. Salim ◽  
Sandra K. Al-Tarawneh
Keyword(s):  
Candida Albicans ◽  
Methyl Methacrylate ◽  
Fungal Infections ◽  
Ultra Violet ◽  
Antifungal Drugs ◽  
Distilled Water ◽  
Clinical Value ◽  
Sustained Drug Release ◽  
Poly Methyl Methacrylate ◽  
Pmma Resin

ABSTRACTObjectives: To monitor the release of the antifungal drugs Fluconazole, Chlorhexidine and a combination of the two from an auto-polymerized poly (methyl methacrylate) (PMMA) denture base resin; and to investigate the effect of the released drugs upon the growth of Candida albicans.Methods: A high performance liquid chromatography-Ultra violet (HPLC-UV) method was used in the analysis of the released drugs into distilled water from PMMA discs doped with the antifungal drugs Fluconazole (10%), Chlorhexidine (10%) and a combination of the two drugs (5% each). The antifungal efficacy of the released drugs was monitored, microbiologically, employing “well” technique on a Saborauds culture medium inoculated with a resistant strain of Candida albicans.Results: It was shown that Fluconazole, Chlorhexidine and the combination of the two drugs can be successfully incorporated with PMMA. It was found that the drugs leach steadily out of the PMMA resin into distilled water at mouth temperature and that sustained drug release continued throughout the 28 days test period. It was also shown that the released drugs demonstrated an antifungal activity against the resistant Candida albicans and this was most remarkable in the combined drugs samples.Conclusions: The findings of this investigation have a clinical value in terms of their significant contribution to the treatment of fungal infections of the oral cavity. The sustained release of antifungal drugs from the PMMA resin clearly constitutes a new dosage form of these drugs via the poly (methyl methacrylate) delivery system. (Eur J Dent 2009;3:257-266)

scholarly journals Effect of Antibacterial Silver-Releasing Filler on the Physicochemical Properties of Poly(Methyl Methacrylate) Denture Base Material

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4146 ◽  
Author(s):  
Grzegorz Chladek ◽  
Katarzyna Pakieła ◽  
Wojciech Pakieła ◽  
Jarosław Żmudzki ◽  
Marcin Adamiak ◽  
...  
Keyword(s):  
Impact Strength ◽  
Flexural Strength ◽  
Methyl Methacrylate ◽  
Flexural Modulus ◽  
Base Material ◽  
Distilled Water ◽  
Denture Base ◽  
Inorganic Particles ◽  
Poly Methyl Methacrylate ◽  
Enhanced Solubility

Colonization of polymeric dental prosthetic materials by yeast-like fungi and the association of these microorganisms with complications occurring during prosthetic treatment are important clinical problems. In previously presented research, submicron inorganic particles of silver sodium hydrogen zirconium phosphate (S–P) were introduced into poly(methyl methacrylate) (PMMA) denture base material which allowed for obtaining the antimicrobial effect during a 90 day experiment. The aim of the present study was to investigate the flexural strength, impact strength, hardness, wear resistance, sorption, and solubility during three months of storage in distilled water. With increasing S–P concentration after 2 days of conditioning in distilled water, reduced values of flexural strength (107–72 MPa), impact strength (18.4–5.5 MPa) as well as enhanced solubility (0.95–1.49 µg/mm3) were registered, but they were at acceptable levels, and the sorption was stable. Favorable changes included increased hardness (198–238 MPa), flexural modulus (2.9–3.3 GPa), and decreased volume loss during wear test (2.9–0.2 mm3). The percentage changes of the analyzed properties during the 90 days of storage in distilled water were similar for all materials.

scholarly journals Biological Properties of an Heparin-Poly (Methyl Methacrylate) Copolymer

1977 ◽  
Author(s):  
D. Labarre ◽  
G.A. Boffa ◽  
M. Jozefowicz ◽  
M.C. Boffa
Keyword(s):  
Aqueous Solution ◽  
Methyl Methacrylate ◽  
Antithrombin Iii ◽  
Biological Properties ◽  
Anticoagulant Effect ◽  
Factor V ◽  
Clotting Factors ◽  
Poly Methyl Methacrylate ◽  
Methacrylate Copolymer ◽  
Ig G

The heparin-poly(methyl methacrylate) copolymer was prepared by polymerization of methyl methacrylate on an heparin radical. It was initiated by cerium IV ions and heparin in nitric aqueous solution.An heparin effect was present in the plasma in contact with the copolymer. It was not due to a release of heparin in the plasma, but to a contact effect. The anticoagulant effect was depending upon the concentration of the copolymer. The activity of clotting factors in plasma, after a contact with the copolymer was not decreased except for factor V. Antithrombin III was selectively adsorbed on the surface of the copolymer.The copolymer lost progressively its anticoagulant properties after each contact with the plasma. This might be due to the adsorption of fibrinogen on the copolymer. Labelled 125I albumin, transferrin, Ig G and fibrinogen were not similarly desorbed in the presence of serum and of plasma.This copolymer seems to offer interesting properties for blood devices.

The effect of TiO2 and SiO2 nanoparticles on flexural strength of poly (methyl methacrylate) acrylic resins

2013 ◽  
Vol 57 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Ahmad Sodagar ◽  
Abbas Bahador ◽  
Safia Khalil ◽  
Atefe Saffar Shahroudi ◽  
Mohammad Zaman Kassaee
Keyword(s):  
Flexural Strength ◽  
Methyl Methacrylate ◽  
Sio2 Nanoparticles ◽  
Poly Methyl Methacrylate ◽  
Acrylic Resins

scholarly journals TOXICITY TEST OF SMALL WHITE GINGER EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO

2020 ◽  
Vol 5 (1) ◽  
pp. 6
Author(s):  
Robiansyah Robiansyah ◽  
Debby Saputera ◽  
Rahmad Arifin
Keyword(s):  
Candida Albicans ◽  
Cell Viability ◽  
Mtt Assay ◽  
Zingiber Officinale ◽  
Assay Method ◽  
Fibroblast Cells ◽  
Ginger Extract ◽  
Denture Stomatitis ◽  
Alternative Ingredients

Background: Denture stomatitis is inflammation of the oral mucosa which supporting the denture that caused by Candida albicans. Candida albicans contamination can be prevented by immersing dentures into denture cleanser solution. One of the alternative ingredients that can be used as denture cleanser is small white ginger rhizome (Zingiber officinale var. Amarum). Objective: This study aimed to analyze whether small white ginger extract (Zingiber officinale var. Amarum) was toxic to BHK-21 fibroblast cells using the MTT assay method. Method: This study was conducted in 7 groups. Five groups consisted of extracts of 30%, 40%, 50%, 60%, 70% and 2 control groups comprised of media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells of all groups of small white ginger extract treatment was 100%. The parametric analysis of One Way Annova showed p = 0.498 (p> 0.05) Conclusion: Small white ginger extract (Zingiber officinale var. Amarum) is not toxic to BHK-21 fibroblast cells using the MTT Assay method because cell viability of all concentration groups is  ≥ 60%.

scholarly journals Biological and physico-mechanical properties of poly(methyl methacrylate) enriched with graphene oxide as a potential biomaterial.

2021 ◽  
Vol 10 (2) ◽  
pp. 1-9
Author(s):  
René García-Contreras ◽  
◽  
Héctor Guzmán-Juárez ◽  
Daniel López-Ramos ◽  
Carlos Alvarez-Gayosso ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Proliferation ◽  
Graphene Oxide ◽  
Methyl Methacrylate ◽  
Cellular Proliferation ◽  
Testing Machine ◽  
Physical And Mechanical Properties ◽  
Biological Properties ◽  
Mechanical Tests ◽  
Poly Methyl Methacrylate

Objective: To determine the cytotoxicity and effects of graphene oxide (GO) on cellular proliferation of gingival-fibroblasts, pulpdental cells and human osteoblasts in culture, and to determine the physical, mechanical and biological properties of poly (methyl methacrylate) (PMMA) enriched with GO. Material and Methods: T he G O w as c haracterized with SEM. Cytotoxicity and cell proliferation were determined by the MTT bioassay. The physical-mechanical tests (flexural strength and elastic modulus) were carried out with a universal testing machine. Sorption and solubility were determined by weighing before and after drying and immersion in water. Porosity was evaluated by visual inspection. Data were analyzed with Student's t-test and Tukey's post-hoc ANOVA. Results: The GO has a heterogeneous morphology and a particle size of 66.67±64.76 μm. GO has a slight to no-cytotoxicity (>50-75% viability) at 1-30 days, and at 24 hours incubation of PMMA with GO significantly stimulates osteoblasts (45±8%, p<0.01). The physical and mechanical properties of PMMA with GO increase considerably without altering sorption, solubility and porosity. Conclusion: GO alone or with PMMA has an acceptable biocompatibility, could contribute to cell proliferation, cell regeneration and improve the physical-mechanical properties of PMMA.

scholarly journals Nanopigmented Acrylic Resin Cured Indistinctively by Water Bath or Microwave Energy for Dentures

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
L. S. Acosta-Torres ◽  
M. C. Arenas ◽  
R. E. Nuñez­-Anita ◽  
F. H. Barceló-Santana ◽  
C. A. Álvarez-Gayosso ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Methyl Methacrylate ◽  
Acrylic Resin ◽  
Microwave Energy ◽  
Water Bath ◽  
Poly Methyl Methacrylate ◽  
Transverse Strength ◽  
Acrylic Resins

The highlight of this study was the synthesis of nanopigmented poly(methyl methacrylate) nanoparticles that were further processed using a water bath and/or microwave energy for dentures. The experimental acrylic resins were physicochemically characterized, and the adherence ofCandida albicansand biocompatibility were assessed. A nanopigmented acrylic resin cured by a water bath or by microwave energy was obtained. The acrylic specimens possess similar properties to commercial acrylic resins, but the transverse strength and porosity were slightly improved. The acrylic resins cured with microwave energy exhibited reducedC. albicansadherence. These results demonstrate an improved noncytotoxic material for the manufacturing of denture bases in dentistry.

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