Cyanobacteria as Nanogold Factories II: Chemical Reactivity and anti-Myocardial Infraction Properties of Customized Gold Nanoparticles Biosynthesized by Cyanothece sp.

Cyanothece sp., a coccoid, unicellular, nitrogen-fixing and hydrogen-producing cyanobacterium, has been used in this study to biosynthesize customized gold nanoparticles under certain chemical conditions. The produced gold nanoparticles had a characteristic absorption band at 525–535 nm. Two types of gold nanoparticle, the purple and blue, were formed according to the chemical environment in which the cyanobacterium was grown. Dynamic light scattering was implemented to estimate the size of the purple and blue nanoparticles, which ranged from 80 ± 30 nm and 129 ± 40 nm in diameter, respectively. The highest scattering of laser light was recorded for the blue gold nanoparticles, which was possibly due to their larger size and higher concentration. The appearance of anodic and cathodic peaks in cyclic voltammetric scans of the blue gold nanoparticles reflected the oxidation into gold oxide, followed by the subsequent reduction into the nano metal state. The two produced forms of gold nanoparticles were used to treat isoproterenol-induced myocardial infarction in experimental rats. Both forms of nanoparticles ameliorated myocardial infarction injury, with a slight difference in their curative activity with the purple being more effective. Mechanisms that might explain the curative effect of these nanoparticles on the myocardial infarction were proposed. The morphological, physiological, and biochemical attributes of the Cyanothece sp. cyanobacterium were fundamental for the successful production of “tailored” nanoparticles, and complemented the chemical conditions for the differential biosynthesis process. The present research represents a novel approach to manipulate cyanobacterial cells towards the production of different-sized gold nanoparticles whose curative impacts vary accordingly. This is the first report on that type of manipulated gold nanoparticles biosynthesis which will hopefully open doors for further investigations and biotechnological applications.

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Electroactive nanoparticles loaded Silk protein/Chitosan macromolecular injectable hydrogel to improve therapeutic efficacy of mesenchymal stem cells in functional recovery after ischemic myocardial infarction

10.21203/rs.3.rs-383466/v1 ◽

2021 ◽

Author(s):

Zheng Wu ◽

Shujuan Cheng ◽

Shaoping Wang ◽

Wenzheng Li ◽

Jinghua Liu

Keyword(s):

Myocardial Infarction ◽

Stem Cells ◽

Gold Nanoparticles ◽

Mesenchymal Stem Cells ◽

Mechanical Test ◽

Silk Protein ◽

Specific Marker ◽

H9c2 Cells ◽

Myocardial Infraction

Abstract Background Currently, cardiac regeneration by stem cell-based tissue engineering is considered an important strategy for overcoming myocardial infarction. Therefore, this study is designed to explore the potential for differentiation of gold nanoparticles loaded injectable Silk protein/Chitosan hydrogel along with mesenchymal stem cells towards a cardiomyogenic phenotype. Methods The incorporated gold nanoparticles into chitosan-silk fibroin hydrogel (Au@Ch-SF) was validated by various analysis including FT-IR, NMR, XRD and SEM analysis. The major properties of Au@Ch-SF hydrogel such as weight loss, mechanical test and drug releasing activities also investigated. Further, the mesenchymal stem cells (MS) were encapsulated into hydrogel by incubating the MS cells with 100 µg/mL of Au@Ch-SF hydrogel in a humidified incubator at 37°C for 3 days in the presence of 5% CO2. In vitro toxicity effect of MS loaded Au@Ch-SF hydrogel was tested against cardiac myoblast H9C2 cells. Further, the tissue regenerative activities in myocardial infraction rats were examined by histology, apoptosis, and Cx43 cardiac-specific marker analysis. Results The gel formation time of Au@Ch-SF was comparatively lower than Ch and Ch-SF hydrogels which demonstrates the stronger intermolecular interactions between Ch and SF. The toxicity study showed that the prepared MS loaded Au@Ch-SF hydrogels did not possess toxicity against cardiac myoblast H9C2 cells. Further, the myocardial infarction rats were treated with MS loaded Au@Ch-SF hydrogel promotes the cardiac muscle fibers regeneration performance which was confirmed by β-MHC and Cx43 cardiac markers. Conclusions We demonstrate for the first time that encapsulation of MS with Au@Ch-SF hydrogels could promotes tissue regenerative activity in myocardial infraction tissues. The findings of this study suggest that MS encapsulated Au@Ch-SF hydrogels might be useful in the treatment of myocardial infarction.

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Gold nanoparticles enhanced chemiluminescence – a novel approach for sensitive determination of aflatoxin-B1

Analytical Methods ◽

10.1039/c3ay40694f ◽

2013 ◽

Vol 5 (18) ◽

pp. 4838 ◽

Cited By ~ 16

Author(s):

K. S. Abhijith ◽

K. V. Ragavan ◽

M. S. Thakur

Keyword(s):

Gold Nanoparticles ◽

Aflatoxin B1 ◽

Enhanced Chemiluminescence ◽

Novel Approach ◽

Sensitive Determination

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Study of Some Physiological and Biochemical Aspects in The Serum of Myocardial Infarction Patients

Kirkuk University Journal-Scientific Studies ◽

10.32894/kujss.2011.43113 ◽

2011 ◽

Vol 6 (2) ◽

pp. 63-72

Author(s):

Ali M. Sameen ◽

Mohammed Qais Al-Ani

Keyword(s):

Myocardial Infarction ◽

Physiological And Biochemical

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PROGNOSTIC IMPORTANCE OF WBC COUNT IN ACUTE MYOCARDIAL INFRACTION

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v5i5.1903 ◽

2021 ◽

Vol 5 (5) ◽

Author(s):

Mansa Ram Saran ◽

Sukh Chain

Keyword(s):

Myocardial Infarction ◽

High Rate ◽

Inclusion Criteria ◽

Lv Dysfunction ◽

Myocardial Infraction ◽

Acute Mi ◽

Prognostic Importance ◽

Artery Disease ◽

Wbc Count ◽

Global Health Problem

Background: Coronary artery disease is a major cause of death and is a global health problem becoming an epidemic in both developed as well as in developing countries. Methods: 100 patients presenting to hospital within 24hrs with Acute MI and qualifying inclusion criteria were enrolled and written consent was taken regarding participation in the study. Results: In a total of 100 patients participated in our study 69 patients were male and 31 patients were females. Out of 100 patients 26 had LV dysfunction, among these 23 patients 17 were having elevated WBC count and 9 had normal WBC count. Among 100 patients 11 had cardiogenic shock, among these 11 patients 8 had elevated WBC count and 3 had normal WBC count. Conclusion: Elevated WBC count within 24 hours was associated with high rate of complications after acute myocardial infarction. Keywords: STEMI, NSTEMI, WBC

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The Effect of Gold Nanoparticles Combined with High-Quality Nursing in Patients with Myocardial Infarction

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2385 ◽

2021 ◽

Vol 11 (1) ◽

pp. 165-170

Author(s):

Shasha Lv ◽

Ling Yang ◽

Ruifei Wu ◽

Xiaoxing Feng

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Gold Nanoparticles ◽

Molecular Analysis ◽

Healthy Subjects ◽

Control Group ◽

Observation Group ◽

High Quality ◽

Nano Gold

This study was established to study the effect of gold nanoparticles combined with high-quality nursing in patients with acute myocardial infarction (AMI). Under the condition of reflux, a nano gold strip was prepared. One hundred patients with AMI who were treated from April to September 2018 at our Hospital were selected for analysis, divided equally between the observation group and the control group. The associated responses of inflammation factors related to myocardial infarction were determined by molecular analysis. The levels of factors in patients with AMI were significantly higher than those in healthy subjects (P < 0.05). These findings suggest that a nano gold strip can be used for diagnosing early myocardial infarction.

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A novel approach to imaging engorged ticks: Micro-CT scanning of Ixodes ricinus fed on blood enriched with gold nanoparticles

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2020.101559 ◽

2021 ◽

Vol 12 (1) ◽

pp. 101559

Author(s):

Jaroslav Ondruš ◽

František Hubatka ◽

Pavel Kulich ◽

Nikola Odehnalová ◽

Vratislav Harabiš ◽

...

Keyword(s):

Gold Nanoparticles ◽

Ixodes Ricinus ◽

Ct Scanning ◽

Micro Ct ◽

Novel Approach

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Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Journal of Nanoparticles ◽

10.1155/2015/829718 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Kantrao Saware ◽

Ravindra Mahadappa Aurade ◽

P. D. Kamala Jayanthi ◽

Venkataraman Abbaraju

Keyword(s):

Gold Nanoparticles ◽

Silver Nanoparticles ◽

Amylase Activity ◽

Temperature Stability ◽

Fold Increase ◽

Free Enzyme ◽

Catalytic Function ◽

Ficus Benghalensis ◽

Novel Approach ◽

Immobilization Of Enzymes

Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1- to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.

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Influence of Secondary Prevention and Risk Factors to Recurrent Miocard Infark on Acute Coronary Syndrome Patients in General Hospital Surakarta Indonesia

International Journal of Online and Biomedical Engineering (iJOE) ◽

10.3991/ijoe.v15i10.10872 ◽

2019 ◽

Vol 15 (10) ◽

pp. 136

Author(s):

Hidayah Karuniawati Karuniawati ◽

Vionella Moutika Putri ◽

Tuqa Haitham

Keyword(s):

Risk Factors ◽

Myocardial Infarction ◽

Secondary Prevention ◽

Statin Therapy ◽

Antiplatelet Therapy ◽

Ace Inhibitor ◽

Chi Square ◽

Recurrent Myocardial Infarction ◽

Myocardial Infraction ◽

Chi Square Test

<p class="0abstract">The patient who had myocardial infraction has a risk of recurrent myocardial infraction. Secondary prevention including antiplatelet, beta-blocker, statin, ACE inhibitor/ARB aims to prevent recurrent myocardial infarction. This study aimed to find the influence of secondary prevention and risk factors on the occurrence of recurrent myocardial infraction. This research was conducted with quantitative and case-control retrospectively. The subjects were 80 respondents consisting of 40 respondents in the case group and 40 respondents in control group patients. Data were analyzed using bivariate analysis of chi-square test followed by multivariate analysis of logistic regression. Rate of recurrent myocardial infarction (MI) with antiplatelet therapy decreased from 64.3% to 34.2%, with beta-blocker therapy decreased from 57.7% to 35.7%, with statin therapy decreased from 65.9% to 30.5%, with ACE inhibitor/ARB therapy decreased from 65.9% to 30.5%, with a combination of fourth therapy reduced from 57.4% to 34.6%. Chi-square test showed that there was a statistically significant relationship between antiplatelet therapy (p = 0.007), statin therapy (p = 0.002), ACE inhibitor / ARB therapy (p = 0.002), family history (p = 0.011) and adherence (p = 0.007) to recurrent MI. While multivariate analysis of logistic regression showed that the variables influencing the incidence of recurrent myocardial infarction were did not use antiplatelet therapy (P = 0,005; OR= 4.006) and statin therapy (P = 0,029; OR= 3.111). The secondary prevention reduced the incident of recurrent myocardial infarction.</p>

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Proposed percutaneous aortic valve prosthesis made of cryogel

Proceedings of the Institution of Mechanical Engineers Part H Journal of Engineering in Medicine ◽

10.1177/0954411919837302 ◽

2019 ◽

Vol 233 (5) ◽

pp. 515-524 ◽

Cited By ~ 2

Author(s):

Hadi Mohammadi ◽

Dylan Goode ◽

Guy Fradet ◽

Kibret Mequanint

Keyword(s):

Aortic Valve ◽

Heart Valves ◽

Kidney Injury ◽

Blood Stream ◽

Valve Prosthesis ◽

Element Analysis ◽

Novel Approach ◽

Natural Geometry ◽

Myocardial Infraction ◽

Aortic Valve Prosthesis

Transcatheter heart valves are promising for high-risk patients. Generally, their leaflets are made of pericardium stented in a Nitinol basket. Despite their relative success, they are associated with significant complications such as valve migration, implantation risks, stroke, coronary obstruction, myocardial infraction, acute kidney injury (which all are due to the release of detached solid calcific pieces in to the blood stream) and expected issues existing with tissue valves such as leaflet calcification. This study is an attempt to fabricate the first ever polymeric percutaneous valves made of cryogel following the geometry and mechanical properties of porcine aortic valve to address some of the above-mentioned shortcomings. A novel, one-piece, tricuspid percutaneous valve, consisting of leaflets made entirely from the hydrogel, polyvinyl alcohol cryogel reinforced by bacterial cellulose natural nanocomposite, attached to a Nitinol basket was developed and demonstrated. Following the natural geometry of the valve, a novel approach was applied based on the revolution about an axis of a hyperboloid shape. The geometry was modified based on avoiding sharp warpage of leaflets and removal of the central opening orifice area of the valve when valve is fully closed using the finite element analysis. The modified geometry was replaced by a cloud of (control) points and was essentially converted to Bezier surfaces for further adjustment. A cavity mold was then designed and fabricated to form the valve. The fabricated valve was sewn into the Nitinol basket which is covered by Dacron cloth. The models presented in this study merit further development and revisions for both aortic and mitral positions.

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