scholarly journals The Response of Cowpea (Vigna unguiculata) Plants to Three Abiotic Stresses Applied with Increasing Intensity: Hypoxia, Salinity, and Water Deficit

Metabolites ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 38
Author(s):  
Jayamini Jayawardhane ◽  
Juran C. Goyali ◽  
Somaieh Zafari ◽  
Abir U. Igamberdiev
Keyword(s):  
Water Deficit ◽  
Vigna Unguiculata ◽  
Abiotic Stresses ◽  
Soluble Sugar ◽  
No Production ◽  
Emission Rates ◽  
Nitrogen Gas ◽  
Whole Plant ◽  
Nacl Concentration ◽  
Sugar Levels

Exposing plants to gradually increasing stress and to abiotic shock represents two different phenomena. The knowledge on plants’ responses following gradually increasing stress is limited, as many of the studies are focused on abiotic shock responses. We aimed to investigate how cowpea (Vigna unguiculata (L.) Walp.) plants respond to three common agricultural abiotic stresses: hypoxia (applied with the increasing time of exposure to nitrogen gas), salinity (gradually increasing NaCl concentration), and water deficit (gradual decrease in water supply). We hypothesized that the cowpea plants would increase in tolerance to these three abiotic stresses when their intensities rose in a stepwise manner. Following two weeks of treatments, leaf and whole-plant fresh weights declined, soluble sugar levels in leaves decreased, and lipid peroxidation of leaves and roots and the levels of leaf electrolyte leakage increased. Polyphenol oxidase activity in both roots and leaves exhibited a marked increase as compared to catalase and peroxidase. Leaf flavonoid content decreased considerably after hypoxia, while it increased under water deficit treatment. NO emission rates after 3 h in the hypoxically treated plants were similar to the controls, while the other two treatments resulted in lower values of NO production, and these levels further decreased with time. The degree of these changes was dependent on the type of treatment, and the observed effects were more substantial in leaves than in roots. In summary, the responses of cowpea plants to abiotic stress depend on the type and the degree of stress applied and the plant organs.

Relationships between biomass allocation, axis organogenesis and organ expansion under shading and water deficit conditions in grapevine

2015 ◽  
Vol 42 (12) ◽  
pp. 1116 ◽  
Author(s):  
Benoît Pallas ◽  
Angélique Christophe
Keyword(s):  
Water Stress ◽  
Plant Growth ◽  
Leaf Area ◽  
Water Deficit ◽  
Biomass Allocation ◽  
Abiotic Stresses ◽  
Sink Strength ◽  
Secondary Axis ◽  
Whole Plant ◽  
Abiotic Constraints

The relationships between whole-plant growth and morphogenetic processes under abiotic stresses are still partly unknown. Whole-plant biomass growth can be decreased by many abiotic stresses, including water deficit and shading. Two experiments were performed on potted plants of one grapevine cultivar (Vitis vinifera L. cv. Syrah) subjected to watering and shading treatments. Under water stress, plants reduced their primary and secondary axis leaf production rate, whereas secondary axis budburst was relatively unaffected. Individual leaf area was reduced and a strong decrease in leaf expansion rate was observed. Under shading, primary axis organogenesis was maintained, both secondary axis budburst rate and phytomer appearance rate were decreased, and individual leaf area slightly increased. Specific leaf area did not change under soil water deficit, whereas it increased under shading. These results confirm the existence of dynamic changes in organ sink strength and biomass allocation patterns to favour plant leaf area growth under shading, and to reduce plant leaf area and water losses by transpiration under water stress. From a modelling point of view, this study shows that functional structural models based on a C balance are not fully relevant for simulating plant growth under abiotic constraints if they do not include non-trophic relationships (hormonal signalling or plant hydraulic properties) that modify organ sink strength according to abiotic constraints.

Manipulating fruit chloroplasts as a strategy to improve fruit quality

2013 ◽  
Author(s):  
Alan B. Bennett ◽  
Arthur A. Schaffer ◽  
Ilan Levin ◽  
Marina Petreikov ◽  
Adi Doron-Faigenboim
Keyword(s):  
Chloroplast Development ◽  
Tomato Fruit ◽  
Soluble Sugar ◽  
Fruit Size ◽  
Soluble Solids ◽  
Nucleotide Polymorphisms ◽  
Ripe Fruit ◽  
Green Fruit ◽  
Functional Alleles ◽  
Sugar Levels

The Original Objectives were modified and two were eliminated to reflect the experimental results: Objective 1 - Identify additional genetic variability in SlGLK2 and IPin wild, traditional and heirloom tomato varieties Objective 2 - Determine carbon balance and horticultural characteristics of isogenic lines expressing functional and non-functional alleles of GLKsand IP Background: The goal of the research was to understand the unique aspects of chloroplasts and photosynthesis in green fruit and the consequences of increasing the chloroplast capacity of green fruit for ripe fruit sugars, yield, flavor and nutrient qualities. By focusing on the regulation of chloroplast formation and development solely in fruit, our integrated knowledge of photosynthetic structures/organs could be broadened and the results of the work could impact the design of manipulations to optimize quality outputs for the agricultural fruit with enhanced sugars, nutrients and flavors. The project was based on the hypothesis that photosynthetic and non-photosynthetic plastid metabolism in green tomato fruit is controlled at a basal level by light for minimal energy requirements but fruit-specific genes regulate further development of robust chloroplasts in this organ. Our BARD project goals were to characterize and quantitate the photosynthesis and chloroplast derived products impacted by expression of a tomato Golden 2- like 2 transcription factor (US activities) in a diverse set of 31 heirloom tomato lines and examine the role of another potential regulator, the product of the Intense Pigment gene (IP activities). Using tomato Golden 2-like 2 and Intense Pigment, which was an undefined locus that leads to enhanced chloroplast development in green fruit, we sought to determine the benefits and costs of extensive chloroplast development in fruit prior to ripening. Major conclusions, solutions, achievements: Single nucleotide polymorphisms in the promoter, coding and intronicSlGLK2 sequences of 20 heirloom tomato lines were identified and three SlGLK2 promoter lineages were identified; two lineages also had striped fruit variants. Lines with striped fruit but no shoulders were not identified. Green fruit chlorophyll and ripe fruit soluble sugar levels were measured in 31 heirloom varieties and fruit size correlates with ripe fruit sugars but dark shoulders does not. A combination of fine mapping, recombinant generation, RNAseq expression and SNP calling all indicated that the proposed localization of a single locus IP on chr 10 was incorrect. Rather, the IP line harbored 11 separate introgressions from the S. chmielewskiparent, scattered throughout the genome. These introgressions harbored ~3% of the wild species genome and no recombinant consistently recovered the IP parental phenotype. The 11 introgressions were dissected into small combinations in segregating recombinant populations. Based on these analyses two QTL for Brix content were identified, accounting for the effect of increased Brix in the IP line. Scientific and agricultural implications: SlGLK2 sequence variation in heirloom tomato varieties has been identified and can be used to breed for differences in SlGLK2 expression and possibly in the green striped fruit phenotype. Two QTL for Brix content have been identified in the S. chmielewskiparental line and these can be used for increasing soluble solids contents in breeding programs. 

scholarly journals Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution

2003 ◽  
Vol 60 (2) ◽  
pp. 239-244 ◽  
Author(s):  
José Carlos da Silva ◽  
José Donizeti Alves ◽  
Amauri Alves de Alvarenga ◽  
Marcelo Murad Magalhães ◽  
Dárlan Einstein do Livramento ◽  
...  
Keyword(s):  
Sucrose Synthase ◽  
Sucrose Solution ◽  
Management Practice ◽  
Physiological State ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugar ◽  
Coffee Plants ◽  
Sugar Levels ◽  
High Level

One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and sucrose synthase. In plants with high level of carbon reserve, no increments in total soluble sugar levels or in enzymatic activity were observed. Regardless of treatments or plants physiological state, no differences in transpiration or stomatal conductance were observed, demonstrating the stomatal control of transpiration. Photosynthesis was stimulated with the use of 0.5 and 1.0 % sucrose only in depauperate plants. Coffee seedling spraying with sucrose is only efficient for depauperate plants, at the concentration of 1.0%.

scholarly journals Overexpression of a Banana Aquaporin Gene MaPIP1;1 Enhances Tolerance to Multiple Abiotic Stresses in Transgenic Banana and Analysis of Its Interacting Transcription Factors

2021 ◽  
Vol 12 ◽  
Author(s):  
Yi Xu ◽  
Juhua Liu ◽  
Caihong Jia ◽  
Wei Hu ◽  
Shun Song ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Promoter Region ◽  
Expression Patterns ◽  
Soluble Sugar ◽  
Ion Leakage ◽  
Aquaporin Gene ◽  
Gene Response ◽  
Transcription Factor Genes ◽  
Transgenic Lines ◽  
Response To Stress

Aquaporins can improve the ability of plants to resist abiotic stresses, but the mechanism is still not completely clear. In this research, overexpression of MaPIP1;1 in banana improved tolerance to multiple stresses. The transgenic plants resulted in lower ion leakage and malondialdehyde content, while the proline, chlorophyll, soluble sugar, and abscisic acid (ABA) contents were higher. In addition, under high salt and recovery conditions, the content of Na+ and K+ is higher, also under recovery conditions, the ratio of K+/Na+ is higher. Finally, under stress conditions, the expression levels of ABA biosynthesis and response genes in the transgenic lines are higher than those of the wild type. In previous studies, we proved that the MaMADS3 could bind to the promoter region of MaPIP1;1, thereby regulating the expression of MaPIP1;1 and affecting the drought tolerance of banana plants. However, the mechanism of MaPIP1;1 gene response to stress under different adversity conditions might be regulated differently. In this study, we proved that some transcription factor genes, including MaERF14, MaDREB1G, MaMYB1R1, MaERF1/39, MabZIP53, and MaMYB22, showed similar expression patterns with MaPIP1;1 under salt or cold stresses, and their encoded proteins could bind to the promoter region of MaPIP1;1. Here we proposed a novel MaPIP1;1-mediated mechanism that enhanced salt and cold tolerance in bananas. The results of this study have enriched the stress-resistant regulatory network of aquaporins genes and are of great significance for the development of molecular breeding strategies for stress-resistant fruit crops.

scholarly journals Screening cowpea [Vigna unguiculata (L.) Walp.] varieties by inducing water deficit and RAPD analyses

2003 ◽  
Vol 3 (3) ◽  
pp. 174-178
Author(s):  
Abaye Badiane Franccedil;ois ◽  
Diouf Diaga ◽  
Sane Djibril ◽  
Diouf Omar ◽  
Goudiaby Venceslas ◽  
...  
Keyword(s):  
Water Deficit ◽  
Vigna Unguiculata

scholarly journals Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions

2020 ◽  
Vol 71 (12) ◽  
pp. 3603-3612 ◽  
Author(s):  
Natanella Illouz-Eliaz ◽  
Idan Nissan ◽  
Ido Nir ◽  
Uria Ramon ◽  
Hagai Shohat ◽  
...  
Keyword(s):  
Water Deficit ◽  
Water Status ◽  
Stomatal Closure ◽  
Normal Growth ◽  
Hydraulic Conductance ◽  
Xylem Vessel ◽  
Leaf Water Content ◽  
Whole Plant ◽  
Drought Conditions ◽  
Better Than

Abstract Low gibberellin (GA) activity in tomato (Solanum lycopersicum) inhibits leaf expansion and reduces stomatal conductance. This leads to lower transpiration and improved water status under transient drought conditions. Tomato has three GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors with overlapping activities and high redundancy. We tested whether mutation in a single GID1 reduces transpiration without affecting growth and productivity. CRISPR-Cas9 gid1 mutants were able to maintain higher leaf water content under water-deficit conditions. Moreover, while gid1a exhibited normal growth, it showed reduced whole-plant transpiration and better recovery from dehydration. Mutation in GID1a inhibited xylem vessel proliferation, which led to lower hydraulic conductance. In stronger GA mutants, we also found reduced xylem vessel expansion. These results suggest that low GA activity affects transpiration by multiple mechanisms: it reduces leaf area, promotes stomatal closure, and reduces xylem proliferation and expansion, and as a result, xylem hydraulic conductance. We further examined if gid1a performs better than the control M82 in the field. Under these conditions, the high redundancy of GID1s was lost and gid1a plants were semi-dwarf, but their productivity was not affected. Although gid1a did not perform better under drought conditions in the field, it exhibited a higher harvest index.

scholarly journals Potato Tuberworm Phthorimaea operculella (Zeller) (Lepidoptera: Gelechioidea) Leaf Infestation Effects Performance of Conspecific Larvae on Harvested Tubers by Inducing Chemical Defenses

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 633
Author(s):  
Dingli Wang ◽  
Qiyun Wang ◽  
Xiao Sun ◽  
Yulin Gao ◽  
Jianqing Ding
Keyword(s):  
Soluble Sugar ◽  
C:N Ratio ◽  
Developmental Time ◽  
Chemical Defenses ◽  
Pupal Weight ◽  
Larval Performance ◽  
Aboveground Herbivory ◽  
Whole Plant ◽  
Potato Tuberworm

Conspecific aboveground and belowground herbivores can interact with each other, mediated by plant secondary chemicals; however, little attention has been paid to the interaction between leaf feeders and tuber-feeders. Here, we evaluated the effect of the foliar feeding of P. operculella larvae on the development of conspecific larvae feeding on harvested tubers by determining the nutrition and defense metabolites in the whole plant (leaf, root and tuber). We found that leaf feeding negatively affected tuber larval performance by increasing the female larval developmental time and reducing the male pupal weight. In addition, aboveground herbivory increased α-chaconine and glycoalkaloids in tubers and α-solanine in leaves, but decreased α-chaconine and glycoalkaloids in leaves. Aboveground herbivory also altered the levels of soluble sugar, soluble protein, starch, carbon (C), nitrogen (N), as well as the C:N ratio in both leaves and tubers. Aboveground P. operculella infestations could affect the performance of conspecific larvae feeding on harvested tubers by inducing glycoalkaloids in the host plant. Our findings indicate that field leaf herbivory should be considered when assessing the quality of potato tubers and their responses to pests during storage.

scholarly journals Physiological tolerance to drought under high temperature in soybean cultivars

2019 ◽  
pp. 976-987 ◽  
Author(s):  
Jônatas Neves de Castro ◽  
Caroline Müller ◽  
Gabriel Martins Almeida ◽  
Alan Carlos Costa
Keyword(s):  
High Temperature ◽  
Water Deficit ◽  
Electron Flow ◽  
Soluble Sugar ◽  
Water Levels ◽  
Effective Quantum Yield ◽  
Growth Responses ◽  
Ps Ii ◽  
Total Electron ◽  
Soybean Cultivars

Soybean is one of the most economically important crops and has experienced adverse physiological and biochemical effects when subjected to drought stress and heat, resulting in lost productivity. Thus, the objective of this work was to evaluate the physiological, metabolic and growth responses of well-watered and drought-treated soybean cultivars under high temperature. The experimental design was set up in randomized blocks, in a factorial scheme with three soybean cultivars (7739 M, Anta 82 and Desafio) and two water levels (100% and 40% field capacity). The experiment was conducted in a controlled growth chamber with a gradual rise in temperature at 41°C for 5 hours daily. Morpho-physiological and metabolic analyses were performed 12 days after the treatments imposition. The parameters of water and osmotic potentials, relative water content, photosynthetic rate, stomatal conductance, transpiratory rate, electron flux for the carboxylation and oxygenation of RuBisCO were decreased for all cultivars under water deficit and high temperature. The results showed that the photorespiration and the rate of electrolyte leakage were increased as well. These results showed that these physiological behaviors are standard for soybean plants under water deficit, regardless of cultivars. The cultivars 7739 M and Desafio showed lower performance than the cultivar Anta 82 for the parameters of total electron flow and effective quantum yield of PS II. The 7739 M and Anta 82 were the only cultivars to show increased non-photochemical quenching dissipation and total soluble sugar content, respectively, under stress conditions. Desafio cultivar demonstrated greater physiological and growth traits stability, which could potentially indicate double tolerance to these stresses.

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