scholarly journals A Potential Application of Triangular Microwells to Entrap Single Cancer Cells: A Canine Cutaneous Mast Cell Tumor Model

Micromachines ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 841
Author(s):  
Dettachai Ketpun ◽  
Alongkorn Pimpin ◽  
Tewan Tongmanee ◽  
Sudchaya Bhanpattanakul ◽  
Prapruddee Piyaviriyakul ◽  
...  
Keyword(s):  
Mast Cell ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Tumor ◽  
Cellular Heterogeneity ◽  
Cell Analysis ◽  
Mast Cell Tumor ◽  
Multiple Cell ◽  
Cutaneous Mast Cell ◽  
The Impact

Cellular heterogeneity is a major hindrance, leading to the misunderstanding of dynamic cell biology. However, single cell analysis (SCA) has been used as a practical means to overcome this drawback. Many contemporary methodologies are available for single cell analysis; among these, microfluidics is the most attractive and effective technology, due to its advantages of low-volume specimen consumption, label-free evaluation, and real-time monitoring, among others. In this paper, a conceptual application for microfluidic single cell analysis for veterinary research is presented. A microfluidic device is fabricated with an elastomer substrate, polydimethylsiloxane (PDMS), under standard soft lithography. The performance of the microdevice is high-throughput, sensitive, and user-friendly. A total of 53.1% of the triangular microwells were able to trap single canine cutaneous mast cell tumor (MCT) cells. Of these, 38.82% were single cell entrapments, while 14.34% were multiple cell entrapments. The ratio of single-to-multiple cell trapping was high, at 2.7:1. In addition, 80.5% of the trapped cells were viable, indicating that the system was non-lethal. OCT4A-immunofluorescence combined with the proposed system can assess OCT4A expression in trapped single cells more precisely than OCT4A-immunohistochemistry. Therefore, the results suggest that microfluidic single cell analysis could potentially reduce the impact of cellular heterogeneity.

scholarly journals RETRACTED ARTICLE: Cutaneous mast cell tumor (Mastocytoma): Cyto- histopathological and haematological investigations

2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Ehsan Hosseini ◽  
Behnam Pedram ◽  
Ali Mohammad Bahrami ◽  
Mohammad Hossein Jaberi Moghaddam ◽  
Javad Javanbakht ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Tumor ◽  
Mast Cell Tumor ◽  
Retracted Article ◽  
Cutaneous Mast Cell

scholarly journals Single-cell analysis reveals cellular heterogeneity and molecular determinants of hypothalamic leptin-receptor cells

2020 ◽  
Author(s):  
N. Kakava-Georgiadou ◽  
J.F. Severens ◽  
A.M. Jørgensen ◽  
K.M. Garner ◽  
M.C.M Luijendijk ◽  
...  
Keyword(s):  
Single Cell ◽  
Leptin Receptor ◽  
Single Cell Analysis ◽  
Cell Types ◽  
Cellular Heterogeneity ◽  
Molecular Signature ◽  
Neuronal Populations ◽  
Hypothalamic Nuclei ◽  
Satiety Hormone ◽  
Multiple Cell

AbstractHypothalamic nuclei which regulate homeostatic functions express leptin receptor (LepR), the primary target of the satiety hormone leptin. Single-cell RNA sequencing (scRNA-seq) has facilitated the discovery of a variety of hypothalamic cell types. However, low abundance of LepR transcripts prevented further characterization of LepR cells. Therefore, we perform scRNA-seq on isolated LepR cells and identify eight neuronal clusters, including three uncharacterized Trh-expressing populations as well as 17 non-neuronal populations including tanycytes, oligodendrocytes and endothelial cells. Food restriction had a major impact on Agrp neurons and changed the expression of obesity-associated genes. Multiple cell clusters were enriched for GWAS signals of obesity. We further explored changes in the gene regulatory landscape of LepR cell types. We thus reveal the molecular signature of distinct populations with diverse neurochemical profiles, which will aid efforts to illuminate the multi-functional nature of leptin’s action in the hypothalamus.

scholarly journals Retraction note: Cutaneous mast cell tumor (Mastocytoma): cyto-histopathological and haematological investigations

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Ehsan Hosseini ◽  
Behnam Pedram ◽  
Ali Mohammad Bahrami ◽  
Mohammad Hossein Jaberi Moghaddam ◽  
Javad Javanbakht ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Tumor ◽  
Mast Cell Tumor ◽  
Cutaneous Mast Cell

18FDG-PET IMAGING IN CANINE LYMPHOMA AND CUTANEOUS MAST CELL TUMOR

2009 ◽  
Vol 50 (2) ◽  
pp. 215-223 ◽  
Author(s):  
AMY K. LEBLANC ◽  
BJOERN W. JAKOBY ◽  
DAVID W. TOWNSEND ◽  
GREGORY B. DANIEL
Keyword(s):  
Mast Cell ◽  
Pet Imaging ◽  
Cell Tumor ◽  
Canine Lymphoma ◽  
18Fdg Pet ◽  
Mast Cell Tumor ◽  
Cutaneous Mast Cell

scholarly journals The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model

Micromachines ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 9 ◽  
Author(s):  
Dettachai Ketpun ◽  
Achariya Sailasuta ◽  
Thammawit Suwannaphan ◽  
Sudchaya Bhanpattanakul ◽  
Alongkorn Pimpin ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cancer Cells ◽  
Tumor Model ◽  
Cell Tumor ◽  
Shear Stresses ◽  
Mast Cell Tumor ◽  
Hydrodynamic Shear ◽  
Single Cancer ◽  
Cutaneous Mast Cell ◽  
Spiral Microchannel

scholarly journals Single-Cell Analysis of Antigen-Specific CD8+ T-Cell Transcripts Reveals Profiles Specific to mRNA or Adjuvanted Protein Vaccines

2021 ◽  
Vol 12 ◽  
Author(s):  
Trine Sundebo Meldgaard ◽  
Fabiola Blengio ◽  
Denise Maffione ◽  
Chiara Sammicheli ◽  
Simona Tavarini ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Single Cell ◽  
Cd8 T Cells ◽  
Single Cell Analysis ◽  
Expression Profiles ◽  
Cd8 T Cell ◽  
Cellular Heterogeneity ◽  
Cell Analysis

CD8+ T cells play a key role in mediating protective immunity after immune challenges such as infection or vaccination. Several subsets of differentiated CD8+ T cells have been identified, however, a deeper understanding of the molecular mechanism that underlies T-cell differentiation is lacking. Conventional approaches to the study of immune responses are typically limited to the analysis of bulk groups of cells that mask the cells’ heterogeneity (RNA-seq, microarray) and to the assessment of a relatively limited number of biomarkers that can be evaluated simultaneously at the population level (flow and mass cytometry). Single-cell analysis, on the other hand, represents a possible alternative that enables a deeper characterization of the underlying cellular heterogeneity. In this study, a murine model was used to characterize immunodominant hemagglutinin (HA533-541)-specific CD8+ T-cell responses to nucleic- and protein-based influenza vaccine candidates, using single-cell sorting followed by transcriptomic analysis. Investigation of single-cell gene expression profiles enabled the discovery of unique subsets of CD8+ T cells that co-expressed cytotoxic genes after vaccination. Moreover, this method enabled the characterization of antigen specific CD8+ T cells that were previously undetected. Single-cell transcriptome profiling has the potential to allow for qualitative discrimination of cells, which could lead to novel insights on biological pathways involved in cellular responses. This approach could be further validated and allow for more informed decision making in preclinical and clinical settings.

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