scholarly journals High-Sensitivity Enzymatic Glucose Sensor Based on ZnO Urchin-like Nanostructure Modified with Fe3O4 Magnetic Particles

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 977
Author(s):  
Qi Mao ◽  
Weixuan Jing ◽  
Weizhuo Gao ◽  
Zhengying Wei ◽  
Bian Tian ◽  
...  
Keyword(s):  
Magnetic Particles ◽  
Glucose Sensor ◽  
Strong Support ◽  
Catalytic Efficiency ◽  
High Sensitivity ◽  
Glucose Sensors ◽  
Enzyme Adsorption ◽  
Test Environment ◽  
Detection Range ◽  
Ultrahigh Sensitivity

A novel and efficient enzymatic glucose sensor was fabricated based on Fe3O4 magnetic nanoparticles (Fe3O4MNPs)-modified urchin-like ZnO nanoflowers (ZnONFs). ZnONFs were hydrothermally synthesizing on a flexible PET substrate. Fe3O4MNPs were deposited on the surface of the ZnONFs by the drop-coating process. The results showed that the urchin-like ZnONFs provided strong support for enzyme adsorption. For Fe3O4MNPs, it significantly promoted the redox electron transfer from the active center of GOx to the ZnO nanoflowers beneath. More importantly, it promoted the hydrolysis of H2O2, the intermediate product of glucose catalytic reaction, and thus improved the electron yield. The sensitivity of the Nafion/GOx/Fe3O4MNPs/ZnONFs/Au/PET sensor was up to 4.52 μA·mM−1·cm−2, which was improved by 7.93 times more than the Nafion/GOx/ZnONFs/Au/PET sensors (0.57 μA·mM−1·cm−2). The detection limit and linear range were also improved. Additionally, the as-fabricated glucose sensors show strong anti-interference performance in the test environment containing organic compounds (such as urea, uric acid, and ascorbic acid) and inorganic salt (for instance, NaCl and KCl). The glucose sensor’s service life was evaluated, and it can still maintain about 80% detection performance when it was reused about 20 times. Compared with other existing sensors, the as-fabricated glucose sensor exhibits an ultrahigh sensitivity and wide detection range. In addition, the introduction of Fe3O4MNPs optimized the catalytic efficiency from the perspective of the reaction mechanism and provided potential ideas for improving the performance of other enzymatic biosensors.

An Electrochemically Reduced Copper/Reduced Graphene Oxide Film Modified Electrode for Sensitive Non-Enzymatic Glucose Detection in Human Serum

2021 ◽  
Author(s):  
Sopit Phetsang ◽  
Pinit Kidkhunthod ◽  
Narong Chanlek ◽  
Jaroon Jakmunee ◽  
Pitchaya Mungkornasawakul ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Human Serum ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Detection Range ◽  
Reduced Graphene

Abstract Numerous studies suggest that modification with functional nanomaterials can enhance the electrode electrocatalytic activity, sensitivity, and selectivity of the electrochemical sensors. Here, a highly sensitive and cost-effective disposable non-enzymatic glucose sensor based on copper(II)/reduced graphene oxide modified screen-printed carbon electrode is demonstrated. Facile fabrication of the developed sensing electrodes is carried out by the adsorption of copper(II) onto graphene oxide modified electrode, then following the electrochemical reduction. The proposed sensor illustrates good electrocatalytic activity toward glucose oxidation with a wide linear detection range from 0.10 mM to 12.5 mM, low detection limit of 65 µM, and high sensitivity of 172 µA mM− 1 cm− 2 along with satisfactory anti-interference ability, reproducibility, stability, and the acceptable recoveries for the detection of glucose in a human serum sample (95.6–106.4%). The copper(II)/reduced graphene oxide based sensor with the superior performances is a great potential for the quantitation of glucose in real samples.

Cuprous Oxide Films with Hollow Cubic Cage Structure for Nonenzymatic Glucose Detection

NANO ◽  
2019 ◽  
Vol 14 (04) ◽  
pp. 1950045
Author(s):  
Fang Sun ◽  
Lehong Xing ◽  
Xihui Yang ◽  
Hailiang Huang ◽  
Lina Ning
Keyword(s):  
Electrode Materials ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
Fast Response ◽  
Glucose Sensing ◽  
Glucose Sensors ◽  
Cage Structure ◽  
Active Materials ◽  
Nonenzymatic Glucose Sensor ◽  
Step Procedure

In this study, CuO films with hollow cubic cages were prepared by a facile two-step procedure consisting of electrodeposition synthesis and subsequent direct calcination. First, Cu2O nanocubes were fabricated on ITO substrate through a simple electrodeposition procedure. Then, Cu2O nanocubes were converted to CuO hollow cubic cages without obvious morphological change through direct calcination. The obtained CuO cubic cages serving as active materials illustrated a favorable performance for nonenzymatic glucose sensing with high sensitivity of [Formula: see text]A[Formula: see text]mM[Formula: see text][Formula: see text]cm[Formula: see text] at a low applied potential of 0.50[Formula: see text]V, fast-response time (less than 3[Formula: see text]s), low detection limit of 1.0[Formula: see text][Formula: see text]M and wide linear range up from 2.0[Formula: see text][Formula: see text]M to 1.0[Formula: see text]mM ([Formula: see text]). Moreover, the good selectivity of the CuO cubic cages-based nonenzymatic glucose sensor against electroactive compounds such as ascorbic acid, uric acid and dopamine were also demonstrated. These good features indicate that the as-prepared CuO cubic cages can be used as promising electrode materials, which have a great potential in the development of sensitive and selective nonenzymatic glucose sensors.

scholarly journals Nanostructured copper selenide as an ultrasensitive and selective non-enzymatic glucose sensor

2021 ◽  
Author(s):  
Siddesh Umapathi ◽  
Harish Singh ◽  
Jahangir Masud ◽  
Manashi Nath
Keyword(s):  
Detection Limit ◽  
High Efficiency ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
Copper Selenide ◽  
Glucose Detection ◽  
Applied Potential ◽  
Detection Range ◽  
Low Detection Limit ◽  
Linear Detection

CuSe nanostructures exhibit high-efficiency for glucose detection with high sensitivity (19.419 mA mM−1 cm−2) and selectivity at low applied potential (0.15 V vs. Ag|AgCl), low detection limit (0.196 μM) and linear detection range (100 nM to 40 μM).

Graphene Nanostructures as Nonenzymatic Glucose Sensor

2021 ◽  
pp. 157-196
Keyword(s):  
Quantitative Analysis ◽  
Detection Limit ◽  
Glucose Sensor ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Glucose Sensors ◽  
Nonenzymatic Glucose Sensor ◽  
Industrial Sectors ◽  
Electrochemically Active ◽  
Increasing Demand

The increasing demand for the development of highly selective and sensitive nonenzymatic electrochemical sensors for the qualitative and quantitative analysis of glucose in pharmaceutical, clinical and industrial sectors has gained enormous attention towards the use of graphene and its derivatives. This chapter describes the efficient development of electrochemically active nonenzymatic glucose sensors using graphene and its composites, achieving high sensitivity, stability, low detection limit, wide linear range and reproducibility.

scholarly journals Direct Growth of Copper Oxide Films on Ti Substrate for Nonenzymatic Glucose Sensors

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Xiaoxu Ji ◽  
Aihua Wang ◽  
Qinghuai Zhao
Keyword(s):  
Copper Oxide ◽  
Glucose Sensor ◽  
Low Cost ◽  
High Sensitivity ◽  
Fast Response ◽  
Glucose Sensors ◽  
Electrochemical Performances ◽  
Nonenzymatic Glucose Sensor ◽  
Long Term Stability ◽  
Direct Growth

Copper oxide (CuO) films directly grown on Ti substrate have been successfully prepared via a hydrothermal method and used to construct an amperometric nonenzymatic glucose sensor. XRD and SEM were used to characterize the samples. The electrochemical performances of the electrode for detection of glucose were investigated by cyclic voltammetry and chronoamperometry. The CuO films based glucose sensors exhibit enhanced electrocatalytic properties which show very high sensitivity (726.9 μA mM−1 cm−2), low detection limit (2 μM), and fast response (2 s). In addition, reproducibility and long-term stability have been observed. Low cost, convenience, and biocompatibility make the CuO films directly grown on Ti substrate electrodes a promising platform for amperometric nonenzymatic glucose sensor.

scholarly journals Copper/reduced graphene oxide film modified electrode for non-enzymatic glucose sensing application

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sopit Phetsang ◽  
Pinit Kidkhunthod ◽  
Narong Chanlek ◽  
Jaroon Jakmunee ◽  
Pitchaya Mungkornasawakul ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Detection Range ◽  
Reduced Graphene

AbstractNumerous studies suggest that modification with functional nanomaterials can enhance the electrode electrocatalytic activity, sensitivity, and selectivity of the electrochemical sensors. Here, a highly sensitive and cost-effective disposable non-enzymatic glucose sensor based on copper(II)/reduced graphene oxide modified screen-printed carbon electrode is demonstrated. Facile fabrication of the developed sensing electrodes is carried out by the adsorption of copper(II) onto graphene oxide modified electrode, then following the electrochemical reduction. The proposed sensor illustrates good electrocatalytic activity toward glucose oxidation with a wide linear detection range from 0.10 mM to 12.5 mM, low detection limit of 65 µM, and high sensitivity of 172 μA mM–1 cm–2 along with satisfactory anti-interference ability, reproducibility, stability, and the acceptable recoveries for the detection of glucose in a human serum sample (95.6–106.4%). The copper(II)/reduced graphene oxide based sensor with the superior performances is a great potential for the quantitation of glucose in real samples.

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

1990 ◽  
Vol 48 (3) ◽  
pp. 860-861
Author(s):  
Lorna K. Mayo ◽  
Kenneth C. Moore ◽  
Mark A. Arnold
Keyword(s):  
Glucose Sensor ◽  
Low Voltage ◽  
Glucose Sensors ◽  
Polymeric Membranes ◽  
Artificial Endocrine Pancreas ◽  
Self Monitoring ◽  
Conducting Materials ◽  
Insulin Dependent ◽  
Living Tissues ◽  
Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

scholarly journals Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 208
Author(s):  
Hong Dinh Duong ◽  
Jong Il Rhee
Keyword(s):  
Limit Of Detection ◽  
Oxygen Sensing ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Oxidation Reactions ◽  
Lactate Oxidase ◽  
Lactate Oxidation ◽  
Detection Range ◽  
Quenching Effect ◽  
Sensing Membrane

In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1–2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1–0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

scholarly journals An Ultrahigh-Sensitivity Graphene Resonant Gyroscope

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1890
Author(s):  
Yang Lu ◽  
Zhan-She Guo ◽  
Shang-Chun Fan
Keyword(s):  
Angular Velocity ◽  
High Sensitivity ◽  
Velocity Variation ◽  
Beam Length ◽  
Sensing Element ◽  
Finite Element Software ◽  
Resonant Beam ◽  
Frequency Output ◽  
Length Width ◽  
Ultrahigh Sensitivity

In this study, a graphene beam was selected as a sensing element and used to form a graphene resonant gyroscope structure with direct frequency output and ultrahigh sensitivity. The structure of the graphene resonator gyroscope was simulated using the ANSYS finite element software, and the influence of the length, width, and thickness of the graphene resonant beam on the angular velocity sensitivity was studied. The simulation results show that the resonant frequency of the graphene resonant beam decreased with increasing the beam length and thickness, while the width had a negligible effect. The fundamental frequency of the designed graphene resonator gyroscope was more than 20 MHz, and the sensitivity of the angular velocity was able to reach 22,990 Hz/°/h. This work is of great significance for applications in environments that require high sensitivity to extremely weak angular velocity variation.

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

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