scholarly journals Investigation of Growth Factor and Tenocyte Proliferation Induced by Platelet Rich Plasma (PRP) in a 3-Chamber Co-Culture Device

Micromachines ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 446
Author(s):  
Chih-Hao Chiu ◽  
Rei Higashikawa ◽  
Wen-Ling Yeh ◽  
Kin Lei ◽  
Alvin Chen
Keyword(s):  
Platelet Rich Plasma ◽  
Lateral Epicondylitis ◽  
Rotator Cuff Tears ◽  
Preparation Procedure ◽  
Soft Tissue Healing ◽  
Tissue Healing ◽  
Platelet Number ◽  
Platelet Concentration ◽  
Practical Tool ◽  
Basic Studies

The platelet-rich plasma (PRP) has become an attractive topic for soft tissue healing therapy recently. While some clinical reports revealed the effective treatments for knee osteoarthritis, lateral epicondylitis, and rotator cuff tears, other case studies showed that there was no statistically significant healing improvement. The efficacy of the PRP therapy is still unclear clinically. Thus, a significant amount of basic studies should be conducted to optimize the preparation procedure and the platelet concentration of the PRP. In this work, a 3-chamber co-culture device was developed for the PRP study in order to reduce the usage of primary cells and to avoid the PRP gelation effect. The device was a culture, well partitioning into 3 sub-chambers. Tenocytes and PRP could be respectively loaded into the sub-chambers and co-cultured under the interlinked medium. The results showed that a higher platelet number in the PRP could diffuse higher concentration of the growth factors in the medium and induce higher tenocyte proliferation. The 3-chamber co-culture device provides a simple and practical tool for the PRP study. It is potentially applied for optimizing the preparation procedure and platelet concentration of the PRP therapy.

scholarly journals Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note

2015 ◽  
Vol 42 (6) ◽  
pp. 421-423 ◽  
Author(s):  
Carlos Fernando de Almeida Barros Mourão ◽  
Helder Valiense ◽  
Elias Rodrigues Melo ◽  
Natália Belmock Mascarenhas Freitas Mourão ◽  
Mônica Diuana-Calasans Maia
Keyword(s):  
Bone Graft ◽  
Platelet Rich Plasma ◽  
Technical Note ◽  
Platelet Concentrates ◽  
Soft Tissue Healing ◽  
Liquid Form ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Autologous Platelet ◽  
Injectable Form

The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form ofplatelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form.

scholarly journals Effect of Platelet-Rich Plasma and Amniotic Membrane in Patients with Rotator Cuff Repair

2018 ◽  
Vol 39 ◽  
pp. 98-102
Author(s):  
Heri Suroto ◽  
Ameria Pribadi ◽  
Dwikora Novembri Utomo ◽  
Ferdiansyah Mahyudin ◽  
Lukas Widhiyanto
Keyword(s):  
Soft Tissue ◽  
Rotator Cuff ◽  
Pain Score ◽  
Amniotic Membrane ◽  
Rotator Cuff Repair ◽  
Platelet Rich Plasma ◽  
Soft Tissue Healing ◽  
Disability Score ◽  
Tissue Healing ◽  
Cuff Repair

Rotator cuff disorders are the most common source of shoulder problems, ranging from mild strain to massive tears. Platelet-rich plasma (PRP), an autologous blood with platelets concentration above baseline values represents a source of multiple growth factors that promotes tissue repair. This review examines the potential of using PRP to augment rotator cuff repair. Reporting 4 patients with impingement syndrome and supraspinatus tear who underwent decompression acromioplasty and supraspinatus repair augmented with platelet-rich plasma and amniotic membrane. An evaluation was made 3-24 months postoperative using Shoulder Pain and Disability Index (SPADI). Average preoperative pain score is 64%, disability score 54.58%, and total score 58.19%. Average postoperative pain score is 0%, disability score 0.42%, and total score 0.26% (Minimum Detectable Change at 90% confidence for pain score is 18%, disability score 13%, and total score 11%). This result is consequent with research by Luoay Fallouh, stating that improvement is caused by growth factor effects in platelet-rich plasma which promotes soft tissue healing. It can be concluded that platelet-rich plasma and amniotic membrane have promising effects to enhance soft tissue healing in patients with rotator cuff syndrome. Shoulder function is restored with no limitation on daily activity and pain is no longer present.

scholarly journals Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

2019 ◽  
Vol 63 (2) ◽  
pp. 235-242
Author(s):  
Joanna Wessely-Szponder ◽  
Tomasz Szponder ◽  
Ryszard Bobowiec ◽  
Joanna Michalska
Keyword(s):  
Antimicrobial Activity ◽  
Growth Factors ◽  
Soft Tissue ◽  
Platelet Rich Plasma ◽  
Innate Immune ◽  
Ros Generation ◽  
Soft Tissue Healing ◽  
Tissue Healing ◽  
Functional Features ◽  
Stimulation Of

AbstractIntroduction: Antimicrobial peptides (AMP) are a large group of innate immune effectors, which apart from antimicrobial activity show immunomodulative properties. Platelet-rich plasma (PRP) is a source of autologous growth factors and is used for stimulation of bone and soft tissue healing. The purpose of this study was to assess the influence of PRP and AMP extract on ovine monocyte-derived macrophage cultures. Material and Methods: The study was conducted on ovine macrophages (Mfs) previously stimulated with LPS or dexamethasone and then with preparations of PRP or AMP. Following activation of the Mfs their morphological and functional features were assessed. Results: The study revealed pro-inflammatory influence of both examined preparations on Mfs cultures on the basis of morphology, ROS generation and arginase activity. Both preparations enhanced the pro-inflammatory response of cultured Mfs. Conclusion: This activity may intensify the antimicrobial action of Mfs, however, in cases of excessive and prolonged inflammation the use of these preparations should be limited.

scholarly journals Platelet-rich plasma ameliorates senescence-like phenotypes in a cellular photoaging model

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3152-3160 ◽  
Author(s):  
Chuanlong Jia ◽  
Yongzhou Lu ◽  
Bo Bi ◽  
Liang Chen ◽  
Qingjian Yang ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Blood Plasma ◽  
Platelet Rich Plasma ◽  
Soft Tissue Healing ◽  
Tissue Healing

Platelet-rich plasma (PRP) is a portion of blood plasma enriched with platelets widely investigated for accelerating bone and soft tissue healing.

scholarly journals Comparative evaluation of platelet rich plasma in socket healing and bone regeneration after surgical removal of impacted mandibular third molars

2018 ◽  
Vol 12 (3) ◽  
pp. 153-158 ◽  
Author(s):  
Ravi Bhujbal ◽  
Neelima A Malik ◽  
Nilesh Kumar ◽  
Suresh KV ◽  
Mushtaq I Parkar ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Density ◽  
Platelet Rich Plasma ◽  
Postoperative Recovery ◽  
Control Site ◽  
Surgical Removal ◽  
Third Molars ◽  
Soft Tissue Healing ◽  
Tissue Healing ◽  
Mandibular Third Molars

Background. Surgical removal of mandibular third molars results in pain, swelling and bony defects, causing prolonged postoperative recovery. The growth factors present in platelet-rich plasma (PRP) can accelerate the healing, thereby shortening postoperative recovery period. This study was undertaken to evaluate the role of PRP in postoperative socket healing, pain, swelling and bone regeneration following surgical removal of impacted mandibular third molars. Methods. The present case‒control study was conducted on 20 patients with identical bilateral mandibular third molar impaction. PRP was placed randomly on one side of 3rd molar extraction socket and the contralateral side was used as control. Evaluation of soft tissue healing, pain, swelling and radiologic bone density was carried out. Results. Soft tissue healing was better in the PRP compared to the control site. Immediate postoperative assessment of pain scores showed no significant difference between the two groups (Mann-Whitney U test). On the 7th day, pain scores were lower in case site compared to the control site. Measurement of swelling on the 1st, 3rd and 7th day showed statistically significant differences between the case and control sites (P<0.0001). Postoperative mean bone density at the 3rd and 6th postoperative months was significantly higher in the case site compared to the control site (P=0.00001). Conclusion. The results showed an improvement in wound healing and swelling and an increase in the bone density at PRP site. The growth factors in PRP would improve the hard and soft tissue healing 3 months after molar surgery.

scholarly journals Relevant Aspects of Centrifugation Step in the Preparation of Platelet-Rich Plasma

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda G. M. Perez ◽  
José Fábio S. D. Lana ◽  
Ana Amélia Rodrigues ◽  
Angela Cristina M. Luzo ◽  
William D. Belangero ◽  
...  
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Centrifugal Acceleration ◽  
Platelet Recovery ◽  
Acceleration Time ◽  
Biological Responses ◽  
Tissue Healing ◽  
Healthy Donors ◽  
Platelet Concentration ◽  
Centrifugation Step

Introduction. Platelet-Rich Plasma (PRP) is rich in growth factors, playing important role in tissue healing. The wide variation of reported protocols for preparation of PRP leads to variable compositions, which induce different biological responses and prevent results comparison. This study aims to highlight relevant aspects of the centrifugation step to obtain reproducible results and overall quality. Material and Methods. Samples of blood were collected from 20 healthy donors that have signed free informed consent. Two centrifugation steps (spins) were analyzed for the influence of centrifugal acceleration, time, processed volume, and platelet gradient. The Pure Platelet-Rich Plasma (P-PRP) was characterized as platelet concentration, integrity, and viability (sP-selectin measurement). Results. Lower centrifugal accelerations favour platelet separation. The processing of 3.5 mL of blood at 100 ×g for 10 min (1st spin), 400 ×g for 10 min (2nd spin), withdrawing 2/3 of remnant plasma, promoted high platelet recovery (70–80%) and concentration (5x) maintaining platelet integrity and viability. The recovery of platelets was reduced for a larger WB volume (8.5 mL) processed. Conclusion. Centrifugal acceleration, time, WB processed volume, and minimization of the platelet gradient before sampling are relevant aspects to ensure reproducible compositions within the autologous nature of PRP.

scholarly journals Platelet rich plasma in treatment of plantar fascitis

2017 ◽  
Vol 3 (3) ◽  
pp. 350
Author(s):  
N. Venkatseh Kumar ◽  
Arvind Kumar S. M. ◽  
Udayamoorthy S. ◽  
Krishna Kumar
Keyword(s):  
Pain Relief ◽  
Plantar Fasciitis ◽  
Platelet Rich Plasma ◽  
Visual Analogue Score ◽  
Soft Tissue Healing ◽  
Pain Scores ◽  
Tissue Healing ◽  
Maximum Pain ◽  
The Mean

<p class="abstract"><strong>Background:</strong> Platelet rich plasma (PRP) has been a breakthrough in the field of medicine especially in the field of orthopaedics for enhancing bone and soft tissue healing. Plantar Fasciitis is a very common problem in the field of orthopaedics and are very difficult to treat and a challenge to orthopaedicians. <span lang="EN-IN">The aim of our study was to find out the efficacy of autologous PRP in relieving pain in patients with plantar fasciitis.</span></p><p class="abstract"><strong>Methods:</strong> <span lang="EN-IN">In this study, 25 patients with plantar fasciitis (age above 18 years) were selected. The pain intensity was assessed with visual analogue score initially and during follow up. All subjects were given single autologous intra-lesional PRP injection and the results were assessed using difference in VAS</span>.<strong></strong></p><p class="abstract"><strong>Results:</strong> <span lang="EN-IN">The mean pain score at presentation was 8.08. The mean pain scores at subsequent intervals of 1,2,4,6 months after injection were 4.32, 2.60, 1.88 &amp; 2.00 respectively. (P&lt;0.05 which is statistically significant). The maximum pain relief was seen at 4<sup>th</sup> month post PRP injection and the pain relief was sustained till the last follow up. </span></p><p class="abstract"><strong>Conclusions:</strong> <span lang="EN-IN">From our study we have found that Intra-lesional autologous PRP is effective in relieving pain in patients with plantar fasciitis. PRP is biological and can be a safe adjuvant in the treatment of Plantar Fasciitis.</span></p>

Anti-Inflammatory Effects of Novel Standardized Platelet Rich Plasma Releasates on Knee Osteoarthritic Chondrocytes and Cartilage in vitro

2019 ◽  
Vol 20 (11) ◽  
pp. 920-933 ◽  
Author(s):  
Lucía Gato-Calvo ◽  
Tamara Hermida-Gómez ◽  
Cristina R. Romero ◽  
Elena F. Burguera ◽  
Francisco J. Blanco
Keyword(s):  
Gene Expression ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
Cartilage Explants ◽  
Ex Vivo Model ◽  
Chondrocyte Viability ◽  
Platelet Concentration ◽  
The Absolute ◽  
Anti Inflammatory

Background: Platelet Rich Plasma (PRP) has recently emerged as a potential treatment for osteoarthritis (OA), but composition heterogeneity hampers comparison among studies, with the result that definite conclusions on its efficacy have not been reached. Objective: 1) To develop a novel methodology to prepare a series of standardized PRP releasates (PRP-Rs) with known absolute platelet concentrations, and 2) To evaluate the influence of this standardization parameter on the anti-inflammatory properties of these PRP-Rs in an in vitro and an ex vivo model of OA. Methods: A series of PRPs was prepared using the absolute platelet concentration as the standardization parameter. Doses of platelets ranged from 0% (platelet poor plasma, PPP) to 1.5·105 platelets/µl. PRPs were then activated with CaCl2 to obtain releasates (PRP-R). Chondrocytes were stimulated with 10% of each PRP-R in serum-free culture medium for 72 h to assess proliferation and viability. Cells were co-stimulated with interleukin (IL)-1β (5 ng/ml) and 10% of each PRP-R for 48 h to determine the effects on gene expression, secretion and intra-cellular content of common markers associated with inflammation, catabolism and oxidative stress in OA. OA cartilage explants were co-stimulated with IL-1β (5 ng/ml) and 10% of either PRP-R with 0.75·105 platelets/µl or PRP-R with 1.5·105 platelets/µl for 21 days to assess matrix inflammatory degradation. Results: Chondrocyte viability was not affected, and proliferation was dose-dependently increased. The gene expression of all pro-inflammatory mediators was significantly and dose-independently reduced, except for that of IL-1β and IL-8. Immunoblotting corroborated this effect for inducible NO synthase (NOS2). Secreted matrix metalloproteinase-13 (MMP-13) was reduced to almost basal levels by the PRP-R from PPP. Increasing platelet dosage led to progressive loss to this anti-catabolic ability. Safranin O and toluidine blue stains supported the beneficial effect of low platelet dosage on cartilage matrix preservation. Conclusion: We have developed a methodology to prepare PRP releasates using the absolute platelet concentration as the standardization parameter. Using this approach, the composition of the resulting PRP derived product is independent of the donor initial basal platelet count, thereby allowing the evaluation of its effects objectively and reproducibly. In our OA models, PRP-Rs showed antiinflammatory, anti-oxidant and anti-catabolic properties. Platelet enrichment could favor chondrocyte proliferation but is not necessary for the above effects and could even be counter-productive.

scholarly journals Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1478
Author(s):  
Lorenzo G. T. M. Segabinazzi ◽  
Giorgia Podico ◽  
Michael F. Rosser ◽  
Som G. Nanjappa ◽  
Marco A. Alvarenga ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Platelet Rich Plasma ◽  
Upright Position ◽  
Platelet Counts ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Platelet Concentration ◽  
Manual Methods ◽  
White Blood Cell Counts ◽  
Over Time

In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

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