scholarly journals Phenazine-1-carboxylic Acid Produced by Pseudomonas chlororaphis YL-1 Is Effective against Acidovorax citrulli

2021 ◽  
Vol 9 (10) ◽  
pp. 2012
Author(s):  
Youzhou Liu ◽  
Yaqiu Zhou ◽  
Junqing Qiao ◽  
Wenjie Yu ◽  
Xiayan Pan ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Bacterial Strain ◽  
Bacterial Pathogen ◽  
Gene Clusters ◽  
Transcript Level ◽  
Pseudomonas Chlororaphis ◽  
Inhibitory Effects ◽  
Acidovorax Citrulli ◽  
Gene Expression Analyses ◽  
Antimicrobial Metabolites

The bacterial pathogen Acidovorax citrulli causes the destructive fruit blotch (BFB) on cucurbit plants. Pseudomonas chlororaphis YL-1 is a bacterial strain isolated from Mississippi soil and its genome harbors some antimicrobial-related gene clusters, such as phenazine, pyrrolnitrin, and pyoverdine. Here, we evaluated the antimicrobial activity of strain YL-1 as compared with its deficient mutants of antimicrobial-related genes, which were obtained using a sacB-based site-specific mutagenesis strategy. We found that only phenazine-deficient mutants ΔphzE and ΔphzF almost lost the inhibitory effects against A. citrulli in LB plates compared with the wild-type strain YL-1, and that the main antibacterial compound produced by strain YL-1 in LB medium was phenazine-1-carboxylic acid (PCA) based on the liquid chromatography-mass spectrometry (LC-MS) analysis. Gene expression analyses revealed that PCA enhanced the accumulation of reactive oxygen species (ROS) and increased the activity of catalase (CAT) in A. citrulli. The inhibition effect of PCA against A. citrulli was lowered by adding exogenous CAT. PCA significantly upregulated the transcript level of katB from 6 to 10 h, which encodes CAT that helps to protect the bacteria against oxidative stress. Collectively, the findings of this research suggest PCA is one of the key antimicrobial metabolites of bacterial strain YL-1, a promising biocontrol agent for disease management of BFB of cucurbit plants.

Screening of Antagonistic Bacterium to Control Konjac Soft Rot

2013 ◽  
Vol 726-731 ◽  
pp. 4427-4430
Author(s):  
Guo Hua Chen
Keyword(s):  
Bacterial Strain ◽  
Bacterial Pathogen ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Field Trials ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Plate Method ◽  
Antagonistic Bacterium

Konjac soft rot is a bottleneck limiting konjac yield caused by bacterial strain of Erwinia carotovora var. carotovora. In order to control konjac soft rot, soil samples were collected, and each sample was spread on surface of a plate seeded with E. carotovora var. carotovora in advance. Strains expressing antagonistic activities were selected and then isolated with streak plate method. One bacterial strain (named Z10) was obtained from soil by this method. In field trials, strain Z10 still showed antagonistic effect against the bacterial pathogen.

scholarly journals Biofilm Formation in Pseudomonas aeruginosa: Fimbrial cup Gene Clusters Are Controlled by the Transcriptional Regulator MvaT

2004 ◽  
Vol 186 (9) ◽  
pp. 2880-2890 ◽  
Author(s):  
Isabelle Vallet ◽  
Stephen P. Diggle ◽  
Rachael E. Stacey ◽  
Miguel Cámara ◽  
Isabelle Ventre ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Dna Microarrays ◽  
Transcriptional Profiling ◽  
Bacterial Pathogen ◽  
Gene Clusters ◽  
Northern Blotting ◽  
Negative Regulator ◽  
Regulatory Control ◽  
Homologous Gene

ABSTRACT Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical devices. Various stages of biofilm formation by this organism are mediated by extracellular appendages, such as type IV pili and flagella. Recently, we identified three P. aeruginosa gene clusters that were termed cup (chaperone-usher pathway) based on their sequence relatedness to the chaperone-usher fimbrial assembly pathway in other bacteria. The cupA gene cluster, but not the cupB or cupC cluster, is required for biofilm formation on abiotic surfaces. In this study, we identified a gene (mvaT) encoding a negative regulator of cupA expression. Such regulatory control was confirmed by several approaches, including lacZ transcriptional fusions, Northern blotting, and transcriptional profiling using DNA microarrays. MvaT also represses the expression of the cupB and cupC genes, although the extent of the regulatory effect is not as pronounced as with cupA. Consistent with this finding, mvaT mutants exhibit enhanced biofilm formation. Although the P. aeruginosa genome contains a highly homologous gene, mvaU, the repression of cupA genes is MvaT specific. Thus, MvaT appears to be an important regulatory component within a complex network that controls biofilm formation and maturation in P. aeruginosa.

scholarly journals Specificity and properties of the nucleotide carrier in chromaffin granules from bovine adrenal medulla

1983 ◽  
Vol 210 (3) ◽  
pp. 789-794 ◽  
Author(s):  
A Weber ◽  
E W Westhead ◽  
H Winkler
Keyword(s):  
Amino Acid ◽  
Carboxylic Acid ◽  
Pyridoxal Phosphate ◽  
Chromaffin Granules ◽  
Inhibitory Effects ◽  
Temperature Dependent ◽  
Arginine Residues ◽  
Disulphonic Acid ◽  
Important Position ◽  
Broad Specificity

1. The influence of various substances on the uptake of [3H]ATP and [14C]-noradrenaline into isolated bovine chromaffin granules was investigated. The carrier-mediated [3H]ATP uptake is specifically inhibited by SO42-, PO43- and phosphoenolpyruvate. Compounds with carboxylic acid or sulphonic acid groups had no significant inhibitory effects on either uptake. 2. 35SO42-, 32PO43- and phosphoenol[14C]pyruvate are taken up into chromaffin granules by a temperature-dependent process that is inhibited by atractyloside, uncouplers of oxidative phosphorylation and lipid-permeant anions. The apparent Km of 35SO42- uptake is 0.4 mM. 3. These results indicate that the nucleotide carrier in chromaffin granules has a broad specificity, transporting compounds with two strong negative charges. 4. Amino acid probes influence the uptake of ATP and catecholamines differently. Pyridoxal phosphate inhibits both uptake processes, 4,4′-di-isothiocyanostilbene-2,2′-disulphonic acid preferentially blocks ATP uptake, whereas phenylglyoxal blocks only ATP transport. It is suggested that the nucleotide carrier possesses arginine residues in a functionally important position. 5. The significance of these results obtained on isolated granules for the function of chromaffin granules within the cell is discussed.

scholarly journals Involvement of phenazine-1-carboxylic acid in the interaction between Pseudomonas chlororaphis subsp. aureofaciens strain M71 and Seiridium cardinale in vivo

2017 ◽  
Vol 199 ◽  
pp. 49-56 ◽  
Author(s):  
Aida Raio ◽  
Pierluigi Reveglia ◽  
Gerardo Puopolo ◽  
Alessio Cimmino ◽  
Roberto Danti ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Pseudomonas Chlororaphis ◽  
Seiridium Cardinale

scholarly journals Draft Genome Sequence of Endophytic BacteriumEnterobacter asburiaePDA134, Isolated from Date Palm (Phoenix dactyliferaL.) Roots

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Mahmoud W. Yaish
Keyword(s):  
Acetic Acid ◽  
Carboxylic Acid ◽  
Salinity Stress ◽  
Genome Sequence ◽  
Bacterial Strain ◽  
Date Palm ◽  
Draft Genome ◽  
Acc Deaminase ◽  
Enterobacter Asburiae ◽  
Indole 3 Acetic Acid

In this report, a draft of theEnterobacter asburiaestrain PDA134 genome was sequenced. This bacterial strain was isolated from the root tissue of a date palm, where it has the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and indole-3-acetic acid (IAA) under salinity stress.

scholarly journals Regulation of Ethylene Level in Mungbean (Vigna radiata L.) by 1-Aminocyclopropane-1-Carboxylic Acid (ACC)-Deaminase containing Bacterial Strain under Salt Stress

2016 ◽  
Vol 5 (11) ◽  
pp. 275-283
Author(s):  
Sarita Kumari ◽  
Anukool Vaishnav ◽  
Shekhar Jain ◽  
Devendra Kumar Choudhary ◽  
Kanti Prakash Sharma
Keyword(s):  
Salt Stress ◽  
Carboxylic Acid ◽  
Bacterial Strain ◽  
Vigna Radiata ◽  
Acc Deaminase ◽  
Ethylene Level

scholarly journals Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Nguyen Thi Trung ◽  
Nguyen Tien Cuong ◽  
Nguyen Thi Thao ◽  
Dao Thi Mai Anh ◽  
Do Thi Tuyen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Pseudomonas Aeruginosa ◽  
Antifungal Activity ◽  
Carboxylic Acid ◽  
Magnetic Resonance ◽  
13C Nmr ◽  
Bacterial Strain ◽  
Antifungal Compound ◽  
Fusarium Sp ◽  
High Antifungal Activity

Background: Fusarium sp. and Rhizoctonia sp. fungi have been always threats to short-term crops. In Vietnam, corn and soybean suffer serious losses annually. Therefore, it is necessary to utilize an environmentally friendly antifungal compound that is highly effective against phytopathogenic fungi. Pseudomonas sp. is a popular soil bacterial strain and well known for its high antifungal activity. Objectives: This study was carried out to evaluate and assess the antifungal activity of a local bacterial strain namely DA3.1 that was later identified as Pseudomonas aeruginosa. This would be strong scientific evidence to develop an environmentally friendly biocide from a local microorganism strain for commercial use. Methods: The antifungal compound was purified from ethyl acetate extraction of deproteinized cell culture broth by a silica gel column (CH2Cl2/MeOH (0% - 10% MeOH)). The purity of the isolated compound was determined by HPLC, and its molecular structure was elucidated using spectroscopic experiments including one-dimensional (1D) (1H NMR, 13C NMR, DEPT) and two-dimensional (2D) (HMBC and HSQC) spectra. The activity of the purified compound against Fusarium sp. and Rhizoctonia sp. fungi was measured using the PDA-disk diffusion method, and its growth-promoting ability was evaluated using the seed germination test of corn and soybean. Results: The results showed that the antifungal compound produced by Pseudomonas aeruginosa DA3.1 had a retention factor (Rf) of 0.86 on thin layer chromatography (TLC). Based on the evidence of spectral data including proton nuclear magnetic resonance (1H NMR), carbon nuclear magnetic resonance (13C NMR), distortionless enhancement by polarization transfer (DEPT), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum coherence (HSQC), the chemical structure was elucidated as phenazine-1-carboxylic. The purified compound showed inhibitory activity against F. oxysporum and R. solani and exhibited the ability of the germination of corn and soybean seeds. The results revealed the benefit of native P. aeruginosa DA3.1 and phenazine-1-carboxylic acid for use as a biocontrol agent, as well as a plant growth promoter. Conclusions: The antifungal compound isolated from local Pseudomonas DA3.1 was identified as phenazine-1-carboxylic acid that posed high antifungal activity and was a plant germination booster.

Phenazine-1-carboxylic acid biosynthesis in Pseudomonas Chlororaphis GP72 is positively regulated by the sigma factor RpoN

2008 ◽  
Vol 24 (9) ◽  
pp. 1961-1966 ◽  
Author(s):  
Hai-Ming Liu ◽  
An Yan ◽  
Xue-Hong Zhang ◽  
Yu-Quan Xu
Keyword(s):  
Carboxylic Acid ◽  
Sigma Factor ◽  
Pseudomonas Chlororaphis ◽  
Acid Biosynthesis ◽  
Pseudomonas Chlororaphis Gp72

Development of a TaqMan probe-based insulated isothermal PCR (TiiPCR) for the detection of Acidovorax citrulli, the bacterial pathogen of watermelon fruit blotch

2016 ◽  
Vol 147 (4) ◽  
pp. 869-875 ◽  
Author(s):  
Pei-Yi Wu ◽  
Lien-Chun Ho ◽  
Jun-Jie Chang ◽  
Kuo-Ching Tzeng ◽  
Wen-Ling Deng ◽  
...  
Keyword(s):  
Bacterial Pathogen ◽  
Taqman Probe ◽  
Acidovorax Citrulli ◽  
Insulated Isothermal Pcr
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