scholarly journals Carotenoid Cocktail Produced by An Antarctic Soil Flavobacterium with Biotechnological Potential

2021 ◽  
Vol 9 (12) ◽  
pp. 2419
Author(s):  
Paulina Pradel ◽  
Nancy Calisto ◽  
Laura Navarro ◽  
Andrés Barriga ◽  
Nicolás Vera ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Food Supplement ◽  
Luria Bertani ◽  
Main Peak ◽  
Marine Animal ◽  
Carotenoid Pigments ◽  
Antarctic Soil ◽  
16S Rrna Analysis ◽  
Pigment Extract

Carotenoids are highly important in pigmentation, and its content in farmed crustaceans and fish correlates to their market value. These pigments also have a nutritional role in aquaculture where they are routinely added as a marine animal food supplement to ensure fish development and health. However, there is little information about carotenoids obtained from Antarctic bacteria and its use for pigmentation improvement and flesh quality in aquaculture. This study identified carotenoids produced by Antarctic soil bacteria. The pigmented strain (CN7) was isolated on modified Luria–Bertani (LB) media and incubated at 4 °C. This Gram-negative bacillus was identified by 16S rRNA analysis as Flavobacterium segetis. Pigment extract characterization was performed through high-performance liquid chromatography (HPLC) and identification with liquid chromatography–mass spectrometry (LC–MS). HPLC analyses revealed that this bacterium produces several pigments in the carotenoid absorption range (six peaks). LC–MS confirms the presence of one main peak corresponding to lutein or zeaxanthin (an isomer of lutein) and several other carotenoid pigments and intermediaries in a lower quantity. Therefore, we propose CN7 strain as an alternative model to produce beneficial carotenoid pigments with potential nutritional applications in aquaculture.

scholarly journals Insight into the Metabolic Profiles of Pb(II) Removing Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4008
Author(s):  
Carla Cilliers ◽  
Evans M. N. Chirwa ◽  
Hendrik G. Brink
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Microbial Consortium ◽  
Luria Bertani ◽  
Nutrient Concentrations ◽  
Batch Experiments ◽  
Metabolic Study ◽  
Luria Bertani Broth ◽  
Tandem Mass Spectroscopy ◽  
Insight Into

The objective of the study was to gather insight into the metabolism of lead-removing microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a “non-targeted” fashion and high-performance liquid chromatography (HPLC) in a “targeted” fashion. Four main compounds were identified, and a metabolic study was conducted on each to establish their possible significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II) bioremoval, which in turn can result in a clarified understanding for development on an industrial and microbial level.

scholarly journals Cyclo(l-Pro–l-Leu) of Pseudomonas putida MCCC 1A00316 Isolated from Antarctic Soil: Identification and Characterization of Activity against Meloidogyne incognita

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 768 ◽  
Author(s):  
Yile Zhai ◽  
Zongze Shao ◽  
Minmin Cai ◽  
Longyu Zheng ◽  
Guangyu Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pseudomonas Putida ◽  
Meloidogyne Incognita ◽  
High Performance ◽  
Larval Mortality ◽  
Control Treatment ◽  
Hatching Rate ◽  
Antarctic Soil ◽  
Egg Hatching ◽  
Flight Mass Spectrometry

Pseudomonas putida MCCC 1A00316 was originally isolated from an Antarctic soil and has demonstrated potential nematicidal activity. Thus, it has promising applications for the biological control of Meloidogyne incognita. The larval mortality and egg-hatching inhibition rates of M. incognita will increase with the rising concentration of culture filtrates of P. putida MCCC 1A00316 and the duration of exposure. Thus, this study aimed to separate, purify, and identify nematicidal compounds from P. putida MCCC 1A00316 and to validate their anti-M. incognita activities. Compounds were purified through silica gel column chromatography and thin-layer chromatography combined with high-performance liquid chromatography (HPLC). Structural identification was conducted through liquid chromatography time-of-flight mass spectrometry, 1H nuclear magnetic resonance (NMR) spectroscopy, 13C-NMR, and Marfey’s method. The isolated compounds were identified as cyclo(l-Pro–l-Leu) on the basis of the results of the above analyses and previously reported data. The effects of various concentrations of cyclo(l-Pro–l-Leu) on the mortality rates of second-stage juveniles (J2) of M. incognita were investigated. Results showed that HPLC-purified cyclo(l-Pro–l-Leu) displayed nematicidal activities. The mortality rate of M. incognita J2 reached 84.3% after 72 h of exposure to 67.5 mg/L cyclo(l-Pro–l-Leu). The lowest egg-hatching rate (9.74%) was observed after 8 days of incubation with 2000 mg/L cyclo(l-Pro–l-Leu). An egg-hatching rate of 53.11% was obtained under the control treatment (sterile distilled water). However, cyclo(l-Pro–l-Leu) did not elicit chemotaxis activity to M. incognita. This is the first work to investigate the anti-M. incognita characteristics of cyclo(l-Pro–l-Leu).

scholarly journals Identification of Abies sibirica L. Polyprenols and Characterisation of Polyprenol-Containing Liposomes

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1801 ◽  
Author(s):  
Ilona Vanaga ◽  
Jerzy Gubernator ◽  
Ilva Nakurte ◽  
Ugis Kletnieks ◽  
Ruta Muceniece ◽  
...  
Keyword(s):  
High Performance ◽  
Food Supplement ◽  
Main Peak ◽  
Array Detector ◽  
Ethanol Injection ◽  
Abies Sibirica ◽  
Transmission Electron ◽  
Liposome Size ◽  
Commercial Applications ◽  
Conifer Trees

The needles of conifer trees are one of the richest sources of natural polyprenols. Polyprenol homologs from Abies sibirica L. lipophilic 80% purified extract were analyzed and quantified. In total, 10 peaks (Prenol-11 to Prenol-20) were observed in the ultra-high-performance liquid chromatography–diode array detector (UHPLC-DAD) chromatogram of Siberian fir with the most abundant compound being Prenol-15 (relative amount 37.23 + 0.56% of the total polyprenol yield). Abies sibirica L. polyprenol solubility and incorporation efficiency into liposomes were studied in various commercially available lecithin mixtures (Phosal IP40, Phosal 75SA, and Lipoid P45). The resulting multilamellar polyprenol liposomes were morphologically characterized by Light and Transmission Electron Microscopy, and the liposome size was discovered to be polymodal with the main peak at 1360 nm (90% of the volume). As polyprenols are fully soluble only in lipids, a liposomal formulation based upon co-solubilization and a modified ethanol injection method of polyprenols into the ethanol-phospholipid system was developed for the entrapment and delivery of polyprenols for potential commercial applications in food supplement and cosmetic industries.

Determination of vitamin E and carotenoid pigments by high performance liquid chromatography in shell of Chionoecetes opilio

2008 ◽  
Vol 617 (1-2) ◽  
pp. 225-229 ◽  
Author(s):  
María Vilasoa-Martínez ◽  
Carina Calaza-Ramos ◽  
Julia López-Hernández ◽  
María Asunción Lage-Yusty ◽  
Perfecto Paseiro Losada ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
High Performance ◽  
Carotenoid Pigments ◽  
Chionoecetes Opilio

scholarly journals Validation of method for the determination of bovine casein in milk and milk products

2018 ◽  
Vol 1 (1) ◽  
pp. 18-24
Author(s):  
Minh Hoa Nguyen Thi ◽  
Ha Binh Nguyen Thị ◽  
Son Tran Cao ◽  
◽  
◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Sandwich Elisa ◽  
Food Supplement ◽  
Elisa Method ◽  
Chromatography Method ◽  
Validation Data ◽  
Liquid Chromatography Method ◽  
Labeling Requirements

Bovine caseins in dairy and dairy products were determined by high performance liquid chromatography (HPLC) with a PDA detector at 280 nm or by sandwich ELISA method. Liquid chromatography method was developed to determine different types of casein including alpha, beta, and kappa casein at high content (% level), which calculate the proportion of casein forms, thus allow the identification of milk sources (cow's milk, goat's milk). The ELISA method determines casein at low content (LOD was of 3mg/kg) which meets the labeling requirements of EU and Japan for food allergens. The validation data show that both methods fit the purpose with the recovery of 78-98% and the coefficient of variation is from 2.4 to 9.5%. The methods have been applied for the determination of casein content in 20 samples of milk, food supplement, cake and candy from Hanois' markets.

scholarly journals Distribution of unique red feather pigments in parrots

2005 ◽  
Vol 1 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Kevin J McGraw ◽  
Mary C Nogare
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Carotenoid Pigments ◽  
Unique System ◽  
Feather Colouration

In many birds, red, orange and yellow feathers are coloured by carotenoid pigments, but parrots are an exception. For over a century, biochemists have known that parrots use an unusual set of pigments to produce their rainbow of plumage colours, but their biochemical identity has remained elusive until recently. Here, we use high-performance liquid chromatography to survey the pigments present in the red feathers of 44 species of parrots representing each of the three psittaciform families. We found that all species used the same suite of five polyenal lipochromes (or psittacofulvins) to colour their plumage red, indicating that this unique system of pigmentation is remarkably conserved evolutionarily in parrots. Species with redder feathers had higher concentrations of psittacofulvins in their plumage, but neither feather colouration nor historical relatedness predicted the ratios in which the different pigments appeared. These polyenes were absent from blood at the time when birds were replacing their colourful feathers, suggesting that parrots do not acquire red plumage pigments from the diet, but instead manufacture them endogenously at growing feathers.

scholarly journals Carob Seeds: Food Waste or Source of Bioactive Compounds?

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1090
Author(s):  
Debora Santonocito ◽  
Giuseppe Granata ◽  
Corrada Geraci ◽  
Annamaria Panico ◽  
Edy Angela Siciliano ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Bioactive Compounds ◽  
Food Waste ◽  
High Performance ◽  
Food Supplement ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Seed Extracts

(1) Background: For centuries, carob fruit has been used in the food field, while carob seeds have been mainly considered as food waste. Nowadays, there has been considerable attention toward the recovery of the waste plant matrices as possible sources of functional compounds with health properties. Therefore, our goal was to evaluate the health properties of carob seed extracts, and to study the effects of the ripening process on the chemical composition of the extracts. (2) Methods: After the mechanical separation of seeds from carob fruit, an ultrasound-assisted extraction (UAE) was performed to maximize and preserve the quality of bioactive compounds. Seed extracts were characterized by high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) for the content of bioactive polyphenols, and were finally analyzed by oxygen radical absorbance capacity (ORAC), NO Scavenger (NO) and advanced glyoxidation end products (AGEs) assays, in order to estimate the antioxidant potential of the active compounds. (3) Results: Although both seed extracts of carob unripe (CAR-UR) and ripe (CAR-R) showed an interesting antioxidant activity, CAR-R had greater activity due to the procyanidins content. (4) Conclusions: Based on the obtained results, carob seed extracts could be regarded as interesting source of bioactive antioxidant compounds for a potential application in nutraceutical and food supplement fields.

Sign in / Sign up

Export Citation Format

Share Document