scholarly journals Wild Rabbit Exposure to Leishmania infantum, Toxoplasma gondii, Anaplasma phagocytophilum and Babesia caballi Evidenced by Serum and Aqueous Humor Antibody Detection

2021 ◽  
Vol 9 (12) ◽  
pp. 2616
Author(s):  
Labrini V. Athanasiou ◽  
Eleni G. Katsogiannou ◽  
Constantina N. Tsokana ◽  
Sofia G. Boutsini ◽  
Marina G. Bisia ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Aqueous Humor ◽  
Anaplasma Phagocytophilum ◽  
Leishmania Infantum ◽  
Antibody Detection ◽  
Wild Rabbit ◽  
Likelihood Ratios ◽  
Hunting Season ◽  
Babesia Caballi ◽  
Zoonotic Pathogens

Wild rabbits (Oryctolagus cuniculus) can be important sentinel species for the presence of zoonotic pathogens. Therefore, we collected blood samples from wild rabbits harvested by hunters during the hunting season 2019–2020 on the island of Lemnos, to determine exposure of wild rabbits to the zoonotic pathogens Leishmania infantum, Toxoplasma gondii, Anaplasma phagocytophilum and Babesia caballi, as well as aqueous humor to assess its diagnostic performance in terms of sensitivity, specificity, positive and negative likelihood ratios. Antibodies against these pathogens were detected by Indirect Immunofluorescence Antibody (IFA) assay. Out of the 72 wild rabbits included in the study, 4.2%, 5.5%, 18% and 9.7% were seropositive to L. infantum, T. gondii, A. phagocytophilum and B. caballi, respectively. Although less frequently, antibodies were also detected in aqueous humor of wild rabbits. The antibody detection in aqueous humor presented 100% specificity but decreased sensitivity compared to serum suggesting that aqueous humor could be successfully used in epidemiological studies to confirm exposure at the population level but has little diagnostic value at the individual level. This is the first report on the seropositivity of wild rabbits to A. phagocytophilum and B. caballi and the detection of antibodies against A. phagocytopylum, L. infantum, T. gondii and B. caballi in the aqueous humor.

Leishmania infantum and Toxoplasma gondii: Mixed infection of macrophages in vitro and in vivo

2011 ◽  
Vol 128 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Vasiliki Christodoulou ◽  
Ippokratis Messaritakis ◽  
Eleni Svirinaki ◽  
Christos Tsatsanis ◽  
Maria Antoniou
Keyword(s):  
Toxoplasma Gondii ◽  
Mixed Infection ◽  
Leishmania Infantum

Kennel dogs as sentinels of Leishmania infantum, Toxoplasma gondii, and Neospora caninum in Majorca Island, Spain

2010 ◽  
Vol 107 (6) ◽  
pp. 1505-1508 ◽  
Author(s):  
Oscar Cabezón ◽  
Javier Millán ◽  
Margalida Gomis ◽  
Jitender P. Dubey ◽  
Ezio Ferroglio ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Neospora Caninum

scholarly journals Presence of antibodies against Toxoplasma gondii, Neospora caninum and Leishmania infantum in dogs from Piauí

2011 ◽  
Vol 20 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Marcos Gomes Lopes ◽  
Ivete Lopes Mendonça ◽  
Kleber Portela Fortes ◽  
Marcos Amaku ◽  
Hilda de Fátima Jesus Pena ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Northeastern Brazil ◽  
Leishmania Infection ◽  
Serum Samples ◽  
Chi Square ◽  
Leishmania Spp ◽  
And Gender

This study aimed to evaluate the presence of antibodies against Neospora caninum, Toxoplasma gondii and Leishmania infantum in dogs attended at the Veterinary Hospital of the Federal University of Piauí, Northeastern Brazil, where there are no reports of the occurrence of N. caninum and T. gondii in dogs. Serum samples from 530 dogs of genders, different ages and breeds from the municipality of Teresina and nearby towns were analyzed using three indirect fluorescent antibody tests, each one targeting one of the three agents. The associations between the parasites and gender, breed and age of the dogs were assessed by the chi-square test (p > 0.05). The occurrence of antibodies to N. caninum, T. gondii and L. infantum was 3.2, 18.0 and 78.1%, respectively. Toxoplasma gondii was more frequently found in older dogs (p < 0.05) whereas L. infantum was more common in animals aged between 1 to 3 years (p < 0.05). In order to evaluate potential associations between the presence of anti-N. caninum and anti-T. gondii antibodies and Leishmania infection, 240 dogs were selected (120 positive and 120 negative for Leishmania spp.), based on serological and parasitological diagnoses. No association was found between Leishmania spp. and the coccidian parasites (p > 0.05). The results confirm the exposure of dogs to these parasites in the State of Piauí.

scholarly journals PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

2020 ◽  
Vol 8 (12) ◽  
pp. 1926
Author(s):  
Catarina Coelho ◽  
Madalena Vieira-Pinto ◽  
Anabela Vilares ◽  
Maria João Gargaté ◽  
Manuela Rodrigues ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Oryctolagus Cuniculus ◽  
Wild Rabbit ◽  
Human Beings ◽  
Source Of Infection ◽  
Zoonotic Parasite ◽  
Central Portugal ◽  
Sag2 Gene ◽  
Rabbit Meat ◽  
Meat Samples

Wildlife plays an important role in the epidemiological cycle of Toxoplasma gondii. The European wild rabbit (Oryctolagus cuniculus) can be a source of infection to wild and domestic hosts, including human beings. Additionally, as an herbivorous animal, the European wild rabbit may also be a sentinel of environmental contamination with T. gondii and, consequently, an indicator of the potential transmission of this parasite. The purpose of the present work was to detect T. gondii DNA in European wild rabbit from central Portugal, as well as the possible implications for public health. Heart and diaphragm samples were obtained from 28 rabbits hunted in central Portugal. Nested PCR separately amplified the 5′ and 3′ ends of the surface antigen 2 (SAG2) gene. T. gondii DNA was detected in 19 out of the 28 sampled animals, resulting in a prevalence of 67.9%. These results show that T. gondii infection occurs in European wild rabbit and therefore may pose a potential risk for humans if consumed as raw or undercooked meat. Measures should be taken in order to prevent infection by this zoonotic parasite and for the conservation of wildlife. To the best of our knowledge, this is the first study performed by means of PCR on T. gondii in European wild rabbit meat samples.

scholarly journals Prevalence and genetic characterization of Toxoplasma gondii in naturally infected backyard pigs intended for familial consumption in Romania

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Anamaria Ioana Paştiu ◽  
Anamaria Cozma-Petruț ◽  
Aurélien Mercier ◽  
Anamaria Balea ◽  
Lokman Galal ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Antibody Detection ◽  
Serum Samples ◽  
Potential Threat ◽  
Polymerase Chain ◽  
Pcr Targeting ◽  
Backyard Pigs

Abstract Background Foodborne toxoplasmosis in humans can be due to the exposure to tissue cysts of Toxoplasma gondii through the consumption of meat, including pork, of infected animals. Traditional Romanian food habits include pork as the preferred meat, while backyard pig rearing remains a common practice in many rural areas of Romania. The aims of the present study were to estimate the prevalence of T. gondii infection in naturally infected backyard pigs slaughtered for familial consumption and to genetically characterize the T. gondii strains obtained. Methods Paired blood and heart samples were collected from 94 backyard pigs, home slaughtered for private consumption. Serum samples were analyzed using the immunofluorescence antibody test (IFAT) for anti-T. gondii antibody detection. Heart samples were screened by polymerase chain reaction (PCR) targeting the 529-bp repeat region (REP529) for T. gondii detection. In addition, heart samples from IFAT positive animals were bioassayed in mice. The T. gondii isolates were genotyped by the analysis of 15 microsatellite markers. Results The results showed that almost half of the pigs investigated were T. gondii seropositive (46.8%, 95% confidence interval (CI): 36.4–57.4%) and in more than a quarter of the pigs (26.6%, 95% CI: 18.0–36.7%), the parasite was detected by PCR. Three (3/44) T. gondii strains were isolated from hearts of seropositive pigs and they all belonged to genotype II. Conclusions The present study showed the presence of T. gondii infection in backyard pigs in Romania, which suggests that consumption of pork from animals reared and slaughtered at home may pose a potential threat to human health and should be given attention. In addition, to our knowledge, this is the first study to provide data concerning T. gondii strains circulating in pigs from Romania.

scholarly journals Heat treatment alleviates the growth and photosynthetic impairment of transplastomic plants expressing Leishmania infantum Hsp83-Toxoplasma gondii SAG1 fusion protein

Plant Science ◽  
2019 ◽  
Vol 284 ◽  
pp. 117-126 ◽  
Author(s):  
Mariana G. Corigliano ◽  
Romina M. Albarracín ◽  
Juan M. Vilas ◽  
Edwin F. Sánchez López ◽  
Sofía A. Bengoa Luoni ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Toxoplasma Gondii ◽  
Fusion Protein ◽  
Leishmania Infantum ◽  
Transplastomic Plants

scholarly journals Immune Responses of Iranian Patients with Visceral Leishmaniasis and Recovered Individuals to LCR1 of Leishmania infantum

2014 ◽  
Vol 21 (4) ◽  
pp. 518-525 ◽  
Author(s):  
Hamid M. Niknam ◽  
Firoozeh Abrishami ◽  
Mohammad Doroudian ◽  
Mosayeb Rostamian ◽  
Maryam Moradi ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Immune Responses ◽  
Leishmania Infantum ◽  
Mononuclear Cells ◽  
Antibody Responses ◽  
Antibody Detection ◽  
Recent History ◽  
Content Type ◽  
History Of ◽  
Ifn Γ

ABSTRACTVisceral leishmaniasis is a serious public health problem.Leishmania infantumis one of its causative agents. LCR1 is an immunogen fromL. infantum. Antibodies against this protein have been detected in visceral leishmaniasis patients. The aim of this study was to define the antibody and cellular immune responses against LCR1 in Iranian visceral leishmaniasis patients and recovered individuals. The LCR1 protein was produced in recombinant form. Antibody responses against this protein were studied in Iranian individuals with a recent history of visceral leishmaniasis. Responses of peripheral blood mononuclear cells to this protein were studied in Iranian individuals who had recovered from visceral leishmaniasis. Our data show that (i) there was an antibody response to LCR1 in each individual with a recent history of visceral leishmaniasis studied, (ii) there was neither a proliferative response nor production of gamma interferon (IFN-γ) or interleukin 10 in response to LCR1 by mononuclear cells from individuals who had recovered from visceral leishmaniasis, and (iii) individuals who have recovered from visceral leishmaniasis show ongoing immune responses long after recovery from the disease. These data show that there are no detectable cellular memory responses to LCR1 in Iranian individuals who have recovered from visceral leishmaniasis, while there are detectable antibody responses in patients with this disease. Our data suggest that LCR1 has potential applications for the diagnosis of leishmaniasis through antibody detection, while the application of LCR1 alone for induction of IFN-γ in individuals who recovered from this disease is not supported. The presence of long-lasting immune reactivities in individuals who recovered from the disease may show the necessity of extended medical surveillance for these individuals.

scholarly journals FLUORESCENT ANTIBODY DETECTION OF THE ANTIGENS OF THE SHOPE PAPILLOMA VIRUS IN PAPILLOMAS OF THE WILD AND DOMESTIC RABBIT

1957 ◽  
Vol 106 (4) ◽  
pp. 555-562 ◽  
Author(s):  
Wilbur Fiske Noyes ◽  
Robert C. Mellors
Keyword(s):  
Nucleic Acid ◽  
Early Stage ◽  
Fluorescent Antibody ◽  
Antibody Detection ◽  
Wild Rabbit ◽  
Papilloma Virus ◽  
Viral Antigens ◽  
Stage Of Development ◽  
Domestic Rabbit ◽  
Shope Papilloma Virus

The results obtained by a fluorescent antibody study of the Shope papilloma virus in papillomas of the wild and domestic rabbit are presented. In the wild rabbit papillomas the viral antigens occurred exclusively in the nucleus and were present in the differentiating cells of the keratohyaline layers and in the keratinized layers. The antigens were not present in the deeper proliferating epithelial cells of the papillomas. The Shope viral antigens were present in very minute amounts in papillomas of the domestic rabbit, as compared with papillomas of the wild rabbit, and were only detected in the superficial keratinized layers. It is postulated that virus is present in the nuclei of the proliferating cells of the papillomas of the wild and domestic rabbit but exists there in an early stage of development, consisting mainly of nucleic acid and deficient in protein, therefore non-antigenic and not demonstrable by fluorescent antibody. The nucleic acid moiety of the virus may be infective, and the protein component may provide immunologic specificity and serve to preserve transmissibility. The protein-deficient virus can be referred to as masked virus.

scholarly journals Seroprevalence, spatial distribution and risk factors associated with Toxoplasma gondii infection among cattle in a quilombola community in the Brazilian cerrado

2021 ◽  
Vol 30 (1) ◽  
Author(s):  
Daniella Ferreira Cordeiro Gomes ◽  
Lucas Andrade Mendes ◽  
Juliana Moraes Dias ◽  
Müller Ribeiro-Andrade ◽  
Pollyanne Raysa Fernandes de Oliveira ◽  
...  
Keyword(s):  
Risk Factors ◽  
Spatial Distribution ◽  
Toxoplasma Gondii ◽  
Univariate Analysis ◽  
Antibody Test ◽  
Antibody Detection ◽  
Occurrence Rate ◽  
Factors Associated ◽  
Average Annual Temperature ◽  
Toxoplasma Gondii Infection

Abstract Little is known about Toxoplasma gondii infection among cattle living in the Cerrado (Brazilian savanna) biome in Brazil. In particular, there is no epidemiological data relating to infection in quilombo lands, i.e. areas settled by Afro-descendants of escaped slaves. The aim of this study was to determine the prevalence, spatial distribution and risk factors associated with T. gondii infection among cattle in the Kalunga quilombo, in the Cerrado biome. Blood samples were collected from 1533 cattle for antibody detection using the indirect fluorescence antibody test (IFAT). The study area was subdivided into five macroregions to determine the spatial distribution of infection. An objective questionnaire was applied to the cattle owners to evaluate risk factors, which were analyzed using univariate analysis and logistic regression. The prevalence of T. gondii infection among cattle was 8.93% (137/1533), and antibodies were found in 49.6% of the herds (66/133), in all macroregions. The risk factors associated with T. gondii infection in cattle were the following: number of animals in the herd (OR: 30.56), purchase of cattle (OR: 2.57), age group (OR: 1.95) and average annual temperature (OR: 1.77). Thus, the occurrence rate, spatial distribution and risk factors associated with T. gondii infection among cattle in the Kalunga quilombola community are documented here, for the first time.

scholarly journals Hemoparasites and Antibody Detection against Toxoplasma gondii and Neospora caninum in Dogs and Cats in Roraima State

2019 ◽  
Vol 13 (4) ◽  
Author(s):  
Marco Antonio Ritter Bastos Gomes ◽  
Paulo Roberto Spiller ◽  
Fernando Weibe Ferreira de Paiva ◽  
Taiã Mairon Peixoto Ribeiro
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Detection
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