scholarly journals Testing the Capacity of Staphylococcus equorum for Calcium and Copper Removal through MICP Process

Minerals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 905
Author(s):  
Sebastián Sepúlveda ◽  
Carla Duarte-Nass ◽  
Mariella Rivas ◽  
Laura Azócar ◽  
Andrés Ramírez ◽  
...  
Keyword(s):  
Urease Activity ◽  
Culture Conditions ◽  
Copper Tolerance ◽  
Process Water ◽  
Copper Removal ◽  
Calcium Carbonate Precipitation ◽  
Mining Operations ◽  
Removal Capacity ◽  
Ammonia Complex ◽  
Staphylococcus Equorum

This research focused on the evaluation of the potential use of a soil-isolated bacteria, identified as Staphylococcus equorum, for microbial-induced calcite precipitation (MICP) and copper removal. Isolated bacteria were characterized considering growth rate, urease activity, calcium carbonate precipitation, copper tolerance as minimum inhibitory concentration (MIC) and copper precipitation. Results were compared with Sporosarcina pasteurii, which is considered a model bacteria strain for MICP processes. The results indicated that the S. equorum strain had lower urease activity, calcium removal capacity and copper tolerance than the S. pasteurii strain. However, the culture conditions tested in this study did not consider the halophilic feature of the S. equorum, which could make it a promising bacterial strain to be applied in process water from mining operations when seawater is used as process water. On the other hand, copper removal was insufficient when applying any of the bacteria strains evaluated, most likely due to the formation of a copper–ammonia complex. Thus, the implementation of S. equorum for copper removal needs to be further studied, considering the optimization of culture conditions, which may promote better performance when considering calcium, copper or other metals precipitation.

Study on the mechanism of copper–ammonia complex decomposition in struvite formation process and enhanced ammonia and copper removal

2017 ◽  
Vol 51 ◽  
pp. 222-233 ◽  
Author(s):  
Cong Peng ◽  
Liyuan Chai ◽  
Chongjian Tang ◽  
Xiaobo Min ◽  
Yuxia Song ◽  
...  
Keyword(s):  
Formation Process ◽  
Copper Removal ◽  
Ammonia Complex

scholarly journals Influence of Substrate Mineralogy on Bacterial Mineralization of Calcium Carbonate: Implications for Stone Conservation

2012 ◽  
Vol 78 (11) ◽  
pp. 4017-4029 ◽  
Author(s):  
Carlos Rodriguez-Navarro ◽  
Fadwa Jroundi ◽  
Mara Schiro ◽  
Encarnación Ruiz-Agudo ◽  
María Teresa González-Muñoz
Keyword(s):  
Calcium Carbonate ◽  
Culture Conditions ◽  
Building Stone ◽  
Bacterial Attachment ◽  
Bacterial Cells ◽  
Calcium Carbonate Precipitation ◽  
Stone Conservation ◽  
Historical Building ◽  
Content Type ◽  
Polymorph Selection

ABSTRACTThe influence of mineral substrate composition and structure on bacterial calcium carbonate productivity and polymorph selection was studied. Bacterial calcium carbonate precipitation occurred on calcitic (Iceland spar single crystals, marble, and porous limestone) and silicate (glass coverslips, porous sintered glass, and quartz sandstone) substrates following culturing in liquid medium (M-3P) inoculated with different types of bacteria (Myxococcus xanthus,Brevundimonas diminuta, and a carbonatogenic bacterial community isolated from porous calcarenite stone in a historical building) and direct application of sterile M-3P medium to limestone and sandstone with their own bacterial communities. Field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), powder X-ray diffraction (XRD), and 2-dimensional XRD (2D-XRD) analyses revealed that abundant highly oriented calcite crystals formed homoepitaxially on the calcitic substrates, irrespective of the bacterial type. Conversely, scattered spheroidal vaterite entombing bacterial cells formed on the silicate substrates. These results show that carbonate phase selection is not strain specific and that under equal culture conditions, the substrate type is the overruling factor for calcium carbonate polymorph selection. Furthermore, carbonate productivity is strongly dependent on the mineralogy of the substrate. Calcitic substrates offer a higher affinity for bacterial attachment than silicate substrates, thereby fostering bacterial growth and metabolic activity, resulting in higher production of calcium carbonate cement. Bacterial calcite grows coherently over the calcitic substrate and is therefore more chemically and mechanically stable than metastable vaterite, which formed incoherently on the silicate substrates. The implications of these results for technological applications of bacterial carbonatogenesis, including building stone conservation, are discussed.

scholarly journals UTILIZAÇÃO DE BIOSSORVENTE NO TRATAMENTO DE ÁGUAS RESIDUÁRIAS RICAS EM COBRE (II) E SUA REUTILIZAÇÃO NA PRODUÇÃO DE ALFACE

Irriga ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 155-170
Author(s):  
Mara Lúcia Cruz de Souza ◽  
José Antonio Rodrigues de Souza ◽  
DÉBORA ASTONI MOREIRA ◽  
JOÃO DE JESUS GUIMARÃES ◽  
ELLEN LEMES SILVA ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Adsorption Isotherm ◽  
Low Cost ◽  
Langmuir Adsorption Isotherm ◽  
Copper Removal ◽  
Langmuir Adsorption ◽  
Removal Capacity ◽  
High Copper ◽  
Egg Shell

UTILIZAÇÃO DE BIOSSORVENTE NO TRATAMENTO DE ÁGUAS RESIDUÁRIAS RICAS EM COBRE (II) E SUA REUTILIZAÇÃO NA PRODUÇÃO DE ALFACE     MARA LÚCIA CRUZ DE SOUZA1; JOSÉ ANTONIO RODRIGUES DE SOUZA 2; DÉBORA ASTONI MOREIRA3; JOÃO DE JESUS GUIMARÃES 4; ELLEN LEMES SILVA 5 E JOÃO VICTOR COSTA6   Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas – FCA – Unesp, Câmpus Botucatu, Avenida Universitária, 3780, CEP 18610-034, Altos do Paraíso, Botucatu/SP, Brasil, [email protected] Departamento de Engenharia Agrícola, Instituto Federal de Educação Ciência e Tecnologia Goiano-Campus Urutaí, Rodovia Geraldo Silva Nascimento Km 2,5, Zona Rural, Urutaí-GO, Brasil, [email protected] Departamento de Engenharia Agrícola, Instituto Federal de Educação Ciência e Tecnologia Goiano-Campus Urutaí, Rodovia Geraldo Silva Nascimento Km 2,5, Zona Rural, Urutaí-GO, Brasil, [email protected] Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas – FCA – Unesp, Câmpus Botucatu, Avenida Universitária, 3780, CEP 18610-034, Altos do Paraíso, Botucatu/SP, Brasil, [email protected] Departamento de Recursos Naturais do Cerrado, Instituto Federal de Educação Ciência e Tecnologia Goiano-Campus Urutaí, Rodovia Geraldo Silva Nascimento Km 2,5, Zona Rural, Urutaí-GO, Brasil, [email protected] Instituto Federal de Educação Ciência e Tecnologia Goiano-Campus Urutaí, Rodovia Geraldo Silva Nascimento Km2.5, Zona Rural, Urutaí-GO, Brasil, [email protected]     1 RESUMO   Neste trabalho objetivou-se, estudar o tratamento de águas residuárias ricas em cobre por meio de biossorção, bem como o posterior reuso deste biossorvente na produção de alface. Para isso, avaliou-se a capacidade de remoção do cobre (II) pela casca de ovo moída por meio da isoterma de adsorção de Langmuir. Posteriormente, para estudar os efeitos do reuso deste adsorvente, diferentes doses foram adicionadas a vasos cultivados com alface, avaliando-se os efeitos no solo e na planta. Os resultados permitiram concluir que a casca de ovo moída apresentou elevada capacidade de remoção do cobre (25,4291 mg g-1), não havendo contaminação do solo e as plantas de alface apresentaram-se aptas para o consumo. Assim, a bioadssorção e o posterior reuso do adsorvente na produção de alface mostrou-se uma técnica ambientalmente adequada e de baixo custo. Palavras-chave: reuso, bioadssorvente, metal pesado.     SOUZA, M. L. C.1; SOUZA, J. A. R.2; MOREIRA, D. A.3; GUIMARÃES, J. J.4; SILVA, E. L.5; COSTA, J. V.6 USE OF BIOSORBENTS IN THE TREATMENT OF WASTEWATER RICH IN COPPER (II) AND THEIR REUSE ON LETTUCE PRODUCTION         2 ABSTRACT   The present work aimed at studying the treatment of wastewater rich in copper by biosorption, and the subsequent reuse of this biosorbent in the production of lettuce. For this, the capacity of copper (II) removal by the egg shell ground by means of the Langmuir adsorption isotherm was evaluated. Subsequently, the effects of reuse of this adsorbent was studied, and different concentrations were added to pots grown with lettuce, evaluating the effects on soil and plant. The results showed that the egg shell ground presented high copper removal capacity (25.4291 mg g-1), with no contamination of the soil and the lettuce plants were suitable for consumption. Thus, biosorption and subsequent reuse of the adsorbent in the production of lettuce proved to be an environmentally adequate and low-cost technique.   Keywords: reuse, biosorbent, heavy metal.

scholarly journals Synthesis of Zeolites from Greek Fly Ash and Assessment of Their Copper Removal Capacity

Minerals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 844
Author(s):  
Aikaterini Vavouraki ◽  
Georgios Bartzas ◽  
Konstantinos Komnitsas
Keyword(s):  
Fly Ash ◽  
Removal Efficiency ◽  
Analytical Techniques ◽  
Copper Removal ◽  
Concerted Action ◽  
Xps Spectra ◽  
Removal Capacity ◽  
Lignite Fly Ash ◽  
Pseudo Second Order ◽  
Second Order Equations

The objective of this study was to synthesize zeolites through fusion of lignite fly ash and NaOH or KOH pellets at 600 °C and assess their removal efficiency in terms of decontamination of solutions containing Cu(II) ions. The removal efficiency of the produced zeolites was tested in batch kinetic experiments using different zeolites and Cu(II) ion concentrations. Experimental data revealed that zeolites synthesized with the use of NaOH exhibited higher removal efficiency compared with those synthesized with the use of KOH. Kinetic data showed that the pseudo-second-order equations described well the removal process. Copper removal was mainly accomplished through the concerted action of chemisorption and intraparticle diffusion. Analytical techniques involving XRF, XRD, FTIR, SEM/EDS and XPS were used for the characterization and morphology analysis of the produced zeolites. SEM/EDS confirmed the presence of copper on the zeolite surface. XPS spectra of Cu2p at 934.3 eV proved the presence of Cu(II) oxidation state, confirming the possible formation of CuO and/or Cu-Cl.

Influence of feedstock on the copper removal capacity of waste-derived biochars

2016 ◽  
Vol 212 ◽  
pp. 199-206 ◽  
Author(s):  
Diego Arán ◽  
Juan Antelo ◽  
Sarah Fiol ◽  
Felipe Macías
Keyword(s):  
Copper Removal ◽  
Removal Capacity

A preliminary study of improving the MICP effects via the extractions from seashell nacre

2022 ◽  
Vol 12 (1) ◽  
pp. 1-20
Author(s):  
X. Xu ◽  
H. Guo ◽  
M. Li ◽  
H. Fu
Keyword(s):  
Mechanical Properties ◽  
Urease Activity ◽  
Water Soluble ◽  
Utilization Rate ◽  
Calcium Carbonate Precipitation ◽  
Average Strength ◽  
Sem Images ◽  
Cemented Sand ◽  
Future Studies ◽  
Preliminary Study

In microbially induced calcium carbonate precipitation (MICP) process, it is the precipitated CaCO3 that cements loose sand particles together to improve their mechanical properties. Seashell nacre composed of CaCO3 is a natural product, which is worth researching for its great hardness, strength, and toughness. However, there is no study connecting this natural nacre mineralization with MICP. Therefore, a precedent herein is established to modify the MICP process via the water-soluble matrix (WSM) extracted from nacre, where WSM contributes to the great mechanical properties of nacre. Correspondingly, this study examines the effects of WSM with different concentrations on urease activity and strength as well as microstructure of bio-cemented sand samples. The experimental results show that a small number of WSM (50mg/L) can improve the average strength of bio-cemented sand samples 1.5 times. This is because 50mg/L WSM can significantly improve the urease activity of bacteria meanwhile increasing the Ca2+ utilization rate. Thus, more CaCO3 crystals are precipitated, and the higher UCS of bio-cemented sand samples is achieved. Moreover, the XRD results indicate that the precipitated CaCO3 is almost calcite, and only a little aragonite is detected when the concentration of WSM increases to 100mg/L. Additionally, the SEM images demonstrate that WSM involvement can affect the shapes and sizes of CaCO3 crystals. Overall, this work is an unprecedented exploration imitating nacre that hopefully paves way for future studies.

scholarly journals STUDY ON DIFFERENT FACTORS AFFECT COD REMOVAL CAPACITY OF HALANAEROBIUM LACRUISEI IN SALINE WASTEWATER

2020 ◽  
Vol 58 (5A) ◽  
pp. 180
Author(s):  
Nguyen Thuy Chung
Keyword(s):  
Culture Conditions ◽  
Laboratory Method ◽  
Cod Removal ◽  
Effluent Treatment ◽  
Processing Plant ◽  
Saline Wastewater ◽  
Treatment Area ◽  
Removal Capacity ◽  
Number Of Factors ◽  
Environmental Requirements

This study evaluated a number of factors (including temperature, pH, salinity) affecting the growth of Halanaerobium lacruisei bacteria in order to reduce COD concentration in saline wastewater of a fish sauce processing plant. The results of the study showed that halophillic bacteria had good capacity of reducing COD concentration under specific conditions.Laboratory experiments with different bacterial culture conditions showed that 30°C, pH 7.0 and 6-7 % salinity is the optimal culturing condition for Halanaerobium lacruisei treating COD in wastewater. In the laboratory condition, COD treatment capacity in saline wastewater of this microorganism can be up to nearly 88% after 48 hours, ensuring that the effluent meets the environmental requirements. In pilot condition of culturing the bacteria (in plastic tank, stimulating the real condition), after 25 days, the COD removal decreased continously, 80% effectively compared with the inlet wastewater. The effluent treatment efficiency by a laboratory method meets the standard of type B wastewater specified in QCVN 40:2011/BTNMT. This study showed that Halanaerobium lacruisei can be usedeffectively to treat the COD parameter in saline wastewater which can be applied in industrial wastewater treatment area as well.

Screening of copper tolerance fungi strains and their characteristics for copper removal

2014 ◽  
Vol 39 (6) ◽  
pp. 670-674
Author(s):  
Li-kun XIE ◽  
Jing-an WANG ◽  
Zheng-guo SONG ◽  
Zhong-qi LIU
Keyword(s):  
Copper Tolerance ◽  
Copper Removal
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