scholarly journals Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories

Molecules ◽  
2016 ◽  
Vol 21 (2) ◽  
pp. 182 ◽  
Author(s):  
Karla Ramirez-Estrada ◽  
Heriberto Vidal-Limon ◽  
Diego Hidalgo ◽  
Elisabeth Moyano ◽  
Marta Golenioswki ◽  
...  
Keyword(s):  
Plant Cell ◽  
Added Value ◽  
Effective Strategy ◽  
Biotechnological Production ◽  
Cell Factories

scholarly journals Plasmid Replicons for the Production of Pharmaceutical-Grade pDNA, Proteins and Antigens by Lactococcus lactis Cell Factories

2021 ◽  
Vol 22 (3) ◽  
pp. 1379
Author(s):  
Sofia O.D. Duarte ◽  
Gabriel A. Monteiro
Keyword(s):  
Lactococcus Lactis ◽  
Recombinant Proteins ◽  
Plasmid Copy Number ◽  
Fermented Food ◽  
Added Value ◽  
Regulatory Sequences ◽  
Plasmid Copy ◽  
Mucosal Vaccination ◽  
Cell Factories ◽  
Plasmid Replicons

The Lactococcus lactis bacterium found in different natural environments is traditionally associated with the fermented food industry. But recently, its applications have been spreading to the pharmaceutical industry, which has exploited its probiotic characteristics and is moving towards its use as cell factories for the production of added-value recombinant proteins and plasmid DNA (pDNA) for DNA vaccination, as a safer and industrially profitable alternative to the traditional Escherichia coli host. Additionally, due to its food-grade and generally recognized safe status, there have been an increasing number of studies about its use in live mucosal vaccination. In this review, we critically systematize the plasmid replicons available for the production of pharmaceutical-grade pDNA and recombinant proteins by L. lactis. A plasmid vector is an easily customized component when the goal is to engineer bacteria in order to produce a heterologous compound in industrially significant amounts, as an alternative to genomic DNA modifications. The additional burden to the cell depends on plasmid copy number and on the expression level, targeting location and type of protein expressed. For live mucosal vaccination applications, besides the presence of the necessary regulatory sequences, it is imperative that cells produce the antigen of interest in sufficient yields. The cell wall anchored antigens had shown more promising results in live mucosal vaccination studies, when compared with intracellular or secreted antigens. On the other side, engineering L. lactis to express membrane proteins, especially if they have a eukaryotic background, increases the overall cellular burden. The different alternative replicons for live mucosal vaccination, using L. lactis as the DNA vaccine carrier or the antigen producer, are critically reviewed, as a starting platform to choose or engineer the best vector for each application.

scholarly journals Novel Insights into the Biotechnological Production of Haematococcus pluvialis-Derived Astaxanthin: Advances and Key Challenges to Allow Its Industrial Use as Novel Food Ingredient

2020 ◽  
Vol 8 (10) ◽  
pp. 789 ◽  
Author(s):  
Samuel Jannel ◽  
Yanis Caro ◽  
Marc Bermudes ◽  
Thomas Petit
Keyword(s):  
Haematococcus Pluvialis ◽  
Biological Activities ◽  
Biochemical Properties ◽  
Added Value ◽  
Human Consumption ◽  
Biotechnological Production ◽  
Food Ingredient ◽  
Industrial Sustainability ◽  
Natural Forms ◽  
Biotechnological Processes

Astaxanthin shows many biological activities. It has acquired a high economic potential and its current market is dominated by its synthetic form. However, due to the increase of the health and environmental concerns from consumers, natural forms are now preferred for human consumption. Haematococcus pluvialis is artificially cultured at an industrial scale to produce astaxanthin used as a dietary supplement. However, due to the high cost of its cultivation and its relatively low biomass and pigment productivities, the astaxanthin extracted from this microalga remains expensive and this has probably the consequence of slowing down its economic development in the lower added-value market such as food ingredient. In this review, we first aim to provide an overview of the chemical and biochemical properties of astaxanthin, as well as of its natural sources. We discuss its bioavailability, metabolism, and biological activities. We present a state-of-the-art of the biology and physiology of H. pluvialis, and highlight novel insights into the biotechnological processes which allow optimizing the biomass and astaxanthin productivities. We are trying to identify some lines of research that would improve the industrial sustainability and economic viability of this bio-production and to broaden the commercial potential of astaxanthin produced from H. pluvialis.

High-Added-Value Antioxidants Obtained from the Degradation of Wine Phenolics by Lactobacillus plantarum

2007 ◽  
Vol 70 (11) ◽  
pp. 2670-2675 ◽  
Author(s):  
JOSÉ MARÍA LANDETE ◽  
HÉCTOR RODRÍGUEZ ◽  
BLANCA DE LAS RIVAS ◽  
ROSARIO MUÑOZ
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Lactobacillus Plantarum ◽  
Antimicrobial Activities ◽  
Added Value ◽  
Methyl Gallate ◽  
Biotechnological Production ◽  
Wine Production ◽  
High Concentrations ◽  
Ferulic Acids

Disposal of the waste from wine production has long been a problem for wineries, mainly because of the presence of phenolic compounds. In this study, we analyzed the antimicrobial activities of 10 wine phenolic compounds against Lactobacillus plantarum strains. Inhibition increased in this order: catechin = gallic acid < epicatechin = salicylic acid < methyl gallate = caffeic acid < ferulic acid = tryptophol < p-coumaric acid. The obtained results indicated that L. plantarum is able to grow in the presence of high concentrations of some wine phenolic compounds. Of the 10 compounds analyzed, only the hydroxycinnamic acids, gallic acid, and methyl gallate were metabolized by the four L. plantarum strains studied. Results also revealed that 4-vinylphenol and 4-vinylguaiacol are originated from p-coumaric and ferulic acids. These phenolic compounds are valuable intermediates in the biotechnological production of new fragrances. In addition, gallic acid and its ester, methyl gallate, are metabolized to produce the powerful antioxidant pyrogallol. Therefore, it might be possible to use L. plantarum strains to obtain high-added-value antioxidants from the degradation of phenolic compounds found in wine wastes.

Plant cell factories as a source for anti-cancer lignans

2002 ◽  
Vol 1 (1) ◽  
pp. 27-35 ◽  
Author(s):  
R.R.J. Arroo ◽  
A.W. Alfermann ◽  
M. Medarde ◽  
M. Petersen ◽  
N. Pras ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Factories ◽  
Anti Cancer

Mucuna pruriens: Improvement of the biotechnological production of the anti-Parkinson drug L-dopa by plant cell selection

1993 ◽  
Vol 15 (6) ◽  
pp. 263-268 ◽  
Author(s):  
Niesko Pras ◽  
Herman J. Woerdenbag ◽  
Sieb Batterman ◽  
Jan F. Visser ◽  
Wim Uden
Keyword(s):  
Plant Cell ◽  
Mucuna Pruriens ◽  
Cell Selection ◽  
Biotechnological Production

scholarly journals Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

2021 ◽  
Author(s):  
Nora Lisa Bitzenhofer ◽  
Luzie Kruse ◽  
Stephan Thies ◽  
Benedikt Wynands ◽  
Thorsten Lechtenberg ◽  
...  
Keyword(s):  
Stress Response ◽  
Cell Envelope ◽  
Molecular Genetic ◽  
Membrane Integrity ◽  
Natural Compounds ◽  
Enzyme Engineering ◽  
Fine Tuning ◽  
Biotechnological Production ◽  
Cell Factories ◽  
Reactive Aldehydes

Abstract Biotechnological production in bacteria enables access to numerous valuable chemical compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well as the combinatorial use of biocatalysts, pathways, and circuits even bring new-to-nature compounds within reach. However, the associated substrates and biosynthetic products often cause severe chemical stress to the bacterial hosts. Species of the Pseudomonas clade thus represent especially valuable chassis as they are endowed with multiple stress response mechanisms, which allow them to cope with a variety of harmful chemicals. A built-in cell envelope stress response enables fast adaptations that sustain membrane integrity under adverse conditions. Further, effective export machineries can prevent intracellular accumulation of diverse harmful compounds. Finally, toxic chemicals such as reactive aldehydes can be eliminated by oxidation and stress-induced damage can be recovered. Exploiting and engineering these features will be essential to support an effective production of natural compounds and new chemicals. In this article, we therefore discuss major resistance strategies of Pseudomonads along with approaches pursued for their targeted exploitation and engineering in a biotechnological context. We further highlight strategies for the identification of yet unknown tolerance-associated genes and their utilisation for engineering next-generation chassis and finally discuss effective measures for pathway fine-tuning to establish stable cell factories for the effective production of natural compounds and novel biochemicals.

Biotechnological Production of Pharmaceuticals and Biopharmaceuticals in Plant Cell and Organ Cultures

2018 ◽  
Vol 25 (30) ◽  
pp. 3577-3596 ◽  
Author(s):  
Diego Hidalgo ◽  
Raul Sanchez ◽  
Liliana Lalaleo ◽  
Mercedes Bonfill ◽  
Purificacion Corchete ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Plant Cell ◽  
Organ Cultures ◽  
Biotechnological Production ◽  
Metabolic Genes ◽  
Plant Biofactories ◽  
Industrial Level ◽  
Specific Reactions ◽  
New Applications ◽  
Pharmaceutical Production

Background: Plant biofactories are biotechnological platforms based on plant cell and organ cultures used for the production of pharmaceuticals and biopharmaceuticals, although to date only a few of these systems have successfully been implemented at an industrial level. Metabolic engineering is possibly the most straightforward strategy to boost pharmaceutical production in plant biofactories, but social opposition to the use of GMOs means empirical approaches are still being used. <P><P> Plant secondary metabolism involves thousands of different enzymes, some of which catalyze specific reactions, giving one product from a particular substrate, whereas others can yield multiple products from the same substrate. This trait opens plant cell biofactories to new applications, in which the natural metabolic machinery of plants can be harnessed for the bioconversion of phytochemicals or even the production of new bioactive compounds. Synthetic biological pipelines involving the bioconversion of natural substrates into products with a high market value may be established by the heterologous expression of target metabolic genes in model plants. <P><P> Objective: To summarize the state of the art of plant biofactories and their applications for the pipeline production of cosme-, pharma- and biopharmaceuticals. <P><P> Results: In order to demonstrate the great potential of plant biofactories for multiple applications in the biotechnological production of pharmaceuticals and biopharmaceuticals, this review broadly covers the following: plant biofactories based on cell and hairy root cultures; secondary metabolite production; biotransformation reactions; metabolic engineering tools applied in plant biofactories; and biopharmaceutical production.

scholarly journals Advances and Prospects of Phenolic Acids Production, Biorefinery and Analysis

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 874 ◽  
Author(s):  
Egle Valanciene ◽  
Ilona Jonuskiene ◽  
Michail Syrpas ◽  
Ernesta Augustiniene ◽  
Paulius Matulis ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Large Scale ◽  
Microbial Cell ◽  
Free Radical Scavengers ◽  
Scale Production ◽  
Biotechnological Production ◽  
Natural Sources ◽  
Sustainable Technologies ◽  
Microbial Cell Factories ◽  
Cell Factories

Biotechnological production of phenolic acids is attracting increased interest due to their superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As secondary metabolites, primarily found in plants and fungi, they are effective free radical scavengers due to the phenolic group available in their structure. Therefore, phenolic acids are widely utilised by pharmaceutical, food, cosmetic, and chemical industries. A demand for phenolic acids is mostly satisfied by utilising chemically synthesised compounds, with only a low quantity obtained from natural sources. As an alternative to chemical synthesis, environmentally friendly bio-based technologies are necessary for development in large-scale production. One of the most promising sustainable technologies is the utilisation of microbial cell factories for biosynthesis of phenolic acids. In this paper, we perform a systematic comparison of the best known natural sources of phenolic acids. The advances and prospects in the development of microbial cell factories for biosynthesis of these bioactive compounds are discussed in more detail. A special consideration is given to the modern production methods and analytics of phenolic acids.

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