scholarly journals The Effect of Ethanol on Gelation, Nanoscopic, and Macroscopic Properties of Serum Albumin Hydrogels

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1927
Author(s):  
Seyed Hamidreza Arabi ◽  
David Haselberger ◽  
Dariush Hinderberger
Keyword(s):  
Drug Delivery ◽  
Serum Albumin ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
New Materials ◽  
Gel Properties ◽  
Paramagnetic Resonance ◽  
Physiological Conditions ◽  
Human Body Temperature ◽  
Electron Paramagnetic

Serum albumin has shown great potential in the development of new biomaterials for drug delivery systems. Different methods have been proposed to synthesis hydrogels out of serum albumin. It has been observed that ethanol can also act as a trigger for serum albumin denaturation and subsequent gelation. In this study, we focus on basic mechanisms of the albumin gelation process at 37 °C when using the chemical denaturant ethanol. The temperature of 37 °C was chosen to resemble human body temperature, and as under physiological conditions, albumin is in a non-denatured N conformation. As established in our previous publication for the triggers of pH and temperature (and time), we here explore the conformational and physical properties space of albumin hydrogels when they are ethanol-induced and show that the use of ethanol can be advisable for certain gel properties on the nanoscopic and macroscopic scale. To this end, we combine spectroscopic and mechanically (rheology) based data for characterizing the gels. We also study the gels′ binding capacities for fatty acids with electron paramagnetic resonance (EPR) spectroscopy, which implies observing the effects of bound stearic acids on gelation. Ethanol reduces the fraction of the strongly bound FAs in bovine serum albumin (BSA) hydrogels up to 52% and induces BSA hydrogels with a maximum storage modulus of 5000 Pa. The loosely bound FAs in ethanol-based hydrogels, besides their relatively weak mechanical properties, introduce interesting new materials for fast drug delivery systems and beyond.

scholarly journals Bioactive Betulin and PEG Based Polyanhydrides for Use in Drug Delivery Systems

2021 ◽  
Vol 22 (3) ◽  
pp. 1090
Author(s):  
Daria Niewolik ◽  
Barbara Bednarczyk-Cwynar ◽  
Piotr Ruszkowski ◽  
Tomasz R. Sosnowski ◽  
Katarzyna Jaszcz
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Drug Delivery Systems ◽  
Morphological Characteristics ◽  
Delivery Systems ◽  
Poly Ethylene Glycol ◽  
Physiological Conditions ◽  
Aerodynamic Properties ◽  
Poly Ethylene ◽  
Derivatives Of

In the course of this study, a series of novel, biodegradable polyanhydrides based on betulin disuccinate and dicarboxylic derivatives of poly(ethylene glycol) were prepared by two-step polycondensation. These copolymers can be used as carriers in drug delivery systems, in the form of microspheres. Betulin and its derivatives exhibit a broad spectrum of biological activity, including cytotoxic activity, which makes them promising substances for use as therapeutic agents. Microspheres that were prepared from betulin based polyanhydrides show promising properties for use in application in drug delivery systems, including inhalation systems. The obtained copolymers release the active substance—betulin disuccinate—as a result of hydrolysis under physiological conditions. The use of a poly(ethylene glycol) derivative as a co-monomer increases the solubility and bioavailability of the obtained compounds. Microspheres with diameters in the range of 0.5–25 µm were prepared by emulsion solvent evaporation method and their physicochemical and aerodynamic properties were analyzed. The morphological characteristics of the microspheres depended on the presence of poly(ethylene glycol) (PEG) segment within the structure of polyanhydrides. The porosity of the particles depended on the amount and molecular weight of the PEG used and also on the speed of homogenization. The most porous particles were obtained from polyanhydrides containing 20% wt. of PEG 600 by using a homogenization speed of 18,000 rpm.

scholarly journals Biodegradable Hybrid Block Copolymer – Lipid Vesicles as Potential Drug Delivery Systems

2019 ◽  
Author(s):  
Sanobar Khan ◽  
James McCabe ◽  
Kathryn Hill ◽  
Paul Beales
Keyword(s):  
Drug Delivery ◽  
Block Copolymer ◽  
Drug Delivery Systems ◽  
Release Kinetics ◽  
Lipid Vesicles ◽  
Delivery Systems ◽  
Burst Release ◽  
New Materials ◽  
Candidate Drug ◽  
Polymer Block

<p>The anticipated benefits of nano-formulations for drug delivery are well known: for nanomedicines to achieve this potential, new materials are required with predictive and tuneable properties. Excretion of excipients following delivery is advantageous to minimise the possibility of adverse effects; biodegradability to non-toxic products is therefore desirable. With this in mind, we aim to develop tuneable hybrid lipid-block copolymer vesicle formulations where the hydrophilic polymer block is polyethylene glycol (PEG), which has accepted biocompatibility, and the hydrophobic block of the polymer is biodegradable: polycaprolactone (PCL) or polylactide (PLA). We investigate five different block copolymers for the formation of 1:1 phospholipid:polymer hybrid vesicles, compare their properties to the appropriate unitary liposome (POPC) and polymersome systems and assess their potential for future development as nanomedicine formulations. The PEG-PCL polymers under investigation do not form polymersomes and exhibit poor colloidal and/or encapsulation stability in hybrid formulations with lipids. The properties of PEG-PLA hybrid vesicles are found to be more encouraging: they have much enhanced passive loading of a hydrophilic small molecule (carboxyfluorescein) compared to their respective polymersomes and exhibit more favourable release kinetics in the presence of serum compared to the liposome. Significantly, burst release from hybrid vesicles can be substantially reduced by making the polymer components of the hybrid vesicle a mixture containing 10 mol% of PEG<sub>16</sub>-PLA<sub>25</sub> that is intermediate in size between the phospholipid and larger PEG<sub>45</sub>-PLA<sub>54</sub> components. We conclude that hybrid lipid/PEG-PLA vesicles warrant further assessment and development as candidate drug delivery systems.</p>

scholarly journals Biodegradable Hybrid Block Copolymer – Lipid Vesicles as Potential Drug Delivery Systems

2019 ◽  
Author(s):  
Sanobar Khan ◽  
James McCabe ◽  
Kathryn Hill ◽  
Paul Beales
Keyword(s):  
Drug Delivery ◽  
Block Copolymer ◽  
Drug Delivery Systems ◽  
Release Kinetics ◽  
Lipid Vesicles ◽  
Delivery Systems ◽  
Burst Release ◽  
New Materials ◽  
Candidate Drug ◽  
Polymer Block

<p>The anticipated benefits of nano-formulations for drug delivery are well known: for nanomedicines to achieve this potential, new materials are required with predictive and tuneable properties. Excretion of excipients following delivery is advantageous to minimise the possibility of adverse effects; biodegradability to non-toxic products is therefore desirable. With this in mind, we aim to develop tuneable hybrid lipid-block copolymer vesicle formulations where the hydrophilic polymer block is polyethylene glycol (PEG), which has accepted biocompatibility, and the hydrophobic block of the polymer is biodegradable: polycaprolactone (PCL) or polylactide (PLA). We investigate five different block copolymers for the formation of 1:1 phospholipid:polymer hybrid vesicles, compare their properties to the appropriate unitary liposome (POPC) and polymersome systems and assess their potential for future development as nanomedicine formulations. The PEG-PCL polymers under investigation do not form polymersomes and exhibit poor colloidal and/or encapsulation stability in hybrid formulations with lipids. The properties of PEG-PLA hybrid vesicles are found to be more encouraging: they have much enhanced passive loading of a hydrophilic small molecule (carboxyfluorescein) compared to their respective polymersomes and exhibit more favourable release kinetics in the presence of serum compared to the liposome. Significantly, burst release from hybrid vesicles can be substantially reduced by making the polymer components of the hybrid vesicle a mixture containing 10 mol% of PEG<sub>16</sub>-PLA<sub>25</sub> that is intermediate in size between the phospholipid and larger PEG<sub>45</sub>-PLA<sub>54</sub> components. We conclude that hybrid lipid/PEG-PLA vesicles warrant further assessment and development as candidate drug delivery systems.</p>

scholarly journals A comprehensive review on polymeric micelles: a promising drug delivery carrier

2021 ◽  
Vol 10 (3) ◽  
pp. 102-107
Author(s):  
Ajay Kumar
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Drug Delivery Systems ◽  
Polymeric Micelles ◽  
Delivery Systems ◽  
Hydrophobic Drug ◽  
Drug Release Profile ◽  
Physiological Conditions ◽  
Drug Molecules ◽  
Drug Delivery Carrier

The main aim of drug delivery systems is to regulate the rate of drug release as per the patient's physiological conditions as well as the progression of the illness or as per the circadian rhythms. To achieve such objectives, the new drug delivery systems have been developed to provide the drug release profile, which is based on each patient's needs. Different researches have been done to create drug delivery carriers, focusing on targeting and delivering hydrophobic drug molecules. This review focuses on Polymeric Micelles as the promising drug delivery carrier due to its high stability, protective property against the harsh gastrointestinal environment.

scholarly journals Template-assisted extrusion of biopolymer nanofibers under physiological conditions

2016 ◽  
Vol 8 (10) ◽  
pp. 1059-1066 ◽  
Author(s):  
Mohammad Raoufi ◽  
Neda Aslankoohi ◽  
Christine Mollenhauer ◽  
Heike Boehm ◽  
Joachim P. Spatz ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Biomedical Applications ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Physiological Conditions

Biomedical applications ranging from tissue engineering to drug delivery systems require versatile biomaterials based on the scalable and tunable production of biopolymer nanofibers under physiological conditions.

Bovine serum albumin-sodium alkyl sulfates bioconjugates as drug delivery systems

2015 ◽  
Vol 130 ◽  
pp. 126-132 ◽  
Author(s):  
M. Benkő ◽  
N. Varga ◽  
D. Sebők ◽  
G. Bohus ◽  
Á. Juhász ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Sodium Alkyl Sulfates ◽  
Alkyl Sulfates

Techniques For The Preparation of Tissue Samples Containing Injectable Polymer Microcapsules

1985 ◽  
Vol 43 ◽  
pp. 710-711
Author(s):  
G.E. Visscher ◽  
R. L. Robison ◽  
G. J. Argentieri
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Gastrocnemius Muscle ◽  
Degradation Process ◽  
Delivery Systems ◽  
Tissue Reaction ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Injectable Polymer ◽  
Microscopic Techniques

The use of various bioerodable polymers as drug delivery systems has gained considerable interest in recent years. Among some of the shapes used as delivery systems are films, rods and microcapsules. The work presented here will deal with the techniques we have utilized for the analysis of the tissue reaction to and actual biodegradation of injectable microcapsules. This work has utilized light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques. The design of our studies has utilized methodology that would; 1. best characterize the actual degradation process without artifacts introduced by fixation procedures and 2. allow for reproducible results.In our studies, the gastrocnemius muscle of the rat was chosen as the injection site. Prior to the injection of microcapsules the skin above the sites was shaved and tattooed for later recognition and recovery. 1.0 cc syringes were loaded with the desired quantity of microcapsules and the vehicle (0.5% hydroxypropylmethycellulose) drawn up. The syringes were agitated to suspend the microcapsules in the injection vehicle.

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