Temperature-sensitive hydrogel loaded with DNase I alleviates epidural fibrosis in a mouse model of laminectomy

Excessive epidural fibrosis attached to the dura mater is the major cause of recurrent failed back surgery syndrome after spine surgery. Neutrophil extracellular traps (NETs) promote epidural fibrosis, raising the possibility that the DNA backbone of NETs may be a potential target in the therapy of epidural fibrosis. Human body temperature-sensitive hydroxypropyl chitin hydrogel solutions were prepared to encapsulate DNase I, which gradually decomposed in vivo. DNase I, which was released from temperature-sensitive hydrogels, destroyed the DNA backbone of NETs and dispersed the clustering of myeloperoxidase (MPO) in NETs. Evidence from MRI, H&E and Masson staining supported that hydroxypropyl chitin hydrogels loaded with DNase I were nontoxic and reduced epidural fibrosis. As expected, fibronectin in the wound was significantly abridged in the mice treated with hydrogels loaded with DNase I. Compared with the gelatin sponge absorbing DNase I, temperature-sensitive hydroxypropyl chitin hydrogels loaded with DNase I were more powerful in the therapy of epidural fibrosis. These results indicate that temperature-sensitive hydroxypropyl chitin hydrogels were effective in DNase I encapsulation and alleviation of epidural fibrosis in a mouse model of laminectomy.

Methodology of asystolic donor heart’s condition study in an experiment using small laboratory animals

In transplantation, there has always been an acute problem of the discrepancy between the number of donor organs and the number of recipients, including donor hearts. There are various ways to increase the pool of donor organs, one of them is the use of asystolic or non-heart-beating donors. Due to poor myocardial tolerance of ischemia during the asystole period, as well as because of the difficulties in diagnosing cardiac diseases of the asystolic donor, which can be contraindication to transplantation. Therefore, an in-depth study of the state of the myocardium in asystolic donors is required. Currently, there is no generally accepted protocol for working with asystolic heart donors. This protocol should include methods of heart conditioning and assessing of myocardium state. For its development we need more experimental and preclinical studies. A protocol for such a study is proposed. The modeling of an asystolic donor using rats is described on the basis of experimental work carried out by a team of authors. The article describes the following technical aspects: anesthetic guidance, asystole detection criterion, maintaining the rat body temperature in accordance with the human body temperature during cardiac arrest, surgical aspects of performing the main experimental model. The Langendorff model of isolated cardiac perfusion was chosen as the main model for assessing the state of the myocardium of a small laboratory animal. Intra-left ventricular pressure, volume of coronary blood flow, heart rate and the presence of post-reperfusion arrhythmias were selected as criteria for assessing the state of donor hearts. Assessment of the volume of damage to the donor heart is carried out using triphenyltetrazolium chloride staining of the donor organ.

Birefringent elastomers with Multiple Stimuli-Responses

Currently, even though birefringent soft materials have attracted considerable attention for many sensors and optical devices, it is still difficult for most of them to achieve multiple responses. Herein, we have designed a multiple-responding birefringent elastomer with high birefringence but low modulus by using 2-phenoxy-ethanoacrylate (PHEA) as monomer and 4-cyano-4'pentylbiphenyl as solvent. The excellent transparency (~90%) exhibited by the designed birefringent elastomer allowed us to observe the change of interference color. The obtained birefringent elastomer not only can be used as a photoelastic strain sensor with high sensitivity and fast response but also show a very sensitive response to heat, particularly in the range of the human body temperature. More interestingly, it shows excellent dielectric properties with a strong correlation between the interference color and the applied electric field, which allows easily writing and erasing the encrypted information. These unique multi-signal response features of our obtained birefringent elastomer shed light on the multiple information encryptions, the anti-counterfeiting, and the multifunctional sensors.

Denaturation of the SARS-CoV-2 spike protein under non-thermal microwave radiation

SARS-CoV-2, the virus that causes COVID-19, is still a widespread threat to society. The spike protein of this virus facilitates viral entry into the host cell. Here, the denaturation of the S1 subunit of this spike protein by 2.45 GHz electromagnetic radiation was studied quantitatively. The study only pertains to the pure electromagnetic effects by eliminating the bulk heating effect of the microwave radiation in an innovative setup that is capable of controlling the temperature of the sample at any desired intensity of the electromagnetic field. This study was performed at the internal human body temperature, 37 °C, for a relatively short amount of time under a high-power electromagnetic field. The results showed that irradiating the protein with a 700 W, 2.45 GHz electromagnetic field for 2 min can denature the protein to around 95%. In comparison, this is comparable to thermal denaturation at 75 °C for 40 min. Electromagnetic denaturation of the proteins of the virus may open doors to potential therapeutic or sanitation applications.

Pengukura Suhu dengan Ir MLX90614 dan NoDeMCU dan Membandingkan dengan Ds18B20 untuk pencegahan Covid 19

This research was carried out by comparing the DS18B20 sensor and the MLX 90614 sensor to detect the accuracy of detecting human body temperature which is used to detect Covid 19 symptoms. In this experiment, 10 trials were carried out with different human segments detected using the sensor. In the experiment, it was found that the MLX90614 sensor is more suitable to be used for development with an IoT-based system because it does not need to come into contact with the skin of the human body. The MLX90614 sensor will detect the temperature and change it to one Celsius unit then send it to the firebase database which will then be picked up by the android application which is held by the security officer so that the temperature can be known remotely. When the temperature is more than 38.5 Celsius it will turn on the buzzer sound which can be heard from a distance which indicates the temperature is above 38.5 Celsius and on android will also display a danger sign

Warm Compress on Lowering Body Temperature Among Hyperthermia Patients: A Literature Review

Hyperthermia increased the core human body temperature above normal 36.7-37.5 °C, usually caused by infection, resulting in fever, and was the most common manifestation. One of the efforts that could be done to overcome the symptoms of hyperthermia was the application of warm compresses to the frontal, axillary, and dorsalis pedis. The study aimed to describe body temperature changes in hyperthermic patients after warm compress was applied. This study used a descriptive design with a literature review approach. Twelve articles were included in this review by six journal databases: PubMed, JSTOR, Wiley Online Library, Sage Journal, Taylor and Francis Online, and Google Scholar. The selection was carried out by assessing articles that met the inclusion criteria, including the publication range for 2008-2021, English and Indonesian languages ??, and open access to full-text pdf. The critical assessment was carried out by using the Critical Appraisal Skills Programmed instrument. The review results showed that the warm compress method had a positive effect in lowering body temperature in the nursing process in patients with hyperthermia. Based on the literature from the reviewed articles, it could be concluded that a warm compress intervention needed to be given to hyperthermic patients to lower the patient's body temperature whether they were undergoing treatment or not

A Novel Smart Textiles To Reflect Emotion

The present study designs a wearable smart device regarding relationship between temperature and emotion. The device, amplifies, and sub-regionally transmits the current generated by the body temperature thermoelectric generator through a smart body temperature sensor. Different areas of clothing produce controllable and intelligent color, so that adult emotions can be understood through changes in clothing colors, which is conducive to judging their moods and promoting social interaction. Experimental results show that the device can accurately detect changes in human body temperature under hilarious, fearful, soothing, and angry emotions, so as to achieve changes in clothing colors, namely blue, red, green, and brown.

Study on a thermophysiological model for health assessment of showering environment in China

In China, the thermal environment of family showers in old communities is quite different from that of other living spaces, especially when the thermal environment changes drastically during showering, which can easily cause health problems. The human thermal physiological model is an effective tool to predict and evaluate the non-uniform and unstable shower thermal environment and human health risks. In this research, the showering experiment was carried out in a typical bathroom in an old community in China, during which environmental parameters such as air temperature, wall temperature and water temperature of the bathroom during the showering were recorded, and physiological parameters such as skin temperature, core temperature and blood pressure during the whole showering process were detected. Based on the multi-node numerical human body model of Stowljik and a cardiovascular control model with human body temperature as the driving force, a temperature-blood pressure coupling prediction model was established. The validity of the proposed model was examined for blood circulation. This predictive model can accurately reflect changes in physiological parameters and is verified as suitable for the health assessment of showering environment in residential buildings.

Biotribological performance of medical-grade UHMW polyethylene-based hybrid composite for joint replacement

Ultra high molecular weight polyethylene (UHMWPE) is widely used for articulating surfaces in total hip and knee replacements. In the present work, the tribological properties of UHMWPE-based nano composites were studied in order to meet the demands of current bearing applications. UHMWPE matrix reinforced with 0.5, 1, and 2 weight percentage of alumina nano powder were fabricated by hot pressing. The dispersion and microstructure of composite material was established by X-ray diffraction (XRD) and scanning electron microscope (SEM) micrograph. The tests were carried out on a reciprocating sliding pin-on-disc tribometer at human body temperature (37±1°C) under dry and human serum lubricating environments for a normal load of 46 N and 52 N, a constant sliding speed of 4 mm. Under these testing conditions, it has been observed that the wear behavior of the developed composites improved with increase in weight percentage of alumina nano powder. The results show that at 52 N load, the maximum value of wear rate was 7.9x10−7 mm3/Nm and the minimum value 1.6x10−7 mm3/Nm was obtained. SEM was used to examine the worn surface and it was observed that human serum adheres to the surface of the composite pins upon sliding, resulting in the formation of a film which results in better wear resistance of the composite pins under human serum lubrication than dry sliding. This study implies that the use of nano alumina power will reduce the wear of UHMWPE based composite under human serum lubrication.

The Influence of High Temperature Weather on Human Body Temperature Measurement by Infrared Thermal Imaging Thermometer

Infrared thermography thermometer is a non-contact temperature measuring equipment, which is widely used in the stage of large-scale epidemic of the covid-19 pandemic. It is used for rapid screening of human body temperature in crowded places at the entrance and exit of airports, docks, shopping malls, stations and schools. But when the outdoor temperature approaches or exceeds the body temperature in summer, can this method of measuring body surface temperature by infrared thermal imager be used as a standard for screening fever? Under the condition of high temperature in summer, the field experiment of measuring body temperature by infrared thermal imager is carried out, the experimental results are analyzed. We recommend the use of relative temperature difference for screening patients with fever.