scholarly journals Biological Activities of Aqueous Extracts from Carob Plant (Ceratonia siliqua L.) by Antioxidant, Analgesic and Proapoptotic Properties Evaluation

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3120
Author(s):  
Siwar Ben Ayache ◽  
Emna Behija Saafi ◽  
Fathi Emhemmed ◽  
Guido Flamini ◽  
Lotfi Achour ◽  
...  
Keyword(s):  
Biological Activities ◽  
Human Cell Line ◽  
Ceratonia Siliqua ◽  
Phytochemical Content ◽  
Methyl Hexanoate ◽  
Acid Methyl ◽  
Ceratonia Siliqua L ◽  
Carob Pod ◽  
Dose Dependent ◽  
Mcf 7

The present work describes the volatile compounds profile and phytochemical content of Ceratonia siliqua L. Fifty different components have been identified. Among them, three constituents are shared i.e., 2-methlybutanoic acid, methyl hexanoate and limonene by different common carob preparations: pulp decoction (PD), seeds decoction (SD) and Rob, a sweet syrup extracted from the pulp of the carob pod. Each extract exhibits different volatile aromatic emission profiles. The antioxidant activity of the extracts was evaluated using three methods, DPPH, ABTS and FRAP, producing a dose-dependent response. The IC50, when determined by FRAP, gave the lowest values (0.66 ± 0.01, 0.73 ± 0.05 and 0.55 ± 0.00 mg/mL PD, SD and Rob, respectively). The nociception essay, after intraperitoneal injection of acetic acid in mice, demonstrated that Rob, pulp and seeds decoction extracts showed an efficient inhibition of writhes over time, with persistence over 30 min. The SD decoction revealed the highest efficacy in decreasing the writhing reflex (90.3 ± 1.2%; p < 0.001). Furthermore, the proapoptotic activity of SD against three human cell line, THP-1, MCF-7 and LOVO, evaluated by flow cytometry, showed a significantly stronger proapoptotic activity on colon cancer (LOVO) than on the other cell lines, a phenomenon known as phenotypic selectivity.

Effects of aqueous extracts from Ceratonia siliqua L. pods on small intestinal motility in rats and jejunal permeability in mice

RSC Advances ◽  
2016 ◽  
Vol 6 (50) ◽  
pp. 44345-44353 ◽  
Author(s):  
Kaïs Rtibi ◽  
Slimen Selmi ◽  
Mohamed-Amine Jabri ◽  
Godefroy Mamadou ◽  
Nicolas Limas-Nzouzi ◽  
...  
Keyword(s):  
Intestinal Epithelium ◽  
Intestinal Motility ◽  
Gastrointestinal Transit ◽  
Aqueous Extracts ◽  
Ceratonia Siliqua ◽  
Small Intestinal Motility ◽  
Small Intestinal ◽  
Ceratonia Siliqua L ◽  
Carob Pod

The current study was performed to assess the effects of carob pod aqueous extracts (CPAE, pulp, seeds or mixture) on gastrointestinal transit (GIT) and intestinal epithelium permeability.

Supplementation with an insoluble fiber obtained from carob pod (Ceratonia siliqua L.) rich in polyphenols prevents dyslipidemia in rabbits through SIRT1/PGC-1α pathway

2017 ◽  
Vol 58 (1) ◽  
pp. 357-366 ◽  
Author(s):  
María Valero-Muñoz ◽  
Sandra Ballesteros ◽  
Baltasar Ruiz-Roso ◽  
Lourdes Pérez-Olleros ◽  
Beatriz Martín-Fernández ◽  
...  
Keyword(s):  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L ◽  
Carob Pod ◽  
Insoluble Fiber

Nutrient Content of Carob Pod (Ceratonia siliqua L.) Flour Prepared Commercially and Domestically

2009 ◽  
Vol 64 (4) ◽  
pp. 286-292 ◽  
Author(s):  
Faik A. Ayaz ◽  
Hülya Torun ◽  
Robert H. Glew ◽  
Zehra D. Bak ◽  
Luther T. Chuang ◽  
...  
Keyword(s):  
Nutrient Content ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L ◽  
Carob Pod

Antitumoral and Anticholinesterasic Activities of the Seven Species from Rubiaceae

2019 ◽  
Vol 20 (4) ◽  
pp. 302-308 ◽  
Author(s):  
Carla R.F. Volobuff ◽  
Pedro C.O. Junior ◽  
Sidney M. dos Santos ◽  
Zefa V. Pereira ◽  
Diego C. Ferreira ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Biological Activities ◽  
Brain Structures ◽  
Acetylcholinesterase Inhibition ◽  
Mato Grosso ◽  
Male Wistar Rats ◽  
First Time ◽  
Mato Grosso Do Sul ◽  
Antiproliferative Activities ◽  
Mcf 7

Background: The genus Psychotria and Palicourea are reported as a source of alkaloids and iridoids, which exhibit biological activities. This study aimed to evaluate antiproliferative and anticholinesterase activities and quantification of the alkaloids of seven species among the genus found in Mato Grosso do Sul region in Brazil. Methods: Concentrations of alkaloids were measured spectrophotometrically. The extracts were submitted to antiproliferative activity against ten cell lines. The anticholinesterase activity of the extracts was developed using brain structures of male Wistar rats: cerebral cortex, hippocampus, hypothalamus and striatum by the Ellman method. Results: Alkaloids from Psychotria and Palicourea species were quantified which showed values of 47.6 to 21.9 µg/g. Regarding the antiproliferative potential, Palicourea crocea demonstrated selectivity against the 786-0 cell line (GI50: 22.87 µg/mL). Psychotria leiocarpa inhibited cell growth against OVCAR-3 (GI50: 3.28 µg/mL), K-562 (GI50: 5.26 µg/mL), HaCaT (GI50: 27.20 µg/mL), PC-3 (GI50: 34.92 µg/mL), MCF-7 (GI50: 35.80 µg/mL) and P. capillacea showed activity against OVCAR-3 (GI50: 2.33 µg/ml) and U251 (GI50: 16.66 µg/ml). The effect of acetylcholinesterase inhibition was more effective in the hippocampus, demonstrating inhibition for Paliourea crocea, Psychotria deflexa, P. brachybotrya and P. leiocarpa of 70%, 57%, 50% and 40%, respectively, followed by P. poeppigiana and P. capillacea, inhibiting 21%, compared to the control. Conclusion: Herein, the present work showed for the first time, anticholinesterasic and antiproliferative activities of extracts of Palicourea and Psychotria seem to be mainly associated with the levels of alkaloids in the leaves of these species.

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