Quali–Quantitative Characterization of Volatile and Non-Volatile Compounds in Protium heptaphyllum (Aubl.) Marchand Resin by GC–MS Validated Method, GC–FID and HPLC–HRMS2

Protium heptaphyllum (Aubl.) Marchand (PH) trees are endemic to the tropical region of South America, mostly Brazil. Antibacterial, antinociceptive, anti-inflammatory, anxiolytic, antidepressant and anti-hyperlipidemic/anti-hypercholesterolemic effects were reported for its resinous exudate Protiumheptaphyllum resin (PHR). This work aims to provide a qualitative and quantitative consistent chemical profiling of the major constituents of this resin and two extracts enriched in acid (acidic triterpene concentrated extract, ATCE) and neutral triterpenes (α and β-amyrin concentrated extract, AMCE). GC–MS/GC–FID was used for volatile terpene fraction, a validated GC–MS method was developed for quantification of neutral α and β-amyrin and HPLC–APCI HRMS2 was used for acidic triterpenes analysis. The chemical investigation reported 29 molecules, including 14 volatile terpenes, 6 neutral triterpenes and 11 acid triterpenes. The most abundant compounds were α-amyrin (251.28 g kg−1, 123.98 g kg−1 and 556.82 g kg−1 in PHR, ATCE and AMCE, respectively), β-amyrin (172.66 g kg−1, 95.39 g kg−1 and 385.58 g kg−1 in PHR, ATCE and AMCE, respectively), 3-oxo-tirucalla-7,24-dien-21-oic acid (80.64 g kg−1, 157.10 g kg−1 and 15.31 g kg−1 in PHR, ATCE and AMCE, respectively) and 3α-hydroxy-tirucalla-8,24-dien-21-oic acid (77.71 g kg−1, 130.40 g kg−1 and 11.64 g kg−1 in PHR, ATCE and AMCE, respectively). Results showed specific enrichment of acidic and neutral triterpenoids in the two respective extracts.

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Graphene, an Interesting Nanocarbon Allotrope for Biosensing Applications: Advances, Insights, and Prospects

Biomedical Engineering and Computational Biology ◽

10.1177/1179597220983821 ◽

2021 ◽

Vol 12 ◽

pp. 117959722098382

Author(s):

Farid Menaa ◽

Yazdian Fatemeh ◽

Sandeep K Vashist ◽

Haroon Iqbal ◽

Olga N Sharts ◽

...

Keyword(s):

Cost Effective ◽

Genetic Alterations ◽

Multiplex Detection ◽

Quantitative Characterization ◽

Disease Biomarkers ◽

Qualitative And Quantitative ◽

Sensing Systems ◽

Doped Graphene ◽

Bioanalytical Applications

Graphene, a relatively new two-dimensional (2D) nanomaterial, possesses unique structure (e.g. lighter, harder, and more flexible than steel) and tunable physicochemical (e.g. electronical, optical) properties with potentially wide eco-friendly and cost-effective usage in biosensing. Furthermore, graphene-related nanomaterials (e.g. graphene oxide, doped graphene, carbon nanotubes) have inculcated tremendous interest among scientists and industrials for the development of innovative biosensing platforms, such as arrays, sequencers and other nanooptical/biophotonic sensing systems (e.g. FET, FRET, CRET, GERS). Indeed, combinatorial functionalization approaches are constantly improving the overall properties of graphene, such as its sensitivity, stability, specificity, selectivity, and response for potential bioanalytical applications. These include real-time multiplex detection, tracking, qualitative, and quantitative characterization of molecules (i.e. analytes [H2O2, urea, nitrite, ATP or NADH]; ions [Hg2+, Pb2+, or Cu2+]; biomolecules (DNA, iRNA, peptides, proteins, vitamins or glucose; disease biomarkers such as genetic alterations in BRCA1, p53) and cells (cancer cells, stem cells, bacteria, or viruses). However, there is still a paucity of comparative reports that critically evaluate the relative toxicity of carbon nanoallotropes in humans. This manuscript comprehensively reviews the biosensing applications of graphene and its derivatives (i.e. GO and rGO). Prospects and challenges are also introduced.

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Qualitative and Quantitative Characterization of Composition Heterogeneity on the Surface of Spray Dried Amorphous Solid Dispersion Particles by an Advanced Surface Analysis Platform with High Surface Sensitivity and Superior Spatial Resolution

Molecular Pharmaceutics ◽

10.1021/acs.molpharmaceut.8b00122 ◽

2018 ◽

Vol 15 (5) ◽

pp. 2045-2053 ◽

Cited By ~ 11

Author(s):

Sonal V. Bhujbal ◽

Dmitry Y. Zemlyanov ◽

Alex Cavallaro ◽

Sharad Mangal ◽

Lynne S. Taylor ◽

...

Keyword(s):

Solid Dispersion ◽

High Surface ◽

Amorphous Solid ◽

Amorphous Solid Dispersion ◽

Quantitative Characterization ◽

Qualitative And Quantitative ◽

Surface Sensitivity ◽

Analysis Platform ◽

Spray Dried

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Electrochemical Dissolution of Goethite by Abrasive Stripping Voltammetry

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19950950 ◽

1995 ◽

Vol 60 (6) ◽

pp. 950-959 ◽

Cited By ~ 20

Author(s):

Tomáš Grygar ◽

Jan Šubrt ◽

Jaroslav Boháček

Keyword(s):

Solid Phase ◽

Reaction Pathway ◽

Stripping Voltammetry ◽

Electrode Reaction ◽

Reductive Dissolution ◽

Quantitative Characterization ◽

Acid Media ◽

Qualitative And Quantitative ◽

Spectroscopic Examination

Abrasive stripping voltammetry was applied to the investigation of the reductive dissolution of some iron(III) oxides and hydroxy-oxides, particularly goethite (α-FeOOH), in acid media. The electrode reaction directly involves the solid phase, and the reaction pathway depends on the phase composition and particle shape. This can be used for a qualitative and quantitative characterization of goethite. The results of a quantitative analysis of a mixture of goethite (α-FeOOH) and lepidocrocite (γ-FeOOH) are compared with those of IR and Moessbauer spectroscopic examination. The effects of the particle appearance (shape, crystal intergrowth) on the results of the voltammetric and chronoamperometric measurements are discussed.

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