scholarly journals Evaluation of Hepatoprotective Activity and Oxidative Stress Reduction of Rosmarinus officinalis L. Shoots Tincture in Rats with Experimentally Induced Hepatotoxicity

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1737 ◽  
Author(s):  
Irina Ielciu ◽  
Bogdan Sevastre ◽  
Neli-Kinga Olah ◽  
Andreea Turdean ◽  
Elisabeta Chișe ◽  
...  
Keyword(s):  
Hepatoprotective Activity ◽  
Rosmarinus Officinalis ◽  
Raw Material ◽  
Carnosic Acid ◽  
Medicinal Uses ◽  
Antioxidant Mechanism ◽  
Rosmarinus Officinalis L ◽  
Experimentally Induced

Rosmarinus officinalis L. is a widely known species for its medicinal uses, that is also used as raw material for the food and cosmetic industry. The aim of the present study was to offer a novel perspective on the medicinal product originating from this species and to test its hepatoprotective activity. The tested sample consisted in a tincture obtained from the fresh young shoots. Compounds that are evaluated for this activity are polyphenols and terpenoids, that are identified and quantified by HPLC–UV–MS and GC–MS. Antioxidant activity was assessed in vitro, using the DPPH, FRAP and SO assays. Hepatoprotective activity was tested in rats with experimentally-induced hepatotoxicity. In the chemical composition of the tincture, phenolic diterpenes (carnosic acid, carnosol, rosmanol, rosmadial) and rosmarinic acid were found to be the majority compounds, alongside with 1,8-cineole, camphene, linalool, borneol and terpineol among monoterpenes. In vitro, the tested tincture proved significant antioxidant capacity. Results of the in vivo experiment showed that hepatoprotective activity is based on an antioxidant mechanism. In this way, the present study offers a novel perspective on the medicinal uses of the species, proving significant amounts of polyphenols and terpenes in the composition of the fresh young shoots tincture, that has proved hepatoprotective activity through an antioxidant mechanism.

Antidiabetic Effects and Mechanisms of Rosemary (Rosmarinus officinalis L.) and its Phenolic Components

2020 ◽  
Vol 48 (06) ◽  
pp. 1353-1368
Author(s):  
Tian-Qi Bao ◽  
Yi Li ◽  
Cheng Qu ◽  
Zu-Guo Zheng ◽  
Hua Yang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Scientific Evidence ◽  
Rosmarinus Officinalis ◽  
Carnosic Acid ◽  
In Vivo Studies ◽  
Rosemary Extract ◽  
Phenolic Components ◽  
Phenolic Constituents

Diabetes mellitus is a chronic endocrine disease result from absolute or relative insulin secretion deficiency, insulin resistance, or both, and has become a major and growing public healthy menace worldwide. Currently, clinical antidiabetic drugs still have some limitations in efficacy and safety such as gastrointestinal side effects, hypoglycemia, or weight gain. Rosmarinus officinalis is an aromatic evergreen shrub used as a food additive and medicine, which has been extensively used to treat hyperglycemia, atherosclerosis, hypertension, and diabetic wounds. A great deal of pharmacological research showed that rosemary extract and its phenolic constituents, especially carnosic acid, rosmarinic acid, and carnosol, could significantly improve diabetes mellitus by regulating glucose metabolism, lipid metabolism, anti-inflammation, and anti-oxidation, exhibiting extremely high research value. Therefore, this review summarizes the pharmacological effects and underlying mechanisms of rosemary extract and its primary phenolic constituents on diabetes and relative complications both in vitro and in vivo studies from 2000 to 2020, to provide some scientific evidence and research ideas for its clinical application.

Rosmarinus officinalis L. Essential Oil Inhibits In Vivo and In Vitro Leukocyte Migration

2011 ◽  
Vol 14 (9) ◽  
pp. 944-946 ◽  
Author(s):  
Gessilda Alcantara Nogueira de Melo ◽  
Renata Grespan ◽  
Jefferson Pitelli Fonseca ◽  
Thiago Oliveira Farinha ◽  
Expedito Leite Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Rosmarinus Officinalis ◽  
Leukocyte Migration ◽  
Rosmarinus Officinalis L

scholarly journals Evaluación in vitro de las actividades antimicrobiana, antioxidante y protectora del ADN, de un extracto etanólico de romero (Rosmarinus officinalis L.)

2018 ◽  
Vol 38 (2) ◽  
pp. 1-9
Author(s):  
Maria Eugenia Cecchini ◽  
Dardo Andrés Roma ◽  
Lucía Pussetto ◽  
Delvis Iglesias ◽  
María Claudia Basualdo ◽  
...  
Keyword(s):  
Rosmarinus Officinalis ◽  
Rosmarinus Officinalis L

 El objetivo del trabajo fue evaluar in vitro algunos potenciales efectos terapéuticos del extracto etanólico de romero (Rosmarinus officinalis L.), incluyendo sus actividades inhibitoria sobre la formación de biopelícula, antimicrobiana, antioxidante y protectora del ADN. La evaluación de la actividad antimicrobiana se llevó a cabo mediante la técnica de difusión en pocillo y  determinaciones de concentración inhibitoria mínima (CIM) y concentración bactericida mínima (CBM), empleando dos bacterias gram (+) y tres gram (-). La determinación de la actividad inhibitoria sobre la formación de biopelícula se realizó con el microorganismo que presentó mayor sensibilidad a la actividad antimicrobiana del romero, empleando una técnica en placa de 96 pocillos. La cuantificación de las actividades antioxidante y antigenotóxica se llevó a cabo sobre leucocitos caprinos, expuestos al extracto y a H2O2, mediante cuantificación de lipoperoxidación y daño en el ADN (ensayo cometa), respectivamente. Los resultados obtenidos indican mayor sensibilidad de los microorganismos gram (+) al extracto de romero (CIM/CBM 312,5/1250 y 312,5/2500 μg/ml para S. aureus y S. epidermidis, respectivamente), en tanto no se halló sensibilidad en los gram (-). Se encontró actividad inhibitoria sobre la formación de biopelícula aun a concentraciones subinhibitorias para S. aureus en placa de 96 pocillos. Finalmente, se demostró que el extracto de romero presenta actividad protectora del ADN en células caprinas que podría estar vinculada con un efecto antioxidante. Resulta necesario continuar investigando la capacidad antimicrobiana del extracto de romero para evaluar la posibilidad de emplearlo in vivo para el tratamiento de infecciones crónicas relacionadas con la presencia de biopelícula.

Phytochemical analysis and biological activity of selected Medicinal and Aromatic plants (Drimia numidica, Smilax aspera L., Rosmarinus officinalis L., Cannabis sativa L., Crocus sativus L.) against diabetes mellitus and cancer

2021 ◽  
Author(s):  
Ελένη Κακούρη
Keyword(s):  
Cannabis Sativa ◽  
Rosmarinus Officinalis ◽  
Crocus Sativus ◽  
Phytochemical Analysis ◽  
Aromatic Plants ◽  
Medicinal And Aromatic Plants ◽  
Rosmarinus Officinalis L ◽  
Crocus Sativus L

Η χρήση των φαρμακευτικών και αρωματικών φυτών είναι σήμερα ευρέως διαδεδομένη λόγω των πολλαπλών ωφελειών τους στην προαγωγή της καλής υγείας. Τα οφέλη από τη χρήση τους έχουν επιβεβαιωθεί μέσα από in vitro και in vivo μελέτες, επομένως γίνεται αντιληπτό πως τα φαρμακευτικά και αρωματικά φυτά θα συνεχίζουν να διαδραματίζουν καθοριστικό ρόλο ως σημαντικοί παράγοντες με σκοπό την προάσπιση της υγείας.Στην παρούσα διδακτορική διατριβή μελετήθηκε η χημική σύσταση καθώς και η in vitro και in vivo βιοδραστικότητα εκχυλισμάτων προερχόμενων από τα φυτά Drimia numidica, Smilax aspera L, Rosmarinus officinalis L, Cannabis sativa L., Crocus sativus L., καθώς και της κανναβιδιόλης, της μη ψυχότροπης ουσίας που προέρχεται από το φυτό της Cannabis sativa. Το χημικό προφίλ των εκχυλισμάτων προσδιορίστηκε με τη μέθοδο της υγρής χρωματογραφίας συζευγμένης με φασματομετρία μάζας χρόνου πτήσης, ενώ η εκτίμηση της βιοδραστικότητας των εκχυλισμάτων ως προς την αντιοξειδωτική τους ικανότητα έγινε με τις δοκιμασίες DPPH και ABTS. Η εκτίμηση του ολικού φαινολικού περιεχομένου πραγματοποιήθηκε με τη μέθοδο Folin-Ciocalteu.Δεδομένου του πλούσιου χημικού προφίλ των εκχυλισμάτων σε δευτερογενείς μεταβολίτες και σε συνδυασμό με την ισχυρή ή ακόμη και μέτρια αντιοξειδωτική δράση τους, έγιναν in vitro πειράματα που αφορούσαν στη διερεύνηση της κυτταροτοξικής τους δράσης σε κακοήθεις όγκους εγκεφάλου. Σε αυτή τη σειρά των πειραμάτων εξετάστηκε και η κυτταροτοξική δράση της διμεθυλοκροκετίνης, ενός ημι-φυσικού παραγώγου προερχόμενου από τα στίγματα του Crocus sativus L. Εξετάστηκαν δυο κυτταρικές σειρές και συγκεκριμένα η σειρά ΤΕ671 (κύτταρα μυελοβλαστώματος) και η σειρά Α172 (κύτταρα γλοιοβλαστώματος). Η δράση των εκχυλισμάτων παρακολουθήθηκε σε τρεις χρονικές στιγμές, δηλαδή στις 24, 48 και 72h, χρησιμοποιώντας την τεχνική Alamar blue. Τα αποτελέσματα έδειξαν ότι το φυτικό υλικό που μελετήθηκε καθώς και οι καθαρές ουσίες, διμεθυλοκροκετίνη και κανναβιδιόλη, αναστέλλουν την ανάπτυξη και τον πολλαπλασιασμό των καρκινικών κυττάρων με μία δόσο- και χρόνο- εξαρτώμενη σχέση. Στα in vivo πειράματα, μελετήθηκε η δράση εκχυλίσματος κροκινών οι οποίες εκχυλίστηκαν από τα στίγματα του φυτού Crocus sativus L. Τα πειράματα πραγματοποιήθηκαν χρησιμοποιώντας ως πειραματικό μοντέλο το zebrafish, και εκτιμήθηκε εάν οι κροκίνες μπορούν να ρυθμίσουν τα επίπεδα γλυκόζης στο zebrafish. Παρατηρήθηκε σημαντική μείωση των επιπέδων της γλυκόζης στις προνύμφες όπου χορηγήθηκαν κροκίνες. Ως εκ τούτου διερευνήθηκε η έκφραση του γονιδίου pck1, το οποίο ρυθμίζει τη διαδικασία της γλυκονεογένεσης. Τα αποτελέσματα έδειξαν σημαντική αύξηση έκφρασης του γονιδίου σε σχέση με τα έμβρυα στα οποία δεν χορηγήθηκαν κροκίνες, γεγονός που υποδηλώνει ότι η μείωση της γλυκόζης που παρατηρήθηκε, δεν είναι αποτέλεσμα παρεμπόδισης της γλυκονεογένεσης. Στη συνέχεια εξετάστηκε εάν οι κροκίνες επηρεάζουν το σχηματισμό των β-κυττάρων σε προνύμφες zebrafish. Χρησιμοποιήθηκε η διαγονιδιακή σειρά Tg(ins:DsRed) και τα αποτελέσματα έδειξαν πως οι κροκίνες ενεργοποίησαν την παραγωγή ινσουλίνης, όπως παρατηρήθηκε από την αυξημένη ένταση φθορισμού των παγκρεατικών νησιδίων, το οποίο επιβεβαιώθηκε μελετώντας και την έκφραση του γονιδίου insa. Παρατηρήθηκε υπερέκφραση του συγκεκριμένου γονιδίου σε σχέση με τα έμβρυα στα οποία δεν είχαν χορηγηθεί κροκίνες, γεγονός που οδηγεί στο συμπέρασμα ότι οι κροκίνες συμμετέχουν στη διαδικασία ομοιόστασης της γλυκόζης.

scholarly journals AVALIAÇÃO DAS POSSÍVEIS INTERAÇÕES POR DOCKING MOLECULAR DOS FITOCONSTITUINTES DO ÓLEO ESSENCIAL DE ROSMARINUS OFFICINALIS L. EM ENZIMAS DE CANDIDA SPP

2020 ◽  
Vol 7 (Único) ◽  
pp. 1461-1479
Author(s):  
Pedro Thiago Ramalho de Figueiredo ◽  
Laísa Vilar Cordeiro ◽  
Thamara Rodrigues de Melo ◽  
Giulian César da Silva Sá ◽  
Aleson Pereira de Sousa
Keyword(s):  
Protein Data Bank ◽  
Data Bank ◽  
Rosmarinus Officinalis ◽  
Candida Spp ◽  
Molegro Virtual Docker ◽  
Rosmarinus Officinalis L

A grande biodiversidade de plantas medicinais encontradas no Brasil apresenta grande importância para a medicina popular, pois diversas espécies de plantas medicinais são utilizadas para o tratamento e cura de doenças. Dessa maneira, as plantas medicinais apresentam potencial na descoberta e desenvolvimento de novos fármacos. Para auxiliar nos estudos de desenvolvimentos de novos fármacos, as técnicas quimioinformáticas se tornaram uma aliada dos pesquisadores ao reduzir tempo e gastos durante este processo. Neste sentido, o óleo essencial de Rosmarinus officinalis L. (Lamiaceae) é conhecida popularmente como alecrim-de-jardim, alecrim-de-cheiro e apresenta importante atividade antifúngica sendo um grande fator para a descoberta de novas substâncias químicas com potencial antifúngico. Este trabalho tem como objetivo, analisar por docking molecular as interações de metabólitos secundários encontrados no óleo essencial de Rosmarinus officinalis em diferentes enzimas de Candida spp. Os metabólitos secundários do óleo essencial de Rosmarinus officinalis foram obtidos da literatura, as enzimas foram adquiridas do Protein Data Bank sob código 5TZ1 (14α-lanosterol-demetilase) e 1EQC (exo-beta-(1,3)-glucanase) com seus respectivos ligantes. O docking molecular foi realizado utilizando o software Molegro Virtual Docker. Após análise do docking molecular, as substâncias tiveram melhor afinidade pela enzima 14α- lanosterol-demetilase, destacando o clovenol que apresentou energia de ligação semelhante ao inibidor cocristalizado. Com esse estudo podemos mostrar a importância do docking para a identificar possíveis locais de atuação de metabólitos secundários e sugerir que as substâncias químicas do óleo essencial podem atuar a nível de membrana celular sendo necessários estudos in vivo e in vitro para confirmação.

Aplicabilidade dos óleos vegetais em tônicos capilar: uma revisão

2021 ◽  
Vol 10 (15) ◽  
pp. e124101522725
Author(s):  
Lorena Stefany Souza Silva ◽  
Jânio Sousa Santos
Keyword(s):  
Ricinus Communis ◽  
Rosmarinus Officinalis ◽  
Rosmarinus Officinalis L ◽  
Pilocarpus Microphyllus

O mercado cosmético tem investido no suprimento dos interesses do grupo populacional Wellness que busca um estilo de vida mais saudável e sustentável ao meio ambiente, levando a valorização dos ativos vegetais e sua incorporação aos produtos cosméticos e de higiene. Nesse sentido, o presente estudo tem como objetivo abordar a empregabilidade dos óleos de Pilocarpus microphyllus, Rícinus communis e Rosmarinus officinalis na composição de tônicos naturais para crescimento capilar. Para tal, realizou-se uma revisão bibliográfica qualitativa na qual adotou-se como critérios que incluam documentos de cunho cientifico que apresentou relação com a aplicabilidade de óleos vegetais em cosméticos para crescimento capilar, em especial as loções tônicas. Nesse contexto, os óleos vegetais destacam-se como importantes constituinte que agregam valor ao produto o qual é incorporado, promovendo ações terapêuticas menos abrasivas aos utentes, sustentabilidade e potencial econômico gerado a agricultura familiar. Os óleos vegetais de Pilocarpus microphyllus, Rícino communis e Rosmarinus officinalis L. demonstraram características em comum para o tratamento antimicrobiano, vasodilatador e desobstrutor dos folículos pilosos, podendo ser empregados para a incorporação em tônicos para crescimento capilar. Sugere-se novas pesquisas de cunho experimentais in vitro e in vivo acerca da incorporação dos óleos vegetais em formulações para crescimento capilar.

scholarly journals Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2529
Author(s):  
Haeyeop Kim ◽  
Woo Seok Yang ◽  
Khin Myo Htwe ◽  
Mi-Nam Lee ◽  
Young-Dong Kim ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Related Proteins ◽  
Pro Inflammatory Cytokines ◽  
Inflammatory Effect

Dipterocarpus tuberculatus Roxb. has been used traditionally as a remedy for many diseases, especially inflammation. Therefore, we analyzed and explored the mechanism of the anti-inflammatory effect of a Dipterocarpus tuberculatus Roxb. ethanol extract (Dt-EE). Dt-EE clearly and dose-dependently inhibited the expression of pro-inflammatory cytokines such as IL-6, TNF-α, and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 cells. Also, Dt-EE suppressed the activation of the MyD88/TRIF-mediated AP-1 pathway and the AP-1 pathway related proteins JNK2, MKK4/7, and TAK1, which occurred as a result of inhibiting the kinase activity of IRAK1 and IRAK4, the most upstream factors of the AP-1 pathway. Finally, Dt-EE displayed hepatoprotective activity in a mouse model of hepatitis induced with LPS/D-galactosamine (D-GalN) through decreasing the serum levels of alanine aminotransferase and suppressing the activation of JNK and IRAK1. Therefore, our results strongly suggest that Dt-EE could be a candidate anti-inflammatory herbal medicine with IRAK1/AP-1 inhibitory and hepatoprotective properties.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

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