scholarly journals The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5948
Author(s):  
A. Sesilja Aranko ◽  
Hideo Iwaï
Keyword(s):  
Protein Splicing ◽  
Small Peptides ◽  
Single Domain Antibody ◽  
System A ◽  
Domain Antibody ◽  
15N Labeling ◽  
Extremely Halophilic Archaeon ◽  
Inducible Protein ◽  
The Impact ◽  
Proteins And Peptides

An efficient self-cleavable purification tag could be a powerful tool for purifying recombinant proteins and peptides without additional proteolytic processes using specific proteases. Thus, the intein-mediated self-cleavage tag was developed and has been commercially available as the IMPACT™ system. However, uncontrolled cleavages of the purification tag by the inteins in the IMPACT™ system have been reported, thereby reducing final yields. Therefore, controlling the protein-splicing activity of inteins has become critical. Here we utilized conditional protein splicing by salt conditions. We developed the inducible intein-mediated self-cleaving tag (IIST) system based on salt-inducible protein splicing of the MCM2 intein from the extremely halophilic archaeon, Halorhabdus utahensis and applied it to small peptides. Moreover, we described a method for the amidation using the same IIST system and demonstrated 15N-labeling of the C-terminal amide group of a single domain antibody (VHH).

scholarly journals Study of nitrogen compounds in different atmospheric compartments

2020 ◽  
Author(s):  
Christelle Ghaffar ◽  
Maxence Brissy ◽  
Binta Dieme ◽  
Martin Lerembourg ◽  
Marcello Brigante ◽  
...  
Keyword(s):  
Solid Phase ◽  
Inorganic Ions ◽  
Cloud Droplet ◽  
High Volume ◽  
Biological Functions ◽  
Small Peptides ◽  
Spe Method ◽  
The Impact ◽  
Proteins And Peptides ◽  
Inhibitor Solution

<p>Nitrogen molecules are present in the atmosphere also as inorganic ions (ammonium, nitrate…) and organic (peptides and proteins). Nowadays, there is still poor knowledge concerning the sources and fate of proteins and peptides in the atmosphere. In a larger context aiming at exploring microbial communities’ functioning in the atmosphere, the goal of this study is to examine the atmospheric proteome (peptide and protein contents, sequence, size distribution, biological functions) in the different atmospheric compartment (aerosols, cloud and rain waters).</p><p>Aerosols, cloud water and rain samples were collected at remote places nearby Clermont – Ferrand city (France) and from the Mountain “Puy de Dôme” (1465 m asl). Cloud droplet impactors and high volume impingers were used for allowing immediate fixation of the samples, by collecting within a protease inhibitor solution. Samples were filtered (0.22 µm porosity) and the proteins from water were digested using trypsin. The peptides were purified by solid phase extraction (SPE) method, concentrated by lyophilisation and analysed by LC – HRMS and MS². Preliminary results indicate the recurrence of certain small peptides in particular. Some of these most frequently observed peptides were selected as models for photochemical experiments. Briefly, these small peptides were irradiated under solar simulated conditions and products were identified by IC – MS, to assess the impact of such processes on the chemical composition of the atmosphere.</p><p>The next step will consist in analysing our observations along with chemical and biological parameters such as biodiversity, transcriptomic profiles, in order to better understand the interaction between microorganisms and atmosphere processes.</p>

Technology and Hospital Information System: A Case of Implementation of Electronic Clinical Records in Hospital Maternity Irene Neto in Lubango, Angola

2019 ◽  
Vol 10 (11) ◽  
pp. 1131-1135
Author(s):  
Tomas Hambili Paulo Sanjuluca ◽  
◽  
Ricardo Correia ◽  
Anabela Antunes de Almeida ◽  
Ana Gloria Diaz Martinez ◽  
...  
Keyword(s):  
Maternity Hospital ◽  
Management Support ◽  
Retrospective Case ◽  
System A ◽  
Clinical Records ◽  
Retrospective Case Study ◽  
The Impact ◽  
And Child Health

Introduction: In order to have a good assessment of the quality of maternal and child health care, it is essential that there is up-to-date and reliable information. Objective: To evaluate the impact of the implementation of a computerized database of clinical processes in the admission, archive and medical statistics section, of Maternity hospital Irene Neto/Lubango-Angola. Methodology: A descriptive study with a quantitative and qualitative approach to carry out a retrospective case study deliveries and newborns, records from 2014 to 2017. Final considerations: The implementation of this project may contribute to the improvement of clinical management support management of the hospital as well as facilitating access to information for research and scientific production.

The Impact of Political Transition on Ghana’s Educational System. A Case Study of the 4th Republic

2018 ◽  
Author(s):  
Emmanuel Owusu-Kwarteng ◽  
Prince Opoku ◽  
Gershon Dagba ◽  
Mark Amankwa
Keyword(s):  
Educational System ◽  
Political Transition ◽  
System A ◽  
The Impact

Impact Load Buffering Method Based on Stress Wave Attenuation Principle

2019 ◽  
Vol 11 (02) ◽  
pp. 1950019 ◽  
Author(s):  
Lin Gan ◽  
He Zhang ◽  
Cheng Zhou ◽  
Lin Liu
Keyword(s):  
Stress Wave ◽  
Wave Attenuation ◽  
Impact Load ◽  
Dynamics Simulation ◽  
Scanning System ◽  
Isolation Method ◽  
Element Analysis ◽  
System A ◽  
High Emission ◽  
The Impact

Rotating scanning motor is the important component of synchronous scanning laser fuze. High emission overload environment in the conventional ammunition has a serious impact on the reliability of the motor. Based on the theory that the buffer pad can attenuate the impact stress wave, a new motor buffering Isolation Method is proposed. The dynamical model of the new buffering isolation structure is established by ANSYS infinite element analysis software to do the nonlinear impact dynamics simulation of rotating scanning motor. The effectiveness of Buffering Isolation using different materials is comparatively analyzed. Finally, the Macht hammer impact experiment is done, the results show that in the experience of the 70,000[Formula: see text]g impact acceleration, the new buffering Isolation method can reduce the impact load about 15 times, which can effectively alleviate the plastic deformation of rotational scanning motor and improve the reliability of synchronization scanning system. A new method and theoretical basis of anti-high overload research for Laser Fuze is presented.

Statistical Approaches to Structural Change in Regional Interindustry Models

1986 ◽  
Vol 18 (9) ◽  
pp. 1189-1207
Author(s):  
B Ó Huallacháin
Keyword(s):  
Structural Change ◽  
Structural Changes ◽  
Washington State ◽  
Multivariate Statistical Analyses ◽  
Input Output ◽  
Multivariate Statistical ◽  
State Data ◽  
System A ◽  
Statistical Approaches ◽  
The Impact

The conventional approach to assessing structural change in regional input – output tables is to measure the impact of coefficient change on the estimation of outputs and multipliers. The methods developed and tested in this paper focus exclusively on the coefficients. Univariate and multivariate statistical analyses can be used to identify and measure various types of changes ranging from coefficient instability to changes in interindustry relationships as a system. A distinction is made between structural changes in input relationships and those in output relationships. The methods are tested by using Washington State data for the years 1963 and 1967. The results are compared with previous analyses of change in these data.

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