scholarly journals Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6299
Author(s):  
Paulina Żeliszewska ◽  
Monika Wasilewska ◽  
Michał Cieśla ◽  
Zbigniew Adamczyk
Keyword(s):  
Particle Deposition ◽  
Specific Antigen ◽  
Streaming Potential ◽  
Practical Significance ◽  
Colloid Particle ◽  
Label Free ◽  
Polymer Particles ◽  
Deposition Kinetics ◽  
Kinetics Of ◽  
Fibrinogen Molecule

The deposition kinetics of polymer particles with fibrinogen molecule coronas at bare and poly-L-lysine (PLL) modified mica was studied using the microfluid impinging-jet cell. Basic physicochemical characteristics of fibrinogen and the particles were acquired using dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using streaming potential measurements. Subsequently, an efficient method for the preparation of the particles with coronas, characterized by a controlled fibrinogen coverage, was developed. This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting label-free immunoassays governed by the specific antigen/antibody interactions.

scholarly journals Nanoparticle and Bioparticle Deposition Kinetics: Quartz Microbalance Measurements

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 145
Author(s):  
Anna Bratek-Skicki ◽  
Marta Sadowska ◽  
Julia Maciejewska-Prończuk ◽  
Zbigniew Adamczyk
Keyword(s):  
Particle Deposition ◽  
Quartz Crystal ◽  
Separation And Purification ◽  
Flow Conditions ◽  
Deposition Kinetics ◽  
Foam Stabilization ◽  
Controlled Deposition ◽  
Proper Interpretation ◽  
Kinetics Of ◽  
Theoretical Results

Controlled deposition of nanoparticles and bioparticles is necessary for their separation and purification by chromatography, filtration, food emulsion and foam stabilization, etc. Compared to numerous experimental techniques used to quantify bioparticle deposition kinetics, the quartz crystal microbalance (QCM) method is advantageous because it enables real time measurements under different transport conditions with high precision. Because of its versatility and the deceptive simplicity of measurements, this technique is used in a plethora of investigations involving nanoparticles, macroions, proteins, viruses, bacteria and cells. However, in contrast to the robustness of the measurements, theoretical interpretations of QCM measurements for a particle-like load is complicated because the primary signals (the oscillation frequency and the band width shifts) depend on the force exerted on the sensor rather than on the particle mass. Therefore, it is postulated that a proper interpretation of the QCM data requires a reliable theoretical framework furnishing reference results for well-defined systems. Providing such results is a primary motivation of this work where the kinetics of particle deposition under diffusion and flow conditions is discussed. Expressions for calculating the deposition rates and the maximum coverage are presented. Theoretical results describing the QCM response to a heterogeneous load are discussed, which enables a quantitative interpretation of experimental data obtained for nanoparticles and bioparticles comprising viruses and protein molecules.

Particle Deposition Kinetics of Colloidal Suspensions in Microchannels at High Ionic Strength

Langmuir ◽  
2017 ◽  
Vol 33 (26) ◽  
pp. 6471-6480 ◽  
Author(s):  
Cesare M. Cejas ◽  
Fabrice Monti ◽  
Marine Truchet ◽  
Jean-Pierre Burnouf ◽  
Patrick Tabeling
Keyword(s):  
Ionic Strength ◽  
Particle Deposition ◽  
Colloidal Suspensions ◽  
High Ionic Strength ◽  
Deposition Kinetics ◽  
Kinetics Of

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen

The Analyst ◽  
2021 ◽  
Author(s):  
Ruirui Zhao ◽  
Lu Zhao ◽  
Haidi Feng ◽  
Xiaoliang Chen ◽  
Huilin Zhang ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sensing Platforms ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

Fluorescence sensing platforms based on HCR and G-quadruplex DNAzyme amplification strategies for the detection of prostate-specific antigen.

Label-free electrochemical detection of prostate-specific antigen based on nucleic acid aptamer

2015 ◽  
Vol 68 ◽  
pp. 49-54 ◽  
Author(s):  
M. Souada ◽  
B. Piro ◽  
S. Reisberg ◽  
G. Anquetin ◽  
V. Noël ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Prostate Specific Antigen ◽  
Electrochemical Detection ◽  
Specific Antigen ◽  
Label Free

Kinetics of Particle Deposition in the Radial Impinging-Jet Cell

2001 ◽  
Vol 242 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Zbigniew Adamczyk ◽  
Barbara Siwek ◽  
Piotr Warszyński ◽  
Elizeusz Musiał
Keyword(s):  
Particle Deposition ◽  
Impinging Jet ◽  
Kinetics Of

Supercritical Fluid Deposition of Ruthenium Thin Films: A Kinetic Study

2007 ◽  
Vol 992 ◽  
Author(s):  
Christos F. Karanikas ◽  
James J. Watkins
Keyword(s):  
Thin Films ◽  
Growth Rate ◽  
Deposition Temperature ◽  
Zero Order ◽  
Measurable Effect ◽  
Deposition Rates ◽  
Reaction Pressure ◽  
Deposition Kinetics ◽  
First Order ◽  
Kinetics Of

AbstractThe kinetics of the deposition of ruthenium thin films from the hydrogen assisted reduction of bis(2,2,6,6-tetramethyl-3,5-heptanedionato)(1,5-cyclooctadiene)ruthenium(II), [Ru(tmhd)2cod], in supercritical carbon dioxide was studied in order to develop a rate expression for the growth rate as well as to determine a mechanism for the process. The deposition temperature was varied from 240°C to 280°C and the apparent activation energy was 45.3 kJ/mol. Deposition rates up to 30 nm/min were attained. The deposition rate dependence on precursor concentrations between 0 and 0.2 wt. % was studied at 260°C with excess hydrogen and revealed first order deposition kinetics with respect to precursor at concentrations lower then 0.06 wt. % and zero order dependence at concentrations above 0.06 wt. %. The effect of reaction pressure on the growth rate was studied at a constant reaction temperature of 260°C and pressures between 159 bar to 200 bar and found to have no measurable effect on the growth rate.

scholarly journals Proteomics in Diagnosis of Prostate Cancer/ Протеомика Во Дијагноза На Простатниот Карцином

PRILOZI ◽  
2015 ◽  
Vol 36 (1) ◽  
pp. 5-36 ◽  
Author(s):  
Katarina Davalieva ◽  
Momir Polenakovic
Keyword(s):  
Prostate Cancer ◽  
Tumor Tissue ◽  
Biomarker Discovery ◽  
Molecular Level ◽  
Specific Antigen ◽  
Shotgun Proteomics ◽  
Comparative Proteomics ◽  
Label Free ◽  
The Past ◽  
Proteomics Research

Abstract Prostate cancer (PCa) is the second most frequently diagnosed malignancy in men worldwide. The introduction of prostate specific antigen (PSA) has greatly increased the number of men diagnosed with PCa but at the same time, as a result of the low specificity, led to overdiagnosis, resulting to unnecessary biopsies and high medical cost treatments. The primary goal in PCa research today is to find a biomarker or biomarker set for clear and effecttive diagnosis of PCa as well as for distinction between aggressive and indolent cancers. Different proteomic technologies such as 2-D PAGE, 2-D DIGE, MALDI MS profiling, shotgun proteomics with label-based (ICAT, iTRAQ) and label-free (SWATH) quantification, MudPIT, CE-MS have been applied to the study of PCa in the past 15 years. Various biological samples, including tumor tissue, serum, plasma, urine, seminal plasma, prostatic secretions and prostatic-derived exosomes were analyzed with the aim of identifying diagnostic and prognostic biomarkers and developing a deeper understanding of the disease at the molecular level. This review is focused on the overall analysis of expression proteomics studies in the PCa field investigating all types of human samples in the search for diagnostics biomarkers. Emphasis is given on proteomics platforms used in biomarker discovery and characterization, explored sources for PCa biomarkers, proposed candidate biomarkers by comparative proteomics studies and the possible future clinical application of those candidate biomarkers in PCa screening and diagnosis. In addition, we review the specificity of the putative markers and existing challenges in the proteomics research of PCa.

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