scholarly journals Method Development and Validation for Omega-3 Fatty Acids (DHA and EPA) in Fish Using Gas Chromatography with Flame Ionization Detection (GC-FID)

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6592
Author(s):  
Suryati Muhammad Alinafiah ◽  
Azrina Azlan ◽  
Amin Ismail ◽  
Nor-Khaizura Mahmud Ab Rashid
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Good Precision ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Epa And Dha ◽  
Fish Samples

Gas chromatography with flame ionization detection (GC-FID) has often been used to quantify fatty acids in fish. This study validated the common method for determining omega-3 fatty acids (DHA and EPA) in the raw and cookedwarm-water fish, selayang, using GC-FID for subsequent evaluation on EPA and DHA retention using the Weibull model. The EPA and DHA were separated using a high-polarity capillary GC HP-88 column (60 m length, 0.25 mm ID, 0.2 μm DF) with a total run time of 45.87 min. The method was validated in linearity, precision, accuracy, specificity and sensitivity based on ICH requirements. In addition, it was found that the method had a high recovery rate (>95%) and good precision (RSD ≤ 2%) with overall RSDs ranging below 0.001% for both omega-3 PUFA. In conclusion, this method identified and quantified fatty acids and omega-3 accurately and precisely and can be used effectively for routine FAME analysis in fish samples.

Determination of selected fatty acids in dried sweat spot using gas chromatography with flame ionization detection

2016 ◽  
Vol 39 (22) ◽  
pp. 4377-4383 ◽  
Author(s):  
Roman Kanďár ◽  
Petra Drábková ◽  
Lenka Andrlová ◽  
Adam Kostelník ◽  
Alexander Čegan
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization

scholarly journals KANDUNGAN LEMAK DAN KOMPOSISI ASAM-ASAM LEMAK PADA BAGIAN BADAN IKAN JULUNG-JULUNG (Hemiramphus brasiliensis)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Louisa M. F. Josephus ◽  
Julius Pontoh ◽  
Lidya I. Momuat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Palmitic Acid ◽  
Eicosatrienoic Acid ◽  
Fat Content ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Water Heating ◽  
Epa And Dha

ABSTRAK Telah dilakukan penelitian mengenai kandungan lemak dan komposisi asam-asam lemak pada bagian badan ikan julung-julung. Penentuan kadar lemak pada penelitian ini menggunakan metode ekstraksi pemanasan dengan air (wet rendering). Kadar lemak pada bagian badan ikan julung-julung yaitu 0,02%. Ekstrak lemak ikan yang diperoleh di transesterifikasi basa menggunakan metode derivatisasi kemudian diinjeksikan pada alat kromatografi gas. Hasil penelitian ini menyimpulkan bahwa asam-asam lemak utama yang ada pada bagian badan ikan julung-julung adalah asam palmitat dan DHA. Sedangkan untuk asam-asam lemak omega-3 yang diperoleh yaitu asam eikosatrienoat, EPA dan DHA, dengan total kandungan omega-3 pada bagian badan yaitu 27,0%. ABSTRACT Research has been conducted for the fat content and composition of fatty acids in the part of body of the julung-julung fish. Determination of fat content in this study using the method of water heating extraction (wet rendering). Fat content in the part of body of the julung-julung fish is 0.02%. The extract of fish fat was transesterified bases using the derivatization method and then injected on a gas chromatography device. The results of this study prove that the main fatty acids present in the part of body of the julung-julung fish are palmitic acid and DHA. As for the omega-3 fatty acids obtained, namely eicosatrienoic acid, EPA and DHA, with the total omega-3 content in the part of body is 27.0%. 

Development of headspace–gas chromatography–flame ionization detection procedure to determine volatile fatty acids in zoo organic waste leachates

2011 ◽  
Vol 63 (12) ◽  
pp. 2873-2877 ◽  
Author(s):  
Anna Banel ◽  
Marta Wasielewska ◽  
Monika Felchner-Żwirełło ◽  
Bogdan Zygmunt
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Organic Waste ◽  
Volatile Fatty Acids ◽  
Sample Introduction ◽  
Flame Ionization Detection ◽  
Monocarboxylic Acids ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Headspace Gas

In order to determine volatile fatty acids (short chain monocarboxylic acids with 2 to 6 carbon atoms in a molecule) in leachates formed in organic waste piles a procedure based on static headspace (HS) coupled with gas chromatography (GC) have been proposed and optimized. The conditions of HS extraction and sample introduction into a gas chromatograph as well as of chromatographic separation were considered. Using flame ionization detection LODs were of the order of 0.02– 0.37 mg/L. The analyzed leachate collecting on and close to a zoo organic waste pile contained VFAs (Volatile Fatty Acids) at a concentration ranging from 5.5 mg/L to 0.88 g/L and from below LOD to 10.1 mg/L for pool water at some distance from the pile, respectively.

scholarly journals Evaluation of Major Fatty Acids Determination in Palm Oil by Gas Chromatography-Flame Ionization Detection

2016 ◽  
Vol 36 (03) ◽  
pp. 308 ◽  
Author(s):  
Moh Taufik ◽  
Hanifah Nuryani Lioe ◽  
Nancy Dewi Yuliana
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Palm Oil ◽  
Methyl Esters ◽  
Coefficient Of Determination ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Fatty Acids Analysis

The fatty acid composition of palm oil is the major factor influencing its physical and chemical properties. The purpose of this research was to evaluate the analytical performance of major fatty acids (palmitic acid, stearic acid, oleic acid, and linoleic acid) analysis in palm oil. Triglycerides of palm oil were derivatized to fatty acid methyl esters (FAMEs) by using boron trifluoride (BF3) in methanol. FAMEs were determined by gas chromatography-flame ionization detection (GC-FID) using DB-23 capillary column as stationary phase. The studied parameters were instrument performance analysis, the efficiency of fatty acid derivatization, stability of derivatized analytes, accuracy, repeatability, intra-lab reproducibility, ruggedness, and method uncertainty. The evaluation results showed the instrument linearity at a working range of 5 to 40 mg/mL marked by coefficient of determination (R2) between 0.991-0.995. Instrument limits of detection (LOD) and instrument limits of quantification (LOQ) for 4 major fatty acids analysis were 26-35 µg/mLand 86-128 µg/mL, respectively. The increase of fatty acid concentration led to the decrease of derivatization efficiency in the fatty acids analysis. The result also showed that derivatized analytes were stable during 24 h storage at freeze temperature. The average recovery values by spiking method with the spiking concentration at 50 and 90 mg/g sample were at 75-94 % for stearic and linoleic acids analysis, however those for palmitic and oleic acids analysis were considered very low (<40 %), due to their low derivatization efficiency. Repeatability and intra-lab reproducibility of 4 major fatty acids analysis were at acceptable ranges, 0.45-1.38 % and 1.15-2.03 %, respectively. Determination by varying the volume of derivatizing agent showed the rugged method. Uncertainty of repeatability (Ur) and uncertainty of reproducibility (Ur) were ranged at 1.84-9.02 mg/g and 1.40-10.65 mg/g, respectively. This method was considerably reliable for the analysis of less abundance fatty acids in palm oil, stearic and linoleic acids.

scholarly journals Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

2021 ◽  
Vol 65 (2) ◽  
pp. 149-157
Author(s):  
Anna Somogyi ◽  
Judit Mátyási ◽  
Zsófia Gór-Nagy ◽  
Farkas Sarnyai ◽  
Miklós Csala ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Trans Fatty Acids ◽  
Cultured Cells ◽  
Flame Ionization Detection ◽  
Linear Calibration ◽  
Physiological Concentration ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Free Process

Putative health effects of dietary trans fatty acids (TFAs) receive a growing attention; while very little is known about the metabolism of these special food components. In vitro studies carried out in cultured cells provide an efficient and standardizable approach to follow the metabolic fate of TFAs, but it requires suitable techniques for the quantitative measurement of FAs in cell samples. Here, the development and validation of a simple and reliable method for the quantification of a group of relevant FAs by gas chromatography and flame ionization detection is presented. Sample preparation used a fast one-step and chloroform-free process for simultaneous extraction and esterification, and chromatographic separation was achieved in 25 min using a Zebron ZB-88 capillary column. A linear calibration (of R2 >0.99) was obtained in the concentration range of 1-200 µg/mL for each FA. Recovery rate was 82 % for samples of non-esterified FAs and >95 % for complex lipids, such as ceramides, diglycerides and triglycerides. The LOD and LOQ were below 0.5 µg/mL, and a robust method precision was achieved (RSD % was below 6 % for each lipid classes). The present method was also tested on a cultured cell line with or without FA treatment at close to physiological concentration, and the observed changes in the metabolite concentration levels revealed characteristic differences between the metabolism of cis and trans unsaturated FAs.

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