scholarly journals KANDUNGAN LEMAK DAN KOMPOSISI ASAM-ASAM LEMAK PADA BAGIAN BADAN IKAN JULUNG-JULUNG (Hemiramphus brasiliensis)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Louisa M. F. Josephus ◽  
Julius Pontoh ◽  
Lidya I. Momuat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Palmitic Acid ◽  
Eicosatrienoic Acid ◽  
Fat Content ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Water Heating ◽  
Epa And Dha

ABSTRAK Telah dilakukan penelitian mengenai kandungan lemak dan komposisi asam-asam lemak pada bagian badan ikan julung-julung. Penentuan kadar lemak pada penelitian ini menggunakan metode ekstraksi pemanasan dengan air (wet rendering). Kadar lemak pada bagian badan ikan julung-julung yaitu 0,02%. Ekstrak lemak ikan yang diperoleh di transesterifikasi basa menggunakan metode derivatisasi kemudian diinjeksikan pada alat kromatografi gas. Hasil penelitian ini menyimpulkan bahwa asam-asam lemak utama yang ada pada bagian badan ikan julung-julung adalah asam palmitat dan DHA. Sedangkan untuk asam-asam lemak omega-3 yang diperoleh yaitu asam eikosatrienoat, EPA dan DHA, dengan total kandungan omega-3 pada bagian badan yaitu 27,0%. ABSTRACT Research has been conducted for the fat content and composition of fatty acids in the part of body of the julung-julung fish. Determination of fat content in this study using the method of water heating extraction (wet rendering). Fat content in the part of body of the julung-julung fish is 0.02%. The extract of fish fat was transesterified bases using the derivatization method and then injected on a gas chromatography device. The results of this study prove that the main fatty acids present in the part of body of the julung-julung fish are palmitic acid and DHA. As for the omega-3 fatty acids obtained, namely eicosatrienoic acid, EPA and DHA, with the total omega-3 content in the part of body is 27.0%. 

scholarly journals PENENTUAN KANDUNGAN LEMAK DAN KOMPOSISI ASAM-ASAM LEMAK PADA BAGIAN BADAN IKAN GABUS (Channa striata) BUDIDAYA DAN LIAR

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Alvy M. Umage ◽  
Julius Pontoh ◽  
Lidya I. Momuat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Extraction Method ◽  
Fat Content ◽  
The Body ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Channa Striata

ABSTRAK Telah dilakukan penentuan kandungan lemak dan komposisi asam-asam lemak pada bagian badan ikan gabus (Channa striata) budidaya dan liar. Penelitian ini bertujuan untuk mengetahui berapa kandungan lemak dan komposisi asam lemak pada bagian badan ikan gabus (Channa striata) budidaya dan liar. Kandungan lemak dilakukan menggunakan metode ektraksi dan diperoleh kandungan lemak bagian badan ikan gabus budidaya 0,07% dan ikan gabus liar 0,01%. Lemak ikan diderivatisasi dan dianalisis menggunakan kromatografi gas dan diperoleh komposisi asam lemak tertinggi pada bagian badan ikan gabus budidaya adalah asam lemak oleat 37,1% diikuti  dan diikuti oleh asam lemak palmitat 25,6% sedangkan asam lemak tertinggi pada bagian badan ikan gabus liar adalah asam lemak palmitat 28,6% dan diikuti asam lemak oleat 16,9%. Kandungan asam lemak omega-3 pada bagian badan ikan gabus budidaya 5,6% dan ikan gabus liar 9,0%. Kata kunci: Asam lemak, ikan gabus budidaya dan liar, kromatografi gas  ABSTRACT Determination of fat content and composition of fatty acids in the body of snakehead (Channa striata) from cultivated and wild sources. This study aims to determine how much fat content and composition fatty acids in the body of snakehead (Channa striata) from cultivated and wild sources. The fat content were carried out using the extraction method. The fat content of cultured snakehead body was 0.07% and that of wild fish was 0.01%. Fish fats were derivatized and analyzed using gas chromatography and the highest fatty acid composition on the body of cultivated snakehead fish is 37.1% oleic fatty acids followed by 25.6% palmitic fatty acids while the highest fatty acid in the body of a wild snakehead fish is 28.6% palmitic fatty acid and followed by 16.9% oleic fatty acid. The content of omega-3 fatty acids in the body of cultivated snakehead fish is 5.6% and 9.0% on wild snakehead fish. Keywords: Fatty acids, wild and cultivated snakehead fish, gas chromatography 

scholarly journals KANDUNGAN LEMAK DAN KOMPOSISI ASAM LEMAK OMEGA-3 PADA IKAN KAKAP MERAH (Aphareus furca)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Sofriani D. Bontjura ◽  
Julius Pontoh ◽  
Johnly A. Rorong
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Content ◽  
Eicosatrienoic Acid ◽  
Fat Content ◽  
The Body ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Omega 3 Fatty Acid ◽  
Positive Effects ◽  
Oil Extract

ABSTRAKIkan laut merupakan sumber makanan penghasil terbesar asam lemak omega-3. Senyawa ini telah banyak dibuktikan memberikan efek positif bagi kesehatan. Telah dilakukan penelitian untuk mengetahui  kadar lemak dan komposisi asam lemak omega-3 pada badan ikan kakap merah (Aphareus furca). Pengujian kadar lemak dilakukan menggunakan metode ekstraksi rendering. Ekstrak minyak ikan yang diperoleh ditransesterifikasi basa menggunakan metode derivatisasi dan diinjeksikan pada alat kromatografi gas. Bagian badan ikan kakap merah mengandung lemak sebesar 0,06%. Kandungan asam lemak omega-3 sebesar 26,8% yang terdiri dari asam linolenat 2,4%, eikosatrienoat 4,3%, eikosapentaenoat (EPA) 0,9% dan dokosaheksaenoat (DHA) 19,2%. ABSTRACTFishes are the biggest food source of omega-3 fatty acids. This compound has been proven to have many positive effects on health. Research has been conducted to determine the fat content and composition of omega-3 fatty acids in the body of red snapper (Aphareus furca). Fat content testing is done using the rendering extraction method. Fish oil extract that was obtained were transesterified base using the derivatization method and injected on a gas chromatography device. The red snapper's body contains 0.06% fat. The omega-3 fatty acid content is 26.8% consisting of 2.4% linolenic acid, 4.3% eicosatrienoic acid, 0.9% eicosapentaenoic acid (EPA) and 19.2% docosahexaenoic acid (DHA). 

scholarly journals Method Development and Validation for Omega-3 Fatty Acids (DHA and EPA) in Fish Using Gas Chromatography with Flame Ionization Detection (GC-FID)

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6592
Author(s):  
Suryati Muhammad Alinafiah ◽  
Azrina Azlan ◽  
Amin Ismail ◽  
Nor-Khaizura Mahmud Ab Rashid
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Good Precision ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Epa And Dha ◽  
Fish Samples

Gas chromatography with flame ionization detection (GC-FID) has often been used to quantify fatty acids in fish. This study validated the common method for determining omega-3 fatty acids (DHA and EPA) in the raw and cookedwarm-water fish, selayang, using GC-FID for subsequent evaluation on EPA and DHA retention using the Weibull model. The EPA and DHA were separated using a high-polarity capillary GC HP-88 column (60 m length, 0.25 mm ID, 0.2 μm DF) with a total run time of 45.87 min. The method was validated in linearity, precision, accuracy, specificity and sensitivity based on ICH requirements. In addition, it was found that the method had a high recovery rate (>95%) and good precision (RSD ≤ 2%) with overall RSDs ranging below 0.001% for both omega-3 PUFA. In conclusion, this method identified and quantified fatty acids and omega-3 accurately and precisely and can be used effectively for routine FAME analysis in fish samples.

scholarly journals Antioxidant, Antimicrobial Activities and Fatty Acid Components of Flower, Leaf, Stem and Seed of Hypericum scabrum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hexane Extracts ◽  
Hypericum Scabrum ◽  
Different Parts

The hexane extracts of flower, leaf, stem, and seed of Hypericum scabrum, which were collected from northwestern Iran, were obtained by extraction in a Soxhlet apparatus. The fatty acids were converted to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexane extract from the flower, leaf, stem, and seed contained 39.1%, 43.2%, 29.0%, and 37.6% of omega-3 fatty acids, respectively. The other main components of the flower extract were tetracosane (12.2%) and palmitic acid (9.3%), and that of the leaf extract was palmitic acid (7.4%). The stem and seed extracts contained bis(2-ethylhexyl)phthalate (18.7% and 35.7%), nonacosane (11.7% and 3.9%) and linoleic acid (6.5% and 6.9%) as major components. The hexane extracts of different parts from H. scabrum represent an important source of omega-3 fatty acids in several Hypericum species. The antioxidant activity of all hexane extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results indicate that hexane extracts from different parts of H. scabrum possess considerable antioxidant activity. The highest radical scavenging activity was detected in seed, which had an IC50 = 165 μg/mL. The antimicrobial activity of the extracts of those samples were determined against seven Gram-positive and Gram-negative bacteria ( Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae), as well as three fungi ( Candida albicans, Saccharomyces cerevisiae, and Aspergillus niger). The bioassay showed that the oil exhibited moderate antimicrobial activity. This study reveals that the all parts of this plant are attractive sources of fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

Comparative study of tissue deposition of omega-3 fatty acids from polar-lipid rich oil of the microalgae Nannochloropsis oculata with krill oil in rats

2015 ◽  
Vol 6 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Michael L. Kagan ◽  
Aharon Levy ◽  
Alicia Leikin-Frenkel
Keyword(s):  
Fatty Acids ◽  
Comparative Study ◽  
Polar Lipid ◽  
Rat Plasma ◽  
Nannochloropsis Oculata ◽  
Polar Lipids ◽  
Omega 3 Fatty Acids ◽  
Krill Oil ◽  
Omega 3 ◽  
Epa And Dha

An oil from micro-algae rich in EPA with no DHA and consisting of 15% polar lipids (phospholipids and glycolipids) showed equivalent uptake of EPA into rat plasma and organs as omega-3 krill oil consisting of EPA and DHA and 40% phospholipids.

scholarly journals Potential Benefits of Icosapent Ethyl on the Lipid Profile: Case Studies

2014 ◽  
Vol 8 ◽  
pp. CMC.S13571 ◽  
Author(s):  
Daniel E. Hilleman ◽  
Mark A. Malesker
Keyword(s):  
Fatty Acids ◽  
Case Reports ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Heart Association ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Icosapent Ethyl ◽  
Epa And Dha ◽  
Potential Benefits

The cardiovascular benefits of marine-derived omega-3 fatty acids are supported by epidemiologic and clinical studies. Both healthy patients and those with confirmed coronary heart disease are advised by the American Heart Association to consume omega-3 fatty acids either through dietary fatty fish or fish oil products. We present two case reports of patients with dyslipidemia who were switched from an omega-3 dietary supplement or a prescription omega-3 drug containing eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) to a new prescription EPA-only drug, icosapent ethyl (IPE). Products containing a combination of EPA and DHA, including dietary supplements and prescription products, are more likely to increase low-density lipoprotein cholesterol (LDL-C) levels compared with pure EPA-only products. The lipid profiles of these two patients were improved with IPE treatment, illustrating the potentially favorable effects of IPE compared with other products containing both EPA and DHA.

scholarly journals Omega-3 polyunsaturated fatty acids supplementation improve clinical symptoms in patients with covid-19: A randomized clinical trial

2021 ◽  
Author(s):  
Mohsen Sedighiyan ◽  
Hamed Abdollahi ◽  
Elmira Karimi ◽  
Mostafa Badeli ◽  
Reza Erfanian ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Liver Enzymes ◽  
Clinical Symptoms ◽  
Intervention Group ◽  
Clinical Signs ◽  
Control Group ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Body Pain

Objective: We hypothesized that omega-3 fatty acids would be an appropriate adjunct therapy for alleviating the inflammatory response and clinical manifestation in hospitalized patients with covid-19 disease. Methods: This was a single-blind randomized controlled trial in Amir-Alam hospital in Tehran. Thirty adult men and women diagnosed with covid-19 were allocated to either control group (receiving Hydroxychloroquine) or intervention group (receiving Hydroxychloroquine plus 2 grams of DHA+EPA) for 2 weeks. Primary outcome of the intervention including CRP, ESR as well as clinical symptoms including body pain, fatigue, appetite and olfactory and secondary outcomes including liver enzymes were determined at the baseline and after omega-3 supplementation. Clinical signs were measured using self-reported questionnaires. There were commercial kits for determination of CRP and liver enzymes concentrations in the serum of patients. For determination of ESR automated hematology analyzer was applied. Results: In comparison to control group, patients receiving omega-3 indicated favorable changes in all clinical symptoms except for olfactory ((p<0.001 for body pain and fatigue, p= 0.03 for appetite and p=0.21 for olfactory). Reducing effects of omega-3 supplementation compared to control group were also observed in the levels of ESR and CRP after treatment (p<0.001 for CRP and p=0.02 for ESR). However, no between group differences in the liver enzymes serum concentrations were observed after supplementation (p>0.05). Conclusion: Current observations are very promising and indicate that supplementation with moderate dosages of omega-3 fatty acids may be beneficial in the management of inflammation-mediated clinical symptoms in covid-19 patients. Key words: Covid-19, omega-3, inflammation, clinical symptoms

scholarly journals Effect of docosahexaenoic acid plus insulin on atherosclerotic human endothelial cells

2020 ◽  
Author(s):  
Aysan Eslami Abriz ◽  
Reza Rahbarghazi ◽  
Alireza Nourazarian ◽  
Çıgır Biray Avci ◽  
Soltan Ali Mahboob ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Endothelial Cells ◽  
Docosahexaenoic Acid ◽  
Palmitic Acid ◽  
Pcr Array ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Human Endothelial Cells ◽  
Array Analysis ◽  
Tnf Α

Abstract Background: Atherosclerosis is touted as one of the most critical consequences of diabetes mellitus indicated by local inflammation of endothelial cells. The Effect of Omega 3 fatty acids, mainly docosahexaenoic acid (DHA), has been investigated in cells after exposure to high doses of lipids. The current experiment aimed to address the modulatory effects of docosahexaenoic acid and insulin in palmitic-treated human endothelial cells. Methods: Human umbilical vein endothelial cells were treated with 1mM palmitic acid, 50μM insulin, 50μM docosahexaenoic acid, and their combination for 48 hours. Cell survival rate and apoptosis were measured using MTT and flow cytometry assays. The Griess assay detected NO levels. Protein levels of TNF-α, IL-6, and NF-κB were studied using ELISA and immunofluorescence imaging. The expression of genes participating in atherosclerosis was monitored using PCR array analysis. Results: Oil Red O staining showed the inhibitory effect of DHA and insulin to reduce the intracellular accumulation of palmitic acid. Both DHA and Insulin blunted palmitic acid detrimental effects on HUVECs indicated by an increased survival rate (p<0.05). The percent of apoptotic cells was decreased in palmitic-treated cells received insulin and DHA compared to palmitic-treated group (p<0.05). Based on our data, DHA and Insulin diminished the production of all inflammatory cytokines, TNF-α, IL-6, and NF-κB, in palmitic-treated cells (p<0.05). Similar to these data, NO production was also decreased in all groups treated with insulin and DHA compared to the palmitic-treated cells (p<0.05). PCR array analysis revealed the modulatory effect of DHA and insulin on the expression of atherosclerosis-related genes pre-treated with palmitic acid compared to the control group (p<0.05). Conclusion: DHA and Insulin could alter the dynamic growth and dysfunctional activity of human endothelial cells after treatment with palmitic acid. Taken together, Omega 3 fatty acids, along with insulin, could dictate specific cell behavior in endothelial cells in vitro

Sign in / Sign up

Export Citation Format

Share Document