scholarly journals Evaluation of Major Fatty Acids Determination in Palm Oil by Gas Chromatography-Flame Ionization Detection

2016 ◽  
Vol 36 (03) ◽  
pp. 308 ◽  
Author(s):  
Moh Taufik ◽  
Hanifah Nuryani Lioe ◽  
Nancy Dewi Yuliana
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Palm Oil ◽  
Methyl Esters ◽  
Coefficient Of Determination ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Fatty Acids Analysis

The fatty acid composition of palm oil is the major factor influencing its physical and chemical properties. The purpose of this research was to evaluate the analytical performance of major fatty acids (palmitic acid, stearic acid, oleic acid, and linoleic acid) analysis in palm oil. Triglycerides of palm oil were derivatized to fatty acid methyl esters (FAMEs) by using boron trifluoride (BF3) in methanol. FAMEs were determined by gas chromatography-flame ionization detection (GC-FID) using DB-23 capillary column as stationary phase. The studied parameters were instrument performance analysis, the efficiency of fatty acid derivatization, stability of derivatized analytes, accuracy, repeatability, intra-lab reproducibility, ruggedness, and method uncertainty. The evaluation results showed the instrument linearity at a working range of 5 to 40 mg/mL marked by coefficient of determination (R2) between 0.991-0.995. Instrument limits of detection (LOD) and instrument limits of quantification (LOQ) for 4 major fatty acids analysis were 26-35 µg/mLand 86-128 µg/mL, respectively. The increase of fatty acid concentration led to the decrease of derivatization efficiency in the fatty acids analysis. The result also showed that derivatized analytes were stable during 24 h storage at freeze temperature. The average recovery values by spiking method with the spiking concentration at 50 and 90 mg/g sample were at 75-94 % for stearic and linoleic acids analysis, however those for palmitic and oleic acids analysis were considered very low (<40 %), due to their low derivatization efficiency. Repeatability and intra-lab reproducibility of 4 major fatty acids analysis were at acceptable ranges, 0.45-1.38 % and 1.15-2.03 %, respectively. Determination by varying the volume of derivatizing agent showed the rugged method. Uncertainty of repeatability (Ur) and uncertainty of reproducibility (Ur) were ranged at 1.84-9.02 mg/g and 1.40-10.65 mg/g, respectively. This method was considerably reliable for the analysis of less abundance fatty acids in palm oil, stearic and linoleic acids.

Determination of selected fatty acids in dried sweat spot using gas chromatography with flame ionization detection

2016 ◽  
Vol 39 (22) ◽  
pp. 4377-4383 ◽  
Author(s):  
Roman Kanďár ◽  
Petra Drábková ◽  
Lenka Andrlová ◽  
Adam Kostelník ◽  
Alexander Čegan
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization

Development of headspace–gas chromatography–flame ionization detection procedure to determine volatile fatty acids in zoo organic waste leachates

2011 ◽  
Vol 63 (12) ◽  
pp. 2873-2877 ◽  
Author(s):  
Anna Banel ◽  
Marta Wasielewska ◽  
Monika Felchner-Żwirełło ◽  
Bogdan Zygmunt
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Organic Waste ◽  
Volatile Fatty Acids ◽  
Sample Introduction ◽  
Flame Ionization Detection ◽  
Monocarboxylic Acids ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Headspace Gas

In order to determine volatile fatty acids (short chain monocarboxylic acids with 2 to 6 carbon atoms in a molecule) in leachates formed in organic waste piles a procedure based on static headspace (HS) coupled with gas chromatography (GC) have been proposed and optimized. The conditions of HS extraction and sample introduction into a gas chromatograph as well as of chromatographic separation were considered. Using flame ionization detection LODs were of the order of 0.02– 0.37 mg/L. The analyzed leachate collecting on and close to a zoo organic waste pile contained VFAs (Volatile Fatty Acids) at a concentration ranging from 5.5 mg/L to 0.88 g/L and from below LOD to 10.1 mg/L for pool water at some distance from the pile, respectively.

scholarly journals FATTY ACIDS ANALYSIS AND CHEMOTAXONOMIC CONSIDERATIONS OF MALVOIDEAE (MALVACEAE) SPECIES

Química Nova ◽  
2020 ◽  
Author(s):  
Diégina Fernandes ◽  
Otemberg Chaves ◽  
Yanna Teles ◽  
Maria Agra ◽  
Maria Vieira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Euclidean Distance ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Principal Component ◽  
Fatty Acid Analysis ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Fatty Acids Analysis

Previous researches showed that fatty acids analysis might be a useful tool to support the taxonomic investigation. In this approach the fatty acids content of ten Malvoideae species was analyzed and its chemotaxonomic significance has been investigated. The aerial parts of the species were collected in the Northeast of Brazil and their fatty acid methyl esters were analyzed by gas chromatography with flame ionization detector. The chemometric analysis consisted of principal component analysis (PCA) and hierarchical clustering analysis (HCA) with the euclidean distance between the samples given by the Ward.D2 algorithm. This is the first report of fatty acids from Wissadula peripocifolia, Herissantia crispa, Bakeridesia pickelii, Sidastrum micranthum, Pavonia cancellata and Pavonia malacophylla. The results showed the predominance of palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids in the studied species. By the PCA and HCA analysis, the fatty acid composition was able to distinguish the species Herissantia crispa and Pavonia malacophylla. Our findings showed a chemotaxonomic proximity among species from different genera reflecting the taxonomic and phylogenetic closeness previously demonstrated by molecular investigations on Malvoidae species. Furthermore, our results demonstrated that the fatty acid analysis may be an interesting tool to support the taxonomic investigations on Malvoideae species.

scholarly journals DETERMINATION OF FATTY ACIDS IN SOLANUM SURATTENSE BURM. F. BY USING GAS CHROMATOGRAPHY

2017 ◽  
Vol 10 (8) ◽  
pp. 60 ◽  
Author(s):  
Raman Preet ◽  
Raghbir Chand Gupta
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Methyl Esters ◽  
Basic Research ◽  
Fatty Acid Analysis ◽  
Operating Conditions ◽  
Solanum Surattense ◽  
Flame Ionization

  Objective: This study aims to document the fatty acid composition of Solanum surattense Burm. f. collected from hot desert of India, Rajasthan.Methods: The fatty acid analysis was performed by gas chromatography-flame ionization detector (GC-FID). The operating conditions used to examine methyl esters of fatty acids are as follows. Fatty acids were converted into methyl esters (FAMEs) before GC analysis according to the standard methods by Ranganna (1986). Quantitative determinations of FAMEs were conducted using GC-FID and capillary column HP-88 Agilent Technologies.Results: The most abundant fatty was palmitic acid (13.2%), oleic acid (22.9%), and linoleic acid (11.9%). This plant is good source of important fatty acids including all the groups of saturated, monounsaturated, and polyunsaturated fatty acids (MUFAs and PUFAs) and can be used as a commercial source of fatty acids especially MUFAs and PUFAs.Conclusion: The plant is well studied for various pharmacological activities such as antiasthmatic, anticancer, cardiovascular, and hepatoprotective. Determination of fatty acid profiles in nutritional and clinical research with precision and fastness has become popular for human health and basic research.

scholarly journals Method Development and Validation for Omega-3 Fatty Acids (DHA and EPA) in Fish Using Gas Chromatography with Flame Ionization Detection (GC-FID)

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6592
Author(s):  
Suryati Muhammad Alinafiah ◽  
Azrina Azlan ◽  
Amin Ismail ◽  
Nor-Khaizura Mahmud Ab Rashid
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Flame Ionization Detection ◽  
Good Precision ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Epa And Dha ◽  
Fish Samples

Gas chromatography with flame ionization detection (GC-FID) has often been used to quantify fatty acids in fish. This study validated the common method for determining omega-3 fatty acids (DHA and EPA) in the raw and cookedwarm-water fish, selayang, using GC-FID for subsequent evaluation on EPA and DHA retention using the Weibull model. The EPA and DHA were separated using a high-polarity capillary GC HP-88 column (60 m length, 0.25 mm ID, 0.2 μm DF) with a total run time of 45.87 min. The method was validated in linearity, precision, accuracy, specificity and sensitivity based on ICH requirements. In addition, it was found that the method had a high recovery rate (>95%) and good precision (RSD ≤ 2%) with overall RSDs ranging below 0.001% for both omega-3 PUFA. In conclusion, this method identified and quantified fatty acids and omega-3 accurately and precisely and can be used effectively for routine FAME analysis in fish samples.

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