scholarly journals Label-Free Visualization and Tracking of Gold Nanoparticles in Vasculature Using Multiphoton Luminescence

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2239
Author(s):  
Sean Burkitt ◽  
Mana Mehraein ◽  
Ramunas K. Stanciauskas ◽  
Jos Campbell ◽  
Scott Fraser ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Metallic Nanoparticles ◽  
Multiphoton Microscopy ◽  
Regulatory Agencies ◽  
Label Free ◽  
Imaging Approach ◽  
Luminescent Signal ◽  
Real Time Identification

Gold nanoparticles continue to generate interest for use in several biomedical applications. Recently, researchers have been focusing on exploiting their dual diagnostic/therapeutic theranostic capabilities. Before clinical translation can occur, regulatory agencies will require a greater understanding of their biodistribution and safety profiles post administration. Previously, the real-time identification and tracking of gold nanoparticles in free-flowing vasculature had not been possible without extrinsic labels such as fluorophores. Here, we present a label-free imaging approach to examine gold nanoparticle (AuNP) activity within the vasculature by utilizing multiphoton intravital microscopy. This method employs a commercially available multiphoton microscopy system to visualize the intrinsic luminescent signal produced by a multiphoton absorption-induced luminescence effect observed in single gold nanoparticles at frame rates necessary for capturing real-time blood flow. This is the first demonstration of visualizing unlabeled gold nanoparticles in an unperturbed vascular environment with frame rates fast enough to achieve particle tracking. Nanoparticle blood concentration curves were also evaluated by the tracking of gold nanoparticle flow in vasculature and verified against known pre-injection concentrations. Half-lives of these gold nanoparticle injections ranged between 67 and 140 s. This label-free imaging approach could provide important structural and functional information in real time to aid in the development and effective analysis of new metallic nanoparticles for various clinical applications in an unperturbed environment, while providing further insight into their complex uptake and clearance pathways.

scholarly journals Multiphoton imaging reveals axial differences in metabolic autofluorescence signals along the kidney proximal tubule

2018 ◽  
Vol 315 (6) ◽  
pp. F1613-F1625 ◽  
Author(s):  
Milica Bugarski ◽  
Joana Raquel Martins ◽  
Dominik Haenni ◽  
Andrew M. Hall
Keyword(s):  
Mitochondrial Function ◽  
Redox State ◽  
Kidney Diseases ◽  
Multiphoton Microscopy ◽  
Kidney Tissue ◽  
Kidney Cortex ◽  
Label Free ◽  
Multiphoton Imaging ◽  
Imaging Approach ◽  
Lactate And Pyruvate

Kidney proximal tubules (PTs) are densely packed with mitochondria, and defects in mitochondrial function are implicated in many kidney diseases. However, little is known about intrinsic mitochondrial function within PT cells. Here, using intravital multiphoton microscopy and live slices of mouse kidney cortex, we show that autofluorescence signals provide important functional readouts of redox state and substrate metabolism and that there are striking axial differences in signals along the PT. Mitochondrial NAD(P)H intensity was similar in both PT segment (S)1 and S2 and was sensitive to changes in respiratory chain (RC) redox state, whereas cytosolic NAD(P)H intensity was significantly higher in S2. Mitochondrial NAD(P)H increased in response to lactate and butyrate but decreased in response to glutamine and glutamate. Cytosolic NAD(P)H was sensitive to lactate and pyruvate and decreased dramatically in S2 in response to inhibition of glucose metabolism. Mitochondrial flavoprotein (FP) intensity was markedly higher in S2 than in S1 but was insensitive to changes in RC redox state. Mitochondrial FP signal increased in response to palmitate but decreased in response to glutamine and glutamate. Fluorescence lifetime decays were similar in both S1 and S2, suggesting that intensity differences are explained by differences in abundance of the same molecular species. Expression levels of known fluorescent mitochondrial FPs were higher in S2 than S1. In summary, substantial metabolic information can be obtained in kidney tissue using a label-free live imaging approach, and our findings suggest that metabolism is tailored to the specialized functions of S1 and S2 PT segments.

Electrochemical Aptasensor for Label-Free Detection of Protein Based on Gold Nanoparticle Involved Self-Assembly

2013 ◽  
Vol 310 ◽  
pp. 177-182
Author(s):  
Song Bai Zhang ◽  
Bing Jun Zhang ◽  
Qian Liu ◽  
Xia Hu ◽  
Li Ying Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Gold Electrode ◽  
Hot Water ◽  
Target Protein ◽  
Capture Probe ◽  
Label Free ◽  
Label Free Detection ◽  
Self Assembled

A label-free electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self assembly for the detection of protein is proposed using platelet derived growth factor-BB dimer (PDGF-BB) as the model analyte. Utilizing the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The aptamer probe for target protein hybridizes with the capture probe and the biosensor is fabricated. By measuring ac current voltammetry, the target protein can be sensitively detected in a linear dynamic range from 1-1000 ng/mL with a low detection limit of 0.5 ng/mL. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

A gold nanoparticle-based label free colorimetric aptasensor for adenosine deaminase detection and inhibition assay

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1572-1577 ◽  
Author(s):  
Fen Cheng ◽  
Yue He ◽  
Xiao-Jing Xing ◽  
Dai-Di Tan ◽  
Yi Lin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Adenosine Deaminase ◽  
Gold Nanoparticle ◽  
Label Free ◽  
Inhibition Assay ◽  
Free Gold ◽  
Novel Strategy ◽  
Colorimetric Aptasensor

A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The strategy has been employed for the assay of adenosine deaminase (ADA) activity.

Analyte-triggered autocatalytic amplification combined with gold nanoparticle probes for colorimetric detection of heavy-metal ions

2017 ◽  
Vol 53 (54) ◽  
pp. 7477-7480 ◽  
Author(s):  
Juanhua Yang ◽  
Yun Zhang ◽  
Lang Zhang ◽  
Huili Wang ◽  
Jinfang Nie ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Gold Nanoparticles ◽  
Metal Ions ◽  
Gold Nanoparticle ◽  
Heavy Metal Ions ◽  
Colorimetric Detection ◽  
Detection Limits ◽  
Label Free ◽  
Nanoparticle Probes ◽  
Free Gold

This work reports a new nanosensor based on analyte-triggered autocatalytic amplification and label-free gold nanoparticles for the colorimetric detection of Hg2+, Cu2+and Ag+with detection limits less than 3 nM.

scholarly journals Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

2020 ◽  
Vol 2 (12) ◽  
pp. 5737-5745
Author(s):  
Tejaswini Appidi ◽  
Sushma V. Mudigunda ◽  
Suseela Kodandapani ◽  
Aravind Kumar Rengan
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Label Free ◽  
Free Gold ◽  
Clinical Point

“C-ColAur” technique for colorimetric detection of cervical cancer by in situ formation of gold nanoparticles.

Direct imaging of single gold nanoparticle etching: sensitive detection of lead ions

2014 ◽  
Vol 6 (13) ◽  
pp. 4507-4511 ◽  
Author(s):  
Dinggui Dai ◽  
Dong Xu ◽  
Xiaodong Cheng ◽  
Yan He
Keyword(s):  
Gold Nanoparticles ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Detection Method ◽  
Size Reduction ◽  
Direct Imaging ◽  
Real Time Imaging ◽  
Highly Sensitive ◽  
Darkfield Microscopy

We present a highly sensitive Pb2+ detection method by in situ real-time imaging of Pb2+ catalyzed etching and size reduction of immobilized single gold nanoparticles with darkfield microscopy.

Programmable assembly of gold nanoparticle nanoclusters and lattices

2020 ◽  
Vol 8 (31) ◽  
pp. 6810-6813
Author(s):  
Luyao Shen ◽  
Victor Pan ◽  
Haofei Li ◽  
Yunlong Zhang ◽  
Pengfei Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Metallic Nanoparticles

Deterministic assembly of metallic nanoparticles (e.g. gold nanoparticles) into prescribed configurations has promising applications in many fields such as biosensing and drug delivery.

scholarly journals Real-Time Multidepth Multiphoton Microscopy Using Pixel-to-Pixel Focus-Switching

2020 ◽  
Vol 10 (20) ◽  
pp. 7173
Author(s):  
Yifan Qin ◽  
Deying Chen ◽  
Yuanqin Xia
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Cost Effective ◽  
Axial Direction ◽  
Three Dimensions ◽  
Mouse Lung ◽  
Label Free

Multiphoton microscopy is a well-established technique for biomedical applications, but real-time multidepth multimodal multiphoton microscopy using non-imaging detection has barely been discussed. We demonstrate a novel label-free imaging system capable of generating multimodal multiphoton signals at different focal planes simultaneously. Two spatially overlapped and temporally interlaced beams are obtained by applying cost-effective electro-optic modulator (EOM)-based fast-switching light paths. The switching beams have different divergence properties, enabling imaging at different depths into samples. The EOM is synchronized to the pixel clock from the microscope, achieving pixel-to-pixel focus-switching. The capability of the imaging system is demonstrated by performing real-time multidepth two-photon fluorescence (TPF) and second-harmonic generation (SHG) imaging of freshly excised mouse lung lobes. TPF and SHG images are acquired at two wavelength ranges. One is between 415 and 455 nm, and the other is between 495 and 635 nm. The microenvironment of pulmonary alveoli is depicted by the distributions of both elastin fibers visualized by TPF and collagen fibers illustrated by SHG. Macrophages residing inside apparent alveolar lumens are also identified by TPF, which shows that the imaging system is capable of localizing biological objects in three dimensions and has the potential of monitoring in vivo cellular dynamics in the axial direction.

A disposable label-free electrochemiluminescent immunosensor for transferrin detection based on a luminol-reduced gold nanoparticle-modified screen-printed carbon electrode

2014 ◽  
Vol 6 (9) ◽  
pp. 2959-2964 ◽  
Author(s):  
Weijun Kong ◽  
He Zhou ◽  
Hui Ouyang ◽  
Zongyun Li ◽  
Zhifeng Fu
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Carbon Electrode ◽  
Label Free ◽  
Human Transferrin ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

A disposable label-free electrochemiluminescent immunosensor based on luminol-reduced gold nanoparticles was designed for human transferrin detection.

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