scholarly journals In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1906
Author(s):  
Mona Atabakhshi-Kashi ◽  
Mónica Carril ◽  
Hossein Mahdavi ◽  
Wolfgang J. Parak ◽  
Carolina Carrillo-Carrion ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Hydrophobic Interactions ◽  
Intrinsic Properties ◽  
Wide Range ◽  
Self Assembled ◽  
Uptake Studies ◽  
Targeting Ability ◽  
Fluorinated Nanoparticles

Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly).

scholarly journals Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 555 ◽  
Author(s):  
Pei Zhang ◽  
Shirui Zhao ◽  
Yaoyao Yu ◽  
Huan Wang ◽  
Yan Yang ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Polymeric Nanoparticles ◽  
Cell Permeability ◽  
Intestinal Epithelial ◽  
Cellular Delivery ◽  
Concentration Time ◽  
Self Assembled ◽  
Uptake Studies ◽  
Cellular Uptake Efficiency

Polymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characterized in our lab. However, little is known about their interaction with cells and other biological systems. In this study, nanoparticles (NPs) based on PAE-Alg conjugates (PEA-NPs) with different degree of substitution (DS) were prepared and investigated. Our results showed that PEA-NPs had no effects on the proliferation of the human intestinal epithelial Caco-2 cells at concentrations up to 1000 μg/mL. Furthermore, the in vitro cellular uptake profile of PEA-NPs, concerning several parameters involved in the application of therapeutic or diagnostic NPs, such as NPs concentration, time and temperature, was described. Different NPs have been adopted for cellular uptake studies and the NPs internalized into Caco-2 cells were quantified. Cellular uptake efficiency could reach 60% within 4 h. PEA-NPs also showed greater cell permeability than oleoyl alginate ester nanoparticles (OAE-NPs) previously prepared in our lab. Our studies reveal that NPs based on PEA conjugate are promising nanosystems for cellular delivery.

A Novel Flow-Cytometry-Based Assay for Cellular Uptake Studies of Polyelectrolyte Microcapsules

Small ◽  
2008 ◽  
Vol 4 (10) ◽  
pp. 1763-1768 ◽  
Author(s):  
Maximilian Semmling ◽  
Oliver Kreft ◽  
Almudena Muñoz Javier ◽  
Gleb B. Sukhorukov ◽  
Josef Käs ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Polyelectrolyte Microcapsules ◽  
Uptake Studies

scholarly journals Cancer-targeted Controlled Delivery of Chemotherapeutic Anthracycline DerivativesUsing Apoferritin Nanocage Carriers

2020 ◽  
Author(s):  
Katarzyna Kurzatkowska ◽  
Manuel A. Pazos II ◽  
Jason I. Herschkowitz ◽  
Maria Hepel
Keyword(s):  
Hydrophobic Interactions ◽  
In Vitro Cytotoxicity ◽  
Controlled Delivery ◽  
Polar Interactions ◽  
Targeting Ability ◽  
Apoptotic Activity ◽  
Supramolecular Ensemble ◽  
Mcf10a Cells ◽  
Toxic Drugs

The interactions of chemotherapeutic drugs with nanocage protein apoferritin (APO) are the key features in the effective encapsulation and release of highly toxic drugs in APO-based controlled drug delivery systems. The encapsulation enables mitigating the drugs side effects, collateral damage to healthy cells, and adverse immune reactions. Herein, the interactions of anthracycline drugs with APO were studied to assess the effect of drug lipophilicity on their encapsulation excess n and in vitro activity. Anthracycline drugs, including doxorubicin (DOX), epirubicin (EPI), daunorubicin (DAU), and idarubicin (IDA), with lipophilicity P from 0.8 to 15, were investigated. We have found that in addition to hydrogen-bonded supramolecular ensemble formation with n = 24, there are two other competing contributions that enable increasing n under strong polar interactions (APO(DOX)) or under strong hydrophobic interactions (APO(IDA) of the highest efficacy). The encapsulation/release processes were investigated using UV-Vis, fluorescence, circular dichroism, and FTIR spectroscopies. In vitro cytotoxicity/growth inhibition tests and flow cytometry corroborate high apoptotic activity of APO(drugs) against targeted MDA-MB-231 adenocarcinoma and HeLa cancer cells, and low activity against non-tumorigenic MCF10A cells, demonstrating targeting ability of nanodrugs. A model for molecular interactions between anthracyclines and APO nanocarriers was developed, and the relationships derived compared with experimental results.

scholarly journals Synthesis and Characterization of pH-Sensitive Inulin Conjugate of Isoniazid for Monocyte-Targeted Delivery

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 555 ◽  
Author(s):  
Franklin Afinjuomo ◽  
Thomas G. Barclay ◽  
Ankit Parikh ◽  
Rosa Chung ◽  
Yunmei Song ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Targeted Delivery ◽  
Ph Sensitive ◽  
Proton Nuclear Magnetic Resonance ◽  
Antitubercular Drugs ◽  
Intracellular Targeting ◽  
Intracellular Drug Delivery ◽  
Uptake Studies ◽  
Endocytic Compartments

The use of particles for monocyte-mediated delivery could be a more efficient strategy and approach to achieve intracellular targeting and delivery of antitubercular drugs to host macrophages. In this study, the potential of inulin microparticles to serve as a drug vehicle in the treatment of chronic tuberculosis using a monocytes-mediated drug targeting approach was evaluated. Isoniazid (INH) was conjugated to inulin via hydrazone linkage in order to obtain a pH-sensitive inulin-INH conjugate. The conjugate was then characterized using proton nuclear magnetic resonance (1HNMR), Fourier transform infrared spectroscopy (FTIR) as well as in vitro, cellular uptake and intracellular Mycobacterium tuberculosis (Mtb) antibacterial efficacy. The acid-labile hydrazone linkage conferred pH sensitivity to the inulin-INH conjugate with ~95, 77 and 65% of the drug released after 5 h at pH 4.5, 5.2, and 6.0 respectively. Cellular uptake studies confirm that RAW 264.7 monocytic cells efficiently internalized the inulin conjugates into endocytic compartments through endocytosis. The intracellular efficacy studies demonstrate that the inulin conjugates possess a dose-dependent targeting effect against Mtb-infected monocytes. This was through efficient internalization and cleavage of the hydrazone bond by the acidic environment of the lysosome, which subsequently released the isoniazid intracellularly to the Mtb reservoir. These results clearly suggest that inulin conjugates can serve as a pH-sensitive intracellular drug delivery system for TB treatment.

Synthesis, characterization and preliminary in vitro cellular uptake studies of 67Ga(III) thiosemicarbazones

2013 ◽  
Vol 33 ◽  
pp. 154-157 ◽  
Author(s):  
Tawfeeq Ismail ◽  
Daniel D. Rossouw ◽  
Philip Beukes ◽  
Jacobus P. Slabbert ◽  
Gregory S. Smith
Keyword(s):  
Cellular Uptake ◽  
Uptake Studies

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

scholarly journals Biotin-Decorated PAMAM G4.5 Dendrimer Nanoparticles to Enhance the Delivery, Anti-Proliferative, and Apoptotic Effects of Chemotherapeutic Drug in Cancer Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 443 ◽  
Author(s):  
Endiries Yibru Hanurry ◽  
Tefera Worku Mekonnen ◽  
Abegaz Tizazu Andrgie ◽  
Haile Fentahun Darge ◽  
Yihenew Simegniew Birhan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Viability ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Cellular Uptake ◽  
Drug Loading ◽  
In Vitro Cytotoxicity ◽  
Amide Bond ◽  
Solid Cancer

Biotin receptors are overexpressed by various types of solid cancer cells and play a significant role in tumor metabolism, growth, and metastasis. Thus, targeting the biotin receptors on tumor cells may enhance the efficiency and reduce the side-effects of chemotherapy. The aim of this study was to develop a biotin-coupled poly(amido)amine (PAMAM) (PG4.5) dendrimer nanoparticle to enhance the tumor-specific delivery and intracellular uptake of anticancer drugs via receptor-mediated endocytosis. We modified PG4.5 with diethylenetriamine (DETA) followed by biotin via an amide bond and characterized the resulting PG4.5-DETA-biotin nanoparticles by 1H NMR, FTIR, and Raman spectroscopy. Loading and releasing of gemcitabine (GEM) from PG4.5-DETA-biotin were evaluated by UV–Visible spectrophotometry. Cell viability and cellular uptake were examined by MTT assay and flow cytometry to assess the biocompatibility, cellular internalization efficiency and antiproliferative activity of PG4.5-DETA-biotin/GEM. Gemcitabine-loaded PG4.5-DETA-biotin nanoparticles were spherical with a particle size of 81.6 ± 6.08 nm and zeta potential of 0.47 ± 1.25 mV. Maximum drug-loading content and encapsulation efficiency were 10.84 ± 0.16% and 47.01 ± 0.71%, respectively. Nearly 60.54 ± 1.99% and 73.96 ± 1.14% of gemcitabine was released from PG4.5-DETA-biotin/GEM nanoparticles after 48 h at the acidic pH values of 6.5 and 5, respectively. Flow cytometry and fluorescence microscopy of cellular uptake results revealed PG4.5-DETA-biotin/GEM nanoparticles selectively targeted cancer cells in vitro. Cytotoxicity assays demonstrated gemcitabine-loaded PG4.5-DETA-biotin significantly reduced cell viability and induced apoptosis in HeLa cells. Thus, biotin-coupled PG4.5-DETA nanocarrier could provide an effective, targeted drug delivery system and selectively convey gemcitabine into tumor cells.

Diblock- and triblock-copolymer based mixed micelles with high tumor penetration in vitro and in vivo

2016 ◽  
Vol 4 (19) ◽  
pp. 3216-3224 ◽  
Author(s):  
Xi Cao ◽  
Xu Zhou ◽  
Yu Wang ◽  
Tao Gong ◽  
Zhi-Rong Zhang ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Triblock Copolymer ◽  
Mixed Micelles ◽  
Tumor Spheroid ◽  
Tumor Penetration ◽  
Self Assembled

A series of self-assembled mixed micelles composed of TPGS and Pluronics were fabricated and their cellular uptake and exocytosis behaviors were studied in 2D cell and 3D tumor spheroid models.

scholarly journals In-vitro cytotoxicity and cellular uptake studies of luminescent functionalized core-shell nanospheres

2017 ◽  
Vol 24 (6) ◽  
pp. 1392-1403 ◽  
Author(s):  
Anees A. Ansari ◽  
T.N. Hasan ◽  
N.A. Syed ◽  
J.P. Labis ◽  
Ali A. Alshatwi
Keyword(s):  
Cellular Uptake ◽  
In Vitro Cytotoxicity ◽  
Core Shell ◽  
Uptake Studies

Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells

2015 ◽  
Vol 396 (11) ◽  
pp. 1255-1264 ◽  
Author(s):  
Dajana Lichtenstein ◽  
Johanna Ebmeyer ◽  
Patrick Knappe ◽  
Sabine Juling ◽  
Linda Böhmert ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cellular Uptake ◽  
In Vitro Digestion ◽  
Intestinal Cells ◽  
Digestion Process ◽  
X Ray Scattering ◽  
Food Components ◽  
Uptake Studies

Abstract Because of the rising application of nanoparticles in food and food-related products, we investigated the influence of the digestion process on the toxicity and cellular uptake of silver nanoparticles for intestinal cells. The main food components – carbohydrates, proteins and fatty acids – were implemented in an in vitro digestion process to simulate realistic conditions. Digested and undigested silver nanoparticle suspensions were used for uptake studies in the well-established Caco-2 model. Small-angle X-ray scattering was used to estimate particle core size, size distribution and stability in cell culture medium. Particles proved to be stable and showed radii from 3.6 to 16.0 nm. Undigested particles and particles digested in the presence of food components were comparably taken up by Caco-2 cells, whereas the uptake of particles digested without food components was decreased by 60%. Overall, these findings suggest that in vivo ingested poly (acrylic acid)-coated silver nanoparticles may reach the intestine in a nanoscaled form even if enclosed in a food matrix. While appropriate for studies on the uptake into intestinal cells, the Caco-2 model might be less suited for translocation studies. Moreover, we show that nanoparticle digestion protocols lacking food components may lead to misinterpretation of uptake studies and inconclusive results.

Sign in / Sign up

Export Citation Format

Share Document