scholarly journals Er3+-Ions-Doped Multiscale Nanoprobes for Fluorescence Imaging in Cellular and Living Mice

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2676
Author(s):  
Cong Cao ◽  
Yu Xie ◽  
Shi-Wen Li ◽  
Chang Hong
Keyword(s):  
Light Scattering ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Diagnostic Techniques ◽  
Single Nanoparticle ◽  
Nir Fluorescence ◽  
Nir Luminescence

With the development of biotechnology, luminescent nanoprobes for biological disease detection are widely used. However, the further application in clinic is limited by the reduced penetration depth in the tissues and light scattering. In this work, we have synthesized NaYF4:Yb,Er,Ce@SiO2-OAlg nanomaterials, which have both upconversion and near-infrared (NIR) luminescence. The optimized probes were determined to achieve cell imaging by its upconversion (UCL) luminescence and in vivo imaging through collection of NIR fluorescence signals simultaneously. The research is conducive to developing accurate diagnostic techniques based on UCL and NIR fluorescence imaging by a single nanoparticle.

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

Stable Twisted Conformation Aza-BODIPY NIR-II Fluorescent Nanoparticles with Ultra-large Stokes Shift for Imaging-Guided Phototherapy

2022 ◽  
Author(s):  
Youliang Tian ◽  
Huiting Zhou ◽  
Quan Cheng ◽  
Huiping Dang ◽  
Hongyun Qian ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Great Promise ◽  
Deep Penetration ◽  
Fluorescent Nanoparticles ◽  
Spatiotemporal Resolution ◽  
Large Stokes Shift

Fluorescence imaging in the second near-infrared window (NIR-II, 1000–1700 nm) holds great promise for in vivo imaging and imaging-guided phototherapy with deep penetration and high spatiotemporal resolution. It is very...

Near-infrared Emitting Gold-silver Nanoclusters with Large Stokes Shift for Two-Photon in Vivo Imaging

2021 ◽  
Author(s):  
Yaowei Peng ◽  
Xiaoyu Huang ◽  
Fu Wang
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Silver Nanoclusters ◽  
One Pot ◽  
Two Photon ◽  
Nir Fluorescence ◽  
Gold Silver ◽  
Stokes Shifts

Near-infrared emitting bi-metallic gold/silver nanoclusters with great stokes shifts were manufactured through one-pot synthesis. The gold/silver nanoclusters exhibit strong NIR fluorescence due to the silver effect, which can be applied...

scholarly journals Multimodal Mn-doped I–III–VI quantum dots for near infrared fluorescence and magnetic resonance imaging: from synthesis to in vivo application

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 9264-9272 ◽  
Author(s):  
Gary Sitbon ◽  
Sophie Bouccara ◽  
Mariana Tasso ◽  
Aurélie Francois ◽  
Lina Bezdetnaya ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Quantum Dots ◽  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Resonance Imaging ◽  
Near Infrared Fluorescence ◽  
Nir Fluorescence ◽  
Mn Doped

Cadmium-free quantum dots doped with Mn2+ions show promising results forin vivobimodal MRI and NIR fluorescence imaging.

A fluorinated aza-BODIPY derivative for NIR fluorescence/PA/19F MR tri-modality in vivo imaging

2019 ◽  
Vol 55 (42) ◽  
pp. 5851-5854 ◽  
Author(s):  
Lianhua Liu ◽  
Yaping Yuan ◽  
Yuqi Yang ◽  
Michael T. McMahon ◽  
Shizhen Chen ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Small Molecule ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Near Infrared Fluorescence ◽  
Highly Efficient ◽  
Nir Fluorescence

A fluorinated aza-BODIPY derivative BDPF was developed as a small molecule contrast agent, which displayed highly efficient near infrared fluorescence/photoacoustic/19F MR tri-modality tumor imaging.

scholarly journals Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study

2012 ◽  
Vol 6 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Toru Funayama ◽  
Masataka Sakane ◽  
Tetsuya Abe ◽  
Isao Hara ◽  
Eiichi Ozeki ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Rat Model ◽  
Near Infrared ◽  
Imaging Modality ◽  
Intraoperative Imaging ◽  
Spinal Metastasis ◽  
Vena Cava ◽  
Nir Fluorescence

Marginal resection during resection of a spinal metastasis is frequently difficult because of the presence of important tissues such as the aorta, vena cava, and dura mater, including the spinal cord adjacent to the vertebral body. Thus, there is an urgent need for novel intraoperative imaging modalities with the ability to clearly identify bone metastasis. We have proposed a novel nanocarrier loaded with indocyanine green (ICG) (ICG-lactosome) with tumor selectivity attributable to its enhanced permeation and retention (EPR) effect. We studied its feasibility in intraoperative near-infrared (NIR) fluorescence diagnosis with ICG-lactosome for imaging spinal metastasis. A rat model of subcutaneous mammary tumor and a rat model of spinal metastasis of breast cancer were used. Fluorescence emitted by the subcutaneous tumors and the spinal metastasis were clearly detected for at least 24 h. Moreover, imaging of the dissected spine revealed clear fluorescence emitted by the metastatic lesion in the L6 vertebra while the normal bone lacked fluorescence. This study was the first report on NIR fluorescence imaging of spinal metastasis in vivo. NIR fluorescence imaging with ICG-lactosome could be an effective intraoperative imaging modality for detecting spinal metastasis.

scholarly journals Perfusion Patterns in Patients with Chronic Limb-Threatening Ischemia versus Control Patients Using Near-Infrared Fluorescence Imaging with Indocyanine Green

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1417
Author(s):  
Pim Van Den Hoven ◽  
Lauren N. Goncalves ◽  
Paulus H. A. Quax ◽  
Catharina S. P. Van Rijswijk ◽  
Jan Van Schaik ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Tissue Perfusion ◽  
Arterial Disease ◽  
Diagnostic Techniques ◽  
Control Group ◽  
Maximum Slope ◽  
Perfusion Studies ◽  
Nir Fluorescence

In assessing the severity of lower extremity arterial disease (LEAD), physicians rely on clinical judgements supported by conventional measurements of macrovascular blood flow. However, current diagnostic techniques provide no information about regional tissue perfusion and are of limited value in patients with chronic limb-threatening ischemia (CLTI). Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) has been used extensively in perfusion studies and is a possible modality for tissue perfusion measurement in patients with CLTI. In this prospective cohort study, ICG NIR fluorescence imaging was performed in patients with CLTI and control patients using the Quest Spectrum Platform® (Middenmeer, The Netherlands). The time–intensity curves were analyzed using the Quest Research Framework. Fourteen parameters were extracted. Successful ICG NIR fluorescence imaging was performed in 19 patients with CLTI and in 16 control patients. The time to maximum intensity (seconds) was lower for CLTI patients (90.5 vs. 143.3, p = 0.002). For the inflow parameters, the maximum slope, the normalized maximum slope and the ingress rate were all significantly higher in the CLTI group. The inflow parameters observed in patients with CLTI were superior to the control group. Possible explanations for the increased inflow include damage to the regulatory mechanisms of the microcirculation, arterial stiffness, and transcapillary leakage.

scholarly journals Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

2018 ◽  
Vol 115 (17) ◽  
pp. 4465-4470 ◽  
Author(s):  
Jessica A. Carr ◽  
Daniel Franke ◽  
Justin R. Caram ◽  
Collin F. Perkinson ◽  
Mari Saif ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Emission Spectra ◽  
Nir Fluorescence ◽  
Near Infrared Dye ◽  
Silicon Based ◽  
Shortwave Infrared

Fluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000–2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Even though their emission spectra peak in the NIR, these dyes outperform commercial SWIR fluorophores and can be imaged in the SWIR, even beyond 1,500 nm. We show real-time fluorescence imaging using ICG at clinically relevant doses, including intravital microscopy, noninvasive imaging in blood and lymph vessels, and imaging of hepatobiliary clearance, and show increased contrast compared with NIR fluorescence imaging. Furthermore, we show tumor-targeted SWIR imaging with IRDye 800CW-labeled trastuzumab, an NIR dye being tested in multiple clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide-based SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications. Indeed, our findings suggest that emerging SWIR-fluorescent in vivo contrast agents should be benchmarked against the SWIR emission of ICG in blood.

scholarly journals Facile synthesis of β-lactoglobulin capped Ag2S quantum dots for in vivo imaging in the second near-infrared biological window

2016 ◽  
Vol 4 (37) ◽  
pp. 6271-6278 ◽  
Author(s):  
Jun Chen ◽  
Yifei Kong ◽  
Yan Wo ◽  
Hongwei Fang ◽  
Yunxia Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Infrared Region ◽  
Facile Synthesis ◽  
Near Infrared Region ◽  
Ag2s Quantum Dots ◽  
Β Lactoglobulin

Effectivein vivofluorescence imaging based on β-LG-Ag2S quantum dots at the second near-infrared region.

scholarly journals IRDye78 Conjugates for Near-Infrared Fluorescence Imaging

2002 ◽  
Vol 1 (4) ◽  
pp. 153535002002213
Author(s):  
Atif Zaheer ◽  
Thomas E. Wheat ◽  
John V. Frangioni
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Low Molecular Weight ◽  
Mass Ratio ◽  
Nir Fluorescence ◽  
Sample Purification ◽  
The Stability ◽  
Near Infrared Fluorescence Imaging ◽  
Neutral Conditions

The detection of human malignancies by near-infrared (NIR) fluorescence will require the conjugation of cancer-specific ligands to NIR fluorophores that have optimal photoproperties and pharmacokinetics. IRDye78, a tetra-sulfonated heptamethine indocyanine NIR fluorophore, meets most of the criteria for an in vivo imaging agent, and is available as an N-hydroxysuccinimide ester for conjugation to low-molecular-weight ligands. However, IRDye78 has a high charge-to-mass ratio, complicating purification of conjugates. It also has a potentially labile linkage between fluorophore and ligand. We have developed an ion-pairing purification strategy for IRDye78 that can be performed with a standard C18 column under neutral conditions, thus preserving the stability of fluorophore, ligand, and conjugate. By employing parallel evaporative light scatter and absorbance detectors, all reactants and products are identified, and conjugate purity is maximized. We describe reversible and irreversible conversions of IRDye78 that can occur during sample purification, and describe methods for preserving conjugate stability. Using seven ligands, spanning several classes of small molecules and peptides (neutral, charged, and/or hydrophobic), we illustrate the robustness of these methods, and confirm that IRDye78 conjugates so purified retain bioactivity and permit NIR fluorescence imaging of specific targets.

Sign in / Sign up

Export Citation Format

Share Document