Chondroprotective Effects of a Standardized Extract (KBH-JP-040) from Kalopanax pictus, Hericium erinaceus, and Astragalus membranaceus in Experimentally Induced In Vitro and In Vivo Osteoarthritis Models

Cataracts, one of the leading causes of preventable blindness worldwide, refers to lens degradation that is characterized by clouding, with consequent blurry vision. As life expectancies improve, the number of people affected with cataracts is predicted to increase worldwide, especially in low-income nations with limited access to surgery. Although cataract surgery is considered safe, it is associated with some complications such as retinal detachment, warranting a search for cheap, pharmacological alternatives to the management of this ocular disease. The lens is richly endowed with a complex system of non-enzymatic and enzymatic antioxidants which scavenge reactive oxygen species to preserve lens proteins. Depletion and/or failure in this primary antioxidant defense system contributes to the damage observed in lenticular molecules and their repair mechanisms, ultimately causing cataracts. Several attempts have been made to counteract experimentally induced cataract using in vitro, ex vivo, and in vivo techniques. The majority of the anti-cataract compounds tested, including plant extracts and naturally-occurring compounds, lies in their antioxidant and/or free radical scavenging and/or anti-inflammatory propensity. In addition to providing an overview of the pathophysiology of cataracts, this review focuses on the role of various categories of natural and synthetic compounds on experimentally-induced cataracts.

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Hericerin derivatives from Hericium erinaceus exert BDNF-like neurotrophic activity in central hippocampal neurons and enhance memory

10.1101/2020.08.28.271676 ◽

2020 ◽

Author(s):

Ramón Martínez-Mármol ◽

YeJin Chai ◽

Zahra Khan ◽

Seon Beom Kim ◽

Seong Min Hong ◽

...

Keyword(s):

Recognition Memory ◽

Hippocampal Neurons ◽

Peripheral Nerve Regeneration ◽

Neurotropic Activity ◽

Biologically Active ◽

Hericium Erinaceus ◽

Neurotrophic Activity ◽

Trkb Receptors

AbstractThe traditional medicinal mushroom Hericium erinaceus has long been known for enhancing the peripheral nerve regeneration through targeting nerve growth factor (NGF) neurotrophic activity. It was also reported to protect against ageing-dependent cognitive decline in wildtype and in Alzheimer’s disease mouse models suggesting a yet to be defined action on neurons of the central nervous system. Here, we purified and identified biologically active compounds from H. erinaceus, based on their ability to promote neurite outgrowth in hippocampal neurons. N-de phenylethyl isohericerin (NDPIH), an isoindoline compound from this mushroom together with its hydrophobic derivative hericene A, were highly potent in inducing extensive axon outgrowth and neurite branching in the absence of serum demonstrating high neurotropic activity. NDPIH also induced enlarged growth cones suggestive of a brain-derived neurotrophic factor (BDNF)-like activity. Pharmacological inhibition of tropomyosin receptor kinase B (TrkB) by ANA12 prevented NDPIH-induced neurotrophic activity providing evidence that NDPIH acts via TrkB receptors to mediate its neurotrophic effect in central neurons. Finally, in vivo treatment with H. erinaceus crude extract and hericene A significantly increased BDNF and downstream pathway and enhanced learning and memory in the novel object recognition memory test. Our results suggest that hericene A can promote BDNF-like activity in neurons in vitro and in vivo thereby enhancing recognition memory.

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The use of Hericium erinaceus and Trametes versicolor extracts in supportive treatment in oncology

Acta Pharmaceutica ◽

10.2478/acph-2021-0007 ◽

2021 ◽

Vol 71 (1) ◽

pp. 1-16

Author(s):

Mateusz Winder ◽

Weronika Bulska-Będkowska ◽

Jerzy Chudek

Keyword(s):

Trametes Versicolor ◽

Therapeutic Effects ◽

Immunomodulatory Effects ◽

Edible Fungi ◽

Hericium Erinaceus ◽

Natural Medicine ◽

The Subject ◽

New Challenges

AbstractSubstances available in nature with potential therapeutic effects are the subject of research that raises tremendous hopes for new challenges in medicine. Fungi are the most common organisms in the ecosystem and the most interesting in this respect. This review discusses two species of edible fungi, used for centuries in Eastern natural medicine, with the best-documented effect – Hericium erinaceus (He) and Trametes versicolor (Tv). The results of in vivo and in vitro studies conducted on mice and human cell lines demonstrate immunomodulatory, potentially, anticancer, anti-inflammatory and neuroregenerative effects of substances isolated from these fungi. The substances contained in the extracts of He and Tv seem to have immunomodulatory effects that may support chemotherapy. The use of these extracts is justified stronger than the other supportive treat ments based on supplements.

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Experimentally induced hip dislocation dislocation in vitro and in vivo

Acta Orthopaedica Scandinavica ◽

10.3109/17453678309154168 ◽

1983 ◽

Vol 54 (sup199) ◽

pp. 1-57 ◽

Cited By ~ 4

Author(s):

Stefan Asplund ◽

Åke Hjelmstedt

Keyword(s):

Hip Dislocation ◽

Experimentally Induced

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Aquaporin 1 (AQP1) expression in experimentally induced osteoarthritic knee menisci: An in vivo and in vitro study

Tissue and Cell ◽

10.1016/j.tice.2012.10.004 ◽

2013 ◽

Vol 45 (2) ◽

pp. 145-152 ◽

Cited By ~ 32

Author(s):

G. Musumeci ◽

R. Leonardi ◽

M.L. Carnazza ◽

V. Cardile ◽

K. Pichler ◽

...

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Osteoarthritic Knee ◽

Aquaporin 1 ◽

Aqp1 Expression ◽

Experimentally Induced

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Astragalus (Astragalus membranaceus Bunge): botanical, geographical, and historical aspects to pharmaceutical components and beneficial role

RENDICONTI LINCEI ◽

10.1007/s12210-021-01003-2 ◽

2021 ◽

Author(s):

Alessandra Durazzo ◽

Amirhossein Nazhand ◽

Massimo Lucarini ◽

Amelia M. Silva ◽

Selma B. Souto ◽

...

Keyword(s):

Medicinal Plants ◽

Therapeutic Potential ◽

Folk Medicine ◽

Astragalus Membranaceus ◽

Special Focus ◽

Comprehensive Overview ◽

Historical Aspects ◽

Therapeutic Properties

AbstractMedicinal plants always are part of folk medicine and are nowadays receiving worldwide attention for prophylaxis, management, and treatment of several diseases, as an alternative to chemical drugs. The current work provided a comprehensive overview and analysis of the Astragalus and health relationship in literature. The analysis of their therapeutic potential is thus instrumental to understand their bioactivity. Among these, the flowering medicinal plant Astragalus membranaceus has raised interest due to several beneficial health effects. This perspective review discussed the botanical, geographical, historical, and the therapeutic properties of A. membranaceus, with a special focus on its health improving effects and medicinal applications both in vitro and in vivo. Graphic abstract

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Effects of ticagrelor, empagliflozin and tamoxifen against experimentally-induced vascular reactivity defects in rats in vivo and in vitro

Pharmacological Reports ◽

10.1016/j.pharep.2019.06.004 ◽

2019 ◽

Vol 71 (6) ◽

pp. 1034-1043 ◽

Cited By ~ 3

Author(s):

Yasmin Moustafa Ahmed ◽

Basim Anwar Shehata Messiha ◽

Mahmoud El-Sayed El-Daly ◽

Ali Ahmed Abo-Saif

Keyword(s):

Vascular Reactivity ◽

Experimentally Induced

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Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro

Experimental and Therapeutic Medicine ◽

10.3892/etm.2016.3152 ◽

2016 ◽

Vol 11 (5) ◽

pp. 1611-1616 ◽

Cited By ~ 9

Author(s):

GUANG SHAN ◽

XIANG-JUN ZHOU ◽

YUE XIA ◽

HUI-JUN QIAN

Keyword(s):

Epithelial Mesenchymal Transition ◽

Interstitial Fibrosis ◽

Astragalus Membranaceus ◽

Renal Interstitial Fibrosis ◽

Mesenchymal Transition

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Gyki 14,451, A Specific Inhibitor Of Thrombin:“In Vitro” And“In Vivo”Studies

10.1055/s-0038-1652079 ◽

1981 ◽

Author(s):

E Tremoli ◽

P Maderna ◽

S Colli ◽

G Morazzoni ◽

R Paoletti

Keyword(s):

Platelet Aggregation ◽

Venous Thrombosis ◽

Platelet Rich Plasma ◽

Vena Cava ◽

Specific Inhibitor ◽

Kinetic Studies ◽

In Vivo Studies ◽

Experimentally Induced

The effects of a synthetic tripeptide, Gyki 14,451(Boc-D Phe-Pro-Arg-H)have been studied in vitro on human platelet aggregation and arachidonic acid (AA) metabolism and in vivo on experimentally induced venous thrombosis in the rat.1 μM Gyki 14,451 concentration selectively inhibits thrombin induced platelet aggregation as well as malondial- dehyde (MDA) and thromboxane B2 (TXB2) formation by platelet rich plasma (PRP) stimulated with thrombin. Far higher concentrations (400μM)of the peptide are required to exert an inhibitory effect when collagen, ADP and AA are used to stimulate platelets. No effect has been observed on the conversion of 14C AA to metabolites using unstimulated platelets. Kinetic studies of MDA production by platelets stimulated with thrombin and its inhibition by Gyki 14,451 (0.15,0.3,0.6 uM) suggest that the peptide interacts with thrombin by an apparently competitive mechanism.4 mg/Kg of Gyki 14,451, intravenously injected in the rat caudal vein, completely inhibited the occurrence of venous thrombosis, obtained by vena cava ligature.The oral administration of the peptide (50 mg/Kg by gastric intubation) failed to reduce the percentage of incidence of venous thrombosis (88% in controls versus 90% in treated rats) resulting only in a reduction of the thrombus weight. These data suggest that the anticoagulant peptide Gyki 14,451, given intravenously, possesses a consistent activity in the prevention of experimentally induced venous thrombosis.

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