Dietary Acid Load and Its Interaction with IGF1 (rs35767 and rs7136446) and IL6 (rs1800796) Polymorphisms on Metabolic Traits among Postmenopausal Women

The objective of this study was to explore the effects of dietary acid load (DAL) and IGF1 and IL6 gene polymorphisms and their potential diet–gene interactions on metabolic traits. A total of 211 community-dwelling postmenopausal women were recruited. DAL was estimated using potential renal acid load (PRAL). Blood was drawn for biochemical parameters and DNA was extracted and Agena® MassARRAY was used for genotyping analysis to identify the signalling of IGF1 (rs35767 and rs7136446) and IL6 (rs1800796) polymorphisms. Interactions between diet and genetic polymorphisms were assessed using regression analysis. The result showed that DAL was positively associated with fasting blood glucose (FBG) (β = 0.147, p < 0.05) and there was significant interaction effect between DAL and IL6 with systolic blood pressure (SBP) (β = 0.19, p = 0.041). In conclusion, these findings did not support the interaction effects between DAL and IGF1 and IL6 single nucleotide polymorphisms (rs35767, rs7136446, and rs1800796) on metabolic traits, except for SBP. Besides, higher DAL was associated with higher FBG, allowing us to postulate that high DAL is a potential risk factor for diabetes.

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Interaction Between Dietary Acid Load and IL6 -572 G/C Polymorphism on Blood Pressure Among Postmenopausal Women in Malaysia

Current Developments in Nutrition ◽

10.1093/cdn/nzaa058_022 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1264-1264

Author(s):

Sook Yee Lim ◽

Yoke Mun Chan ◽

Ramachandran Vasudevan ◽

Mohd Shariff Zalilah ◽

Yit Siew Chin

Keyword(s):

Blood Pressure ◽

Postmenopausal Women ◽

Positive Association ◽

Gene Interaction ◽

Community Dwelling ◽

Dietary Intakes ◽

Significant Positive Association ◽

Dietary Acid Load ◽

Dietary Acid ◽

Acid Load

Abstract Objectives We examined whether IL6 single nucleotide genetic polymorphism modified the association between dietary acid load (DAL) and blood pressure among postmenopausal women in Malaysia. Methods A total of 211 community-dwelling postmenopausal women were recruited. Dietary intakes of participants were assessed using a validated interview-administered semi-quantitative food frequency questionnaire while DAL was estimated using potential renal acid load (PRAL). Agena® MassARRAY genotyping analysis was used to identify the IL6 genotype and blood pressure was measured using a Digital Automatic BP monitor (OMRON HEM-907, Japan). Interaction between DAL and IL6 -572 G/C polymorphism was assessed using linear regression test. Results There was a significant interaction between DAL and IL6 -572 G/C polymorphism on systolic blood pressure (SBP) (Pinteraction = 0.041). A significant positive association between DAL and SBP with stronger relationship in CG and GG genotype carriers compare to CC carriers were observed. On the other hand, there was no significant diet-gene interaction effect on diastolic blood pressure. Conclusions Our findings suggest that the association between DAL and SBP might be influenced by IL6 -572 G/C polymorphism among postmenopausal women. Further work on how IL6 -572 G/C polymorphism influences the association with DAL on hypertension are warranted. Funding Sources Supported by Fundamental Research Grant Scheme (FRGS), Ministry of Higher Education Malaysia, and Putra Grant UPM.

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Associations between age-related changes in bone microstructure and strength and dietary acid load in a cohort of community-dwelling, healthy men and postmenopausal women

American Journal of Clinical Nutrition ◽

10.1093/ajcn/nqaa191 ◽

2020 ◽

Vol 112 (4) ◽

pp. 1120-1131

Author(s):

Maria Papageorgiou ◽

Fanny Merminod ◽

Thierry Chevalley ◽

Bert van Rietbergen ◽

Serge Ferrari ◽

...

Keyword(s):

Postmenopausal Women ◽

Bone Microstructure ◽

Community Dwelling ◽

Mineral Density ◽

Healthy Men ◽

Dietary Acid Load ◽

Age Related ◽

Dietary Acid ◽

Acid Load ◽

Age Related Changes

ABSTRACT Background The importance of dietary acid load (DAL) in the pathogenesis of osteoporosis is still debated. Age-related changes in bone microstructure and strength in relation to DAL remain largely unexplored. Objectives We investigated the associations between changes in areal and volumetric bone mineral density (BMD), bone microstructure and strength, fracture risk, and DAL in a prospective cohort of 65-y-old healthy men and postmenopausal women. Methods Potential renal acid load (PRAL; mEq/d) was calculated as a DAL proxy to characterize participants’ diet as alkaline (Alk-D; PRAL < −5), neutral (Neut-D; −5 ≤ PRAL ≤ 5), or acidic (Acid-D; PRAL >5). We measured areal BMD (aBMD) by DXA, and distal radius and tibia bone microstructure using high-resolution peripheral quantitative computed tomography, at baseline (n = 853) and after 6.1 ± 1.4 y (n = 708). Bone strength was estimated using finite element analyses at baseline and after 3.0 ± 0.5 y (n = 613). Prevalent and incident fractures were recorded. Results The majority of the participants (59%) had an Alk-D, while 23% had a Neut-D, and 18% an Acid-D. Baseline aBMD and bone microstructure and strength did differ or were slightly better in women or men with an Acid-D versus those consuming an Alk-D or Neut-D. Indeed, women with an Acid-D had higher trabecular number (P = 0.010 vs. Alk-D; P = 0.001 vs. Neut-D), while men had higher hip and radius aBMD (P = 0.008 and 0.024 vs. Neut-D, respectively) and radius strength (P = 0.026 vs. Neut-D). Over the follow-up, women in the Acid-D group experienced lower cortical and endocortical bone loss at the radius than did the Alk-D and Neut-D groups (cortical thickness, P = 0.008 and < 0.001; trabecular area, P = 0.001 and < 0.001, respectively). No association between fractures and PRAL was observed. Conclusions These null or favourable associations between baseline values or changes in aBMD, bone microstructure and strength, and DAL in this cohort of 65-y-old healthy individuals do not support adverse DAL-mediated effects on bone. This trial was registered at http://www.isrctn.com as ISRCTN11865958.

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No Interaction Effect between Interleukin-6 Polymorphisms and Acid Ash Diet with Bone Resorption Marker in Postmenopausal Women

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18020827 ◽

2021 ◽

Vol 18 (2) ◽

pp. 827

Author(s):

Sook Yee Lim ◽

Yoke Mun Chan ◽

Vasudevan Ramachandran ◽

Zalilah Mohd Shariff ◽

Yit Siew Chin ◽

...

Keyword(s):

Bone Resorption ◽

Postmenopausal Women ◽

Interaction Effect ◽

Interleukin 6 ◽

Gene Polymorphisms ◽

Bone Resorption Marker ◽

Dietary Intakes ◽

Dietary Acid Load ◽

Dietary Acid ◽

Acid Load

Background: Evidence is growing that a high-acid diet might accelerate the rate of bone loss, and gene polymorphisms such as Interleukin 6 (IL6) -174G/C and -572G/C are related to bone deterioration. However, no study of the interaction between diet and IL6 polymorphisms has been conducted among Asians. Thus, the objective of this study was to determine whether IL6 gene polymorphisms modified the association between dietary acidity and the rate of bone resorption. Methods: This cross-sectional study recruited 203 postmenopausal women (age ranged from 51 to 85 years old) in community settings. The dietary intakes of the participants were assessed using a validated interviewer-administered semi-quantitative food frequency questionnaire (FFQ), while dietary acid load (DAL) was estimated using net endogenous acid production (NEAP). Agena® MassARRAY genotyping analysis and serum collagen type 1 cross-linked C-telopeptide (CTX1) were used to identify the IL6 genotype and as a bone resorption marker, respectively. The interactions between diet and single-nucleotide polymorphisms (SNPs) were assessed using linear regressions. Results: A total of 203 healthy postmenopausal women aged between 51 and 85 years participated in this study. The mean BMI of the participants was 24.3 kg/m2. In IL6 -174 G/C, all the participants carried the GG genotype, while the C allele was absent. Approximately 40% of the participants had a high dietary acid load. Dietary acid load (B = 0.15, p = 0.031) and the IL6 -572 CC genotype group (B = 0.14, p = 0.044) were positively associated with a higher bone resorption. However, there was no moderating effect of the IL6 genetic polymorphism on the relationship between and acid ash diet and bone resorption markers among the postmenopausal women (p = 0.79). Conclusion: High consumption of an acid ash diet and the IL6 -572 C allele seem to attribute to high bone resorption among postmenopausal women. However, our finding does not support the interaction effect of dietary acidity and IL6 (-174G/C and -572G/C) polymorphisms on the rate of bone resorption. Taken together, these results have given scientific research other candidate genes to focus on which may interact with DAL on bone resorption, to enhance planning for preventing or delaying the onset of osteoporosis among postmenopausal women.

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Dietary Acid Load, IGF-1 Single Nucleotide Polymorphism and Bone Resorption among Postmenopausal Chinese Women

Nutrients ◽

10.3390/nu10070915 ◽

2018 ◽

Vol 10 (7) ◽

pp. 915 ◽

Cited By ~ 1

Author(s):

Sook Lim ◽

Mohd Zalilah ◽

Yit Chin ◽

Vasudevan Ramachandran ◽

Yoke Chan

Keyword(s):

Physical Activity ◽

Single Nucleotide Polymorphism ◽

Bone Resorption ◽

Chinese Women ◽

National Council ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Dietary Acid Load ◽

Dietary Acid ◽

Acid Load

The interaction of dietary and genetic factors may affect the development of bone deterioration. This study investigated whether the effects of dietary acid load (DAL) on bone loss in postmenopausal Chinese women were moderated by the insulin-like growth factor-1 (IGF-1) single nucleotide polymorphism, a known gene that plays a role in the regulation of bone formation and bone remodeling. A total of 217 healthy participants were recruited from the National Council of Senior Citizens Organizations Malaysia. Serum collagen type 1 cross-linked C-telopeptide was used as a surrogate bone marker to assess bone resorption and Agena® MassARRAY genotyping analysis was used to identify the signaling of IGF-1 rs35767. The dietary acid load was measured by potential renal acid load score while physical activity was ascertained using the Global Physical Activity Questionnaire. Hierarchical regression was applied to test the main and interaction effects of DAL and IGF-1 genotypes in bone resorption. The result supported the diet-dependent acid-base balance theory that higher DAL was positively associated with bone resorption (β = 0.152, p = 0.031, F(6,207) = 2.11, sig-F = 0.036, R2 = 0.079). However, the results indicated that there was no significant correlation between IGF-1 and bone resorption, or any significant interaction between DAL and IGF-1. In conclusion, there was no moderating effect of IGF-1 on the relationship between DAL and bone resorption.

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Association of Dietary Acid Load with the Prevalence of Metabolic Syndrome among Participants in Baseline Survey of the Japan Multi-Institutional Collaborative Cohort Study

Nutrients ◽

10.3390/nu12061605 ◽

2020 ◽

Vol 12 (6) ◽

pp. 1605

Author(s):

Kokichi Arisawa ◽

Sakurako Katsuura-Kamano ◽

Hirokazu Uemura ◽

Nguyen Van Tien ◽

Asahi Hishida ◽

...

Keyword(s):

Metabolic Syndrome ◽

Body Mass Index ◽

Cohort Study ◽

Body Mass ◽

Fasting Blood Glucose ◽

Carbohydrate Intake ◽

Baseline Survey ◽

Dietary Acid Load ◽

Dietary Acid ◽

Acid Load

The association between dietary acid load and metabolic syndrome (MetS) has not been fully investigated. A cross-sectional study was performed on 14,042 men and 14,105 women (aged 35–69 years) who participated in a baseline survey of the Japan Multi-Institutional Collaborative Cohort study. Dietary acid load was assessed using the net-endogenous-acid-production (NEAP) score that is closely correlated with the rate of renal net acid excretion. MetS was diagnosed according to the Joint Interim Statement Criteria of 2009 using body-mass index instead of waist circumference. After adjusting for potential confounders, higher NEAP scores were associated with a significantly increased odds ratio (OR) of MetS, obesity, high blood pressure, and high fasting blood glucose. These associations remained significant after further adjustment for carbohydrate intake or two nutrient-pattern scores significantly associated with MetS. After adjustment for fiber, iron, potassium, and vitamin pattern scores, the OR of MetS for the highest quartile of NEAP scores, relative to the lowest quartile, was 1.25 (95% confidence interval 1.12–1.39). There was no significant interaction between sex, age, or body-mass index and NEAP. Higher dietary acid load was associated with a higher prevalence of MetS and several of its components, independently of carbohydrate intake or nutrient patterns.

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Dietary acid load, insulin sensitivity and risk of type 2 diabetes in community-dwelling older men

Diabetologia ◽

10.1007/s00125-014-3275-z ◽

2014 ◽

Vol 57 (8) ◽

pp. 1561-1568 ◽

Cited By ~ 36

Author(s):

Hong Xu ◽

Ting Jia ◽

Xiaoyan Huang ◽

Ulf Risérus ◽

Tommy Cederholm ◽

...

Keyword(s):

Type 2 Diabetes ◽

Insulin Sensitivity ◽

Older Men ◽

Community Dwelling ◽

Dietary Acid Load ◽

Dietary Acid ◽

Acid Load

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Dietary Acid Load and Its Interaction With IGF1 and IL6 Polymorphisms on Metabolic Traits Among Postmenopausal Women

10.21203/rs.3.rs-129378/v1 ◽

2020 ◽

Author(s):

Sook Yee Lim ◽

Yoke Mun Chan (0000-0002-3853-736X) ◽

Vasudevan Ramachandran ◽

Zalilah Mohd Shariff ◽

Yit Siew Chin ◽

...

Keyword(s):

Blood Pressure ◽

Postmenopausal Women ◽

Genetic Polymorphisms ◽

Gene Polymorphisms ◽

Significant Interaction ◽

Cholesterol Ratio ◽

Metabolic Traits ◽

Dietary Acid ◽

Acid Load ◽

Il6 Gene

Abstract Background: Despite considerable work has been done to understand the underlying disease mechanisms by elucidating its genetic etiology, still the pathophysiology of the metabolic syndrome remains unclear. While numerous studies have shown that Insulin growth factors-1 (IGF1), Interleukin-6 (IL-6) gene polymorphisms and high dietary acid load (DAL) were related to undesirable profile of metabolic traits; it remains controversial whether DAL is associated with genetic polymorphisms or risk of metabolic traits. Thus, the objective of this study was to explore the effects of DAL, IGF1 and IL6 gene polymorphisms and their potential diet-gene interactions on metabolic traits. Methods: A total of 211 community-dwelling postmenopausal women were recruited. DAL was estimated using potential renal acid load (PRAL). Blood was drawn for biochemical parameters and DNA was extracted and Agena® MassARRAY was used for genotyping analysis to identify the signaling of IGF1 (rs35767, rs7136446) and IL6 (rs1800796) polymorphisms. Interactions between diet and genetic polymorphisms were assessed using hierarchical multiple linear regression analysis.Results: Mean age of respondents was 67 ± 6.7 years. A total of 68.2%, 59.2%, 49.3%, 23.2%, 23.2% and 10.9% had elevated systolic blood pressure (SBP), hyperglycemia, excessive waist circumference (WC), diastolic blood pressure (DBP), triglycerides (TG) and low high-density lipoprotein (HDL), respectively. DAL was positively associated with FBG (β = 0.147, p < 0.05) and there was significant interaction effect between DAL and IL6 with SBP (β = 0.19, p = 0.041). On the other hand, there were no significant main effects of DAL and single nucleotide polymorphisms (SNPs) (rs35767, rs7136446 and rs1800796) on SBP, DBP, WC, TG or HDL and cholesterol ratio. There were also no significant interaction effects between DAL and IGF1 SNPs (rs35767 and rs7136446) on SBP, DBP and FBG, TG, HDL and cholesterol ratio. Conclusion: These findings did not support the interaction effects between DAL and IGF1 and IL6 SNPs (s35767, rs7136446 and rs1800796) on metabolic traits, except for SBP. Besides, higher DAL was associated with higher FBG, allowing us to postulate high DAL is a potential risk factor for diabetes. Future research delineating foods that contribute to high DAL, and how IL6 gene polymorphism influences the association with DAL on hypertension are warranted.

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