scholarly journals A Citrus Fruit Extract High in Polyphenols Beneficially Modulates the Gut Microbiota of Healthy Human Volunteers in a Validated In Vitro Model of the Colon

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3915
Author(s):  
Mônica Maurer Sost ◽  
Sanne Ahles ◽  
Jessica Verhoeven ◽  
Sanne Verbruggen ◽  
Yala Stevens ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Citrus Fruit ◽  
Short Chain Fatty Acids ◽  
Overweight And Obesity ◽  
Fruit Extract ◽  
Healthy Human ◽  
Human Volunteers ◽  
Vitro Model

The effect of a Citrus Fruit Extract high in the polyphenols hesperidin and naringin (CFE) on modulation of the composition and activity of the gut microbiota was tested in a validated, dynamic in vitro model of the colon (TIM-2). CFE was provided at two doses (250 and 350 mg/day) for 3 days. CFE led to a dose-dependent increase in Roseburia, Eubacterium ramulus, and Bacteroides eggerthii. There was a shift in production of short-chain fatty acids, where acetate production increased on CFE, while butyrate decreased. In overweight and obesity, acetate has been shown to increase fat oxidation when produced in the distal gut, and stimulate secretion of appetite-suppressive neuropeptides. Thus, the data in the in vitro model point towards mechanisms underlying the effects of the polyphenols in CFE with respect to modulation of the gut microbiota, both in composition and activity. These results should be confirmed in a clinical trial.

In-vitro model for studying methanogens in human gut microbiota

Anaerobe ◽  
2015 ◽  
Vol 34 ◽  
pp. 50-52 ◽  
Author(s):  
William Tottey ◽  
Nadia Gaci ◽  
Guillaume Borrel ◽  
Monique Alric ◽  
Paul W. O'Toole ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Vitro Model

scholarly journals Xylo-oligosaccharides from sugarcane show prebiotic potential in a dynamic computer-controlled in vitro model of the adult human large intestine

2020 ◽  
Vol 11 (2) ◽  
pp. 191-200 ◽  
Author(s):  
K. Venema ◽  
J. Verhoeven ◽  
S. Verbruggen ◽  
D. Keller
Keyword(s):  
In Vitro Model ◽  
Short Chain Fatty Acids ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Gut Health ◽  
Unidentified Species ◽  
Human Adults ◽  
Computer Controlled ◽  
Vitro Model

The aim of the study was to investigate the prebiotic potential of xylo-oligosaccharides (XOS) from sugarcane in a validated, dynamic, computer-controlled in vitro model of the colon (TIM-2) simulating human adults. In two sets of experiments, each with a different microbiota, 3 different doses of XOS were tested at 1.0 g/day, 1.5 g/day and 3.0 g/day. The in vitro model was run for 72 h, and at the start and subsequently every 24 h samples were taken and analysed for short-chain fatty acids (SCFA) and gut microbiota composition. SCFA were analysed using ion chromatography, whereas microbiota composition was analysed using sequencing of the V3-V4 region of the 16S rRNA gene. XOS showed a similar SCFA production per gram of substrate as the control medium, including butyrate, which is considered to be important for gut health. In both sets of experiments XOS showed a consistent dose-dependent increase in abundance over time of the genus Bifidobacterium, and within that of the species B. adolescentis and an unidentified species (labelled ‘sp.1’). The results show the potential prebiotic effect of XOS from sugarcane, by its capacity to generate butyrate and increase the health-beneficial bifidobacteria.

scholarly journals In vitro model insights into the role of human gut microbiota on arsenic bioaccessibility and its speciation in soils

2020 ◽  
Vol 263 ◽  
pp. 114580 ◽  
Author(s):  
Haifeng Chi ◽  
Yanwei Hou ◽  
Guofeng Li ◽  
Youchi Zhang ◽  
Frédéric Coulon ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Vitro Model

scholarly journals Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon

2019 ◽  
Vol 10 (3) ◽  
pp. 301-313
Author(s):  
A. Martina ◽  
G.E. Felis ◽  
M. Corradi ◽  
C. Maffeis ◽  
S. Torriani ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Bacillus Coagulans ◽  
Proximal Colon ◽  
Gene Profiling ◽  
Rrna Gene ◽  
Dietary Interventions ◽  
Vitro Model ◽  
Microbial Groups

Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities. We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2). Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively. In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.

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