scholarly journals Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (Danio rerio)

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4379
Author(s):  
Hui Zhou ◽  
Yuanyuan Wu ◽  
Eunhye Kim ◽  
Haibo Pan ◽  
Puming He ◽  
...  
Keyword(s):  
Glucose Level ◽  
Danio Rerio ◽  
Hepg2 Cells ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Black Tea ◽  
Absorption Capacity ◽  
Hypoglycemic Effect ◽  
Human Hepatoma ◽  
Β Cells ◽  
Metabolism Enzymes

Theaflavin-3,3′-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish (Danio rerio) were used simultaneously to reveal anti-diabetic effect of TF3. The results showed that TF3 could effectively rise glucose absorption capacity in insulin-resistant HepG2 cells and regulate glucose level in diabetic zebrafish. The hypoglycemic effect was mediated through down-regulating phosphoenolpyruvate carboxykinase and up-regulating glucokinase. More importantly, TF3 could significantly improve β cells regeneration in diabetic zebrafish at low concentrations (5 μg/mL and 10 μg/mL), which meant TF3 had a strong anti-diabetic effect. Obviously, this work provided the potential benefit of TF3 on hypoglycemic effect, regulating glucose metabolism enzymes, and protecting β cells. TF3 might be a promising agent for combating diabetes.

MICROCHEMICAL ASSAYS OF GLUCOSE AND GLUCOSE-6-PHOSPHATE IN MAMMALIAN PANCREATIC β-CELLS

1968 ◽  
Vol 59 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Lars-Ake Idahl ◽  
Bo Hellman
Keyword(s):  
Glucose Level ◽  
Exocrine Pancreas ◽  
The Other ◽  
Wide Concentration Range ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Enzymatic Cycling ◽  
Glucose Diffusion ◽  
Significant Barrier ◽  
The Brain

ABSTRACT The combination of enzymatic cycling and fluorometry was used for measuring glucose and glucose-6-phosphate in pancreatic β-cells from obese-hyperglycaemic mice. The glucose level of the β-cells corresponded to that of serum over a wide concentration range. In the exocrine pancreas, on the other hand, a significant barrier to glucose diffusion across the cell membranes was demonstrated. During 5 min of ischaemia, the glucose level remained practically unchanged in the β-cells while it increased in the liver and decreased in the brain. The observation that the pancreatic β-cells are characterized by a relatively low ratio of glucose-6-phosphate to glucose may be attributed to the presence of a specific glucose-6-phosphatase.

Interaction of PIG11 and Hsp60 is Involved in Apoptosis in Human Hepatoma HepG2 Cells*

2010 ◽  
Vol 37 (10) ◽  
pp. 1138-1143
Author(s):  
Xiao-Min LIU ◽  
Rong HU ◽  
Xiao-Qiu LIANG ◽  
Xiao-Juan WANG ◽  
Yan WU ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

scholarly journals PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE

2018 ◽  
Vol 10 (1) ◽  
pp. 71
Author(s):  
Shibu Narayan Jana ◽  
Papiya Mitra Mazumder
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Cholesterol Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Fasting Blood Glucose Level ◽  
Β Cells ◽  
Partial Pancreatectomy ◽  
Swiss Albino Mice ◽  
Diabetes Status

Objective: The present study was aimed at the development of partial pancreatectomy in a murine model for diabetes.Methods: Diabetes model was successfully developed by partial pancreatectomy method. In this study, cyclosporine was used for influenced the glycaemic status. Diabetes status was evaluated by fasting blood glucose level (FBG), lipid profile (by estimation of total cholesterol level and HDL-level), serum amylase and biochemical assay like glutathione estimation.Results: We report here the restoration of euglycaemic status in cyclosporine-induced diabetic in swiss albino mice after 30% pancreatectomy. In this study, Pancreatectomised (partial) group of animals showed a rapid elevation of glycaemic status, starting from 15th post observational d, but the level of significance decreased gradually from 15th (P<0.01) to 60th (P<0.05) d. This was probably due to nesidioblastotic activity which shifted the fasting blood glucose level towards normoglycaemic status with β-cells regeneration. Cyclosporine treated a group of mice showed normoglycaemic status throughout the whole experimental period, but the cholesterol level remained significant (P<0.001) till the end of the experimental d. Gradually decrements in glycaemia of the diabetic pancreatectomised animals demonstrate islets neogenesis occurring after the operative activity, leading to normoglycaemic condition, probably attributed to β-cells proliferation.Conclusion: The biochemical and histopathological evaluations suggest that there is the development of the diabetic model in the pancreatectomized group and diabetes status induced by pancreatectomy is curable to a certain extent due to the regeneration of β-cells.

scholarly journals Gambogic acid suppresses cytochrome P450 3A4 by downregulating pregnane X receptor and up-regulating DEC1 in human hepatoma HepG2 cells

2015 ◽  
Vol 4 (4) ◽  
pp. 1059-1071 ◽  
Author(s):  
Wei Liu ◽  
Rui Ning ◽  
Rui-Ni Chen ◽  
Jin-Hua Hu ◽  
Hai-Yan Gui ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Hepg2 Cells ◽  
Pregnane X Receptor ◽  
Gambogic Acid ◽  
Cytochrome P450 3A4 ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

Gambogic acid suppresses cytochrome P450 3A4 by downregulating pregnane X receptor and up-regulating DEC1 in human hepatoma HepG2 cells.

Cytotoxic and genotoxic potential of Cr(VI), Cr(III)-nitrate and Cr(III)-EDTA complex in human hepatoma (HepG2) cells

Chemosphere ◽  
2016 ◽  
Vol 154 ◽  
pp. 124-131 ◽  
Author(s):  
Breda Novotnik ◽  
Janez Ščančar ◽  
Radmila Milačič ◽  
Metka Filipič ◽  
Bojana Žegura
Keyword(s):  
Hepg2 Cells ◽  
Human Hepatoma ◽  
Edta Complex ◽  
Genotoxic Potential ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2
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