scholarly journals Intermittent Fasting before Laparotomy: Effects on Glucose Control and Histopathologic Findings in Diabetic Rats

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4519
Author(s):  
André Keng Wei Hsu ◽  
Silvane Souza Roman ◽  
Margarete Dulce Bagatini ◽  
Filomena Marafon ◽  
Paulo do Nascimento Junior ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Perioperative Period ◽  
Diabetic Rats ◽  
Intermittent Fasting ◽  
Tubular Damage ◽  
Feeding Time ◽  
Surgical Trauma ◽  
Post Operative Period ◽  
Histopathologic Findings

(1) Background: Intermittent fasting is a nutrition practice in which individuals fast for several hours in a day, mainly with feeding time during the daylight hours. They seek to improve metabolic performance and cellular resistance to stress. In this study, we tested the fasting protocol to investigate the glycemic effect in a laparotomy perioperative period in diabetic rats and histopathologic findings. (2) Methods: The animals were diabetic-induced with alloxan. Two groups were set according to the feeding protocol: free food and intermittent fasting, whose rats could only eat 8 h in the daylight. Both groups were anesthetized, and a laparotomy was performed. We evaluated the glucose levels during the perioperative period, and we accessed organ histology seeking damage of kidney, bowel and liver after surgical trauma, and we evaluated the wound healing process. (3) Results: Glycemic levels were improved in the intermittent fasting group, especially in the post-operative period after laparotomy. Comparing both groups’ tubular damage showed interdependency with mice with worse glycemic level (Z = 2.3; p = 0.0215) and wound-healing parameters showed interdependency with rats with better glycemic status for neovascularization (Z = 2.2; p = 0.0273) and the presence of sebaceous and sweat gland in the healing process (Z = 2.30; p = 0.0215). (4) Conclusions: Intermittent fasting before surgery can be a tool to improve glycemic levels in diabetic rats, with improvement especially in the post-operative period.

Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

2020 ◽  
Vol 29 (9) ◽  
pp. 488-495
Author(s):  
Amene Nikgoftar Fathi ◽  
Mohammad Hassan Sakhaie ◽  
Sepehr Babaei ◽  
Soroush Babaei ◽  
Fateme Slimabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Wound Area ◽  
Small Vessels ◽  
Histological Sections ◽  
Therapeutic Benefits ◽  
Wide Range

Objective: To assess the effect of bromelain on different aspects of the wound healing process in type 1 diabetic rats. Method: In this study, 112 streptozocin-diabetic (type 1) male Wistar rats were euthanised; 28 each on days three, five, seven and 15, after a wound incision had been made. To estimate changes in a number of different cellular and tissue elements, histological sections were provided from all wound areas and stained with haematoxylin and eosin. Some 1.056mm2 of total wound area from all specimens were evaluated, by assessment of 4200 microscope photos provided from all histological sections, by stereological methods. A biomechanical test of each wound area was performed with an extensometer to evaluate the work-up to maximum force and maximum stress of the healed wound on day 15. Results: In the experimental groups, bromleain caused significant wound contraction and reduced granulation tissue formation by day 7 (p=0.003); increased neovasculars (new small vessels that appear in the wound area during wound healing) on days three, five and seven (p=0.001); significantly increased fibroblasts on day five but decreased by day seven (p=0.002); and significantly decreased macrophage numbers and epithelium thickness on all days of study (p=0.005). Wound strength significantly increased in experimental groups by day 15. Conclusion: Bromelain has a wide range of therapeutic benefits, but in most studies the mode of its action is not properly understood. It has been proved that bromelain has no major side effects, even after prolonged use. According to the results of this study, bromelain can be used as an effective health supplement to promote and accelerate wound healing indices, reduce inflammation and improve biomechanical parameters in diabetic wounds.

scholarly journals Ethanol extract ofCotinus coggygrialeaves accelerates wound healing process in diabetic rats

2016 ◽  
Vol 54 (11) ◽  
pp. 2732-2736 ◽  
Author(s):  
Halil Aksoy ◽  
Ali Sen ◽  
Mesut Sancar ◽  
Turgut Sekerler ◽  
Dilek Akakin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Wound Healing Process

Histological and clinical evaluation of wound healing in pressure ulcers: a novel animal model

2021 ◽  
Vol 30 (Sup6) ◽  
pp. S12-S21
Author(s):  
Diana G Sami ◽  
Ahmed Abdellatif
Keyword(s):  
Wound Healing ◽  
Drug Testing ◽  
Pressure Ulcers ◽  
Healing Process ◽  
Diabetic Rats ◽  
P Value ◽  
High Morbidity ◽  
Sprague Dawley ◽  
Patients With Diabetes ◽  
The Impact

Objective: Pressure ulcers (PUs) are a major healthcare problem, commonly associated with older people, patients who are bedbound and patients with diabetes. The impact of PUs can decrease patients' quality of life, and lead to high morbidity and mortality rates. In this study, we aimed to describe a novel PU model that simulates pressure ulcers in humans to provide a research tool for new drug testing. Method: Diabetes was induced using streptozocin in 75 adult Sprague Dawley rats. To create the PU, skin was sandwiched between two magnets, one of them implanted below the panniculus carnosus muscle and the other above the skin. The model was tested on nondiabetic rats and diabetic rats, each with pressure ulcers, compared to nondiabetic rats with excisional wounds. Results: Results showed that the PU model in diabetic (p-value<0.000001) and non-diabetic rats (p-value<0.05) exhibited significantly delayed healing (no healing over 21 days) compared with the excisional wound that was completely healed by day 21. Conclusion: Diabetic rats showed significant changes in intact skin compared with non-diabetic rats, as well as a significant delay in the healing process compared with the non-diabetic group. By effectively impairing the skin contraction otherwise seen in the rats, and thereby delaying healing and making it similar to that seen in hard-to-heal PUs in humans, this model provides an effective tool for wound healing research.

scholarly journals An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4610
Author(s):  
Hye-Jin Lee ◽  
Moses Jeong ◽  
Young-Guk Na ◽  
Sung-Jin Kim ◽  
Hong-Ki Lee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Double Emulsion ◽  
Average Diameter ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Nanostructured Lipid Carrier

Nanostructured lipid carriers (NLC) are capable of encapsulating hydrophilic and lipophilic drugs. The present study developed an NLC containing epidermal growth factor (EGF) and curcumin (EGF–Cur-NLC). EGF–Cur-NLC was prepared by a modified water-in-oil-in-water (w/o/w) double-emulsion method. The EGF–Cur-NLC particles showed an average diameter of 331.8 nm and a high encapsulation efficiency (81.1% and 99.4% for EGF and curcumin, respectively). In vitro cell studies were performed using two cell types, NIH 3T3 fibroblasts and HaCaT keratinocytes. The results showed no loss of bioactivity of EGF in the NLC formulation. In addition, EGF–Cur-NLC improved in vitro cell migration, which mimics the wound healing process. Finally, EGF–Cur-NLC was evaluated in a chronic wound model in diabetic rats. We found that EGF–Cur-NLC accelerated wound closure and increased the activity of antioxidant enzymes. Overall, these results reveal the potential of the NLC formulation containing EGF and curcumin to promote healing of chronic wounds.

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

scholarly journals Sida cordifolia Linn. accelerates wound healing process in type 2 diabetic rats

2016 ◽  
Vol 6 (4) ◽  
pp. 82-89 ◽  
Author(s):  
Rajesh Singh Pawar ◽  
Shweta Kumar ◽  
Fedelic Ashish Toppo ◽  
Lakshmi PK ◽  
Pratibha Suryavanshi
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Sida Cordifolia ◽  
Type 2 Diabetic

scholarly journals Effects of Low-Intensity Laser Irradiation on Wound Healing in Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Ma ◽  
Ying-xin Li ◽  
Hong-li Chen ◽  
Mei-ling Kang ◽  
Timon Cheng-Yi Liu
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Laser Irradiation ◽  
Healing Process ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Low Intensity ◽  
Intensity Laser ◽  
Later Phase

Objective.The effects of low-intensity 630 nm semiconductor laser irradiation at 3.6 J/cm2(LISL) on wound healing in diabetic rats were studied in this paper.Methods.36 diabetic rats with dorsal cutaneous excisional wounds were divided into three LISL groups and a control group randomly. The three LISL groups were irradiated with LISL at 5, 10, and 20 mW/cm2five times a week for two weeks, respectively. The process of wound healing was assessed by assessing blood glucose, calculating percentage of wound closure, histopathological evaluation, and immunohistochemical quantification.Results.Blood glucose of all groups remained at similar levels throughout the experiment. LISL could obviously promote wound contraction, fibroblasts proliferation, and collagen synthesis, alter bFGF and TGF-β1 expression, and reduce inflammatory reaction in the early and middle phases of chronic wound-healing process. However, LISL could not shorten cicatrization time, and the treatment effects were not sensitive to illuminate parameters in the later phase of the experiment.Conclusions.LISL might have auxiliary effects in the early and middle phases of wound healing in STZ-induced diabetic rats, but the reciprocity rule might not hold. The wound-healing process of early-phase diabetes rats shows typical characteristics of self-limited disease.

scholarly journals Effects of Sildenafil Citrate On Testicular Histology and Sperm Count During Wound Healing Process in Diabetic Rats

2019 ◽  
Vol 16 (1) ◽  
pp. 1-11
Author(s):  
Mohammed B. Mahre ◽  
Saidu I Ngulde ◽  
Bukar Umaru ◽  
Fakilahyel M Mshelbwala ◽  
Dauda Yahi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sperm Count ◽  
Healing Process ◽  
Diabetic Rats ◽  
Sildenafil Citrate ◽  
Wound Healing Process ◽  
Testicular Histology

Wound healing after tonsillectomy – a review of the literature

2018 ◽  
Vol 132 (9) ◽  
pp. 764-770 ◽  
Author(s):  
N H Davidoss ◽  
R Eikelboom ◽  
P L Friedland ◽  
P L Santa Maria
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
English Literature ◽  
Healing Process ◽  
Experimental Models ◽  
Adjunctive Treatment ◽  
Review Of The Literature ◽  
Ovid Medline ◽  
Search Terms ◽  
Post Operative Period

AbstractObjectiveTo summarise the available literature related to wound healing post tonsillectomy, including the stages of healing, experimental models for assessing healing (in animals and humans) and the various factors that affect wound healing.MethodsA search of the English literature was conducted using the Ovid Medline database, with the search terms ‘tonsillectomy’ or ‘tonsil’ and ‘wound healing’. Thirty-one articles that objectively assessed tonsillectomy wound healing were included for analysis.ResultsThe majority of assessments in humans investigating tonsillectomy wound healing involve serial direct clinical examinations of the oral cavity. Many patient and surgical factors have been shown to affect wound healing after tonsillectomy. There is some research to suggest that the administration of adjunctive treatment in the post-operative period may be beneficial to tonsillectomy wound healing.ConclusionWound healing post tonsillectomy has been poorly researched. Having a better understanding of the process of wound healing would allow surgeons to potentially prevent, anticipate and manage complications from the surgery that arise as part of the healing process.

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