scholarly journals Effects of Methanolic Extract from Turmeric (Curcuma longa) against the In Vitro Multiplication of Several Babesia Species and Theileria equi

Parasitologia ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 188-196
Author(s):  
Mohamed Abdo Rizk ◽  
Shimaa Abd El-Salam El-Sayed ◽  
Ikuo Igarashi
Keyword(s):  
Methanolic Extract ◽  
Curcuma Longa ◽  
In Vitro Growth ◽  
Theileria Equi ◽  
Diminazene Aceturate ◽  
Novel Drugs ◽  
Ic50 Values ◽  
Fluorescence Test ◽  
Novel Strategy

Anti-piroplasm drugs currently on the market have proven toxicity to the host and parasite resistance. Plants are possible sources of novel drugs. Subsequently, a novel strategy should be used to find new anti-piroplasm agents that are both effective and safe. In the present study, we have evaluated the effect of turmeric (Curcuma longa) methanolic extract on the in vitro growth of Babesia (B.) bovis, B. divergens, B. caballi, and Theileria (T.) equi. The in vitro inhibitory effectiveness of turmeric was assessed using a fluorescence test. The enhancement in the in vitro inhibitory efficacy of turmeric when administrated in combination with diminazene aceturate (DA) was investigated using in vitro cultures of different piroplasm parasites. Turmeric reduced the in vitro growth of B. bovis, B. divergens, T. equi, and B. caballi with IC50 values of 0.830 0.078, 0.375 0.055, 1.405 0.575, and 0.720 0.090 mg/mL, respectively. An amount of 1 mg/mL turmeric for B. bovis, 0.5 mg/mL turmeric for B. divergens, 1 mg/mL turmeric for T. equi, and 0.5 mg/mL turmeric for B. caballi exhibited 73.43%, 80.065%, 73.47%, and 47.375% inhibitions in the growth of the parasites, respectively. When turmeric was combined with DA, its in vitro inhibitory impact on bovine Babesia and equine Babesia/Theileria parasites was amplified. These findings show that a methanolic extract of turmeric could be a promising medicinal plant for the treatment of babesiosis, especially when administered in conjunction with DA.

scholarly journals Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1694
Author(s):  
Kamel Arraki ◽  
Perle Totoson ◽  
Alain Decendit ◽  
Andy Zedet ◽  
Justine Maroilley ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Isolation And Identification ◽  
Vasorelaxant Effect ◽  
Methanolic Extracts ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

scholarly journals Demonstration of Phytochemicals and In-vitro Antioxidant and Anti-inflammatory activity by methanolic extract of Elettaria cardamomum

2020 ◽  
Vol 3 (3) ◽  
pp. 01-04
Author(s):  
Md. Shahidul Islam
Keyword(s):  
Methanolic Extract ◽  
Research Work ◽  
Inflammatory Activity ◽  
Pharmacological Activities ◽  
Elettaria Cardamomum ◽  
Ic50 Values ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Dpph Scavenging

The existing research study attempts to untie novel avenues for development of the medicinal exercises of Elettaria cardamomum, fashionable known as the “Queen of Spices” and locally recognized as “elaichi”. Its seeds are utilized as abortifacient, acrid, alexiteric, aromatic, sweet, cardiac tonic, cooling, carminative, digestive, diuretic, expectorant, stimulant and also tonic beneficial in the asthma, haemorrhoids, bronchitis, strangury, renal in addition to vesical calculi, anorexia, halitosis, gastropathy dyspepsia as well as burning sensation. The prime goal of this research work is to evaluate antioxidant as well as anti-inflammatory properties of the traditional Bangladeshi medicinal extracts in addition to examine these activities. The aim in present work was to screen the phytochemical profile as well as pharmacological activities of the methanolic extract of this plant’s leaves. To explore pharmacological actions DPPH scavenging test and the HRBC membrane stabilization techniques were done for the antioxidant and also anti-inflammatory test respectively. The pharmacological works revealed that plant extracts might have noteworthy antioxidant effect which is possibly mediated by the inhibition of DPPH free radical which is accountable for oxidation. The IC50 values by the DPPH scavenging test observed for the standard and the leaves were 106.38µg/ml & 594.47µg/ml respectively. There is also moderate anti-inflammatory activity. The IC50 values for anti-inflammatory activity by standard & leaves were 35.04µg/ml and 944.0 µg/ml respectively.

scholarly journals Protoflavone-Chalcone Hybrids Exhibit Enhanced Antitumor Action through Modulating Redox Balance, Depolarizing the Mitochondrial Membrane, and Inhibiting ATR-Dependent Signaling

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 519 ◽  
Author(s):  
Ahmed Dhahir Latif ◽  
Tamás Jernei ◽  
Ana Podolski-Renić ◽  
Ching-Ying Kuo ◽  
Máté Vágvölgyi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mitochondrial Membrane ◽  
Redox Balance ◽  
Breast Cancer Cell Lines ◽  
Antitumor Action ◽  
Hybrid Compounds ◽  
Mitochondrial Membrane Depolarization ◽  
Ic50 Values ◽  
Novel Strategy

Hybrid compounds combine fragments with complementary targets to achieve a common pharmacological goal. This approach represents an increasingly popular strategy for drug discovery. In this work, we aimed to design antitumor hybrid compounds based on an inhibitor of ataxia-telangiectasia and Rad3-related protein (ATR)-dependent signaling, protoapigenone, and a pro-oxidant ferrocene or chalcone fragment. Four new triazole-coupled hybrids were prepared. The compounds were cytotoxic against human breast cancer cell lines in vitro, showing IC50 values in the sub-micromolar range. The nature of interactions between relevant fragments of the hybrids was evaluated by the Chou–Talalay method. Experimental combination treatment with the fragments showed additive effects or slight/moderate synergism, while strong synergism was observed when the fragments were virtually combined into their hybrids, suggesting a relevant pharmacological benefit of the coupling. All hybrids were strong inhibitors of the ATR-mediated activation of Chk1, and they interfered with the redox balance of the cells leading to mitochondrial membrane depolarization. Additionally, they induced late apoptosis and primary necrosis in MDA-MB-231 and MCF-7 breast cancer cells, respectively. Our results demonstrate that coupling the ATR-dependent signaling inhibitor protoflavone with a pro-oxidant chalcone dramatically increases the antitumor activity compared with either fragment alone. Such compounds may offer an attractive novel strategy for the treatment of various cancers.

scholarly journals In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Canthium coromandelicum Leaves

2020 ◽  
pp. 12-18
Author(s):  
S. Thiripura Salini ◽  
S. Shankar
Keyword(s):  
Diabetic Complications ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antidiabetic Activity ◽  
Dpph Radical ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Ic50 Values ◽  
In Vitro Antioxidant Activity

Oxidative stress plays a major role in diabetic complications. The study aim was to investigate the in-vitro antidiabetic and antioxidant activities of methanolic extract of Canthium coromandelicum leaves. The plant material was extracted with methanol and the methanolic extract was screened for in-vitro antioxidant activity using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) assay. The efficiency of the antidiabetic activity of the plant extract was evaluated against α-amylase and α-glucosidase digestive enzymes. The study revealed that the C. coromandelicum extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 31.52 ± 0.42 and 41.49 ± 0.28 µg/mL respectively and compared with standard acarbose drug. The extract efficiently scavenging DPPH radical with IC50 values of 65.46 ± 0.50 µg/ml. Therefore, the extract could be a promising therapeutic in management of diabetic complications.

In vitro growth inhibitory efficacy of some target specific novel drug molecules against Theileria equi

2016 ◽  
Vol 217 ◽  
pp. 1-6 ◽  
Author(s):  
A. Gopalakrishnan ◽  
C. Maji ◽  
R.K. Dahiya ◽  
A. Suthar ◽  
R. Kumar ◽  
...  
Keyword(s):  
In Vitro Growth ◽  
Theileria Equi ◽  
Drug Molecules ◽  
Growth Inhibitory ◽  
Novel Drug

scholarly journals Inhibition of the in vitro growth of Babesia bigemina, Babesia caballi and Theileria equi parasites by trifluralin analogues

2017 ◽  
Vol 8 (4) ◽  
pp. 593-597 ◽  
Author(s):  
Marta G. Silva ◽  
Donald P. Knowles ◽  
Sandra Antunes ◽  
Ana Domingos ◽  
Maria A. Esteves ◽  
...  
Keyword(s):  
In Vitro Growth ◽  
Babesia Bigemina ◽  
Theileria Equi ◽  
Babesia Caballi

scholarly journals α-Glucosidase Inhibitory and Antiradical Properties of Acacia macrostachya

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Hamidou Têeda Ganamé ◽  
Yssouf Karanga ◽  
Ousmane Ilboudo ◽  
Wende-Konté Hazael Conania Nikiema ◽  
Richard Wamtinga Sawadogo ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Antiradical Activity ◽  
Methanolic Extract ◽  
Inhibitory Effect ◽  
Traditional Use ◽  
Methanol Extracts ◽  
Antiradical Properties ◽  
Ic50 Values ◽  
Vitro Model

In this work, the anti-diabetic activity of three extracts of Acacia macrostachya was investigated by following the inhibitory effect of these extracts on -glucosidase using the in vitro model. The antiradical activity of these extracts was also determined. Methanol extracts of root and stem barks showed a very significant inhibitory effect against the enzyme activity of -glucosidase with IC50 2.487 ± 0.441 µg/mL and 1.650 ± 0.229 µg/mL respectively. For antiradical activity, the same extracts presented the highest scavenging of the radical DPPH● with IC50 values of 9.307 ± 0.262 µg/mL and 5.242 ± 0.068 µg/mL respectively. With the cationic radical ABTS●+, IC50 varied from 45.049 ± 0.730 µg/mL for methanolic root barks extract to 14.136 ± 0.161 µg/mL for methanolic extract from stem barks. Thus, the methanol extracts of the root and stem barks of Acacia macrostachya possess compounds with very interesting anti-diabetic and antiradical properties and could justify its traditional use.

scholarly journals Evaluation of the Inhibitory Effects of Coumermycin A1 on the Growth of Theileria and Babesia Parasites in vitro and in vivo

2021 ◽  
Vol 41 (04) ◽  
pp. 469-474
Author(s):  
Mahmoud AbouLaila
Keyword(s):  
Dna Gyrase ◽  
Babesia Microti ◽  
Babesia Species ◽  
Inhibitory Effects ◽  
Theileria Equi ◽  
Dna Topoisomerase ◽  
Ic50 Values ◽  
Gyrase A

Coumermycin A1, a coumarin antibiotic, has anticancer, antibacterial, antiviral, and antimalarial activities. We aimed to evaluate the anti-thielerial and anti-babesial activity of coumermycin A1 in mice in vivo. Coumermycin A1 efficacy was determined by the transcription of DNA gyrase, a type II DNA topoisomerase using reverse transcriptase-polymerase chain reaction (RT-PCR) transcription. Coumermycin A1 significantly inhibited the development of preliminary parasitemia (1%). Theileria equi and the Babesia species B. bigemina, B. bovis, and B. caballi were observed with IC50 values of 80, 70, 57, and 65 nM, respectively. Their development was remarkably inhibited at observed concentrations of 10, 25, 50, and 100µM for the studied organisms T. equi, and the Babesia species B. caballi, B. bovis and B. bigemina, respectively. In the subsequent viability test, parasite re-growth was suppressed at 100µM for B. bigemina and B. bovis and at 50 µM for B. caballi and T. equi. Coumermycin A1 Treatment of B. bovis cultures with Coumermycin A1 completely suppressed the transcription of the DNA gyrase subunits B and A genes. In BALB/c mice, the development of Babesia microti was inhibited by 70.73% using 5 mg/kg of Coumermycin A1.

scholarly journals Curcumin Analogues with Aldose Reductase Inhibitory Activity: Synthesis, Biological Evaluation, and Molecular Docking

Processes ◽  
2019 ◽  
Vol 7 (7) ◽  
pp. 417 ◽  
Author(s):  
Dasharath Kondhare ◽  
Sushma Deshmukh ◽  
Harshad Lade
Keyword(s):  
Molecular Docking ◽  
Aldose Reductase ◽  
Diabetic Complications ◽  
Curcuma Longa ◽  
Polyol Pathway ◽  
Biological Evaluation ◽  
Curcumin Analogues ◽  
Ic50 Values ◽  
Rate Limiting

Curcumin, a constituent of Curcuma longa, has shown numerous biological and pharmacological activities, including antidiabetic effects. Here, a novel series of curcumin analogues were synthesized and evaluated for in vitro inhibition of aldose reductase (AR), the first and rate-limiting enzyme of the polyol pathway, which plays a key role in the onset and progression of diabetic complications. Biological activity studies showed that all the curcuminoids exhibited moderate to good AR inhibitory (ARI) activities compared with that of the quercetin standard. Importantly, compounds 8d, 8h, 9c, 9e, and 10g demonstrated promising ARI activities, with the 50% inhibitory concentration (IC50) values of 5.73, 5.95, 5.11, 5.78, and 5.10 µM, respectively. Four other compounds exhibited IC50 values in the range of 6.04–6.18 µM. Methyl and methoxy derivatives showed a remarkable ARI potential compared with that of other substitutions on the aromatic ring. Molecular docking experiments demonstrated that the most active curcuminoid (10g) was able to favorably bind in the active site of the AR enzyme. The potent ARI activities exhibited by the curcuminoids were attributed to their substitution patterns on the aromatic moiety, which may provide novel leads in the development of therapeutics for the treatment of diabetic complications.

Sign in / Sign up

Export Citation Format

Share Document